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P02881 (MONA_DIOCU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Monellin chain A
Alternative name(s):
Monellin chain I
OrganismDioscoreophyllum cumminsii (Serendipity berry)
Taxonomic identifier3457 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsRanunculalesMenispermaceaeTinosporoideaeDioscoreophyllum

Protein attributes

Sequence length45 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Taste-modifying protein; intensely sweet-tasting protein.

Subunit structure

Heterodimer of an A chain and a B chain.

Biotechnological use

Natural monellin has not been mass marketed to the food industry, primarily due to the challenge of large-scale purification of the protein which is relatively sensitive to heat or acid treatment.

Ontologies

Keywords
   Molecular functionTaste-modifying protein
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4545Monellin chain A
PRO_0000207165

Natural variations

Natural variant11Missing in 90% of the chains.

Experimental info

Sequence conflict231D → N AA sequence Ref.3
Sequence conflict26 – 272ED → QN AA sequence Ref.3

Secondary structure

........ 45
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P02881 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: E086DF18521EC5B6

FASTA455,398
        10         20         30         40 
FREIKGYEYQ LYVYASDKLF RADISEDYKT RGRKLLRFNG PVPPP 

« Hide

References

[1]"Complete amino acid sequence of the sweet protein monellin."
Kohmura M., Nio N., Ariyoshi Y.
Agric. Biol. Chem. 54:2219-2224(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
[2]"The complete amino acid sequences of both subunits of the sweet protein monellin."
Frank G., Zuber H.
Hoppe-Seyler's Z. Physiol. Chem. 357:585-592(1976) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
[3]"Mass spectrometric sequencing of proteins. The structure of subunit I of monellin."
Hudson G., Biemann K.
Biochem. Biophys. Res. Commun. 71:212-220(1976) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
[4]"The structure of monellin and its relation to the sweetness of the protein."
Bohak Z., Li S.-L.
Biochim. Biophys. Acta 427:153-170(1976) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-28.
[5]"Crystal structure of the intensely sweet protein monellin."
Ogata C., Hatada M., Tomlinson G., Shin W.-C., Kim S.-H.
Nature 328:739-742(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
[6]"Structure of monellin refined to 2.3-A resolution in the orthorhombic crystal form."
Bujacz G., Miller M., Harrison R., Thanki N., Gilliland G.L., Ogata C., Kim S.-H., Wlodawer A.
Acta Crystallogr. D 53:713-719(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[7]"Sweet-tasting protein monellin is related to the cystatin family of thiol proteinase inhibitors."
Murzin A.G.
J. Mol. Biol. 230:689-694(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: SIMILARITY TO CYSTATINS.
+Additional computationally mapped references.

Cross-references

Sequence databases

PIRMLDIA. JH0209.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1FA3NMR-A1-45[»]
1FUWNMR-A2-42[»]
1IV7X-ray1.82A/B1-45[»]
1IV9X-ray1.90A/B1-45[»]
1KRLX-ray1.90A/C2-45[»]
1M9GNMR-A1-45[»]
1MNLNMR-A2-45[»]
2GPKmodel-B2-45[»]
3MONX-ray2.80A/C/E/G2-45[»]
3PXMX-ray1.80A/B1-45[»]
3PYJX-ray2.00A1-45[»]
3Q2PX-ray2.34A/B/C/D1-45[»]
4MONX-ray2.30A/C1-45[»]
ProteinModelPortalP02881.
SMRP02881. Positions 1-45.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1542282.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR015283. Monellin.
IPR000828. Monellin_A.
[Graphical view]
PfamPF09200. Monellin. 1 hit.
[Graphical view]
PRINTSPR00630. MONELLINA.
ProtoNetSearch...

Other

EvolutionaryTraceP02881.

Entry information

Entry nameMONA_DIOCU
AccessionPrimary (citable) accession number: P02881
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references