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P02876

- AGI2_WHEAT

UniProt

P02876 - AGI2_WHEAT

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Protein
Agglutinin isolectin 2
Gene
N/A
Organism
Triticum aestivum (Wheat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin.

GO - Molecular functioni

  1. chitin binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

Chitin-binding, Lectin

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Agglutinin isolectin 2
Alternative name(s):
Isolectin D
WGA2
OrganismiTriticum aestivum (Wheat)
Taxonomic identifieri4565 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeTriticum
ProteomesiUP000019116: Unplaced

Organism-specific databases

GrameneiP02876.

Pathology & Biotechi

Protein family/group databases

Allergomei650. Tri a 18.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 27271 Publication
Add
BLAST
Chaini28 – 198171Agglutinin isolectin 2
PRO_0000005257Add
BLAST
Propeptidei199 – 21315
PRO_0000005258Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei28 – 281Pyrrolidone carboxylic acid1 Publication
Disulfide bondi30 ↔ 451 Publication
Disulfide bondi39 ↔ 511 Publication
Disulfide bondi44 ↔ 581 Publication
Disulfide bondi62 ↔ 671 Publication
Disulfide bondi73 ↔ 881 Publication
Disulfide bondi82 ↔ 941 Publication
Disulfide bondi87 ↔ 1011 Publication
Disulfide bondi105 ↔ 1101 Publication
Disulfide bondi116 ↔ 1311 Publication
Disulfide bondi125 ↔ 1371 Publication
Disulfide bondi130 ↔ 1441 Publication
Disulfide bondi148 ↔ 1531 Publication
Disulfide bondi159 ↔ 1741 Publication
Disulfide bondi168 ↔ 1801 Publication
Disulfide bondi173 ↔ 1871 Publication
Disulfide bondi191 ↔ 1961 Publication

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

Homodimer, u-shaped.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi31 – 344
Helixi40 – 423
Helixi55 – 584
Beta strandi64 – 663
Helixi74 – 774
Helixi83 – 853
Beta strandi92 – 954
Helixi98 – 1014
Beta strandi107 – 1093
Helixi118 – 1203
Helixi126 – 1283
Beta strandi135 – 1384
Helixi141 – 1444
Beta strandi150 – 1523
Helixi161 – 1633
Helixi169 – 1713
Beta strandi173 – 1753
Turni176 – 1783
Beta strandi179 – 1813
Helixi184 – 1874
Beta strandi193 – 1953

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WGCX-ray2.20A/B29-198[»]
9WGAX-ray1.80A/B29-198[»]
ProteinModelPortaliP02876.
SMRiP02876. Positions 28-198.

Miscellaneous databases

EvolutionaryTraceiP02876.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 6942Chitin-binding type-1 1
Add
BLAST
Domaini70 – 11243Chitin-binding type-1 2
Add
BLAST
Domaini113 – 15543Chitin-binding type-1 3
Add
BLAST
Domaini156 – 19843Chitin-binding type-1 4
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni37 – 393Substrate binding By similarity
Regioni89 – 10012Substrate binding By similarity
Add
BLAST
Regioni141 – 1422Substrate binding

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di3.30.60.10. 4 hits.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 4 hits.
[Graphical view]
PRINTSiPR00451. CHITINBINDNG.
ProDomiPD000609. Chitin_bd_1. 3 hits.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 4 hits.
[Graphical view]
SUPFAMiSSF57016. SSF57016. 4 hits.
PROSITEiPS00026. CHIT_BIND_I_1. 4 hits.
PS50941. CHIT_BIND_I_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02876-1 [UniParc]FASTAAdd to Basket

« Hide

MRKMMSTMAL TLGAAVFLAF AAATAQAQRC GEQGSNMECP NNLCCSQYGY    50
CGMGGDYCGK GCQNGACWTS KRCGSQAGGA TCPNNHCCSQ YGHCGFGAEY 100
CGAGCQGGPC RADIKCGSQS GGKLCPNNLC CSQWGFCGLG SEFCGGGCQS 150
GACSTDKPCG KDAGGRVCTN NYCCSKWGSC GIGPGYCGAG CQSGGCDAVF 200
AGAITANSTL LAE 213
Length:213
Mass (Da):21,356
Last modified:November 1, 1990 - v3
Checksum:iF656A5C9277148AF
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti64 – 641N → D AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M25537 mRNA. Translation: AAA34258.1.
PIRiS09624. AEWT2.
UniGeneiTa.147.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M25537 mRNA. Translation: AAA34258.1 .
PIRi S09624. AEWT2.
UniGenei Ta.147.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2WGC X-ray 2.20 A/B 29-198 [» ]
9WGA X-ray 1.80 A/B 29-198 [» ]
ProteinModelPortali P02876.
SMRi P02876. Positions 28-198.
ModBasei Search...

Protein family/group databases

Allergomei 650. Tri a 18.
CAZyi CBM18. Carbohydrate-Binding Module Family 18.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei P02876.

Miscellaneous databases

EvolutionaryTracei P02876.
PROi P02876.

Family and domain databases

Gene3Di 3.30.60.10. 4 hits.
InterProi IPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
[Graphical view ]
Pfami PF00187. Chitin_bind_1. 4 hits.
[Graphical view ]
PRINTSi PR00451. CHITINBINDNG.
ProDomi PD000609. Chitin_bd_1. 3 hits.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00270. ChtBD1. 4 hits.
[Graphical view ]
SUPFAMi SSF57016. SSF57016. 4 hits.
PROSITEi PS00026. CHIT_BIND_I_1. 4 hits.
PS50941. CHIT_BIND_I_2. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequences of cDNA clones encoding wheat germ agglutinin isolectins A and D."
    Smith J.J., Raikhel N.V.
    Plant Mol. Biol. 13:601-603(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Primary structure of wheat germ agglutinin isolectin 2. Peptide order deduced from X-ray structure."
    Wright C.S., Gavilanes F., Peterson D.L.
    Biochemistry 23:280-287(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-198.
    Tissue: Germ.
  3. "Sequence variability in three wheat germ agglutinin isolectins: products of multiple genes in polyploid wheat."
    Wright C.S., Raikhel N.V.
    J. Mol. Evol. 28:327-336(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 68; 136; 161 AND 177.
  4. "The crystal structure of wheat germ agglutinin at 2.2-A resolution."
    Wright C.S.
    J. Mol. Biol. 111:439-457(1977) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
  5. "Refinement of the crystal structure of wheat germ agglutinin isolectin 2 at 1.8-A resolution."
    Wright C.S.
    J. Mol. Biol. 194:501-529(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
  6. Erratum
    Wright C.S.
    J. Mol. Biol. 199:239-239(1988)

Entry informationi

Entry nameiAGI2_WHEAT
AccessioniPrimary (citable) accession number: P02876
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1990
Last modified: April 16, 2014
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The 4 sites proposed for binding to carbohydrates (N-acetyl-D-glucosamine) of receptor molecules are on the surface of the agglutinin molecule.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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