ID MONB_DIOCU Reviewed; 50 AA. AC P02882; DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot. DT 01-AUG-1991, sequence version 2. DT 24-JAN-2024, entry version 120. DE RecName: Full=Monellin chain B; DE AltName: Full=Monellin chain II; OS Dioscoreophyllum cumminsii (Serendipity berry) (Rhopalandria cumminsii). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Ranunculales; Menispermaceae; OC Chasmantheroideae; Burasaieae; Dioscoreophyllum. OX NCBI_TaxID=3457; RN [1] RP PROTEIN SEQUENCE. RX PubMed=1368575; DOI=10.1271/bbb1961.54.2219; RA Kohmura M., Nio N., Ariyoshi Y.; RT "Complete amino acid sequence of the sweet protein monellin."; RL Agric. Biol. Chem. 54:2219-2224(1990). RN [2] RP PROTEIN SEQUENCE. RX PubMed=964918; DOI=10.1515/bchm2.1976.357.1.585; RA Frank G., Zuber H.; RT "The complete amino acid sequences of both subunits of the sweet protein RT monellin."; RL Hoppe-Seyler's Z. Physiol. Chem. 357:585-592(1976). RN [3] RP PROTEIN SEQUENCE. RX PubMed=4107; DOI=10.1016/0005-2795(76)90293-2; RA Bohak Z., Li S.-L.; RT "The structure of monellin and its relation to the sweetness of the RT protein."; RL Biochim. Biophys. Acta 427:153-170(1976). RN [4] RP X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS). RX PubMed=3614382; DOI=10.1038/328739a0; RA Ogata C., Hatada M., Tomlinson G., Shin W.-C., Kim S.-H.; RT "Crystal structure of the intensely sweet protein monellin."; RL Nature 328:739-742(1987). RN [5] RP X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS). RX PubMed=8230222; DOI=10.1006/jmbi.1993.1594; RA Somoza J.R., Jiang F., Tong L., Kang C.-H., Cho J.M., Kim S.-H.; RT "Two crystal structures of a potently sweet protein. Natural monellin at RT 2.75-A resolution and single-chain monellin at 1.7-A resolution."; RL J. Mol. Biol. 234:390-404(1993). RN [6] RP X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS). RX PubMed=15299859; DOI=10.1107/s0907444997006860; RA Bujacz G., Miller M., Harrison R., Thanki N., Gilliland G.L., Ogata C., RA Kim S.-H., Wlodawer A.; RT "Structure of monellin refined to 2.3-A resolution in the orthorhombic RT crystal form."; RL Acta Crystallogr. D 53:713-719(1997). RN [7] RP SIMILARITY TO CYSTATINS. RX PubMed=8464079; DOI=10.1006/jmbi.1993.1186; RA Murzin A.G.; RT "Sweet-tasting protein monellin is related to the cystatin family of thiol RT proteinase inhibitors."; RL J. Mol. Biol. 230:689-694(1993). CC -!- FUNCTION: Taste-modifying protein; intensely sweet-tasting protein. CC -!- SUBUNIT: Heterodimer of an A chain and a B chain. CC -!- BIOTECHNOLOGY: Natural monellin has not been mass marketed to the food CC industry, primarily due to the challenge of large-scale purification of CC the protein which is relatively sensitive to heat or acid treatment. