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P02833

- ANTP_DROME

UniProt

P02833 - ANTP_DROME

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Protein

Homeotic protein antennapedia

Gene

Antp

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Sequence-specific transcription factor which is part of a developmental regulatory system that regulates segmental identity in the mesothorax. Provides cells with specific positional identities on the anterior-posterior axis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi297 – 35660HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB
  2. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: FlyBase
  3. sequence-specific DNA binding Source: FlyBase

GO - Biological processi

  1. anterior/posterior axis specification Source: UniProtKB
  2. heart development Source: FlyBase
  3. lymph gland development Source: FlyBase
  4. midgut development Source: FlyBase
  5. muscle cell fate specification Source: FlyBase
  6. neuroblast development Source: FlyBase
  7. positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  8. specification of segmental identity, antennal segment Source: FlyBase
  9. specification of segmental identity, thorax Source: UniProtKB
  10. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

SignaLinkiP02833.

Names & Taxonomyi

Protein namesi
Recommended name:
Homeotic protein antennapedia
Gene namesi
Name:Antp
ORF Names:CG1028
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0260642. Antp.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. cytoplasm Source: FlyBase
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 378378Homeotic protein antennapediaPRO_0000200259Add
BLAST

Proteomic databases

PRIDEiP02833.

Expressioni

Gene expression databases

BgeeiP02833.
ExpressionAtlasiP02833. differential.

Interactioni

Protein-protein interaction databases

BioGridi66033. 24 interactions.
DIPiDIP-51405N.
IntActiP02833. 2 interactions.
MINTiMINT-763892.

Structurei

Secondary structure

1
378
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi299 – 3013
Helixi306 – 31611
Helixi324 – 33411
Helixi338 – 35417

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AHDNMR-P297-363[»]
1HOMNMR-A297-363[»]
1KZ0NMR-A339-354[»]
1KZ5NMR-A339-354[»]
1OMQNMR-A339-354[»]
1SANNMR-A303-363[»]
2HOANMR-A297-363[»]
9ANTX-ray2.40A/B296-356[»]
ProteinModelPortaliP02833.
SMRiP02833. Positions 297-363.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02833.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi283 – 2886Antp-type hexapeptide

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi76 – 816Gln-rich (OPA repeat)
Compositional biasi110 – 15546Gln-rich (OPA repeat)Add
BLAST

Sequence similaritiesi

Belongs to the Antp homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox, Repeat

Phylogenomic databases

eggNOGiNOG238660.
GeneTreeiENSGT00760000118945.
HOGENOMiHOG000142851.
InParanoidiP02833.
KOiK09311.
OMAiNCESMTS.
OrthoDBiEOG780RP4.
PhylomeDBiP02833.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017995. Homeobox_antennapedia.
IPR001827. Homeobox_Antennapedia_CS.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00025. ANTENNAPEDIA.
PR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00032. ANTENNAPEDIA. 1 hit.
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P02833-1) [UniParc]FASTAAdd to Basket

Also known as: A, B, I, J

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTMSTNNCES MTSYFTNSYM GADMHHGHYP GNGVTDLDAQ QMHHYSQNAN
60 70 80 90 100
HQGNMPYPRF PPYDRMPYYN GQGMDQQQQH QVYSRPDSPS SQVGGVMPQA
110 120 130 140 150
QTNGQLGVPQ QQQQQQQQPS QNQQQQQAQQ APQQLQQQLP QVTQQVTHPQ
160 170 180 190 200
QQQQQPVVYA SCKLQAAVGG LGMVPEGGSP PLVDQMSGHH MNAQMTLPHH
210 220 230 240 250
MGHPQAQLGY TDVGVPDVTE VHQNHHNMGM YQQQSGVPPV GAPPQGMMHQ
260 270 280 290 300
GQGPPQMHQG HPGQHTPPSQ NPNSQSSGMP SPLYPWMRSQ FGKCQERKRG
310 320 330 340 350
RQTYTRYQTL ELEKEFHFNR YLTRRRRIEI AHALCLTERQ IKIWFQNRRM
360 370
KWKKENKTKG EPGSGGEGDE ITPPNSPQ
Length:378
Mass (Da):42,760
Last modified:November 1, 1988 - v1
Checksum:iD653232A8622D055
GO
Isoform 2 (identifier: P02833-2) [UniParc]FASTAAdd to Basket

Also known as: H

The sequence of this isoform differs from the canonical sequence as follows:
     296-297: ER → GK
     298-378: Missing.

Note: No experimental confirmation available.

