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Protein

Heat shock protein 83

Gene

Hsp83

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Together with Hop and piwi, mediates canalization, also known as developmental robustness, likely via epigenetic silencing of existing genetic variants and suppression of transposon-induced new genetic variation. Required for piRNA biogenesis by facilitating loading of piRNAs into PIWI proteins.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391ATPBy similarity
Binding sitei81 – 811ATPBy similarity
Binding sitei100 – 1001ATPBy similarity
Binding sitei126 – 1261ATP; via amide nitrogenBy similarity
Binding sitei385 – 3851ATPBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • unfolded protein binding Source: FlyBase

GO - Biological processi

  • actin filament organization Source: FlyBase
  • anterior/posterior axis specification, embryo Source: FlyBase
  • centrosome cycle Source: FlyBase
  • cold acclimation Source: FlyBase
  • mitotic spindle organization Source: FlyBase
  • negative regulation of cell proliferation Source: FlyBase
  • negative regulation of transposition Source: FlyBase
  • oogenesis Source: FlyBase
  • pole plasm mRNA localization Source: FlyBase
  • proteasome assembly Source: FlyBase
  • protein folding Source: FlyBase
  • R7 cell fate commitment Source: FlyBase
  • regulation of cell shape Source: FlyBase
  • regulation of circadian sleep/wake cycle, sleep Source: FlyBase
  • response to heat Source: FlyBase
  • spermatogenesis Source: FlyBase
  • torso signaling pathway Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-1227986. Signaling by ERBB2.
R-DME-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
R-DME-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-DME-203615. eNOS activation.
R-DME-3371571. HSF1-dependent transactivation.
R-DME-3928663. EPHA-mediated growth cone collapse.
R-DME-399954. Sema3A PAK dependent Axon repulsion.
R-DME-4420097. VEGFA-VEGFR2 Pathway.
R-DME-5218920. VEGFR2 mediated vascular permeability.
R-DME-844456. The NLRP3 inflammasome.

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock protein 83
Alternative name(s):
HSP 82
Gene namesi
Name:Hsp83
Synonyms:Hsp82
ORF Names:CG1242
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0001233. Hsp83.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: FlyBase
  • cytoplasm Source: FlyBase
  • lipid particle Source: FlyBase
  • perinuclear region of cytoplasm Source: FlyBase
  • plasma membrane Source: FlyBase
  • polytene chromosome interband Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 717717Heat shock protein 83PRO_0000062930Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei219 – 2191Phosphoserine1 Publication
Modified residuei294 – 2941Phosphotyrosine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP02828.
PRIDEiP02828.

PTM databases

iPTMnetiP02828.

Expressioni

Inductioni

In contrast to other major heat shock proteins, this one is also expressed at normal growth temperatures. It is also developmentally expressed during oogenesis.

Gene expression databases

BgeeiFBgn0001233.
ExpressionAtlasiP02828. differential.
GenevisibleiP02828. DM.

Interactioni

Subunit structurei

Homodimer (By similarity). Forms a complex with Hop and piwi; probably Hop mediates the interaction between piwi and Hsp83. Interacts with shu.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Nelf-EP922042EBI-136814,EBI-194490

GO - Molecular functioni

  • unfolded protein binding Source: FlyBase

Protein-protein interaction databases

BioGridi63886. 45 interactions.
DIPiDIP-17366N.
IntActiP02828. 3 interactions.
MINTiMINT-839447.
STRINGi7227.FBpp0305095.

Structurei

3D structure databases

ProteinModelPortaliP02828.
SMRiP02828. Positions 6-684.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi713 – 7175TPR repeat-binding

Domaini

The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.By similarity

Sequence similaritiesi

Belongs to the heat shock protein 90 family.Curated

Phylogenomic databases

eggNOGiKOG0020. Eukaryota.
COG0326. LUCA.
GeneTreeiENSGT00840000129758.
HOGENOMiHOG000263872.
InParanoidiP02828.
KOiK04079.
OMAiRIHRMMK.
OrthoDBiEOG091G0270.
PhylomeDBiP02828.

