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P02872

- LECG_ARAHY

UniProt

P02872 - LECG_ARAHY

Protein

Galactose-binding lectin

Gene
N/A
Organism
Arachis hypogaea (Peanut)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 3 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    D-galactose specific lectin.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi144 – 1441Manganese
    Metal bindingi146 – 1461Calcium
    Metal bindingi146 – 1461Manganese
    Metal bindingi148 – 1481Calcium; via carbonyl oxygen
    Metal bindingi150 – 1501Calcium
    Metal bindingi155 – 1551Calcium
    Metal bindingi155 – 1551Manganese
    Metal bindingi160 – 1601Manganese

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Ligandi

    Calcium, Lectin, Manganese, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Galactose-binding lectin
    Alternative name(s):
    Agglutinin
    PNA
    OrganismiArachis hypogaea (Peanut)
    Taxonomic identifieri3818 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeDalbergieaeArachis

    Pathology & Biotechi

    Protein family/group databases

    Allergomei1050. Ara h Agglutinin.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 23231 PublicationAdd
    BLAST
    Chaini24 – 273250Galactose-binding lectinPRO_0000017583Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.

    Structurei

    Secondary structure

    1
    273
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi25 – 339
    Beta strandi41 – 466
    Beta strandi51 – 533
    Beta strandi60 – 645
    Beta strandi66 – 738
    Turni80 – 823
    Beta strandi87 – 9711
    Beta strandi100 – 1034
    Beta strandi107 – 1148
    Helixi126 – 1283
    Turni129 – 1313
    Beta strandi136 – 14611
    Helixi151 – 1533
    Beta strandi160 – 16910
    Beta strandi171 – 1755
    Beta strandi183 – 1919
    Turni192 – 1954
    Beta strandi196 – 2027
    Beta strandi204 – 2063
    Beta strandi208 – 2147
    Helixi217 – 2204
    Beta strandi223 – 23210
    Beta strandi235 – 2373
    Beta strandi240 – 25213

