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P02918

- PBPA_ECOLI

UniProt

P02918 - PBPA_ECOLI

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Protein

Penicillin-binding protein 1A

Gene
mrcA, ponA, b3396, JW3359
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei86 – 861Proton donor; for transglycosylase activity Reviewed prediction
Active sitei465 – 4651Acyl-ester intermediate; for transpeptidase activity

GO - Molecular functioni

  1. penicillin binding Source: EcoCyc
  2. peptidoglycan glycosyltransferase activity Source: EcoCyc
  3. protein binding Source: IntAct
  4. serine-type D-Ala-D-Ala carboxypeptidase activity Source: EcoCyc
  5. transferase activity, transferring pentosyl groups Source: InterPro

GO - Biological processi

  1. negative regulation of cytolysis Source: EcoCyc
  2. peptidoglycan biosynthetic process Source: EcoCyc
  3. proteolysis Source: GOC
  4. regulation of cell shape Source: EcoCyc
  5. response to antibiotic Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Hydrolase, Transferase

Keywords - Biological processi

Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG10748-MONOMER.
ECOL316407:JW3359-MONOMER.
MetaCyc:EG10748-MONOMER.
UniPathwayiUPA00219.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 1A
Short name:
PBP-1a
Short name:
PBP1a
Including the following 2 domains:
Penicillin-insensitive transglycosylase (EC:2.4.2.-)
Alternative name(s):
Peptidoglycan TGase
Penicillin-sensitive transpeptidase (EC:3.4.-.-)
Alternative name(s):
DD-transpeptidase
Gene namesi
Name:mrcA
Synonyms:ponA
Ordered Locus Names:b3396, JW3359
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10748. mrcA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 55Cytoplasmic Reviewed prediction
Transmembranei6 – 2621Helical; Signal-anchor for type II membrane protein; Reviewed predictionAdd
BLAST
Topological domaini27 – 850824Periplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 850850Penicillin-binding protein 1APRO_0000083164Add
BLAST

Proteomic databases

PaxDbiP02918.
PRIDEiP02918.

Expressioni

Gene expression databases

GenevestigatoriP02918.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
lpoAP454644EBI-1126191,EBI-557795

Protein-protein interaction databases

DIPiDIP-10251N.
IntActiP02918. 8 interactions.
STRINGi511145.b3396.

Structurei

3D structure databases

ProteinModelPortaliP02918.
SMRiP02918. Positions 47-789.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni48 – 216169TransglycosylaseAdd
BLAST
Regioni400 – 710311TranspeptidaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 51 family.
In the C-terminal section; belongs to the transpeptidase family.

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5009.
HOGENOMiHOG000041138.
KOiK05366.
OMAiMEIATGW.
OrthoDBiEOG6HMXCD.
PhylomeDBiP02918.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.710.10. 3 hits.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR012340. NA-bd_OB-fold.
IPR011816. PBP_1a.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 4 hits.
TIGRFAMsiTIGR02074. PBP_1a_fam. 1 hit.

Sequencei

Sequence statusi: Complete.

P02918-1 [UniParc]FASTAAdd to Basket

« Hide

MKFVKYFLIL AVCCILLGAG SIYGLYRYIE PQLPDVATLK DVRLQIPMQI    50
YSADGELIAQ YGEKRRIPVT LDQIPPEMVK AFIATEDSRF YEHHGVDPVG 100
IFRAASVALF SGHASQGAST ITQQLARNFF LSPERTLMRK IKEVFLAIRI 150
EQLLTKDEIL ELYLNKIYLG YRAYGVGAAA QVYFGKTVDQ LTLNEMAVIA 200
GLPKAPSTFN PLYSMDRAVA RRNVVLSRML DEGYITQQQF DQTRTEAINA 250
NYHAPEIAFS APYLSEMVRQ EMYNRYGESA YEDGYRIYTT ITRKVQQAAQ 300
QAVRNNVLDY DMRHGYRGPA NVLWKVGESA WDNNKITDTL KALPTYGPLL 350
PAAVTSANPQ QATAMLADGS TVALSMEGVR WARPYRSDTQ QGPTPRKVTD 400
VLQTGQQIWV RQVGDAWWLA QVPEVNSALV SINPQNGAVM ALVGGFDFNQ 450
SKFNRATQAL RQVGSNIKPF LYTAAMDKGL TLASMLNDVP ISRWDASAGS 500
DWQPKNSPPQ YAGPIRLRQG LGQSKNVVMV RAMRAMGVDY AAEYLQRFGF 550
PAQNIVHTES LALGSASFTP MQVARGYAVM ANGGFLVDPW FISKIENDQG 600
GVIFEAKPKV ACPECDIPVI YGDTQKSNVL ENNDVEDVAI SREQQNVSVP 650
MPQLEQANQA LVAKTGAQEY APHVINTPLA FLIKSALNTN IFGEPGWQGT 700
GWRAGRDLQR RDIGGKTGTT NSSKDAWFSG YGPGVVTSVW IGFDDHRRNL 750
GHTTASGAIK DQISGYEGGA KSAQPAWDAY MKAVLEGVPE QPLTPPPGIV 800
TVNIDRSTGQ LANGGNSREE YFIEGTQPTQ QAVHEVGTTI IDNGEAQELF 850
Length:850
Mass (Da):93,636
Last modified:July 21, 1986 - v1
Checksum:i0A54C6B28783E34B
GO

