Reviewed, UniProtKB/Swiss-Prot P02803 (MT1_RAT)

Names and origin

Protein names

Recommended name:
    Metallothionein-1
      Short name=MT-1
Alternative name(s):
    Metallothionein-I
      Short name=MT-I

Gene names

Name:	Mt1
Synonyms:	Mt1a

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	61 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Domain	Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
Sequence similarities	Belongs to the metallothionein superfamily. Type 1 family.

Ontologies

Keywords
Ligand	Metal-binding Metal-thiolate cluster Zinc
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 61	61	Metallothionein-1		PRO_0000197221
Regions
	Region	1 – 29	29	Beta
	Region	30 – 61	32	Alpha
Sites
	Metal binding	5	1	Cluster B
	Metal binding	7	1	Cluster B
	Metal binding	13	1	Cluster B
	Metal binding	15	1	Cluster B
	Metal binding	19	1	Cluster B
	Metal binding	21	1	Cluster B
	Metal binding	24	1	Cluster B
	Metal binding	26	1	Cluster B
	Metal binding	29	1	Cluster B
	Metal binding	33	1	Cluster A
	Metal binding	34	1	Cluster A
	Metal binding	36	1	Cluster A
	Metal binding	37	1	Cluster A
	Metal binding	41	1	Cluster A
	Metal binding	44	1	Cluster A
	Metal binding	48	1	Cluster A
	Metal binding	50	1	Cluster A
	Metal binding	57	1	Cluster A
	Metal binding	59	1	Cluster A
	Metal binding	60	1	Cluster A
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P02803-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 4460B3720B9ABC18	FASTA	61	6,006
	10 20 30 40 50 60 MDPNCSCSTG GSCTCSSSCG CKNCKCTSCK KSCCSCCPVG CSKCAQGCVC KGASDKCTCC A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural characterization of the isoforms of neonatal and adult rat liver metallothionein." Winge D.R., Nielson K.B., Zeikus R.D., Gray W.R. J. Biol. Chem. 259:11419-11425(1984) [PubMed: 6470004] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Rat metallothionein-1 structural gene and three pseudogenes, one of which contains 5'-regulatory sequences." Andersen R.D., Birren B.W., Taplitz S.J., Herschman H.R. Mol. Cell. Biol. 6:302-314(1986) [PubMed: 3023830] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Molecular cloning of the rat metallothionein 1 (MT-1) mRNA sequence." Andersen R.D., Birren B.W., Ganz T., Piletz J.E., Herschman H.R. DNA 2:15-22(1983) [PubMed: 6687866] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[4]	"Rat metallothionein multigene family." Andersen R.D., Taplitz S.J., Birren B.W., Bristol G., Herschman H.R. Experientia Suppl. 52:373-384(1987) [PubMed: 2959527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
[6]	"Cloning of metallothionein cDNA from neonatal rat liver." Mercer J.F.B., Hudson P.J. Biosci. Rep. 2:761-768(1982) [PubMed: 6184083] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 44-61.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J00750 mRNA. Translation: AAA41590.1. M11794 Genomic DNA. Translation: AAA41641.1. BC058442 mRNA. Translation: AAH58442.1. M24327 mRNA. Translation: AAA41589.1.
IPI	IPI00192524.
PIR	SMRT1. A93079.
RefSeq	NP_620181.1.
UniGene	Rn.54397
3D structure databases
SMR	P02803. Positions 1-61.
ModBase	Search...
Protein-protein interaction databases
STRING	P02803.
PTM databases
PhosphoSite	P02803.
Genome annotation databases
Ensembl	ENSRNOT00000038212; ENSRNOP00000030623; ENSRNOG00000025764; Rattus norvegicus. [Genome view] ENSRNOT00000057898; ENSRNOP00000054706; ENSRNOG00000038047; Rattus norvegicus. [Genome view]
GeneID	24567.
KEGG	rno:24567.
UCSC	AY341880. rat.
Organism-specific databases
CTD	24567.
RGD	3117. Mt1a.
Phylogenomic databases
HOVERGEN	P02803.
OMA	NCNCANG
Gene expression databases
Genevestigator	P02803.
GermOnline	ENSRNOG00000038047. Rattus norvegicus.
Family and domain databases
InterPro	IPR017854. Metallothionein_sfam. IPR003019. Metallothionein_sfam_euk. IPR000006. Metallothionein_vert. IPR018064. Metallothionein_vert_metal_BS. [Graphical view]
Gene3D	G3DSA:4.10.10.10. Metallothionein_vert. 1 hit.
PANTHER	PTHR23299. Metallothionein_vert. 1 hit.
Pfam	PF00131. Metallothio. 1 hit. [Graphical view]
PRINTS	PR00860. MTVERTEBRATE.
PROSITE	PS00203. METALLOTHIONEIN_VRT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	603706.

Entry information

Entry name

MT1_RAT

Accession

Primary (citable) accession number: P02803

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	November 24, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)