P02858 (GLYG4_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycinin G4

Cleaved into the following 3 chains:

1. Glycinin A5 subunit
2. Glycinin A4 subunit
3. Glycinin B3 subunit

Gene names

Name:

GY4

Organism

Glycine max (Soybean) (Glycine hispida)

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Glycinin is the major seed storage protein of soybean.
Subunit structure	Hexamer; each subunit is composed of an acidic and a basic chain derived from a single precursor and linked by a disulfide bond. Ref.2
Sequence similarities	Belongs to the 11S seed storage protein (globulins) family.

Ontologies

Keywords
Domain	Signal
Molecular function	Seed storage protein Storage protein
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	nutrient reservoir activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23
	Chain	24 – 120	97	Glycinin A5 subunit		PRO_0000032020
	Chain	121 – 377	257	Glycinin A4 subunit		PRO_0000032021
	Chain	378 – 562	185	Glycinin B3 subunit		PRO_0000032022
Amino acid modifications
	Disulfide bond	32 ↔ 65		By similarity
	Disulfide bond	108 ↔ 384		Interchain (between A5 and B3 chains) By similarity
Experimental info
	Sequence conflict	29	1	L → F AA sequence Ref.2
	Sequence conflict	82	1	S → L AA sequence Ref.2
	Sequence conflict	86	1	S → L AA sequence Ref.2
	Sequence conflict	94	1	I → V AA sequence Ref.2
	Sequence conflict	101 – 103	3	LGV → IGM AA sequence Ref.2
	Sequence conflict	105	1	I → F AA sequence Ref.2
	Sequence conflict	117	1	E → Q AA sequence Ref.2
	Sequence conflict	253	1	E → Q in CAB57802. Ref.3
	Sequence conflict	332 – 335	4	NKTG → EQDQD in CAB57802. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P02858 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: F5A06B8856B9BBD6	FASTA	562	63,587
	10 20 30 40 50 60 MGKPFTLSLS SLCLLLLSSA CFAISSSKLN ECQLNNLNAL EPDHRVESEG GLIQTWNSQH 70 80 90 100 110 120 PELKCAGVTV SKLTLNRNGL HSPSYSPYPR MIIIAQGKGA LGVAIPGCPE TFEEPQEQSN 130 140 150 160 170 180 RRGSRSQKQQ LQDSHQKIRH FNEGDVLVIP PSVPYWTYNT GDEPVVAISL LDTSNFNNQL 190 200 210 220 230 240 DQTPRVFYLA GNPDIEYPET MQQQQQQKSH GGRKQGQHQQ EEEEEGGSVL SGFSKHFLAQ 250 260 270 280 290 300 SFNTNEDIAE KLESPDDERK QIVTVEGGLS VISPKWQEQQ DEDEDEDEDD EDEQIPSHPP 310 320 330 340 350 360 RRPSHGKREQ DEDEDEDEDK PRPSRPSQGK RNKTGQDEDE DEDEDQPRKS REWRSKKTQP 370 380 390 400 410 420 RRPRQEEPRE RGCETRNGVE ENICTLKLHE NIARPSRADF YNPKAGRIST LNSLTLPALR 430 440 450 460 470 480 QFQLSAQYVV LYKNGIYSPH WNLNANSVIY VTRGQGKVRV VNCQGNAVFD GELRRGQLLV 490 500 510 520 530 540 VPQNFVVAEQ AGEQGFEYIV FKTHHNAVTS YLKDVFRAIP SEVLAHSYNL RQSQVSELKY 550 560 EGNWGPLVNP ESQQGSPRVK VA

References

[1]	"Glycinin A5A4B3 mRNA: cDNA cloning and nucleotide sequencing of a splitting storage protein subunit of soybean." Momma T., Negoro T., Hirano H., Matsumoto A., Udaka K., Fukazawa C. Eur. J. Biochem. 149:491-496(1985) [PubMed: 2988947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Bonminori.
[2]	"The primary structures of the A4 and A5 subunits are highly homologous to that of the A3 subunit in the glycinin seed storage protein of soybean." Hirano H., Fukazawa C., Harada K. FEBS Lett. 181:124-128(1985) Cited for: PROTEIN SEQUENCE (A4/A5 SUBUNITS). Strain: cv. Bonminori.
[3]	"Characterization of a null-allele for the Gy4 glycinin gene from soybean." Scallon B.J., Dickinson C.D., Nielsen N.C. Mol. Gen. Genet. 208:107-113(1987) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 181-386. Strain: cv. CX635-1-1-1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X02626 mRNA. Translation: CAA26478.1. X05652 mRNA. Translation: CAB57802.1.
PIR	FWSYG5. A91145. JA0152.
UniGene	Gma.29627. Gma.35585.
3D structure databases
ProteinModelPortal	P02858.
SMR	P02858. Positions 30-550.
ModBase	Search...
Protein family/group databases
Allergome	2541. Gly m 6.0401. 5821. Gly m 6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Gene expression databases
Genevestigator	P02858.
Family and domain databases
InterPro	IPR022379. 11S_seedstore_CS. IPR006044. 11S_seedstore_pln. IPR006045. Cupin_1. IPR011051. Cupin_RmlC_type. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 3 hits.
Pfam	PF00190. Cupin_1. 2 hits. [Graphical view]
PRINTS	PR00439. 11SGLOBULIN.
SMART	SM00835. Cupin_1. 2 hits. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
PROSITE	PS00305. 11S_SEED_STORAGE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYG4_SOYBN

Accession

Primary (citable) accession number: P02858

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	August 13, 1987
Last modified:	January 11, 2011
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program