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Protein

Lectin

Gene

LECA

Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

D-mannose specific lectin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi149ManganeseBy similarity1
Metal bindingi151CalciumBy similarity1
Metal bindingi151ManganeseBy similarity1
Metal bindingi153Calcium; via carbonyl oxygenBy similarity1
Metal bindingi155CalciumBy similarity1
Metal bindingi159CalciumBy similarity1
Metal bindingi159ManganeseBy similarity1
Metal bindingi166ManganeseBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

Calcium, Lectin, Manganese, Mannose-binding, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lectin
Cleaved into the following 2 chains:
Gene namesi
Name:LECA
Synonyms:PSL1
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Pathology & Biotechi

Protein family/group databases

Allergomei8818. Pis s Agglutinin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Add BLAST30
ChainiPRO_000001762331 – 217Lectin beta chainAdd BLAST187
ChainiPRO_0000017624218 – 275Lectin alpha chainAdd BLAST58

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi217N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP02867.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.

Protein-protein interaction databases

MINTiMINT-1508409.

Structurei

Secondary structure

1275
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi32 – 40Combined sources9
Beta strandi48 – 52Combined sources5
Beta strandi55 – 57Combined sources3
Beta strandi58 – 64Combined sources7
Beta strandi71 – 78Combined sources8
Turni85 – 87Combined sources3
Beta strandi92 – 102Combined sources11
Beta strandi104 – 108Combined sources5
Beta strandi112 – 119Combined sources8
Helixi128 – 130Combined sources3
Turni131 – 133Combined sources3
Helixi141 – 143Combined sources3
Beta strandi146 – 151Combined sources6
Turni156 – 158Combined sources3
Beta strandi166 – 175Combined sources10
Beta strandi177 – 181Combined sources5
Beta strandi190 – 197Combined sources8
Turni198 – 201Combined sources4
Beta strandi202 – 209Combined sources8
Beta strandi219 – 226Combined sources8
Helixi229 – 232Combined sources4
Beta strandi235 – 244Combined sources10
Beta strandi246 – 248Combined sources3
Beta strandi251 – 263Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BQPX-ray2.10A/C31-211[»]
B/D218-264[»]
1HKDX-ray2.09A/C31-211[»]
B/D218-269[»]
1OFSX-ray1.80A/C31-217[»]
B/D218-265[»]
1RINX-ray2.60A/C31-210[»]
B/D218-266[»]
2BQPX-ray1.90A/B31-264[»]
2LTNX-ray1.70A/C31-211[»]
B/D218-269[»]
ProteinModelPortaliP02867.
SMRiP02867.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02867.

Family & Domainsi

Sequence similaritiesi

Belongs to the leguminous lectin family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR016363. L-lectin.
IPR000985. Lectin_LegA_CS.
IPR019825. Lectin_legB_Mn/Ca_BS.
IPR001220. Legume_lectin_dom.
[Graphical view]
PfamiPF00139. Lectin_legB. 1 hit.
[Graphical view]
PIRSFiPIRSF002690. L-type_lectin_plant. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS00308. LECTIN_LEGUME_ALPHA. 1 hit.
PS00307. LECTIN_LEGUME_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02867-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLQTQMIS FYAIFLSILL TTILFFKVNS TETTSFLITK FSPDQQNLIF
60 70 80 90 100
QGDGYTTKEK LTLTKAVKNT VGRALYSSPI HIWDRETGNV ANFVTSFTFV
110 120 130 140 150
INAPNSYNVA DGFTFFIAPV DTKPQTGGGY LGVFNSAEYD KTTQTVAVEF
160 170 180 190 200
DTFYNAAWDP SNRDRHIGID VNSIKSVNTK SWKLQNGEEA NVVIAFNAAT
210 220 230 240 250
NVLTVSLTYP NSLEEENVTS YTLSDVVSLK DVVPEWVRIG FSATTGAEYA
260 270
AHEVLSWSFH SELSGTSSSK QAADA
Length:275
Mass (Da):30,270
Last modified:July 21, 1986 - v1
Checksum:iFD7BE8E00A811222
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti269Missing (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00440 Genomic DNA. Translation: CAA68497.1.
M18160 mRNA. Translation: AAA33676.1.
J01254 mRNA. No translation available.
X66368 Genomic DNA. Translation: CAA47011.1.
PIRiA26844. LNPM.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00440 Genomic DNA. Translation: CAA68497.1.
M18160 mRNA. Translation: AAA33676.1.
J01254 mRNA. No translation available.
X66368 Genomic DNA. Translation: CAA47011.1.
PIRiA26844. LNPM.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BQPX-ray2.10A/C31-211[»]
B/D218-264[»]
1HKDX-ray2.09A/C31-211[»]
B/D218-269[»]
1OFSX-ray1.80A/C31-217[»]
B/D218-265[»]
1RINX-ray2.60A/C31-210[»]
B/D218-266[»]
2BQPX-ray1.90A/B31-264[»]
2LTNX-ray1.70A/C31-211[»]
B/D218-269[»]
ProteinModelPortaliP02867.
SMRiP02867.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1508409.

Protein family/group databases

Allergomei8818. Pis s Agglutinin.

Proteomic databases

PRIDEiP02867.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP02867.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR016363. L-lectin.
IPR000985. Lectin_LegA_CS.
IPR019825. Lectin_legB_Mn/Ca_BS.
IPR001220. Legume_lectin_dom.
[Graphical view]
PfamiPF00139. Lectin_legB. 1 hit.
[Graphical view]
PIRSFiPIRSF002690. L-type_lectin_plant. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS00308. LECTIN_LEGUME_ALPHA. 1 hit.
PS00307. LECTIN_LEGUME_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEC_PEA
AccessioniPrimary (citable) accession number: P02867
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds one manganese (or another transition metal) ion and one calcium ion. The metal ions are essential for the saccharide-binding and cell-agglutinating activities.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.