Reviewed,
UniProtKB/Swiss-Prot P02803 (MT1_RAT)
Last modified
November 24, 2009.
Version 73.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Metallothionein-1 Short name=MT-1 Alternative name(s): Metallothionein-I Short name=MT-I | ||||
| Gene names |
| ||||
| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 61 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids. |
| Domain | Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines. |
| Sequence similarities | Belongs to the metallothionein superfamily. Type 1 family. |
Ontologies
| Keywords | |
|---|---|
| Ligand | Metal-binding Metal-thiolate cluster Zinc |
| PTM | Acetylation |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Molecular function | zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 61 | 61 | Metallothionein-1 | PRO_0000197221 | |||||
Regions | |||||||||
| Region | 1 – 29 | 29 | Beta | ||||||
| Region | 30 – 61 | 32 | Alpha | ||||||
Sites | |||||||||
| Metal binding | 5 | 1 | Cluster B | ||||||
| Metal binding | 7 | 1 | Cluster B | ||||||
| Metal binding | 13 | 1 | Cluster B | ||||||
| Metal binding | 15 | 1 | Cluster B | ||||||
| Metal binding | 19 | 1 | Cluster B | ||||||
| Metal binding | 21 | 1 | Cluster B | ||||||
| Metal binding | 24 | 1 | Cluster B | ||||||
| Metal binding | 26 | 1 | Cluster B | ||||||
| Metal binding | 29 | 1 | Cluster B | ||||||
| Metal binding | 33 | 1 | Cluster A | ||||||
| Metal binding | 34 | 1 | Cluster A | ||||||
| Metal binding | 36 | 1 | Cluster A | ||||||
| Metal binding | 37 | 1 | Cluster A | ||||||
| Metal binding | 41 | 1 | Cluster A | ||||||
| Metal binding | 44 | 1 | Cluster A | ||||||
| Metal binding | 48 | 1 | Cluster A | ||||||
| Metal binding | 50 | 1 | Cluster A | ||||||
| Metal binding | 57 | 1 | Cluster A | ||||||
| Metal binding | 59 | 1 | Cluster A | ||||||
| Metal binding | 60 | 1 | Cluster A | ||||||
Amino acid modifications | |||||||||
| Modified residue | 1 | 1 | N-acetylmethionine Ref.1 | ||||||
Sequences
References
| « Hide 'large scale' references | |
| [1] | "Structural characterization of the isoforms of neonatal and adult rat liver metallothionein." Winge D.R., Nielson K.B., Zeikus R.D., Gray W.R. J. Biol. Chem. 259:11419-11425(1984) [PubMed: 6470004] [Abstract] Cited for: PROTEIN SEQUENCE. |
| [2] | "Rat metallothionein-1 structural gene and three pseudogenes, one of which contains 5'-regulatory sequences." Andersen R.D., Birren B.W., Taplitz S.J., Herschman H.R. Mol. Cell. Biol. 6:302-314(1986) [PubMed: 3023830] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "Molecular cloning of the rat metallothionein 1 (MT-1) mRNA sequence." Andersen R.D., Birren B.W., Ganz T., Piletz J.E., Herschman H.R. DNA 2:15-22(1983) [PubMed: 6687866] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver. |
| [4] | "Rat metallothionein multigene family." Andersen R.D., Taplitz S.J., Birren B.W., Bristol G., Herschman H.R. Experientia Suppl. 52:373-384(1987) [PubMed: 2959527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary. |
| [6] | "Cloning of metallothionein cDNA from neonatal rat liver." Mercer J.F.B., Hudson P.J. Biosci. Rep. 2:761-768(1982) [PubMed: 6184083] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 44-61. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| J00750 mRNA. Translation: AAA41590.1. M11794 Genomic DNA. Translation: AAA41641.1. BC058442 mRNA. Translation: AAH58442.1. M24327 mRNA. Translation: AAA41589.1. | |
| IPI | IPI00192524. |
| PIR | SMRT1. A93079. |
| RefSeq | NP_620181.1. |
| UniGene | Rn.54397 |
3D structure databases | |
| SMR | P02803. Positions 1-61. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P02803. |
PTM databases | |
| PhosphoSite | P02803. |
Genome annotation databases | |
| Ensembl | ENSRNOT00000038212; ENSRNOP00000030623; ENSRNOG00000025764; Rattus norvegicus. [Genome view] ENSRNOT00000057898; ENSRNOP00000054706; ENSRNOG00000038047; Rattus norvegicus. [Genome view] |
| GeneID | 24567. |
| KEGG | rno:24567. |
| UCSC | AY341880. rat. |
Organism-specific databases | |
| CTD | 24567. |
| RGD | 3117. Mt1a. |
Phylogenomic databases | |
| HOVERGEN | P02803. |
| OMA | NCNCANG |
Gene expression databases | |
| Genevestigator | P02803. |
| GermOnline | ENSRNOG00000038047. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR017854. Metallothionein_sfam. IPR003019. Metallothionein_sfam_euk. IPR000006. Metallothionein_vert. IPR018064. Metallothionein_vert_metal_BS. [Graphical view] |
| Gene3D | G3DSA:4.10.10.10. Metallothionein_vert. 1 hit. |
| PANTHER | PTHR23299. Metallothionein_vert. 1 hit. |
| Pfam | PF00131. Metallothio. 1 hit. [Graphical view] |
| PRINTS | PR00860. MTVERTEBRATE. |
| PROSITE | PS00203. METALLOTHIONEIN_VRT. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 603706. |
Entry information
| Entry name | MT1_RAT | ||||||||
| Accession | Primary (citable) accession number: P02803 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Metallothioneins Classification of metallothioneins and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


