Reviewed, UniProtKB/Swiss-Prot P02877 (HEVE_HEVBR)

Names and origin

Protein names

Recommended name:
    Pro-hevein
Alternative name(s):
    Major hevein
Cleaved into the following 2 chains:
    1- Recommended name:
            Hevein
        Alternative name(s):
            Allergen=Hev b 6
    2- Recommended name:
            Win-like protein

Gene names

Name:

HEV1

Organism

Hevea brasiliensis (Para rubber tree)

Taxonomic identifier

3981 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Malpighiales › Euphorbiaceae › Crotonoideae › Micrandreae › Hevea

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin. Can inhibit fungal growth.
Tissue specificity	Laticifer.
Post-translational modification	Proteolytically processed to yield the two chains of the mature protein.
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Contains 1 barwin domain. Contains 1 chitin-binding type-1 domain.

Ontologies

Keywords
Disease	Allergen
Domain	Signal
Ligand	Chitin-binding
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: InterPro defense response to bacterium Inferred from electronic annotation. Source: InterPro defense response to fungus Inferred from electronic annotation. Source: InterPro
Molecular function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 17

17

Ref.2 Ref.4 Ref.5

Chain

18 – 204

187

Pro-hevein

PRO_0000005280

Chain

18 – 60

43

Hevein

PRO_0000005281

Propeptide

61 – 66

6

PRO_0000005282

Chain

67 – 204

138

Win-like protein

PRO_0000005283

Regions

Domain

18 – 60

43

Chitin-binding type-1

Domain

68 – 189

122

Barwin

Amino acid modifications

Disulfide bond

20 ↔ 35

Ref.7

Disulfide bond

29 ↔ 41

Ref.7

Disulfide bond

34 ↔ 48

Ref.7

Disulfide bond

54 ↔ 58

Ref.7

Disulfide bond

96 ↔ 128

By similarity

Disulfide bond

117 ↔ 151

By similarity

Disulfide bond

131 ↔ 187

By similarity

Natural variations

Natural variant

31

1

N → D Possible deamidation.

Natural variant

73

1

V → M

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

204

Helix Strand Turn

Details...

Helix

21 – 24

4

Helix

30 – 32

3

Beta strand

41 – 44

4

Helix

45 – 48

4

Helix

50 – 52

3

Sequences

	Sequence		Length	Mass (Da)
	P02877-1 [UniParc]. Last modified November 1, 1990. Version 2. Checksum: FBCEE57CDFC80569	FASTA	204	21,859
	10 20 30 40 50 60 MNIFIVVLLC LTGVAIAEQC GRQAGGKLCP NNLCCSQWGW CGSTDEYCSP DHNCQSNCKD 70 80 90 100 110 120 SGEGVGGGSA SNVLATYHLY NSQDHGWDLN AASAYCSTWD ANKPYSWRSK YGWTAFCGPV 130 140 150 160 170 180 GAHGQSSCGK CLSVTNTGTG AKTTVRIVDQ CSNGGLDLDV NVFRQLDTDG KGYERGHITV 190 200 NYQFVDCGDS FNPLFSVMKS SVIN

References

[1]	"Wound-induced accumulation of mRNA containing a hevein sequence in laticifers of rubber tree (Hevea brasiliensis)." Broekaert W.F., Lee H.I., Kush A., Chua N.H., Raikhel N. Proc. Natl. Acad. Sci. U.S.A. 87:7633-7637(1990) [PubMed: 2217194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Amino-acid sequence of hevein." Walujono K., Scholma R.A., Beintema J.J., Mariono A., Hahn A.M. (In) Proceedings of the international rubber conference, pp.2:518-531, Rubber Research Institute of Malaysia, Kuala Lumpur (1975) Cited for: PROTEIN SEQUENCE OF 18-60. Tissue: Latex.
[3]	Beintema J.J. Submitted (JUN-1977) to the PIR data bank Cited for: SEQUENCE REVISION TO 51-52.
[4]	"Co- and post-translational processing of the hevein preproprotein of latex of the rubber tree (Hevea brasiliensis)." Lee H.-I., Broekaert W.F., Raikhel N.V. J. Biol. Chem. 266:15944-15948(1991) [PubMed: 1874741] [Abstract] Cited for: PROTEIN SEQUENCE OF 18-26 AND 67-77, PROTEOLYTIC PROCESSING. Tissue: Latex.
[5]	"Characterization of latex allergenic components by capillary zone electrophoresis and N-terminal sequence analysis." Chen H.-D., Chen C.-L., Huang S.-W., Kung H.-F., Chen H.-C. J. Biomed. Sci. 5:421-427(1998) [PubMed: 9845845] [Abstract] Cited for: PROTEIN SEQUENCE OF 18-35. Tissue: Latex.
[6]	"Crystal structure of hevein at 2.8-A resolution." Rodriguez-Romero A., Ravichandran K.G., Soriano-Garcia M. FEBS Lett. 291:307-309(1991) [PubMed: 1936279] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 18-60.
[7]	"Hevein: NMR assignment and assessment of solution-state folding for the agglutinin-toxin motif." Andersen N.H., Cao B., Rodriguez-Romero A., Arreguin B. Biochemistry 32:1407-1422(1993) [PubMed: 8431421] [Abstract] Cited for: STRUCTURE BY NMR OF 18-60, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M36986 mRNA. Translation: AAA33357.1.

PIR

HVHV. A38288.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HEV	NMR	-	A	18-60	[»]
1Q9B	X-ray	1.50	A	18-60	[»]
1T0W	NMR	-	A	18-49	[»]
1WKX	X-ray	1.70	A	18-60	[»]

ModBase

Search...

Protein family/group databases

CAZy

CBM18. Carbohydrate-Binding Module Family 18.

Family and domain databases

InterPro

IPR001153. Barwin.
IPR014733. Barwin-like_endoglucanase.
IPR018226. Barwin_CS.
IPR018371. Chitin-binding_1_CS.
IPR001002. Chitin_bd_1.
IPR000726. Glyco_hydro_19_cat.
[Graphical view]

Gene3D

G3DSA:2.40.40.10. Barwin. 1 hit.
G3DSA:3.30.60.10. Chitin_bd_1. 1 hit.

PANTHER

PTHR22595:SF1. Barwin. 1 hit.
PTHR22595. Glyco_hydro_19_cat. 1 hit.

Pfam

PF00967. Barwin. 1 hit.
PF00187. Chitin_bind_1. 1 hit.
[Graphical view]

PRINTS

PR00602. BARWIN.
PR00451. CHITINBINDNG.

ProDom

PD004535. Barwin. 1 hit.
PD000609. Chitin_binding_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00270. ChtBD1. 1 hit.
[Graphical view]

PROSITE

PS00771. BARWIN_1. 1 hit.
PS00772. BARWIN_2. 1 hit.
PS51174. BARWIN_3. 1 hit.
PS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

HEVE_HEVBR

Accession

Primary (citable) accession number: P02877

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	November 1, 1990
Last modified:	June 16, 2009
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)