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Protein

Alpha-amylase inhibitor 1

Gene

LLP

Organism
Phaseolus vulgaris (Kidney bean) (French bean)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Lectin and alpha-amylase inhibitor. Acts as a defensive protein against insects.1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Alpha-amylase inhibitor

Keywords - Ligandi

Lectin

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase inhibitor 1
Short name:
Alpha-AI-1
Short name:
Alpha-AI1
Alternative name(s):
Lectin
Cleaved into the following 2 chains:
Gene namesi
Name:LLP
Synonyms:Alpha-AI1
OrganismiPhaseolus vulgaris (Kidney bean) (French bean)
Taxonomic identifieri3885 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaePhaseolus

Pathology & Biotechi

Biotechnological usei

Sold as a diet aid under the name of 'Phaseolamin' or 'Phase 2'.

Protein family/group databases

Allergomei2946. Pha v aAI.
8208. Pha v aAI.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 233 PublicationsAdd BLAST23
ChainiPRO_000001762724 – 100Alpha-amylase inhibitor 1 chain 1Add BLAST77
ChainiPRO_0000017628101 – 239Alpha-amylase inhibitor 1 chain 2Add BLAST139
PropeptideiPRO_0000017629240 – 2467

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi35N-linked (GlcNAc...)1 Publication1
Glycosylationi88N-linked (GlcNAc...)1 Publication1
Glycosylationi163N-linked (GlcNAc...)1 Publication1

Post-translational modificationi

Proteolytic processing yields active form.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP02873.

PTM databases

UniCarbKBiP02873.

Interactioni

Subunit structurei

Heterodimer of chain 1 and chain 2.1 Publication

Protein-protein interaction databases

MINTiMINT-88091.

Structurei

Secondary structure

1246
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi28 – 33Combined sources6
Helixi36 – 38Combined sources3
Beta strandi39 – 47Combined sources9
Beta strandi53 – 58Combined sources6
Beta strandi60 – 70Combined sources11
Beta strandi72 – 75Combined sources4
Turni77 – 79Combined sources3
Beta strandi84 – 94Combined sources11
Beta strandi103 – 111Combined sources9
Beta strandi121 – 126Combined sources6
Turni127 – 130Combined sources4
Beta strandi131 – 136Combined sources6
Beta strandi139 – 145Combined sources7
Helixi148 – 150Combined sources3
Turni151 – 153Combined sources3
Beta strandi156 – 163Combined sources8
Turni164 – 167Combined sources4
Beta strandi168 – 174Combined sources7
Turni176 – 178Combined sources3
Beta strandi181 – 187Combined sources7
Helixi193 – 195Combined sources3
Beta strandi196 – 206Combined sources11
Helixi209 – 211Combined sources3
Beta strandi215 – 226Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DHKX-ray1.85B24-246[»]
1VIWX-ray3.00B24-228[»]
ProteinModelPortaliP02873.
SMRiP02873.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02873.

Family & Domainsi

Sequence similaritiesi

Belongs to the leguminous lectin family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR016363. L-lectin.
IPR000985. Lectin_LegA_CS.
IPR019825. Lectin_legB_Mn/Ca_BS.
IPR001220. Legume_lectin_dom.
[Graphical view]
PfamiPF00139. Lectin_legB. 1 hit.
[Graphical view]
PIRSFiPIRSF002690. L-type_lectin_plant. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS00308. LECTIN_LEGUME_ALPHA. 1 hit.
PS00307. LECTIN_LEGUME_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02873-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIMASSKLLS LALFLALLSH ANSATETSFI IDAFNKTNLI LQGDATVSSN
60 70 80 90 100
GNLQLSYNSY DSMSRAFYSA PIQIRDSTTG NVASFDTNFT MNIRTHRQAN
110 120 130 140 150
SAVGLDFVLV PVQPESKGDT VTVEFDTFLS RISIDVNNND IKSVPWDVHD
160 170 180 190 200
YDGQNAEVRI TYNSSTKVFS VSLSNPSTGK SNNVSTTVEL EKEVYDWVSV
210 220 230 240
GFSATSGAYQ WSYETHDVLS WSFSSKFINL KDQKSERSNI VLNKIL
Length:246
Mass (Da):27,207
Last modified:July 21, 1986 - v1
Checksum:iEA56F15EF1F78788
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30I → N AA sequence (PubMed:7763497).Curated1
Sequence conflicti73Q → R in CAD28835 (Ref. 3) Curated1
Sequence conflicti142K → E in CAD28835 (Ref. 3) Curated1
Sequence conflicti196D → N (PubMed:6897567).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01261 Genomic DNA. Translation: AAA33769.1.
AJ439614 Genomic DNA. Translation: CAD28835.1.
PIRiA03365. LNFB.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01261 Genomic DNA. Translation: AAA33769.1.
AJ439614 Genomic DNA. Translation: CAD28835.1.
PIRiA03365. LNFB.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DHKX-ray1.85B24-246[»]
1VIWX-ray3.00B24-228[»]
ProteinModelPortaliP02873.
SMRiP02873.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-88091.

Protein family/group databases

Allergomei2946. Pha v aAI.
8208. Pha v aAI.0101.

PTM databases

UniCarbKBiP02873.

Proteomic databases

PRIDEiP02873.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP02873.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR016363. L-lectin.
IPR000985. Lectin_LegA_CS.
IPR019825. Lectin_legB_Mn/Ca_BS.
IPR001220. Legume_lectin_dom.
[Graphical view]
PfamiPF00139. Lectin_legB. 1 hit.
[Graphical view]
PIRSFiPIRSF002690. L-type_lectin_plant. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS00308. LECTIN_LEGUME_ALPHA. 1 hit.
PS00307. LECTIN_LEGUME_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEA1_PHAVU
AccessioniPrimary (citable) accession number: P02873
Secondary accession number(s): Q8RVY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.