Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P02833 (ANTP_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 153. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Homeotic protein antennapedia
Gene names
Name:Antp
ORF Names:CG1028
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length378 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sequence-specific transcription factor which is part of a developmental regulatory system that regulates segmental identity in the mesothorax. Provides cells with specific positional identities on the anterior-posterior axis.

Subcellular location

Nucleus Probable.

Miscellaneous

Loss of Antp results in altered development of the embryonic thoracic segments. Overexpression can cause antennae to be transformed into legs.

Sequence similarities

Belongs to the Antp homeobox family.

Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainHomeobox
Repeat
   LigandDNA-binding
   Molecular functionDevelopmental protein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processanterior/posterior axis specification

Inferred from mutant phenotype Ref.11. Source: UniProtKB

heart development

Inferred from expression pattern PubMed 12435360. Source: FlyBase

lymph gland development

Inferred from mutant phenotype PubMed 17361183. Source: FlyBase

midgut development

Traceable author statement PubMed 10512197. Source: FlyBase

muscle cell fate specification

Inferred from mutant phenotype PubMed 20056681. Source: FlyBase

neuroblast development

Inferred from mutant phenotype PubMed 18948419. Source: FlyBase

positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 11262224. Source: FlyBase

specification of segmental identity, antennal segment

Inferred from mutant phenotype PubMed 16299391. Source: FlyBase

specification of segmental identity, thorax

Inferred from mutant phenotype Ref.11. Source: UniProtKB

transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 11262224. Source: GOC

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 11262224. Source: FlyBase

nucleus

Inferred from direct assay Ref.14. Source: UniProtKB

   Molecular_functionDNA binding

Inferred from direct assay Ref.14. Source: UniProtKB

RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 11262224. Source: FlyBase

sequence-specific DNA binding

Inferred from direct assay PubMed 21575621. Source: FlyBase

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P02833-1)

Also known as: A; B; I; J;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P02833-2)

Also known as: H;

The sequence of this isoform differs from the canonical sequence as follows:
     296-297: ER → GK
     298-378: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 378378Homeotic protein antennapedia
PRO_0000200259

Regions

DNA binding297 – 35660Homeobox
Motif283 – 2886Antp-type hexapeptide
Compositional bias76 – 816Gln-rich (OPA repeat)
Compositional bias110 – 15546Gln-rich (OPA repeat)

Natural variations

Alternative sequence296 – 2972ER → GK in isoform 2.
VSP_008097
Alternative sequence298 – 37881Missing in isoform 2.
VSP_008098

Experimental info

Sequence conflict3001G → E in AAA79241. Ref.10

Secondary structure

......... 378
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (A) (B) (I) (J) [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: D653232A8622D055

FASTA37842,760
        10         20         30         40         50         60 
MTMSTNNCES MTSYFTNSYM GADMHHGHYP GNGVTDLDAQ QMHHYSQNAN HQGNMPYPRF 

        70         80         90        100        110        120 
PPYDRMPYYN GQGMDQQQQH QVYSRPDSPS SQVGGVMPQA QTNGQLGVPQ QQQQQQQQPS 

       130        140        150        160        170        180 
QNQQQQQAQQ APQQLQQQLP QVTQQVTHPQ QQQQQPVVYA SCKLQAAVGG LGMVPEGGSP 

       190        200        210        220        230        240 
PLVDQMSGHH MNAQMTLPHH MGHPQAQLGY TDVGVPDVTE VHQNHHNMGM YQQQSGVPPV 

       250        260        270        280        290        300 
GAPPQGMMHQ GQGPPQMHQG HPGQHTPPSQ NPNSQSSGMP SPLYPWMRSQ FGKCQERKRG 

       310        320        330        340        350        360 
RQTYTRYQTL ELEKEFHFNR YLTRRRRIEI AHALCLTERQ IKIWFQNRRM KWKKENKTKG 

       370 
EPGSGGEGDE ITPPNSPQ 

« Hide

Isoform 2 (H) [UniParc].

Checksum: A88C5435ABD619B3
Show »

