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P02918

- PBPA_ECOLI

UniProt

P02918 - PBPA_ECOLI

Protein

Penicillin-binding protein 1A

Gene

mrcA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 149 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei86 – 861Proton donor; for transglycosylase activitySequence Analysis
    Active sitei465 – 4651Acyl-ester intermediate; for transpeptidase activity

    GO - Molecular functioni

    1. penicillin binding Source: EcoCyc
    2. peptidoglycan glycosyltransferase activity Source: EcoCyc
    3. protein binding Source: IntAct
    4. serine-type D-Ala-D-Ala carboxypeptidase activity Source: EcoCyc
    5. transferase activity, transferring pentosyl groups Source: InterPro

    GO - Biological processi

    1. negative regulation of cytolysis Source: EcoCyc
    2. peptidoglycan biosynthetic process Source: EcoCyc
    3. proteolysis Source: GOC
    4. regulation of cell shape Source: EcoCyc
    5. response to antibiotic Source: EcoCyc

    Keywords - Molecular functioni

    Glycosyltransferase, Hydrolase, Transferase

    Keywords - Biological processi

    Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10748-MONOMER.
    ECOL316407:JW3359-MONOMER.
    MetaCyc:EG10748-MONOMER.
    UniPathwayiUPA00219.

    Protein family/group databases

    CAZyiGT51. Glycosyltransferase Family 51.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Penicillin-binding protein 1A
    Short name:
    PBP-1a
    Short name:
    PBP1a
    Including the following 2 domains:
    Penicillin-insensitive transglycosylase (EC:2.4.2.-)
    Alternative name(s):
    Peptidoglycan TGase
    Penicillin-sensitive transpeptidase (EC:3.4.-.-)
    Alternative name(s):
    DD-transpeptidase
    Gene namesi
    Name:mrcA
    Synonyms:ponA
    Ordered Locus Names:b3396, JW3359
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10748. mrcA.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of plasma membrane Source: EcoliWiki

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 850850Penicillin-binding protein 1APRO_0000083164Add
    BLAST

    Proteomic databases

    PaxDbiP02918.
    PRIDEiP02918.

    Expressioni

    Gene expression databases

    GenevestigatoriP02918.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    lpoAP454644EBI-1126191,EBI-557795

    Protein-protein interaction databases

    DIPiDIP-10251N.
    IntActiP02918. 8 interactions.
    STRINGi511145.b3396.

    Structurei

    3D structure databases

    ProteinModelPortaliP02918.
    SMRiP02918. Positions 47-789.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 55CytoplasmicSequence Analysis
    Topological domaini27 – 850824PeriplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei6 – 2621Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni48 – 216169TransglycosylaseAdd
    BLAST
    Regioni400 – 710311TranspeptidaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the glycosyltransferase 51 family.Curated
    In the C-terminal section; belongs to the transpeptidase family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5009.
    HOGENOMiHOG000041138.
    KOiK05366.
    OMAiMEIATGW.
    OrthoDBiEOG6HMXCD.
    PhylomeDBiP02918.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.40.710.10. 3 hits.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR001264. Glyco_trans_51.
    IPR023346. Lysozyme-like_dom.
    IPR012340. NA-bd_OB-fold.
    IPR011816. PBP_1a.
    IPR001460. PCN-bd_Tpept.
    [Graphical view]
    PfamiPF00912. Transgly. 1 hit.
    PF00905. Transpeptidase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53955. SSF53955. 1 hit.
    SSF56601. SSF56601. 4 hits.
    TIGRFAMsiTIGR02074. PBP_1a_fam. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P02918-1 [UniParc]FASTAAdd to Basket

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    MKFVKYFLIL AVCCILLGAG SIYGLYRYIE PQLPDVATLK DVRLQIPMQI    50
    YSADGELIAQ YGEKRRIPVT LDQIPPEMVK AFIATEDSRF YEHHGVDPVG 100
    IFRAASVALF SGHASQGAST ITQQLARNFF LSPERTLMRK IKEVFLAIRI 150
    EQLLTKDEIL ELYLNKIYLG YRAYGVGAAA QVYFGKTVDQ LTLNEMAVIA 200
    GLPKAPSTFN PLYSMDRAVA RRNVVLSRML DEGYITQQQF DQTRTEAINA 250
    NYHAPEIAFS APYLSEMVRQ EMYNRYGESA YEDGYRIYTT ITRKVQQAAQ 300
    QAVRNNVLDY DMRHGYRGPA NVLWKVGESA WDNNKITDTL KALPTYGPLL 350
    PAAVTSANPQ QATAMLADGS TVALSMEGVR WARPYRSDTQ QGPTPRKVTD 400
    VLQTGQQIWV RQVGDAWWLA QVPEVNSALV SINPQNGAVM ALVGGFDFNQ 450
    SKFNRATQAL RQVGSNIKPF LYTAAMDKGL TLASMLNDVP ISRWDASAGS 500
    DWQPKNSPPQ YAGPIRLRQG LGQSKNVVMV RAMRAMGVDY AAEYLQRFGF 550
    PAQNIVHTES LALGSASFTP MQVARGYAVM ANGGFLVDPW FISKIENDQG 600
    GVIFEAKPKV ACPECDIPVI YGDTQKSNVL ENNDVEDVAI SREQQNVSVP 650
    MPQLEQANQA LVAKTGAQEY APHVINTPLA FLIKSALNTN IFGEPGWQGT 700
    GWRAGRDLQR RDIGGKTGTT NSSKDAWFSG YGPGVVTSVW IGFDDHRRNL 750
    GHTTASGAIK DQISGYEGGA KSAQPAWDAY MKAVLEGVPE QPLTPPPGIV 800
    TVNIDRSTGQ LANGGNSREE YFIEGTQPTQ QAVHEVGTTI IDNGEAQELF 850
    Length:850
    Mass (Da):93,636
    Last modified:July 21, 1986 - v1
    Checksum:i0A54C6B28783E34B
    GO