Show »
Length:297
Mass (Da):32,844
Checksum:iA88C5435ABD619B3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti300 – 3001G → E in AAA79241. (PubMed:2416463)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei296 – 2972ER → GK in isoform 2. 1 PublicationVSP_008097
Alternative sequencei298 – 37881Missing in isoform 2. 1 PublicationVSP_008098Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03790, X03791 Genomic DNA. Translation: CAA27417.1.
M20704 mRNA. Translation: AAA70214.1.
M20705 mRNA. Translation: AAA70216.1.
AE001572 Genomic DNA. Translation: AAD19793.1.
AE014297 Genomic DNA. Translation: AAG22205.3.
AE014297 Genomic DNA. Translation: AAS65111.1.
AY060407 mRNA. Translation: AAL25446.1.
M14496 Genomic DNA. Translation: AAA28376.1.
K01948 Genomic DNA. Translation: AAA28373.1. Sequence problems.
M12009 mRNA. Translation: AAA79241.1.
PIRiA23450. A25399.
RefSeqiNP_996167.1. NM_206445.1. [P02833-1]
NP_996168.1. NM_206446.1. [P02833-1]
NP_996170.1. NM_206448.1. [P02833-1]
NP_996175.1. NM_206453.1. [P02833-1]
NP_996176.1. NM_206454.1. [P02833-2]
UniGeneiDm.3803.

Genome annotation databases

EnsemblMetazoaiFBtr0081647; FBpp0081161; FBgn0260642. [P02833-1]
FBtr0081652; FBpp0081162; FBgn0260642. [P02833-1]
FBtr0081654; FBpp0089245; FBgn0260642. [P02833-1]
FBtr0081655; FBpp0089246; FBgn0260642. [P02833-1]
GeneIDi40835.
KEGGidme:Dmel_CG1028.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03790 , X03791 Genomic DNA. Translation: CAA27417.1 .
M20704 mRNA. Translation: AAA70214.1 .
M20705 mRNA. Translation: AAA70216.1 .
AE001572 Genomic DNA. Translation: AAD19793.1 .
AE014297 Genomic DNA. Translation: AAG22205.3 .
AE014297 Genomic DNA. Translation: AAS65111.1 .
AY060407 mRNA. Translation: AAL25446.1 .
M14496 Genomic DNA. Translation: AAA28376.1 .
K01948 Genomic DNA. Translation: AAA28373.1 . Sequence problems.
M12009 mRNA. Translation: AAA79241.1 .
PIRi A23450. A25399.
RefSeqi NP_996167.1. NM_206445.1. [P02833-1 ]
NP_996168.1. NM_206446.1. [P02833-1 ]
NP_996170.1. NM_206448.1. [P02833-1 ]
NP_996175.1. NM_206453.1. [P02833-1 ]
NP_996176.1. NM_206454.1. [P02833-2 ]
UniGenei Dm.3803.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1AHD NMR - P 297-363 [» ]
1HOM NMR - A 297-363 [» ]
1KZ0 NMR - A 339-354 [» ]
1KZ5 NMR - A 339-354 [» ]
1OMQ NMR - A 339-354 [» ]
1SAN NMR - A 303-363 [» ]
2HOA NMR - A 297-363 [» ]
9ANT X-ray 2.40 A/B 296-356 [» ]
ProteinModelPortali P02833.
SMRi P02833. Positions 297-363.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 66033. 24 interactions.
DIPi DIP-51405N.
IntActi P02833. 2 interactions.
MINTi MINT-763892.

Proteomic databases

PRIDEi P02833.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0081647 ; FBpp0081161 ; FBgn0260642 . [P02833-1 ]
FBtr0081652 ; FBpp0081162 ; FBgn0260642 . [P02833-1 ]
FBtr0081654 ; FBpp0089245 ; FBgn0260642 . [P02833-1 ]
FBtr0081655 ; FBpp0089246 ; FBgn0260642 . [P02833-1 ]
GeneIDi 40835.
KEGGi dme:Dmel_CG1028.

Organism-specific databases

CTDi 40835.
FlyBasei FBgn0260642. Antp.

Phylogenomic databases

eggNOGi NOG238660.
GeneTreei ENSGT00760000118945.
HOGENOMi HOG000142851.
InParanoidi P02833.
KOi K09311.
OMAi NCESMTS.
OrthoDBi EOG780RP4.
PhylomeDBi P02833.

Enzyme and pathway databases

SignaLinki P02833.

Miscellaneous databases

EvolutionaryTracei P02833.
GenomeRNAii 40835.
NextBioi 820851.