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BZWX-ray2.70A/B/C/D24-255[»]
    1CIWX-ray2.70A/B/C/D24-259[»]
    1CQ9X-ray3.50A/B/C/D24-259[»]
    1CR7X-ray2.60A/B/C/D/E/F/G/H24-259[»]
    1QF3X-ray2.80A/B/C/D24-259[»]
    1RIRX-ray2.90A/B/C/D24-259[»]
    1RITX-ray2.85A/B/C/D24-259[»]
    1V6IX-ray2.15A/B/C/D24-255[»]
    1V6JX-ray2.90A/B/C/D24-255[»]
    1V6KX-ray2.40A/B/C/D24-255[»]
    1V6LX-ray2.50A/B/C/D24-255[»]
    1V6MX-ray2.70A/B/C/D/E/F/G/H24-255[»]
    1V6NX-ray3.50A/B/C/D/E/F/G/H24-255[»]
    1V6OX-ray3.00A/B/C/D/E/F/G/H24-255[»]
    2DH1X-ray7.65A/B/C/D24-259[»]
    2DV9X-ray2.48A/B/C/D24-259[»]
    2DVAX-ray2.20A/B/C/D24-259[»]
    2DVBX-ray2.25A/B/C/D24-259[»]
    2DVDX-ray2.25A/B/C/D24-259[»]
    2DVFX-ray2.74A/B/C/D24-259[»]
    2DVGX-ray2.78A/B/C/D24-259[»]
    2PELX-ray2.25A/B/C/D24-259[»]
    2TEPX-ray2.50A/B/C/D24-259[»]
    ProteinModelPortaliP02872.
    SMRiP02872. Positions 24-255.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP02872.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the leguminous lectin family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR016363. Lectin.
    IPR000985. Lectin_LegA_CS.
    IPR019825. Lectin_legB_Mn/Ca_BS.
    IPR001220. Legume_lectin_dom.
    [Graphical view]
    PfamiPF00139. Lectin_legB. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002690. L-type_lectin_plant. 1 hit.
    SUPFAMiSSF49899. SSF49899. 1 hit.
    PROSITEiPS00308. LECTIN_LEGUME_ALPHA. 1 hit.
    PS00307. LECTIN_LEGUME_BETA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P02872-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKPFCVFLTF FLLLAASSKK VDSAETVSFN FNSFSEGNPA INFQGDVTVL    50
    SNGNIQLTNL NKVNSVGRVL YAMPVRIWSS ATGNVASFLT SFSFEMKDIK 100
    DYDPADGIIF FIAPEDTQIP AGSIGGGTLG VSDTKGAGHF VGVEFDTYSN 150
    SEYNDPPTDH VGIDVNSVDS VKTVPWNSVS GAVVKVTVIY DSSTKTLSVA 200
    VTNDNGDITT IAQVVDLKAK LPERVKFGFS ASGSLGGRQI HLIRSWSFTS 250
    TLITTTRRSI DNNEKKIMNM ASA 273
    Length:273
    Mass (Da):29,325
    Last modified:October 1, 1996 - v3
    Checksum:i05A0B1A8FAC7B159
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti115 – 1151E → V in minor form.
    Natural varianti172 – 1721K → A in minor form.
    Natural varianti185 – 1851K → I in minor form.
    Natural varianti235 – 2362LG → RA in minor form.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S42352 mRNA. Translation: AAB22817.1.
    PIRiA03364. LNNPG.
    S24044.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S42352 mRNA. Translation: AAB22817.1 .
    PIRi A03364. LNNPG.
    S24044.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BZW X-ray 2.70 A/B/C/D 24-255 [» ]
    1CIW X-ray 2.70 A/B/C/D 24-259 [» ]
    1CQ9 X-ray 3.50 A/B/C/D 24-259 [» ]
    1CR7 X-ray 2.60 A/B/C/D/E/F/G/H 24-259 [» ]
    1QF3 X-ray 2.80 A/B/C/D 24-259 [» ]
    1RIR X-ray 2.90 A/B/C/D 24-259 [» ]
    1RIT X-ray 2.85 A/B/C/D 24-259 [» ]
    1V6I X-ray 2.15 A/B/C/D 24-255 [» ]
    1V6J X-ray 2.90 A/B/C/D 24-255 [» ]
    1V6K X-ray 2.40 A/B/C/D 24-255 [» ]
    1V6L X-ray 2.50 A/B/C/D 24-255 [» ]
    1V6M X-ray 2.70 A/B/C/D/E/F/G/H 24-255 [» ]
    1V6N X-ray 3.50 A/B/C/D/E/F/G/H 24-255 [» ]
    1V6O X-ray 3.00 A/B/C/D/E/F/G/H 24-255 [» ]
    2DH1 X-ray 7.65 A/B/C/D 24-259 [» ]
    2DV9 X-ray 2.48 A/B/C/D 24-259 [» ]
    2DVA X-ray 2.20 A/B/C/D 24-259 [» ]
    2DVB X-ray 2.25 A/B/C/D 24-259 [» ]
    2DVD X-ray 2.25 A/B/C/D 24-259 [» ]
    2DVF X-ray 2.74 A/B/C/D 24-259 [» ]
    2DVG X-ray 2.78 A/B/C/D 24-259 [» ]
    2PEL X-ray 2.25 A/B/C/D 24-259 [» ]
    2TEP X-ray 2.50 A/B/C/D 24-259 [» ]
    ProteinModelPortali P02872.
    SMRi P02872. Positions 24-255.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 1050. Ara h Agglutinin.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P02872.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR016363. Lectin.
    IPR000985. Lectin_LegA_CS.
    IPR019825. Lectin_legB_Mn/Ca_BS.
    IPR001220. Legume_lectin_dom.
    [Graphical view ]
    Pfami PF00139. Lectin_legB. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002690. L-type_lectin_plant. 1 hit.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    PROSITEi PS00308. LECTIN_LEGUME_ALPHA. 1 hit.
    PS00307. LECTIN_LEGUME_BETA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequence analysis and expression in Escherichia coli of the cDNA encoding a precursor of peanut agglutinin."
      Rodriguez-Arango E., Arango R., Adar R., Galili G., Sharon N.
      FEBS Lett. 307:185-189(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Seed.
    2. Cited for: PROTEIN SEQUENCE OF 24-273.
      Tissue: Seed.
    3. "Sequence studies of peanut agglutinin."
      Lauwereys M., Foriers A., Sharon N., Strosberg A.D.
      FEBS Lett. 181:241-244(1985)
      Cited for: PRELIMINARY PARTIAL PROTEIN SEQUENCE.
      Strain: cv. Shulamit.
    4. "Conformation, protein-carbohydrate interactions and a novel subunit association in the refined structure of peanut lectin-lactose complex."
      Banerjee R., Das K., Ravishankar R., Suguna K., Surolia A., Vijayan M.
      J. Mol. Biol. 259:281-296(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS).
    5. "Structures of the complexes of peanut lectin with methyl-beta-galactose and N-acetyllactosamine and a comparative study of carbohydrate binding in Gal/GalNAc-specific legume lectins."
      Ravishankar R., Suguna K., Surolia A., Vijayan M.
      Acta Crystallogr. D 55:1375-1382(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).

    Entry informationi

    Entry nameiLECG_ARAHY
    AccessioniPrimary (citable) accession number: P02872
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 102 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Binds one manganese (or another transition metal) ion and one calcium ion. The metal ions are essential for the saccharide-binding and cell-agglutinating activities.

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3