    Sequence cautioni

    The sequence AAA58193.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02164 Genomic DNA. Translation: CAA26100.1.
    U18997 Genomic DNA. Translation: AAA58193.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC76421.2.
    AP009048 Genomic DNA. Translation: BAE77895.1.
    PIRiG65134. ZPECPA.
    RefSeqiNP_417855.4. NC_000913.3.
    YP_492036.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76421; AAC76421; b3396.
    BAE77895; BAE77895; BAE77895.
    GeneIDi12933998.
    947907.
    KEGGiecj:Y75_p3780.
    eco:b3396.
    PATRICi32122224. VBIEscCol129921_3489.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02164 Genomic DNA. Translation: CAA26100.1 .
    U18997 Genomic DNA. Translation: AAA58193.1 . Different initiation.
    U00096 Genomic DNA. Translation: AAC76421.2 .
    AP009048 Genomic DNA. Translation: BAE77895.1 .
    PIRi G65134. ZPECPA.
    RefSeqi NP_417855.4. NC_000913.3.
    YP_492036.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P02918.
    SMRi P02918. Positions 47-789.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-10251N.
    IntActi P02918. 8 interactions.
    STRINGi 511145.b3396.

    Chemistry

    ChEMBLi CHEMBL2354204.
    DrugBanki DB01413. Cefepime.
    DB00303. Ertapenem.

    Protein family/group databases

    CAZyi GT51. Glycosyltransferase Family 51.

    Proteomic databases

    PaxDbi P02918.
    PRIDEi P02918.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76421 ; AAC76421 ; b3396 .
    BAE77895 ; BAE77895 ; BAE77895 .
    GeneIDi 12933998.
    947907.
    KEGGi ecj:Y75_p3780.
    eco:b3396.
    PATRICi 32122224. VBIEscCol129921_3489.

    Organism-specific databases

    EchoBASEi EB0741.
    EcoGenei EG10748. mrcA.

    Phylogenomic databases

    eggNOGi COG5009.
    HOGENOMi HOG000041138.
    KOi K05366.
    OMAi MEIATGW.
    OrthoDBi EOG6HMXCD.
    PhylomeDBi P02918.

    Enzyme and pathway databases

    UniPathwayi UPA00219 .
    BioCyci EcoCyc:EG10748-MONOMER.
    ECOL316407:JW3359-MONOMER.
    MetaCyc:EG10748-MONOMER.

    Miscellaneous databases

    PROi P02918.

    Gene expression databases

    Genevestigatori P02918.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.40.710.10. 3 hits.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR001264. Glyco_trans_51.
    IPR023346. Lysozyme-like_dom.
    IPR012340. NA-bd_OB-fold.
    IPR011816. PBP_1a.
    IPR001460. PCN-bd_Tpept.
    [Graphical view ]
    Pfami PF00912. Transgly. 1 hit.
    PF00905. Transpeptidase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53955. SSF53955. 1 hit.
    SSF56601. SSF56601. 4 hits.
    TIGRFAMsi TIGR02074. PBP_1a_fam. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequences of the ponA and ponB genes encoding penicillin-binding protein 1A and 1B of Escherichia coli K12."
      Broome-Smith J.K., Edelman A., Yousif S., Spratt B.G.
      Eur. J. Biochem. 147:437-446(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Sequences of the active-site peptides of three of the high-Mr penicillin-binding proteins of Escherichia coli K-12."
      Keck W., Glauner B., Schwarz U., Broome-Smith J.K., Spratt B.G.
      Proc. Natl. Acad. Sci. U.S.A. 82:1999-2003(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 452-480.
      Strain: K12.
    5. "Dual enzyme activities of cell wall peptidoglycan synthesis, peptidoglycan transglycosylase and penicillin-sensitive transpeptidase, in purified preparations of Escherichia coli penicillin-binding protein 1A."
      Ishino F., Mitsui K., Tamaki S., Matsuhashi M.
      Biochem. Biophys. Res. Commun. 97:287-293(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: K12.
    6. "Multimodular penicillin-binding proteins: an enigmatic family of orthologs and paralogs."
      Goffin C., Ghuysen J.-M.
      Microbiol. Mol. Biol. Rev. 62:1079-1093(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiPBPA_ECOLI
    AccessioniPrimary (citable) accession number: P02918
    Secondary accession number(s): P76688, Q2M761
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 149 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3