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Protein

Penicillin-binding protein 1A

Gene

mrcA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei86 – 861Proton donor; for transglycosylase activitySequence Analysis
Active sitei465 – 4651Acyl-ester intermediate; for transpeptidase activity

GO - Molecular functioni

  1. penicillin binding Source: EcoCyc
  2. peptidoglycan glycosyltransferase activity Source: EcoCyc
  3. serine-type D-Ala-D-Ala carboxypeptidase activity Source: EcoCyc
  4. transferase activity, transferring pentosyl groups Source: InterPro

GO - Biological processi

  1. cell wall organization Source: UniProtKB-KW
  2. negative regulation of cytolysis Source: EcoCyc
  3. peptidoglycan biosynthetic process Source: EcoCyc
  4. proteolysis Source: GOC
  5. regulation of cell shape Source: EcoCyc
  6. response to antibiotic Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Hydrolase, Transferase

Keywords - Biological processi

Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG10748-MONOMER.
ECOL316407:JW3359-MONOMER.
MetaCyc:EG10748-MONOMER.
UniPathwayiUPA00219.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 1A
Short name:
PBP-1a
Short name:
PBP1a
Including the following 2 domains:
Penicillin-insensitive transglycosylase (EC:2.4.2.-)
Alternative name(s):
Peptidoglycan TGase
Penicillin-sensitive transpeptidase (EC:3.4.-.-)
Alternative name(s):
DD-transpeptidase
Gene namesi
Name:mrcA
Synonyms:ponA
Ordered Locus Names:b3396, JW3359
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10748. mrcA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 55CytoplasmicSequence Analysis
Transmembranei6 – 2621Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini27 – 850824PeriplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 850850Penicillin-binding protein 1APRO_0000083164Add
BLAST

Proteomic databases

PaxDbiP02918.
PRIDEiP02918.

Expressioni

Gene expression databases

GenevestigatoriP02918.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
lpoAP454644EBI-1126191,EBI-557795

Protein-protein interaction databases

DIPiDIP-10251N.
IntActiP02918. 8 interactions.
STRINGi511145.b3396.

Structurei

3D structure databases

ProteinModelPortaliP02918.
SMRiP02918. Positions 47-789.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni48 – 216169TransglycosylaseAdd
BLAST
Regioni400 – 710311TranspeptidaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 51 family.Curated
In the C-terminal section; belongs to the transpeptidase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5009.
HOGENOMiHOG000041138.
InParanoidiP02918.
KOiK05366.
OMAiHYGVDPS.
OrthoDBiEOG6HMXCD.
PhylomeDBiP02918.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.710.10. 3 hits.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR012340. NA-bd_OB-fold.
IPR011816. PBP_1a.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 4 hits.
TIGRFAMsiTIGR02074. PBP_1a_fam. 1 hit.

Sequencei

Sequence statusi: Complete.

P02918-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFVKYFLIL AVCCILLGAG SIYGLYRYIE PQLPDVATLK DVRLQIPMQI
60 70 80 90 100
YSADGELIAQ YGEKRRIPVT LDQIPPEMVK AFIATEDSRF YEHHGVDPVG
110 120 130 140 150
IFRAASVALF SGHASQGAST ITQQLARNFF LSPERTLMRK IKEVFLAIRI
160 170 180 190 200
EQLLTKDEIL ELYLNKIYLG YRAYGVGAAA QVYFGKTVDQ LTLNEMAVIA
210 220 230 240 250
GLPKAPSTFN PLYSMDRAVA RRNVVLSRML DEGYITQQQF DQTRTEAINA
260 270 280 290 300
NYHAPEIAFS APYLSEMVRQ EMYNRYGESA YEDGYRIYTT ITRKVQQAAQ
310 320 330 340 350
QAVRNNVLDY DMRHGYRGPA NVLWKVGESA WDNNKITDTL KALPTYGPLL
360 370 380 390 400
PAAVTSANPQ QATAMLADGS TVALSMEGVR WARPYRSDTQ QGPTPRKVTD
410 420 430 440 450
VLQTGQQIWV RQVGDAWWLA QVPEVNSALV SINPQNGAVM ALVGGFDFNQ
460 470 480 490 500
SKFNRATQAL RQVGSNIKPF LYTAAMDKGL TLASMLNDVP ISRWDASAGS
510 520 530 540 550
DWQPKNSPPQ YAGPIRLRQG LGQSKNVVMV RAMRAMGVDY AAEYLQRFGF
560 570 580 590 600
PAQNIVHTES LALGSASFTP MQVARGYAVM ANGGFLVDPW FISKIENDQG
610 620 630 640 650
GVIFEAKPKV ACPECDIPVI YGDTQKSNVL ENNDVEDVAI SREQQNVSVP
660 670 680 690 700
MPQLEQANQA LVAKTGAQEY APHVINTPLA FLIKSALNTN IFGEPGWQGT
710 720 730 740 750
GWRAGRDLQR RDIGGKTGTT NSSKDAWFSG YGPGVVTSVW IGFDDHRRNL
760 770 780 790 800
GHTTASGAIK DQISGYEGGA KSAQPAWDAY MKAVLEGVPE QPLTPPPGIV
810 820 830 840 850
TVNIDRSTGQ LANGGNSREE YFIEGTQPTQ QAVHEVGTTI IDNGEAQELF
Length:850
Mass (Da):93,636
Last modified:July 20, 1986 - v1
Checksum:i0A54C6B28783E34B
GO