Sequence cautioni

The sequence AAA58193.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02164 Genomic DNA. Translation: CAA26100.1.
U18997 Genomic DNA. Translation: AAA58193.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76421.2.
AP009048 Genomic DNA. Translation: BAE77895.1.
PIRiG65134. ZPECPA.
RefSeqiNP_417855.4. NC_000913.3.
YP_492036.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76421; AAC76421; b3396.
BAE77895; BAE77895; BAE77895.
GeneIDi12933998.
947907.
KEGGiecj:Y75_p3780.
eco:b3396.
PATRICi32122224. VBIEscCol129921_3489.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02164 Genomic DNA. Translation: CAA26100.1 .
U18997 Genomic DNA. Translation: AAA58193.1 . Different initiation.
U00096 Genomic DNA. Translation: AAC76421.2 .
AP009048 Genomic DNA. Translation: BAE77895.1 .
PIRi G65134. ZPECPA.
RefSeqi NP_417855.4. NC_000913.3.
YP_492036.1. NC_007779.1.

3D structure databases

ProteinModelPortali P02918.
SMRi P02918. Positions 47-789.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-10251N.
IntActi P02918. 8 interactions.
STRINGi 511145.b3396.

Chemistry

ChEMBLi CHEMBL2354204.
DrugBanki DB01413. Cefepime.
DB00303. Ertapenem.

Protein family/group databases

CAZyi GT51. Glycosyltransferase Family 51.

Proteomic databases

PaxDbi P02918.
PRIDEi P02918.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76421 ; AAC76421 ; b3396 .
BAE77895 ; BAE77895 ; BAE77895 .
GeneIDi 12933998.
947907.
KEGGi ecj:Y75_p3780.
eco:b3396.
PATRICi 32122224. VBIEscCol129921_3489.

Organism-specific databases

EchoBASEi EB0741.
EcoGenei EG10748. mrcA.

Phylogenomic databases

eggNOGi COG5009.
HOGENOMi HOG000041138.
KOi K05366.
OMAi MEIATGW.
OrthoDBi EOG6HMXCD.
PhylomeDBi P02918.

Enzyme and pathway databases

UniPathwayi UPA00219 .
BioCyci EcoCyc:EG10748-MONOMER.
ECOL316407:JW3359-MONOMER.
MetaCyc:EG10748-MONOMER.

Miscellaneous databases

PROi P02918.

Gene expression databases

Genevestigatori P02918.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
3.40.710.10. 3 hits.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR012340. NA-bd_OB-fold.
IPR011816. PBP_1a.
IPR001460. PCN-bd_Tpept.
[Graphical view ]
Pfami PF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view ]
SUPFAMi SSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 4 hits.
TIGRFAMsi TIGR02074. PBP_1a_fam. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequences of the ponA and ponB genes encoding penicillin-binding protein 1A and 1B of Escherichia coli K12."
    Broome-Smith J.K., Edelman A., Yousif S., Spratt B.G.
    Eur. J. Biochem. 147:437-446(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Sequences of the active-site peptides of three of the high-Mr penicillin-binding proteins of Escherichia coli K-12."
    Keck W., Glauner B., Schwarz U., Broome-Smith J.K., Spratt B.G.
    Proc. Natl. Acad. Sci. U.S.A. 82:1999-2003(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 452-480.
    Strain: K12.
  5. "Dual enzyme activities of cell wall peptidoglycan synthesis, peptidoglycan transglycosylase and penicillin-sensitive transpeptidase, in purified preparations of Escherichia coli penicillin-binding protein 1A."
    Ishino F., Mitsui K., Tamaki S., Matsuhashi M.
    Biochem. Biophys. Res. Commun. 97:287-293(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: K12.
  6. "Multimodular penicillin-binding proteins: an enigmatic family of orthologs and paralogs."
    Goffin C., Ghuysen J.-M.
    Microbiol. Mol. Biol. Rev. 62:1079-1093(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiPBPA_ECOLI
AccessioniPrimary (citable) accession number: P02918
Secondary accession number(s): P76688, Q2M761
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 11, 2014
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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