FASTA29732,844

References

« Hide 'large scale' references
[1]"Structural organization and sequence of the homeotic gene Antennapedia of Drosophila melanogaster."
Schneuwly S., Kuroiwa A., Baumgartner P., Gehring W.J.
EMBO J. 5:733-739(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[2]"Multiple transcripts from the Antennapedia gene of Drosophila melanogaster."
Stroeher V.L., Jorgensen E.M., Garber R.L.
Mol. Cell. Biol. 6:4667-4675(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: Oregon-R.
Tissue: Embryo, Larva and Pupae.
[3]"Structure of transcripts from the homeotic Antennapedia gene of Drosophila melanogaster: two promoters control the major protein-coding region."
Laughon A., Boulet A.M., Bermingham J.R. Jr., Laymon R.A., Scott M.P.
Mol. Cell. Biol. 6:4676-4689(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[4]"Complete sequence of the Antennapedia complex of Drosophila."
Celniker S.E., Pfeiffer B., Knafels J., Martin C.H., Mayeda C.A., Palazzolo M.J.
Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[5]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[6]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[7]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: Berkeley.
Tissue: Embryo.
[8]"Structural relationships among genes that control development: sequence homology between the Antennapedia, Ultrabithorax, and fushi tarazu loci of Drosophila."
Scott M.P., Weiner A.J.
Proc. Natl. Acad. Sci. U.S.A. 81:4115-4119(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 296-364.
[9]"A homologous protein-coding sequence in Drosophila homeotic genes and its conservation in other metazoans."
McGinnis W., Garber R.L., Wirz J., Kuroiwa A., Gehring W.J.
Cell 37:403-408(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 296-378.
[10]"Homeo box genes of the Antennapedia and bithorax complexes of Drosophila."
Regulski M., Harding K., Kostriken R., Karch F., Levine M., McGinnis W.
Cell 43:71-80(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 297-357.
[11]"Redesigning the body plan of Drosophila by ectopic expression of the homoeotic gene Antennapedia."
Schneuwly S., Klemenz R., Gehring W.J.
Nature 325:816-818(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTANT ANALYSIS.
[12]"Determination of the three-dimensional structure of the Antennapedia homeodomain from Drosophila in solution by 1H nuclear magnetic resonance spectroscopy."
Billeter M., Qian Y.-Q., Otting G., Mueller M., Gehring W.J., Wuethrich K.
J. Mol. Biol. 214:183-197(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF HOMEOBOX.
[13]"Nuclear magnetic resonance spectroscopy of a DNA complex with the uniformly 13C-labeled Antennapedia homeodomain and structure determination of the DNA-bound homeodomain."
Qian Y.-Q., Otting G., Billeter M., Mueller M., Gehring W.J., Wuethrich K.
J. Mol. Biol. 234:1070-1083(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF HOMEOBOX.
[14]"Determination of the nuclear magnetic resonance solution structure of an Antennapedia homeodomain-DNA complex."
Billeter M., Qian Y.-Q., Otting G., Mueller M., Gehring W.J., Wuethrich K.
J. Mol. Biol. 234:1084-1093(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF HOMEOBOX.
[15]"NMR structure determination reveals that the homeodomain is connected through a flexible linker to the main body in the Drosophila Antennapedia protein."
Qian Y.-Q., Otting G., Furukubo-Tokunaga K., Affolter M., Gehring W.J., Wuethrich K.
Proc. Natl. Acad. Sci. U.S.A. 89:10738-10742(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 279-363.
[16]"Comparison of X-ray and NMR structures for the Antennapedia homeodomain-DNA complex."
Fraenkel E., Pabo C.O.
Nat. Struct. Biol. 5:692-697(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 297-356.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X03790, X03791 Genomic DNA. Translation: CAA27417.1.
M20704 mRNA. Translation: AAA70214.1.
M20705 mRNA. Translation: AAA70216.1.
AE001572 Genomic DNA. Translation: AAD19793.1.
AE014297 Genomic DNA. Translation: AAG22205.3.
AE014297 Genomic DNA. Translation: AAS65111.1.
AY060407 mRNA. Translation: AAL25446.1.
M14496 Genomic DNA. Translation: AAA28376.1.
K01948 Genomic DNA. Translation: AAA28373.1. Sequence problems.
M12009 mRNA. Translation: AAA79241.1.
PIRA25399. A23450.
RefSeqNP_996167.1. NM_206445.1.
NP_996168.1. NM_206446.1.
NP_996170.1. NM_206448.1.
NP_996175.1. NM_206453.1.
NP_996176.1. NM_206454.1.
UniGeneDm.3803.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AHDNMR-P297-363[»]
1HOMNMR-A297-363[»]
1KZ0NMR-A339-354[»]
1KZ5NMR-A339-351[»]
1OMQNMR-A339-354[»]
1SANNMR-A303-363[»]
2HOANMR-A297-363[»]
9ANTX-ray2.40A/B296-356[»]
ProteinModelPortalP02833.
SMRP02833. Positions 297-363.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid66033. 24 interactions.
DIPDIP-51405N.
IntActP02833. 2 interactions.
MINTMINT-763892.

Proteomic databases

PRIDEP02833.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0081647; FBpp0081161; FBgn0260642. [P02833-1]
FBtr0081652; FBpp0081162; FBgn0260642. [P02833-1]
FBtr0081654; FBpp0089245; FBgn0260642. [P02833-1]
FBtr0081655; FBpp0089246; FBgn0260642. [P02833-1]
GeneID40835.
KEGGdme:Dmel_CG1028.

Organism-specific databases

CTD40835.
FlyBaseFBgn0260642. Antp.

Phylogenomic databases

eggNOGNOG238660.
GeneTreeENSGT00730000110212.
HOGENOMHOG000142851.
InParanoidP02833.
KOK09311.
OMANCESMTS.
OrthoDBEOG780RP4.
PhylomeDBP02833.

Enzyme and pathway databases

SignaLinkP02833.

Gene expression databases

BgeeP02833.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR017995. Homeobox_antennapedia.
IPR001827. Homeobox_Antennapedia_CS.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSPR00025. ANTENNAPEDIA.
PR00024. HOMEOBOX.
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00032. ANTENNAPEDIA. 1 hit.
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP02833.
GenomeRNAi40835.
NextBio820851.

Entry information

Entry nameANTP_DROME
AccessionPrimary (citable) accession number: P02833
Secondary accession number(s): Q95SZ6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1988
Last modified: April 16, 2014
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Vertebrate homeotic Hox proteins

Nomenclature of vertebrate homeotic Hox proteins and list of entries

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase