P02883 (THM1_THADA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Thaumatin-1 Alternative name(s): Thaumatin I
Organism	Thaumatococcus daniellii (Katemfe) (Phrynium daniellii)
Taxonomic identifier	4621 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Zingiberales › Marantaceae › Thaumatococcus

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Taste-modifying protein; intensely sweet-tasting. It is 100000 times sweeter than sucrose on a molar basis.
Subcellular location	Cytoplasmic vesicle. Note: Thaumatin accumulates in vesicle-like organelles.
Sequence similarities	Belongs to the thaumatin family.

Ontologies

Keywords
Cellular component	Cytoplasmic vesicle
Molecular function	Taste-modifying protein
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular_component	cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 207

207

Thaumatin-1

PRO_0000096221

Amino acid modifications

Disulfide bond

9 ↔ 204

Ref.2

Disulfide bond

56 ↔ 66

Ref.2

Disulfide bond

71 ↔ 77

Ref.2

Disulfide bond

121 ↔ 193

Ref.2

Disulfide bond

126 ↔ 177

Ref.2

Disulfide bond

134 ↔ 145

Ref.2

Disulfide bond

149 ↔ 158

Ref.2

Disulfide bond

159 ↔ 164

Ref.2

Secondary structure

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207

Helix Strand Turn

Details...

Beta strand

2 – 7

6

Beta strand

9 – 11

3

Beta strand

13 – 18

6

Beta strand

20 – 31

12

Beta strand

36 – 40

5

Beta strand

47 – 55

9

Beta strand

62 – 65

4

Beta strand

67 – 69

3

Beta strand

74 – 76

3

Beta strand

87 – 94

8

Beta strand

97 – 103

7

Beta strand

108 – 110

3

Beta strand

112 – 120

9

Beta strand

124 – 126

3

Helix

130 – 133

4

Helix

136 – 138

3

Beta strand

143 – 145

3

Helix

148 – 152

5

Helix

155 – 158

4

Turn

159 – 161

3

Helix

168 – 176

9

Beta strand

180 – 183

4

Beta strand

191 – 194

4

Beta strand

199 – 204

6

Sequences

	Sequence		Length	Mass (Da)
	P02883 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 9C3288F1873FB32B	FASTA	207	22,204
	10 20 30 40 50 60 ATFEIVNRCS YTVWAAASKG DAALDAGGRQ LNSGESWTIN VEPGTNGGKI WARTDCYFDD 70 80 90 100 110 120 SGSGICKTGD CGGLLRCKRF GRPPTTLAEF SLNQYGKDYI DISNIKGFNV PMNFSPTTRG 130 140 150 160 170 180 CRGVRCAADI VGQCPAKLKA PGGGCNDACT VFQTSEYCCT TGKCGPTEYS RFFKRLCPDA 190 200 FSYVLDKPTT VTCPGSSNYR VTFCPTA

References

[1]	"The complete amino-acid sequence of the sweet protein thaumatin I." Iyengar R.B., Smits P., van der Ouderaa F., van der Wel H., van Brouwershaven J., Ravestein P., Richters G., van Wassenaar P.D. Eur. J. Biochem. 96:193-204(1979) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Three-dimensional structure of thaumatin I, an intensely sweet protein." de Vos A.M., Hatada M., van der Wel H., Krabbendam H., Peerdeman A.F., Kim S.-H. Proc. Natl. Acad. Sci. U.S.A. 82:1406-1409(1985) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
[3]	"X-ray analysis of new crystal forms of the sweet protein thaumatin." McPherson A., Weickmann J. J. Biomol. Struct. Dyn. 7:1053-1060(1990) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[4]	"Crystal structure of a sweet tasting protein thaumatin I, at 1.65-A resolution." Ogata C.M., Gordon P.F., de Vos A.M., Kim S.-H. J. Mol. Biol. 228:893-908(1992) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