Family and domain databases

Gene3Di3.30.565.10. 2 hits.
HAMAPiMF_00505. HSP90. 1 hit.
InterProiIPR003594. HATPase_C.
IPR019805. Heat_shock_protein_90_CS.
IPR001404. Hsp90_fam.
IPR020575. Hsp90_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view]
PANTHERiPTHR11528. PTHR11528. 1 hit.
PfamiPF02518. HATPase_c. 1 hit.
PF00183. HSP90. 1 hit.
[Graphical view]
PIRSFiPIRSF002583. Hsp90. 1 hit.
PRINTSiPR00775. HEATSHOCK90.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS00298. HSP90. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P02828-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEEAETFAF QAEIAQLMSL IINTFYSNKE IFLRELISNA SDALDKIRYE
60 70 80 90 100
SLTDPSKLDS GKELYIKLIP NKTAGTLTII DTGIGMTKSD LVNNLGTIAK
110 120 130 140 150
SGTKAFMEAL QAGADISMIG QFGVGFYSAY LVADKVTVTS KNNDDEQYVW
160 170 180 190 200
ESSAGGSFTV RADNSEPLGR GTKIVLYIKE DQTDYLEESK IKEIVNKHSQ
210 220 230 240 250
FIGYPIKLLV EKEREKEVSD DEADDEKKEG DEKKEMETDE PKIEDVGEDE
260 270 280 290 300
DADKKDKDAK KKKTIKEKYT EDEELNKTKP IWTRNPDDIS QEEYGEFYKS
310 320 330 340 350
LTNDWEDHLA VKHFSVEGQL EFRALLFIPR RTPFDLFENQ KKRNNIKLYV
360 370 380 390 400
RRVFIMDNCE DLIPEYLNFM KGVVDSEDLP LNISREMLQQ NKVLKVIRKN
410 420 430 440 450
LVKKTMELIE ELTEDKENYK KFYDQFSKNL KLGVHEDSNN RAKLADFLRF
460 470 480 490 500
HTSASGDDFC SLADYVSRMK DNQKHVYFIT GESKDQVSNS AFVERVKARG
510 520 530 540 550
FEVVYMTEPI DEYVIQHLKE YKGKQLVSVT KEGLELPEDE SEKKKREEDK
560 570 580 590 600
AKFESLCKLM KSILDNKVEK VVVSNRLVDS PCCIVTSQFG WSANMERIMK
610 620 630 640 650
AQALRDTATM GYMAGKKQLE INPDHPIVET LRQKADADKN DKAVKDLVIL
660 670 680 690 700
LFETSLLSSG FSLDSPQVHA SRIYRMIKLG LGIDEDEPMT TDDAQSAGDA
710
PSLVEDTEDA SHMEEVD
Length:717
Mass (Da):81,865
Last modified:August 13, 1987 - v1
Checksum:iA71B96B2FE1684E6
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti234 – 2341Missing in strain: MA-10.2, DPF-13, DPF-82.1, EM-10, 178.7, 709.6 and MA-4.4.
Natural varianti368 – 3681N → K in strain: DPF-2, DPF-30, DPF-62, MA-10.2, DPF-82.1, 178.7, DPF-13, MA-4.4, EM-10, VC-805, MA-4.2, DPF-46, DPF-77, VC-815 and 709.6.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03810 Genomic DNA. Translation: CAA27435.1.
X00065 Genomic DNA. Translation: CAA24938.1.
AE014296 Genomic DNA. Translation: AAF47734.1.
AY122080 mRNA. Translation: AAM52592.1.
U57459 Genomic DNA. Translation: AAB46677.1.
U57460 Genomic DNA. Translation: AAB46678.1.
U57461 Genomic DNA. Translation: AAB46679.1.
U57462 Genomic DNA. Translation: AAB46680.1.
U57463 Genomic DNA. Translation: AAB46681.1.
U57464 Genomic DNA. Translation: AAB46682.1.
U57465 Genomic DNA. Translation: AAB46683.1.
U57466 Genomic DNA. Translation: AAB46684.1.
U57467 Genomic DNA. Translation: AAB46685.1.
U57468 Genomic DNA. Translation: AAB46686.1.
U57469 Genomic DNA. Translation: AAB46687.1.
U57470 Genomic DNA. Translation: AAB46688.1.
U57471 Genomic DNA. Translation: AAB46689.1.
U57472 Genomic DNA. Translation: AAB46690.1.
U57473 Genomic DNA. Translation: AAB46691.1.
PIRiA24827. HHFF83.
RefSeqiNP_001261362.1. NM_001274433.1.
NP_523899.1. NM_079175.4.
UniGeneiDm.2800.

