P02887 (DIS1B_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Discoidin-1 subunit B/C

Alternative name(s):
Discoidin I chain C/D
Discoidin-1 subunit beta/gamma

Gene names

Name:	dscC-1
Synonyms:	dscB-1
ORF Names:	DDB_G0273065
AND
Name:	dscC-2
Synonyms:	dscB-2
ORF Names:	DDB_G0273885

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Galactose- and N-acetylgalactosamine-binding lectin. May play a role in cell-substratum adhesion rather than in cell-cell adhesion. May be necessary for the maintenance of normal elongate morphology during aggregation.
Subunit structure	Tetramer of four different chains (A to D).
Subcellular location	Cytoplasm.
Tissue specificity	Stalk cells.
Sequence similarities	Contains 1 F5/8 type C domain.
Caution	The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cytoplasm
Ligand	Lectin
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	phagocytic vesicle Inferred from direct assay Ref.6. Source: dictyBase
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 253	252	Discoidin-1 subunit B/C		PRO_0000079917
Regions
	Domain	2 – 152	151	F5/8 type C
	Motif	79 – 81	3	Cell attachment site
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P02887 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 875EA94811F85444	FASTA	253	28,392
	10 20 30 40 50 60 MSTQGLVQLI SNAQCHLRTS TNYNDVHTQF NAVLNYKNKG TNTIDGSEAW CSSIVDTNQY 70 80 90 100 110 120 IVAGCEVPRT FMCVALQGRG DHDQWVTSYK IRYSLDNVTW SEYRNGAAIT GVTDRNTVVN 130 140 150 160 170 180 HFFDTPIRAR SIAIHPLTWN NHISLRCEFY TQPVQSSVTQ VGADIYTGDN CALNTGSGKR 190 200 210 220 230 240 EVVVPVKFQF EFATLPKVAL NFDQIDCTDA TNQTRIGVQP RNITTKGFDC VFYTWNANKV 250 YSLRADYIAT ALE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and expression of the discoidin I gene family in Dictyostelium discoideum." Poole S., Firtel R.A., Lamar E., Rowekamp W. J. Mol. Biol. 153:273-289(1981) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[4]	Bienvenut W.V., Patel H., Brunton V.G., Frame M.C. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-18; 70-79; 105-128; 131-146 AND 188-197, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, MASS SPECTROMETRY.
[5]	"Discoidin I is implicated in cell-substratum attachment and ordered cell migration of Dictyostelium discoideum and resembles fibronectin." Springer W.R., Cooper D.N.W., Barondes S.H. Cell 39:557-564(1984) [PubMed] [Europe PMC] [Abstract] Cited for: CELL ATTACHMENT SITE.
[6]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: AX2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J01284 Genomic DNA. Translation: AAA33199.1. J01283 Genomic DNA. Translation: AAA33198.1. AAFI02000011 Genomic DNA. Translation: EAL70633.1. AAFI02000009 Genomic DNA. Translation: EAL70750.1.
PIR	DLDOIC. A03382. B03382.
RefSeq	XP_644559.1. XM_639467.1. XP_644692.1. XM_639600.1.
3D structure databases
ProteinModelPortal	P02887.
ModBase	Search...
2D gel databases
SWISS-2DPAGE	P02887.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0215401; DDB0215401; DDB_G0273065. DDB0266624; DDB0266624; DDB_G0273885.
GeneID	8618789. 8619187.
KEGG	ddi:DDB_G0273065. ddi:DDB_G0273885.
Organism-specific databases
dictyBase	DDB_G0273065. dscC-1. DDB_G0273885. dscC-2.
Phylogenomic databases
eggNOG	NOG315723.
OMA	CASILNT.
ProtClustDB	CLSZ2429387.
Family and domain databases
InterPro	IPR000421. Coagulation_fac_5/8-C_type_dom. IPR008979. Galactose-bd-like. IPR019019. H-type_lectin_domain. [Graphical view]
Pfam	PF00754. F5_F8_type_C. 1 hit. PF09458. H_lectin. 1 hit. [Graphical view]
SMART	SM00231. FA58C. 1 hit. [Graphical view]
SUPFAM	SSF49785. Gal_bind_like. 1 hit.
PROSITE	PS01285. FA58C_1. 1 hit. PS01286. FA58C_2. False negative. PS50022. FA58C_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DIS1B_DICDI

Accession

Primary (citable) accession number: P02887
Secondary accession number(s): Q556R2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)