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Protein

Agglutinin isolectin 2

Gene
N/A
Organism
Triticum aestivum (Wheat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin.

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

Chitin-binding, Lectin

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Agglutinin isolectin 2
Alternative name(s):
Isolectin D
WGA2
OrganismiTriticum aestivum (Wheat)
Taxonomic identifieri4565 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeTriticinaeTriticum
Proteomesi
  • UP000019116 Componenti: Unassembled WGS sequence

Pathology & Biotechi

Protein family/group databases

Allergomei650. Tri a 18.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 271 PublicationAdd BLAST27
ChainiPRO_000000525728 – 198Agglutinin isolectin 2Add BLAST171
PropeptideiPRO_0000005258199 – 213Add BLAST15

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei28Pyrrolidone carboxylic acid1 Publication1
Disulfide bondi30 ↔ 45PROSITE-ProRule annotation1 Publication
Disulfide bondi39 ↔ 51PROSITE-ProRule annotation1 Publication
Disulfide bondi44 ↔ 58PROSITE-ProRule annotation1 Publication
Disulfide bondi62 ↔ 67PROSITE-ProRule annotation1 Publication
Disulfide bondi73 ↔ 88PROSITE-ProRule annotation1 Publication
Disulfide bondi82 ↔ 94PROSITE-ProRule annotation1 Publication
Disulfide bondi87 ↔ 101PROSITE-ProRule annotation1 Publication
Disulfide bondi105 ↔ 110PROSITE-ProRule annotation1 Publication
Disulfide bondi116 ↔ 131PROSITE-ProRule annotation1 Publication
Disulfide bondi125 ↔ 137PROSITE-ProRule annotation1 Publication
Disulfide bondi130 ↔ 144PROSITE-ProRule annotation1 Publication
Disulfide bondi148 ↔ 153PROSITE-ProRule annotation1 Publication
Disulfide bondi159 ↔ 174PROSITE-ProRule annotation1 Publication
Disulfide bondi168 ↔ 180PROSITE-ProRule annotation1 Publication
Disulfide bondi173 ↔ 187PROSITE-ProRule annotation1 Publication
Disulfide bondi191 ↔ 196PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

Homodimer, u-shaped.

Structurei

Secondary structure

1213
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi31 – 34Combined sources4
Helixi40 – 42Combined sources3
Helixi55 – 58Combined sources4
Beta strandi64 – 66Combined sources3
Helixi74 – 77Combined sources4
Helixi83 – 85Combined sources3
Beta strandi92 – 95Combined sources4
Helixi98 – 101Combined sources4
Beta strandi107 – 109Combined sources3
Helixi118 – 120Combined sources3
Helixi126 – 128Combined sources3
Beta strandi135 – 138Combined sources4
Helixi141 – 144Combined sources4
Beta strandi150 – 152Combined sources3
Helixi161 – 163Combined sources3
Helixi169 – 171Combined sources3
Beta strandi173 – 175Combined sources3
Turni176 – 178Combined sources3
Beta strandi179 – 181Combined sources3
Helixi184 – 187Combined sources4
Beta strandi193 – 195Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WGCX-ray2.20A/B29-198[»]
9WGAX-ray1.80A/B29-198[»]
ProteinModelPortaliP02876.
SMRiP02876.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02876.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 69Chitin-binding type-1 1PROSITE-ProRule annotationAdd BLAST42
Domaini70 – 112Chitin-binding type-1 2PROSITE-ProRule annotationAdd BLAST43
Domaini113 – 155Chitin-binding type-1 3PROSITE-ProRule annotationAdd BLAST43
Domaini156 – 198Chitin-binding type-1 4PROSITE-ProRule annotationAdd BLAST43

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni37 – 39Substrate bindingBy similarity3
Regioni89 – 100Substrate bindingBy similarityAdd BLAST12
Regioni141 – 142Substrate binding2

Sequence similaritiesi

Contains 4 chitin-binding type-1 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di3.30.60.10. 4 hits.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 4 hits.
[Graphical view]
PRINTSiPR00451. CHITINBINDNG.
ProDomiPD000609. Chitin_bd_1. 3 hits.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 4 hits.
[Graphical view]
SUPFAMiSSF57016. SSF57016. 4 hits.
PROSITEiPS00026. CHIT_BIND_I_1. 4 hits.
PS50941. CHIT_BIND_I_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02876-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKMMSTMAL TLGAAVFLAF AAATAQAQRC GEQGSNMECP NNLCCSQYGY
60 70 80 90 100
CGMGGDYCGK GCQNGACWTS KRCGSQAGGA TCPNNHCCSQ YGHCGFGAEY
110 120 130 140 150
CGAGCQGGPC RADIKCGSQS GGKLCPNNLC CSQWGFCGLG SEFCGGGCQS
160 170 180 190 200
GACSTDKPCG KDAGGRVCTN NYCCSKWGSC GIGPGYCGAG CQSGGCDAVF
210
AGAITANSTL LAE
Length:213
Mass (Da):21,356
Last modified:November 1, 1990 - v3
Checksum:iF656A5C9277148AF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti64N → D AA sequence (PubMed:6546522).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25537 mRNA. Translation: AAA34258.1.
PIRiS09624. AEWT2.
UniGeneiTa.147.

Genome annotation databases

EnsemblPlantsiTRIAE_CS42_1DL_TGACv1_063532_AA0228320.1; TRIAE_CS42_1DL_TGACv1_063532_AA0228320.1; TRIAE_CS42_1DL_TGACv1_063532_AA0228320.
GrameneiTRIAE_CS42_1DL_TGACv1_063532_AA0228320.1; TRIAE_CS42_1DL_TGACv1_063532_AA0228320.1; TRIAE_CS42_1DL_TGACv1_063532_AA0228320.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25537 mRNA. Translation: AAA34258.1.
PIRiS09624. AEWT2.
UniGeneiTa.147.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WGCX-ray2.20A/B29-198[»]
9WGAX-ray1.80A/B29-198[»]
ProteinModelPortaliP02876.
SMRiP02876.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei650. Tri a 18.
CAZyiCBM18. Carbohydrate-Binding Module Family 18.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiTRIAE_CS42_1DL_TGACv1_063532_AA0228320.1; TRIAE_CS42_1DL_TGACv1_063532_AA0228320.1; TRIAE_CS42_1DL_TGACv1_063532_AA0228320.
GrameneiTRIAE_CS42_1DL_TGACv1_063532_AA0228320.1; TRIAE_CS42_1DL_TGACv1_063532_AA0228320.1; TRIAE_CS42_1DL_TGACv1_063532_AA0228320.

Miscellaneous databases

EvolutionaryTraceiP02876.
PROiP02876.

Family and domain databases

Gene3Di3.30.60.10. 4 hits.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 4 hits.
[Graphical view]
PRINTSiPR00451. CHITINBINDNG.
ProDomiPD000609. Chitin_bd_1. 3 hits.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 4 hits.
[Graphical view]
SUPFAMiSSF57016. SSF57016. 4 hits.
PROSITEiPS00026. CHIT_BIND_I_1. 4 hits.
PS50941. CHIT_BIND_I_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGI2_WHEAT
AccessioniPrimary (citable) accession number: P02876
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1990
Last modified: November 30, 2016
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The 4 sites proposed for binding to carbohydrates (N-acetyl-D-glucosamine) of receptor molecules are on the surface of the agglutinin molecule.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.