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P02877

- HEVE_HEVBR

UniProt

P02877 - HEVE_HEVBR

Protein

Pro-hevein

Gene

HEV1

Organism
Hevea brasiliensis (Para rubber tree) (Siphonia brasiliensis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 2 (01 Nov 1990)
      Previous versions | rss
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    Functioni

    N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin. Can inhibit fungal growth.

    GO - Molecular functioni

    1. chitin binding Source: UniProtKB-KW

    GO - Biological processi

    1. defense response to bacterium Source: InterPro
    2. defense response to fungus Source: InterPro

    Keywords - Ligandi

    Chitin-binding

    Protein family/group databases

    CAZyiCBM18. Carbohydrate-Binding Module Family 18.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pro-hevein
    Alternative name(s):
    Major hevein
    Cleaved into the following 2 chains:
    Alternative name(s):
    Allergen: Hev b 6
    Gene namesi
    Name:HEV1
    OrganismiHevea brasiliensis (Para rubber tree) (Siphonia brasiliensis)
    Taxonomic identifieri3981 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesEuphorbiaceaeCrotonoideaeMicrandreaeHevea

    Pathology & Biotechi

    Allergenic propertiesi

    Causes an allergic reaction in human.

    Keywords - Diseasei

    Allergen

    Protein family/group databases

    Allergomei390. Hev b 6.
    391. Hev b 6.01.
    392. Hev b 6.02.
    393. Hev b 6.03.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 17173 PublicationsAdd
    BLAST
    Chaini18 – 204187Pro-heveinPRO_0000005280Add
    BLAST
    Chaini18 – 6043HeveinPRO_0000005281Add
    BLAST
    Propeptidei61 – 6661 PublicationPRO_0000005282
    Chaini67 – 204138Win-like proteinPRO_0000005283Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi20 ↔ 351 PublicationPROSITE-ProRule annotation
    Disulfide bondi29 ↔ 411 PublicationPROSITE-ProRule annotation
    Disulfide bondi34 ↔ 481 PublicationPROSITE-ProRule annotation
    Disulfide bondi54 ↔ 581 PublicationPROSITE-ProRule annotation
    Disulfide bondi96 ↔ 128PROSITE-ProRule annotation
    Disulfide bondi117 ↔ 151PROSITE-ProRule annotation
    Disulfide bondi131 ↔ 187PROSITE-ProRule annotation

    Post-translational modificationi

    Proteolytically processed to yield the two chains of the mature protein.1 Publication

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Tissue specificityi

    Laticifer.

    Structurei

    Secondary structure

    1
    204
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi21 – 244
    Helixi30 – 323
    Beta strandi37 – 393
    Beta strandi41 – 444
    Helixi45 – 484
    Helixi50 – 523

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1HEVNMR-A18-60[»]
    1Q9BX-ray1.50A18-60[»]
    1T0WNMR-A18-49[»]
    1WKXX-ray1.70A18-60[»]
    ProteinModelPortaliP02877.
    SMRiP02877. Positions 18-60, 70-189.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP02877.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini18 – 6043Chitin-binding type-1PROSITE-ProRule annotationAdd
    BLAST
    Domaini68 – 189122BarwinPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 barwin domain.PROSITE-ProRule annotation
    Contains 1 chitin-binding type-1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.40.40.10. 1 hit.
    3.30.60.10. 1 hit.
    InterProiIPR001153. Barwin.
    IPR014733. Barwin-like_endoglucanase.
    IPR018226. Barwin_CS.
    IPR001002. Chitin-bd_1.
    IPR018371. Chitin-binding_1_CS.
    IPR009009. RlpA-like_DPBB.
    [Graphical view]
    PfamiPF00967. Barwin. 1 hit.
    PF00187. Chitin_bind_1. 1 hit.
    [Graphical view]
    PRINTSiPR00602. BARWIN.
    PR00451. CHITINBINDNG.
    ProDomiPD004535. Barwin. 1 hit.
    PD000609. Chitin_bd_1. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00270. ChtBD1. 1 hit.
    [Graphical view]
    SUPFAMiSSF50685. SSF50685. 1 hit.
    SSF57016. SSF57016. 1 hit.
    PROSITEiPS00771. BARWIN_1. 1 hit.
    PS00772. BARWIN_2. 1 hit.
    PS51174. BARWIN_3. 1 hit.
    PS00026. CHIT_BIND_I_1. 1 hit.
    PS50941. CHIT_BIND_I_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P02877-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNIFIVVLLC LTGVAIAEQC GRQAGGKLCP NNLCCSQWGW CGSTDEYCSP    50
    DHNCQSNCKD SGEGVGGGSA SNVLATYHLY NSQDHGWDLN AASAYCSTWD 100
    ANKPYSWRSK YGWTAFCGPV GAHGQSSCGK CLSVTNTGTG AKTTVRIVDQ 150
    CSNGGLDLDV NVFRQLDTDG KGYERGHITV NYQFVDCGDS FNPLFSVMKS 200
    SVIN 204
    Length:204
    Mass (Da):21,859
    Last modified:November 1, 1990 - v2
    Checksum:iFBCEE57CDFC80569
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti31 – 311N → D Possible deamidation.
    Natural varianti73 – 731V → M.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36986 mRNA. Translation: AAA33357.1.
    PIRiA38288. HVHV.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36986 mRNA. Translation: AAA33357.1 .
    PIRi A38288. HVHV.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1HEV NMR - A 18-60 [» ]
    1Q9B X-ray 1.50 A 18-60 [» ]
    1T0W NMR - A 18-49 [» ]
    1WKX X-ray 1.70 A 18-60 [» ]
    ProteinModelPortali P02877.
    SMRi P02877. Positions 18-60, 70-189.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 390. Hev b 6.
    391. Hev b 6.01.
    392. Hev b 6.02.
    393. Hev b 6.03.
    CAZyi CBM18. Carbohydrate-Binding Module Family 18.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P02877.