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR PIR; JH0210; MLDIB. DR PDB; 1FA3; NMR; -; A=1-48. DR PDB; 1FUW; NMR; -; A=1-48. DR PDB; 1IV7; X-ray; 1.82 A; A/B=1-48. DR PDB; 1IV9; X-ray; 1.90 A; A/B=1-50. DR PDB; 1KRL; X-ray; 1.90 A; B/D=1-50. DR PDB; 1M9G; NMR; -; A=1-48. DR PDB; 1MNL; NMR; -; A=1-48. DR PDB; 1MOL; X-ray; 1.70 A; A/B=1-48. DR PDB; 2O9U; X-ray; 1.15 A; X=1-48. DR PDB; 3MON; X-ray; 2.80 A; B/D/F/H=1-48. DR PDB; 3PXM; X-ray; 1.80 A; A/B=1-48. DR PDB; 3PYJ; X-ray; 2.00 A; A=1-48. DR PDB; 3Q2P; X-ray; 2.34 A; A/B/C/D=1-48. DR PDB; 4MON; X-ray; 2.30 A; B/D=1-48. DR PDB; 5LC6; X-ray; 1.70 A; A/B=1-48. DR PDB; 5LC7; X-ray; 1.55 A; A/B=1-48. DR PDB; 5O7K; X-ray; 2.05 A; A/B=1-48. DR PDB; 5O7L; X-ray; 2.60 A; A/B=1-48. DR PDB; 5O7Q; X-ray; 1.72 A; A/B=1-48. DR PDB; 5O7R; X-ray; 2.06 A; A/B=1-48. DR PDB; 5O7S; X-ray; 2.02 A; A/B=1-48. DR PDB; 5XFU; X-ray; 2.61 A; A/B/C/D/E=1-48. DR PDB; 5YCT; X-ray; 1.85 A; A/B=1-48. DR PDB; 5YCU; X-ray; 2.32 A; A/B/C/D/E=1-46. DR PDB; 5YCW; X-ray; 2.29 A; A=1-46. DR PDB; 5Z1P; X-ray; 1.89 A; A/B/C/D=1-48. DR PDB; 6L44; X-ray; 2.49 A; A/B=1-46. DR PDB; 6L4I; X-ray; 2.20 A; A/B=1-48. DR PDB; 6L4J; X-ray; 2.30 A; A/B=1-48. DR PDB; 6L4N; X-ray; 2.43 A; A/B=1-46. DR PDB; 6LAY; X-ray; 3.00 A; A/B=1-48. DR PDB; 7D75; X-ray; 2.50 A; A/B/C/D=1-49. DR PDB; 7EUA; X-ray; 2.43 A; A=1-48. DR PDB; 7VWW; X-ray; 2.70 A; A/B/C/D/E/F/G/H=1-48. DR PDB; 8JZ0; X-ray; 1.23 A; A/B=1-48. DR PDB; 8JZ1; X-ray; 1.24 A; A/B=1-48. DR PDBsum; 1FA3; -. DR PDBsum; 1FUW; -. DR PDBsum; 1IV7; -. DR PDBsum; 1IV9; -. DR PDBsum; 1KRL; -. DR PDBsum; 1M9G; -. DR PDBsum; 1MNL; -. DR PDBsum; 1MOL; -. DR PDBsum; 2O9U; -. DR PDBsum; 3MON; -. DR PDBsum; 3PXM; -. DR PDBsum; 3PYJ; -. DR PDBsum; 3Q2P; -. DR PDBsum; 4MON; -. DR PDBsum; 5LC6; -. DR PDBsum; 5LC7; -. DR PDBsum; 5O7K; -. DR PDBsum; 5O7L; -. DR PDBsum; 5O7Q; -. DR PDBsum; 5O7R; -. DR PDBsum; 5O7S; -. DR PDBsum; 5XFU; -. DR PDBsum; 5YCT; -. DR PDBsum; 5YCU; -. DR PDBsum; 5YCW; -. DR PDBsum; 5Z1P; -. DR PDBsum; 6L44; -. DR PDBsum; 6L4I; -. DR PDBsum; 6L4J; -. DR PDBsum; 6L4N; -. DR PDBsum; 6LAY; -. DR PDBsum; 7D75; -. DR PDBsum; 7EUA; -. DR PDBsum; 7VWW; -. DR PDBsum; 8JZ0; -. DR PDBsum; 8JZ1; -. DR AlphaFoldDB; P02882; -. DR BMRB; P02882; -. DR PCDDB; P02882; -. DR SMR; P02882; -. DR IntAct; P02882; 1. DR MINT; P02882; -. DR EvolutionaryTrace; P02882; -. DR Gene3D; 6.10.140.2000; -; 1. DR InterPro; IPR046350; Cystatin_sf. DR InterPro; IPR015283; Monellin. DR InterPro; IPR002095; Monellin_B. DR Pfam; PF09200; Monellin; 1. DR PRINTS; PR00631; MONELLINB. DR SUPFAM; SSF54403; Cystatin/monellin; 1. PE 1: Evidence at protein level; KW 3D-structure; Direct protein sequencing; Taste-modifying protein. FT CHAIN 1..50 FT /note="Monellin chain B" FT /id="PRO_0000207166" FT SITE 41 FT /note="Blocking, abolishes the sweet taste" FT CONFLICT 49..50 FT /note="EN -> NE (in Ref. 2; AA sequence)" FT /evidence="ECO:0000305" FT STRAND 4..6 FT /evidence="ECO:0007829|PDB:2O9U" FT HELIX 10..26 FT /evidence="ECO:0007829|PDB:2O9U" FT TURN 27..29 FT /evidence="ECO:0007829|PDB:5LC7" FT STRAND 34..47 FT /evidence="ECO:0007829|PDB:2O9U" SQ SEQUENCE 50 AA; 5835 MW; F08396DFCD480C81 CRC64; GEWEIIDIGP FTQNLGKFAV DEENKIGQYG RLTFNKVIRP CMKKTIYEEN //