Gene expression databases

Bgeei P02833.
ExpressionAtlasi P02833. differential.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
InterProi IPR017995. Homeobox_antennapedia.
IPR001827. Homeobox_Antennapedia_CS.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view ]
Pfami PF00046. Homeobox. 1 hit.
[Graphical view ]
PRINTSi PR00025. ANTENNAPEDIA.
PR00024. HOMEOBOX.
SMARTi SM00389. HOX. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
PROSITEi PS00032. ANTENNAPEDIA. 1 hit.
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural organization and sequence of the homeotic gene Antennapedia of Drosophila melanogaster."
    Schneuwly S., Kuroiwa A., Baumgartner P., Gehring W.J.
    EMBO J. 5:733-739(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
  2. "Multiple transcripts from the Antennapedia gene of Drosophila melanogaster."
    Stroeher V.L., Jorgensen E.M., Garber R.L.
    Mol. Cell. Biol. 6:4667-4675(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Oregon-R.
    Tissue: Embryo, Larva and Pupae.
  3. "Structure of transcripts from the homeotic Antennapedia gene of Drosophila melanogaster: two promoters control the major protein-coding region."
    Laughon A., Boulet A.M., Bermingham J.R. Jr., Laymon R.A., Scott M.P.
    Mol. Cell. Biol. 6:4676-4689(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
  4. "Complete sequence of the Antennapedia complex of Drosophila."
    Celniker S.E., Pfeiffer B., Knafels J., Martin C.H., Mayeda C.A., Palazzolo M.J.
    Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
  5. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  6. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: Berkeley.
    Tissue: Embryo.
  8. "Structural relationships among genes that control development: sequence homology between the Antennapedia, Ultrabithorax, and fushi tarazu loci of Drosophila."
    Scott M.P., Weiner A.J.
    Proc. Natl. Acad. Sci. U.S.A. 81:4115-4119(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 296-364.
  9. "A homologous protein-coding sequence in Drosophila homeotic genes and its conservation in other metazoans."
    McGinnis W., Garber R.L., Wirz J., Kuroiwa A., Gehring W.J.
    Cell 37:403-408(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 296-378.
  10. "Homeo box genes of the Antennapedia and bithorax complexes of Drosophila."
    Regulski M., Harding K., Kostriken R., Karch F., Levine M., McGinnis W.
    Cell 43:71-80(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 297-357.
  11. "Redesigning the body plan of Drosophila by ectopic expression of the homoeotic gene Antennapedia."
    Schneuwly S., Klemenz R., Gehring W.J.
    Nature 325:816-818(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTANT ANALYSIS.
  12. "Determination of the three-dimensional structure of the Antennapedia homeodomain from Drosophila in solution by 1H nuclear magnetic resonance spectroscopy."
    Billeter M., Qian Y.-Q., Otting G., Mueller M., Gehring W.J., Wuethrich K.
    J. Mol. Biol. 214:183-197(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF HOMEOBOX.
  13. "Nuclear magnetic resonance spectroscopy of a DNA complex with the uniformly 13C-labeled Antennapedia homeodomain and structure determination of the DNA-bound homeodomain."
    Qian Y.-Q., Otting G., Billeter M., Mueller M., Gehring W.J., Wuethrich K.
    J. Mol. Biol. 234:1070-1083(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF HOMEOBOX.
  14. "Determination of the nuclear magnetic resonance solution structure of an Antennapedia homeodomain-DNA complex."
    Billeter M., Qian Y.-Q., Otting G., Mueller M., Gehring W.J., Wuethrich K.
    J. Mol. Biol. 234:1084-1093(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF HOMEOBOX.
  15. "NMR structure determination reveals that the homeodomain is connected through a flexible linker to the main body in the Drosophila Antennapedia protein."
    Qian Y.-Q., Otting G., Furukubo-Tokunaga K., Affolter M., Gehring W.J., Wuethrich K.
    Proc. Natl. Acad. Sci. U.S.A. 89:10738-10742(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 279-363.
  16. "Comparison of X-ray and NMR structures for the Antennapedia homeodomain-DNA complex."
    Fraenkel E., Pabo C.O.
    Nat. Struct. Biol. 5:692-697(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 297-356.

Entry informationi

Entry nameiANTP_DROME
AccessioniPrimary (citable) accession number: P02833
Secondary accession number(s): Q95SZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1988
Last modified: October 29, 2014
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

Loss of Antp results in altered development of the embryonic thoracic segments. Overexpression can cause antennae to be transformed into legs.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Vertebrate homeotic Hox proteins
    Nomenclature of vertebrate homeotic Hox proteins and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3