    Family and domain databases

    Gene3Di 2.40.40.10. 1 hit.
    3.30.60.10. 1 hit.
    InterProi IPR001153. Barwin.
    IPR014733. Barwin-like_endoglucanase.
    IPR018226. Barwin_CS.
    IPR001002. Chitin-bd_1.
    IPR018371. Chitin-binding_1_CS.
    IPR009009. RlpA-like_DPBB.
    [Graphical view ]
    Pfami PF00967. Barwin. 1 hit.
    PF00187. Chitin_bind_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00602. BARWIN.
    PR00451. CHITINBINDNG.
    ProDomi PD004535. Barwin. 1 hit.
    PD000609. Chitin_bd_1. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00270. ChtBD1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50685. SSF50685. 1 hit.
    SSF57016. SSF57016. 1 hit.
    PROSITEi PS00771. BARWIN_1. 1 hit.
    PS00772. BARWIN_2. 1 hit.
    PS51174. BARWIN_3. 1 hit.
    PS00026. CHIT_BIND_I_1. 1 hit.
    PS50941. CHIT_BIND_I_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Wound-induced accumulation of mRNA containing a hevein sequence in laticifers of rubber tree (Hevea brasiliensis)."
      Broekaert W.F., Lee H.I., Kush A., Chua N.H., Raikhel N.
      Proc. Natl. Acad. Sci. U.S.A. 87:7633-7637(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Amino-acid sequence of hevein."
      Walujono K., Scholma R.A., Beintema J.J., Mariono A., Hahn A.M.
      (In) Proceedings of the international rubber conference, pp.2:518-531, Rubber Research Institute of Malaysia, Kuala Lumpur (1975)
      Cited for: PROTEIN SEQUENCE OF 18-60.
      Tissue: Latex.
    3. Beintema J.J.
      Submitted (JUN-1977) to the PIR data bank
      Cited for: SEQUENCE REVISION TO 51-52.
    4. "Co- and post-translational processing of the hevein preproprotein of latex of the rubber tree (Hevea brasiliensis)."
      Lee H.-I., Broekaert W.F., Raikhel N.V.
      J. Biol. Chem. 266:15944-15948(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 18-26 AND 67-77, PROTEOLYTIC PROCESSING.
      Tissue: Latex.
    5. "Characterization of latex allergenic components by capillary zone electrophoresis and N-terminal sequence analysis."
      Chen H.-D., Chen C.-L., Huang S.-W., Kung H.-F., Chen H.-C.
      J. Biomed. Sci. 5:421-427(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 18-35.
      Tissue: Latex.
    6. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 18-60.
    7. "Hevein: NMR assignment and assessment of solution-state folding for the agglutinin-toxin motif."
      Andersen N.H., Cao B., Rodriguez-Romero A., Arreguin B.
      Biochemistry 32:1407-1422(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 18-60, DISULFIDE BONDS.

    Entry informationi

    Entry nameiHEVE_HEVBR
    AccessioniPrimary (citable) accession number: P02877
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. Allergens
      Nomenclature of allergens and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3