Genome annotation databases

EnsemblMetazoaiFBtr0073040; FBpp0072904; FBgn0001233.
FBtr0332873; FBpp0305095; FBgn0001233.
GeneIDi38389.
KEGGidme:Dmel_CG1242.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03810 Genomic DNA. Translation: CAA27435.1.
X00065 Genomic DNA. Translation: CAA24938.1.
AE014296 Genomic DNA. Translation: AAF47734.1.
AY122080 mRNA. Translation: AAM52592.1.
U57459 Genomic DNA. Translation: AAB46677.1.
U57460 Genomic DNA. Translation: AAB46678.1.
U57461 Genomic DNA. Translation: AAB46679.1.
U57462 Genomic DNA. Translation: AAB46680.1.
U57463 Genomic DNA. Translation: AAB46681.1.
U57464 Genomic DNA. Translation: AAB46682.1.
U57465 Genomic DNA. Translation: AAB46683.1.
U57466 Genomic DNA. Translation: AAB46684.1.
U57467 Genomic DNA. Translation: AAB46685.1.
U57468 Genomic DNA. Translation: AAB46686.1.
U57469 Genomic DNA. Translation: AAB46687.1.
U57470 Genomic DNA. Translation: AAB46688.1.
U57471 Genomic DNA. Translation: AAB46689.1.
U57472 Genomic DNA. Translation: AAB46690.1.
U57473 Genomic DNA. Translation: AAB46691.1.
PIRiA24827. HHFF83.
RefSeqiNP_001261362.1. NM_001274433.1.
NP_523899.1. NM_079175.4.
UniGeneiDm.2800.

3D structure databases

ProteinModelPortaliP02828.
SMRiP02828. Positions 6-684.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi63886. 45 interactions.
DIPiDIP-17366N.
IntActiP02828. 3 interactions.
MINTiMINT-839447.
STRINGi7227.FBpp0305095.

PTM databases

iPTMnetiP02828.

Proteomic databases

PaxDbiP02828.
PRIDEiP02828.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0073040; FBpp0072904; FBgn0001233.
FBtr0332873; FBpp0305095; FBgn0001233.
GeneIDi38389.
KEGGidme:Dmel_CG1242.

Organism-specific databases

CTDi38389.
FlyBaseiFBgn0001233. Hsp83.

Phylogenomic databases

eggNOGiKOG0020. Eukaryota.
COG0326. LUCA.
GeneTreeiENSGT00840000129758.
HOGENOMiHOG000263872.
InParanoidiP02828.
KOiK04079.
OMAiRIHRMMK.
OrthoDBiEOG091G0270.
PhylomeDBiP02828.

Enzyme and pathway databases

ReactomeiR-DME-1227986. Signaling by ERBB2.
R-DME-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
R-DME-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-DME-203615. eNOS activation.
R-DME-3371571. HSF1-dependent transactivation.
R-DME-3928663. EPHA-mediated growth cone collapse.
R-DME-399954. Sema3A PAK dependent Axon repulsion.
R-DME-4420097. VEGFA-VEGFR2 Pathway.
R-DME-5218920. VEGFR2 mediated vascular permeability.
R-DME-844456. The NLRP3 inflammasome.

Miscellaneous databases

ChiTaRSiHsp83. fly.
GenomeRNAii38389.
PROiP02828.

Gene expression databases

BgeeiFBgn0001233.
ExpressionAtlasiP02828. differential.
GenevisibleiP02828. DM.

Family and domain databases

Gene3Di3.30.565.10. 2 hits.
HAMAPiMF_00505. HSP90. 1 hit.
InterProiIPR003594. HATPase_C.
IPR019805. Heat_shock_protein_90_CS.
IPR001404. Hsp90_fam.
IPR020575. Hsp90_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view]
PANTHERiPTHR11528. PTHR11528. 1 hit.
PfamiPF02518. HATPase_c. 1 hit.
PF00183. HSP90. 1 hit.
[Graphical view]
PIRSFiPIRSF002583. Hsp90. 1 hit.
PRINTSiPR00775. HEATSHOCK90.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS00298. HSP90. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHSP83_DROME
AccessioniPrimary (citable) accession number: P02828
Secondary accession number(s): P92174, P92202, Q9VZT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 13, 1987
Last modified: September 7, 2016
This is version 169 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.