P02803 (MT1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metallothionein-1
Short name=MT-1

Alternative name(s):
Metallothionein-I
Short name=MT-I

Gene names

Name:	Mt1
Synonyms:	Mt1a

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	61 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Domain	Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
Sequence similarities	Belongs to the metallothionein superfamily. Type 1 family.

Ontologies

Keywords
Ligand	Metal-binding Metal-thiolate cluster Zinc
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of growth Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	zinc ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 61	61	Metallothionein-1		PRO_0000197221
Regions
	Region	1 – 29	29	Beta
	Region	30 – 61	32	Alpha
Sites
	Metal binding	5	1	Divalent metal cation; cluster B
	Metal binding	7	1	Divalent metal cation; cluster B
	Metal binding	13	1	Divalent metal cation; cluster B
	Metal binding	15	1	Divalent metal cation; cluster B
	Metal binding	19	1	Divalent metal cation; cluster B
	Metal binding	21	1	Divalent metal cation; cluster B
	Metal binding	24	1	Divalent metal cation; cluster B
	Metal binding	26	1	Divalent metal cation; cluster B
	Metal binding	29	1	Divalent metal cation; cluster B
	Metal binding	33	1	Divalent metal cation; cluster A
	Metal binding	34	1	Divalent metal cation; cluster A
	Metal binding	36	1	Divalent metal cation; cluster A
	Metal binding	37	1	Divalent metal cation; cluster A
	Metal binding	41	1	Divalent metal cation; cluster A
	Metal binding	44	1	Divalent metal cation; cluster A
	Metal binding	48	1	Divalent metal cation; cluster A
	Metal binding	50	1	Divalent metal cation; cluster A
	Metal binding	57	1	Divalent metal cation; cluster A
	Metal binding	59	1	Divalent metal cation; cluster A
	Metal binding	60	1	Divalent metal cation; cluster A
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P02803 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 4460B3720B9ABC18	FASTA	61	6,006
	10 20 30 40 50 60 MDPNCSCSTG GSCTCSSSCG CKNCKCTSCK KSCCSCCPVG CSKCAQGCVC KGASDKCTCC A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural characterization of the isoforms of neonatal and adult rat liver metallothionein." Winge D.R., Nielson K.B., Zeikus R.D., Gray W.R. J. Biol. Chem. 259:11419-11425(1984) [PubMed: 6470004] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Rat metallothionein-1 structural gene and three pseudogenes, one of which contains 5'-regulatory sequences." Andersen R.D., Birren B.W., Taplitz S.J., Herschman H.R. Mol. Cell. Biol. 6:302-314(1986) [PubMed: 3023830] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Molecular cloning of the rat metallothionein 1 (MT-1) mRNA sequence." Andersen R.D., Birren B.W., Ganz T., Piletz J.E., Herschman H.R. DNA 2:15-22(1983) [PubMed: 6687866] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[4]	"Rat metallothionein multigene family." Andersen R.D., Taplitz S.J., Birren B.W., Bristol G., Herschman H.R. Experientia Suppl. 52:373-384(1987) [PubMed: 2959527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
[6]	"Cloning of metallothionein cDNA from neonatal rat liver." Mercer J.F.B., Hudson P.J. Biosci. Rep. 2:761-768(1982) [PubMed: 6184083] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 44-61.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J00750 mRNA. Translation: AAA41590.1. M11794 Genomic DNA. Translation: AAA41641.1. BC058442 mRNA. Translation: AAH58442.1. M24327 mRNA. Translation: AAA41589.1.
IPI	IPI00192524.
PIR	SMRT1. A93079.
RefSeq	NP_620181.1. NM_138826.4.
UniGene	Rn.54397.
3D structure databases
ProteinModelPortal	P02803.
SMR	P02803. Positions 1-61.
ModBase	Search...
Protein-protein interaction databases
STRING	P02803.
PTM databases
PhosphoSite	P02803.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000038212; ENSRNOP00000030623; ENSRNOG00000025764. ENSRNOT00000057898; ENSRNOP00000054706; ENSRNOG00000038047.
GeneID	24567.
KEGG	rno:24567.
UCSC	AY341880. rat.
Organism-specific databases
CTD	4489.
RGD	3117. Mt1a.
Phylogenomic databases
eggNOG	maNOG21730.
HOVERGEN	HBG095667.
InParanoid	P02803.
OMA	SCKCKNC.
Gene expression databases
Genevestigator	P02803.
GermOnline	ENSRNOG00000038047. Rattus norvegicus.
Family and domain databases
InterPro	IPR017854. Metalthion_dom. IPR023587. Metalthion_dom_vert. IPR003019. Metalthion_sfam_euk. IPR000006. Metalthion_vert. IPR018064. Metalthion_vert_metal_BS. [Graphical view]
Gene3D	G3DSA:4.10.10.10. Metallothionein_vert. 1 hit.
KO	K14739.
PANTHER	PTHR23299. Metallothionein_vert. 1 hit.
Pfam	PF00131. Metallothio. 1 hit. [Graphical view]
PRINTS	PR00860. MTVERTEBRATE.
SUPFAM	SSF57868. Metallothionein_sfam. 1 hit.
PROSITE	PS00203. METALLOTHIONEIN_VRT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	603706.

Entry information

Entry name

MT1_RAT

Accession

Primary (citable) accession number: P02803

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	December 14, 2011
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program