Sequence cautioni

The sequence AAA58193.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02164 Genomic DNA. Translation: CAA26100.1.
U18997 Genomic DNA. Translation: AAA58193.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76421.2.
AP009048 Genomic DNA. Translation: BAE77895.1.
PIRiG65134. ZPECPA.
RefSeqiNP_417855.4. NC_000913.3.
YP_492036.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76421; AAC76421; b3396.
BAE77895; BAE77895; BAE77895.
GeneIDi12933998.
947907.
KEGGiecj:Y75_p3780.
eco:b3396.
PATRICi32122224. VBIEscCol129921_3489.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02164 Genomic DNA. Translation: CAA26100.1.
U18997 Genomic DNA. Translation: AAA58193.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76421.2.
AP009048 Genomic DNA. Translation: BAE77895.1.
PIRiG65134. ZPECPA.
RefSeqiNP_417855.4. NC_000913.3.
YP_492036.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP02918.
SMRiP02918. Positions 47-789.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-10251N.
IntActiP02918. 8 interactions.
STRINGi511145.b3396.

Chemistry

ChEMBLiCHEMBL2354204.
DrugBankiDB01414. Cefacetrile.
DB01327. Cefazolin.
DB00274. Cefmetazole.
DB01328. Cefonicid.
DB01329. Cefoperazone.
DB01331. Cefoxitin.
DB00430. Cefpiramide.
DB01333. Cefradine.
DB00438. Ceftazidime.
DB01415. Ceftibuten.
DB01332. Ceftizoxime.
DB00303. Ertapenem.
DB01598. Imipenem.
DB04570. Latamoxef.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Proteomic databases

PaxDbiP02918.
PRIDEiP02918.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76421; AAC76421; b3396.
BAE77895; BAE77895; BAE77895.
GeneIDi12933998.
947907.
KEGGiecj:Y75_p3780.
eco:b3396.
PATRICi32122224. VBIEscCol129921_3489.

Organism-specific databases

EchoBASEiEB0741.
EcoGeneiEG10748. mrcA.

Phylogenomic databases

eggNOGiCOG5009.
HOGENOMiHOG000041138.
InParanoidiP02918.
KOiK05366.
OMAiHYGVDPS.
OrthoDBiEOG6HMXCD.
PhylomeDBiP02918.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciEcoCyc:EG10748-MONOMER.
ECOL316407:JW3359-MONOMER.
MetaCyc:EG10748-MONOMER.

Miscellaneous databases

PROiP02918.

Gene expression databases

GenevestigatoriP02918.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.710.10. 3 hits.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR012340. NA-bd_OB-fold.
IPR011816. PBP_1a.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 4 hits.
TIGRFAMsiTIGR02074. PBP_1a_fam. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequences of the ponA and ponB genes encoding penicillin-binding protein 1A and 1B of Escherichia coli K12."
    Broome-Smith J.K., Edelman A., Yousif S., Spratt B.G.
    Eur. J. Biochem. 147:437-446(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Sequences of the active-site peptides of three of the high-Mr penicillin-binding proteins of Escherichia coli K-12."
    Keck W., Glauner B., Schwarz U., Broome-Smith J.K., Spratt B.G.
    Proc. Natl. Acad. Sci. U.S.A. 82:1999-2003(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 452-480.
    Strain: K12.
  5. "Dual enzyme activities of cell wall peptidoglycan synthesis, peptidoglycan transglycosylase and penicillin-sensitive transpeptidase, in purified preparations of Escherichia coli penicillin-binding protein 1A."
    Ishino F., Mitsui K., Tamaki S., Matsuhashi M.
    Biochem. Biophys. Res. Commun. 97:287-293(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: K12.
  6. "Multimodular penicillin-binding proteins: an enigmatic family of orthologs and paralogs."
    Goffin C., Ghuysen J.-M.
    Microbiol. Mol. Biol. Rev. 62:1079-1093(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiPBPA_ECOLI
AccessioniPrimary (citable) accession number: P02918
Secondary accession number(s): P76688, Q2M761
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 20, 1986
Last sequence update: July 20, 1986
Last modified: January 6, 2015
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.