P02881 (MONA_DIOCU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Monellin chain A Alternative name(s): Monellin chain I
Organism	Dioscoreophyllum cumminsii (Serendipity berry)
Taxonomic identifier	3457 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › Ranunculales › Menispermaceae › Dioscoreophyllum

Protein attributes

Sequence length	45 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Taste-modifying protein; intensely sweet-tasting protein.
Subunit structure	Heterodimer of an A chain and a B chain.
Biotechnological use	Natural monellin has not been mass marketed to the food industry, primarily due to the challenge of large-scale purification of the protein which is relatively sensitive to heat or acid treatment.

Ontologies

Keywords
Molecular function	Taste-modifying protein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 45

45

Monellin chain A

PRO_0000207165

Natural variations

Natural variant

1

1

Missing in 90% of the chains.

Experimental info

Sequence conflict

23

1

D → N AA sequence Ref.3

Sequence conflict

26 – 27

2

ED → QN AA sequence Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

45

Helix Strand Turn

Details...

Beta strand

4 – 15

12

Beta strand

18 – 27

10

Turn

28 – 30

3

Beta strand

33 – 39

7

Sequences

	Sequence		Length	Mass (Da)
	P02881 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: E086DF18521EC5B6	FASTA	45	5,398
	10 20 30 40 FREIKGYEYQ LYVYASDKLF RADISEDYKT RGRKLLRFNG PVPPP

References

[1]	"Complete amino acid sequence of the sweet protein monellin." Kohmura M., Nio N., Ariyoshi Y. Agric. Biol. Chem. 54:2219-2224(1990) [PubMed: 1368575] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"The complete amino acid sequences of both subunits of the sweet protein monellin." Frank G., Zuber H. Hoppe-Seyler's Z. Physiol. Chem. 357:585-592(1976) [PubMed: 964918] [Abstract] Cited for: PROTEIN SEQUENCE.
[3]	"Mass spectrometric sequencing of proteins. The structure of subunit I of monellin." Hudson G., Biemann K. Biochem. Biophys. Res. Commun. 71:212-220(1976) [PubMed: 962914] [Abstract] Cited for: PROTEIN SEQUENCE.
[4]	"The structure of monellin and its relation to the sweetness of the protein." Bohak Z., Li S.-L. Biochim. Biophys. Acta 427:153-170(1976) [PubMed: 4107] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-28.
[5]	"Crystal structure of the intensely sweet protein monellin." Ogata C., Hatada M., Tomlinson G., Shin W.-C., Kim S.-H. Nature 328:739-742(1987) [PubMed: 3614382] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
[6]	"Structure of monellin refined to 2.3-A resolution in the orthorhombic crystal form." Bujacz G., Miller M., Harrison R., Thanki N., Gilliland G.L., Ogata C., Kim S.-H., Wlodawer A. Acta Crystallogr. D 53:713-719(1997) [PubMed: 15299859] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[7]	"Sweet-tasting protein monellin is related to the cystatin family of thiol proteinase inhibitors." Murzin A.G. J. Mol. Biol. 230:689-694(1993) [PubMed: 8464079] [Abstract] Cited for: SIMILARITY TO CYSTATINS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

MLDIA. JH0209.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FA3	NMR	-	A	1-45	[»]
1FUW	NMR	-	A	2-42	[»]
1IV7	X-ray	1.82	A/B	1-45	[»]
1IV9	X-ray	1.90	A/B	1-45	[»]
1KRL	X-ray	1.90	A/C	2-45	[»]
1M9G	NMR	-	A	1-45	[»]
1MNL	NMR	-	A	2-45	[»]
2GPK	model	-	B	2-45	[»]
3MON	X-ray	2.80	A/C/E/G	2-45	[»]
3PXM	X-ray	1.80	A/B	1-45	[»]
3PYJ	X-ray	2.00	A	1-45	[»]
3Q2P	X-ray	2.34	A/B/C/D	1-45	[»]
4MON	X-ray	2.30	A/C	1-45	[»]

ProteinModelPortal

P02881.

SMR

P02881. Positions 1-45.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR015283. Monellin.
IPR000828. Monellin_A.
[Graphical view]

Pfam

PF09200. Monellin. 1 hit.
[Graphical view]

PRINTS

PR00630. MONELLINA.

ProtoNet

Search...

Entry information

Entry name

MONA_DIOCU

Accession

Primary (citable) accession number: P02881

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	June 28, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program