QTTC1. A03377.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KWN	X-ray	1.20	A	1-207	[»]
1LR2	X-ray	1.80	A	1-207	[»]
1LR3	X-ray	1.80	A	1-207	[»]
1LXZ	X-ray	1.25	A	1-207	[»]
1LY0	X-ray	1.36	A	1-207	[»]
1PP3	X-ray	1.60	A/B	1-207	[»]
1RQW	X-ray	1.05	A	1-207	[»]
1THI	X-ray	3.20	A	1-207	[»]
1THU	X-ray	2.60	A	1-207	[»]
1THV	X-ray	1.75	A	1-207	[»]
1THW	X-ray	1.75	A	1-207	[»]
2A7I	X-ray	1.75	X	1-207	[»]
2BLR	X-ray	1.40	A	1-206	[»]
2BLU	X-ray	1.40	A	1-206	[»]
2D8O	X-ray	2.38	A	1-207	[»]
2D8P	X-ray	2.30	A	1-207	[»]
2G4Y	X-ray	1.98	A	1-207	[»]
2OQN	X-ray	1.90	A	1-207	[»]
2PE7	X-ray	1.46	A	1-207	[»]
2VHK	X-ray	0.94	A	1-206	[»]
2VHR	X-ray	0.95	A	1-207	[»]
2VI1	X-ray	1.04	A	1-207	[»]
2VI2	X-ray	1.08	A	1-207	[»]
2VI3	X-ray	0.98	A	1-207	[»]
2VI4	X-ray	1.10	A	1-207	[»]
2VU6	X-ray	0.95	A	1-207	[»]
2VU7	X-ray	1.08	A	1-207	[»]
2WBZ	X-ray	1.60	A	1-206	[»]
3AL7	X-ray	1.10	A	1-207	[»]
3ALD	X-ray	1.10	A	1-207	[»]
3DZN	X-ray	1.51	A	1-207	[»]
3DZP	X-ray	1.51	A	1-207	[»]
3DZR	X-ray	1.51	A	1-207	[»]
3E0A	X-ray	1.51	A	1-207	[»]
3E3S	X-ray	1.73	A	1-207	[»]
3N02	X-ray	1.50	A	1-206	[»]
3N03	X-ray	1.50	A	1-206	[»]
3QY5	X-ray	1.25	A	1-207	[»]
3V7V	X-ray	2.30	A	1-207	[»]
3V82	X-ray	2.30	A	1-207	[»]
3V84	X-ray	2.30	A	1-207	[»]
3V87	X-ray	2.30	A	1-207	[»]
3V88	X-ray	2.30	A	1-207	[»]
3V8A	X-ray	2.30	A	1-207	[»]
3VCE	X-ray	2.30	A	1-207	[»]
3VCG	X-ray	2.30	A	1-207	[»]
3VCH	X-ray	2.30	A	1-207	[»]
3VCI	X-ray	2.30	A	1-207	[»]
3VCJ	X-ray	2.30	A	1-207	[»]
3VCK	X-ray	2.30	A	1-207	[»]
3VHF	X-ray	1.39	A	1-207	[»]
3VHG	X-ray	1.00	A	1-207	[»]
3VJQ	X-ray	1.00	A	1-207	[»]
3ZEJ	X-ray	1.55	A	1-207	[»]
4AXR	X-ray	1.38	A	1-207	[»]
4AXU	X-ray	1.38	A	1-206	[»]
4BAL	X-ray	1.30	A	1-207	[»]
4BAR	X-ray	1.20	A	1-207	[»]
4DC5	X-ray	1.48	A	1-207	[»]
4DC6	X-ray	1.48	A	1-207	[»]
4DIY	X-ray	1.98	A	1-207	[»]
4DIZ	X-ray	1.98	A	1-207	[»]
4DJ0	X-ray	1.98	A	1-207	[»]
4DJ1	X-ray	1.98	A	1-207	[»]
4EK0	X-ray	1.52	A	1-207	[»]
4EKA	X-ray	1.55	A	1-207	[»]
4EKB	X-ray	1.52	A	1-207	[»]
4EKH	X-ray	1.75	A	1-207	[»]
4EKO	X-ray	1.52	A	1-207	[»]
4EKT	X-ray	1.75	A	1-207	[»]
4EL2	X-ray	1.52	A	1-207	[»]
4EL3	X-ray	1.95	A	1-207	[»]
4EL7	X-ray	1.52	A	1-207	[»]
4ELA	X-ray	2.00	A	1-207	[»]

ProteinModelPortal

P02883.

SMR

P02883. Positions 1-207.

ModBase

Search...

Protein family/group databases

Allergome

9233. Tha da TLP.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.60.110.10. 1 hit.

InterPro

IPR001938. Thaumatin.
IPR017949. Thaumatin_CS.
[Graphical view]

Pfam

PF00314. Thaumatin. 1 hit.
[Graphical view]

PIRSF

PIRSF002703. Thaumatin. 1 hit.

PRINTS

PR00347. THAUMATIN.

SMART

SM00205. THN. 1 hit.
[Graphical view]

SUPFAM

SSF49870. Thaumatin. 1 hit.

PROSITE

PS00316. THAUMATIN_1. 1 hit.
PS51367. THAUMATIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P02883.

Entry information

Entry name

THM1_THADA

Accession

Primary (citable) accession number: P02883

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program