P02863 (GDA0_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Alpha/beta-gliadin Alternative name(s): Prolamin
Organism	Triticum aestivum (Wheat)
Taxonomic identifier	4565 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Gliadin is the major seed storage protein in wheat.
Post-translational modification	Substrate of transglutaminase By similarity.
Allergenic properties	Causes an allergic reaction in human. Is the cause of the celiac disease, also known as celiac sprue or gluten-sensitive enteropathy By similarity.
Miscellaneous	The alpha/beta-gliadins can be divided into 5 homology classes. Sequence divergence between the classes is due to single base substitutions and to duplications or deletions within or near direct repeats. There are more than a 100 copies of the gene for alpha/beta-gliadin per haploid genome.
Sequence similarities	Belongs to the gliadin/glutenin family.

Ontologies

Keywords
Disease	Allergen
Domain	Repeat Signal
Molecular function	Seed storage protein Storage protein
Gene Ontology (GO)
Molecular_function	nutrient reservoir activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20
	Chain	21 – 286	266	Alpha/beta-gliadin		PRO_0000032267
Experimental info
	Sequence conflict	37	1	Q → L Ref.1
	Sequence conflict	93	1	P → Q in CAA26384. Ref.2
	Sequence conflict	193 – 194	2	HN → LK in CAA25593. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P02863 [UniParc]. Last modified July 21, 1986. Version 2. Checksum: E5EBD3BBE292E0C6	FASTA	286	32,963
	10 20 30 40 50 60 MKTFLILVLL AIVATTATTA VRFPVPQLQP QNPSQQQPQE QVPLVQQQQF LGQQQPFPPQ 70 80 90 100 110 120 QPYPQPQPFP SQLPYLQLQP FPQPQLPYSQ PQPFRPQQPY PQPQPQYSQP QQPISQQQQQ 130 140 150 160 170 180 QQQQQQQQQQ QQQILQQILQ QQLIPCMDVV LQQHNIAHGR SQVLQQSTYQ LLQELCCQHL 190 200 210 220 230 240 WQIPEQSQCQ AIHNVVHAII LHQQQKQQQQ PSSQVSFQQP LQQYPLGQGS FRPSQQNPQA 250 260 270 280 QGSVQPQQLP QFEEIRNLAL QTLPAMCNVY IPPYCTIAPF GIFGTN

References

[1]	"Developmentally regulated plant genes: the nucleotide sequence of a wheat gliadin genomic clone." Rafalski J.A., Scheets K., Metzler M., Peterson D.M., Hedgcoth C., Soll D.G. EMBO J. 3:1409-1415(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Newton.
[2]	"Conservation and variability of wheat alpha/beta-gliadin genes." Sumner-Smith M., Rafalski J.A., Sugiyama T., Stoll M., Soell D. Nucleic Acids Res. 13:3905-3916(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Nucleic acid sequence and chromosome assignment of a wheat storage protein gene." Anderson O.D., Litts J.C., Gautier M.-F., Greene F.C. Nucleic Acids Res. 12:8129-8144(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	K03076 mRNA. Translation: AAA34280.1. X02539 Genomic DNA. Translation: CAA26384.1. X01130 Genomic DNA. Translation: CAA25593.1.
PIR	EEWTA. A03354. S07923.
UniGene	Ta.69925.
3D structure databases
ProteinModelPortal	P02863.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P02863.
Family and domain databases
Gene3D	1.10.120.10. 2 hits.
InterPro	IPR016140. Bifunc_inhib/LTP/seed_store. IPR001954. Glia_glutenin. IPR001376. Gliadin. IPR013771. Trypsin/amylase_inhib. [Graphical view]
Pfam	PF00234. Tryp_alpha_amyl. 1 hit. [Graphical view]
PRINTS	PR00208. GLIADGLUTEN. PR00209. GLIADIN.
SMART	SM00499. AAI. 1 hit. [Graphical view]
SUPFAM	SSF47699. Bifunc_inhib/LTP/seed_store. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GDA0_WHEAT

Accession

Primary (citable) accession number: P02863

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program