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Protein

Metallothionein-1

Gene

Mt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi5 – 51Divalent metal cation; cluster B
Metal bindingi7 – 71Divalent metal cation; cluster B
Metal bindingi13 – 131Divalent metal cation; cluster B
Metal bindingi15 – 151Divalent metal cation; cluster B
Metal bindingi19 – 191Divalent metal cation; cluster B
Metal bindingi21 – 211Divalent metal cation; cluster B
Metal bindingi24 – 241Divalent metal cation; cluster B
Metal bindingi26 – 261Divalent metal cation; cluster B
Metal bindingi29 – 291Divalent metal cation; cluster B
Metal bindingi33 – 331Divalent metal cation; cluster A
Metal bindingi34 – 341Divalent metal cation; cluster A
Metal bindingi36 – 361Divalent metal cation; cluster A
Metal bindingi37 – 371Divalent metal cation; cluster A
Metal bindingi41 – 411Divalent metal cation; cluster A
Metal bindingi44 – 441Divalent metal cation; cluster A
Metal bindingi48 – 481Divalent metal cation; cluster A
Metal bindingi50 – 501Divalent metal cation; cluster A
Metal bindingi57 – 571Divalent metal cation; cluster A
Metal bindingi59 – 591Divalent metal cation; cluster A
Metal bindingi60 – 601Divalent metal cation; cluster A

GO - Molecular functioni

  • copper ion binding Source: MGI
  • metal ion binding Source: MGI
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • cellular metal ion homeostasis Source: MGI
  • cellular response to chromate Source: MGI
  • cellular response to zinc ion Source: MGI
  • cellular zinc ion homeostasis Source: MGI
  • detoxification of copper ion Source: MGI
  • negative regulation of growth Source: UniProtKB
  • negative regulation of neuron apoptotic process Source: UniProtKB
  • nitric oxide mediated signal transduction Source: MGI
  • response to metal ion Source: Reactome
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Metal-thiolate cluster

Enzyme and pathway databases

ReactomeiR-MMU-5661231. Metallothioneins bind metals.

Names & Taxonomyi

Protein namesi
Recommended name:
Metallothionein-1
Short name:
MT-1
Alternative name(s):
Metallothionein-I
Short name:
MT-I
Gene namesi
Name:Mt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:97171. Mt1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: MGI
  • lysosome Source: MGI
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6161Metallothionein-1PRO_0000197206Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP02802.
MaxQBiP02802.
PaxDbiP02802.
PeptideAtlasiP02802.
PRIDEiP02802.
TopDownProteomicsiP02802.

PTM databases

iPTMnetiP02802.
PhosphoSiteiP02802.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031765.
CleanExiMM_MT1.
GenevisibleiP02802. MM.

Interactioni

Protein-protein interaction databases

BioGridi201578. 1 interaction.
STRINGi10090.ENSMUSP00000034215.

Structurei

Secondary structure

1
61
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 113Combined sources
Beta strandi20 – 234Combined sources
Beta strandi25 – 284Combined sources
Beta strandi35 – 373Combined sources
Beta strandi42 – 476Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DFSNMR-A31-61[»]
1DFTNMR-A1-30[»]
ProteinModelPortaliP02802.
SMRiP02802. Positions 1-61.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02802.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2929BetaAdd
BLAST
Regioni30 – 6132AlphaAdd
BLAST

Domaini

Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410JFS1. Eukaryota.
ENOG41113QR. LUCA.
GeneTreeiENSGT00840000131018.
HOGENOMiHOG000236262.
HOVERGENiHBG009063.
InParanoidiP02802.
KOiK14739.
OMAiSCACKNC.
TreeFamiTF336054.

Family and domain databases

Gene3Di4.10.10.10. 1 hit.
InterProiIPR003019. Metalthion.
IPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR000006. Metalthion_vert.
IPR018064. Metalthion_vert_metal_BS.
[Graphical view]
PANTHERiPTHR23299. PTHR23299. 1 hit.
PfamiPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSiPR00860. MTVERTEBRATE.
SUPFAMiSSF57868. SSF57868. 1 hit.
PROSITEiPS00203. METALLOTHIONEIN_VRT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P02802-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPNCSCSTG GSCTCTSSCA CKNCKCTSCK KSCCSCCPVG CSKCAQGCVC
60
KGAADKCTCC A
Length:61
Mass (Da):6,018
Last modified:July 21, 1986 - v1
Checksum:i4B3754EC759C0ADB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti23 – 231N → D AA sequence (PubMed:914867).Curated
Sequence conflicti32 – 321S → G (PubMed:6277322).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S62785 mRNA. Translation: AAB26768.1.
J00605 Genomic DNA. Translation: AAA39527.2.
AK018727 mRNA. Translation: BAC25563.1.
BC036990 mRNA. Translation: AAH36990.1.
CCDSiCCDS40438.1.
PIRiA93261. SMMSI.
RefSeqiNP_038630.1. NM_013602.3.
UniGeneiMm.192991.
Mm.388249.

Genome annotation databases

EnsembliENSMUST00000034215; ENSMUSP00000034215; ENSMUSG00000031765.
GeneIDi17748.
KEGGimmu:17748.
UCSCiuc009mvw.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S62785 mRNA. Translation: AAB26768.1.
J00605 Genomic DNA. Translation: AAA39527.2.
AK018727 mRNA. Translation: BAC25563.1.
BC036990 mRNA. Translation: AAH36990.1.
CCDSiCCDS40438.1.
PIRiA93261. SMMSI.
RefSeqiNP_038630.1. NM_013602.3.
UniGeneiMm.192991.
Mm.388249.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DFSNMR-A31-61[»]
1DFTNMR-A1-30[»]
ProteinModelPortaliP02802.
SMRiP02802. Positions 1-61.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201578. 1 interaction.
STRINGi10090.ENSMUSP00000034215.

PTM databases

iPTMnetiP02802.
PhosphoSiteiP02802.

Proteomic databases

EPDiP02802.
MaxQBiP02802.
PaxDbiP02802.
PeptideAtlasiP02802.
PRIDEiP02802.
TopDownProteomicsiP02802.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034215; ENSMUSP00000034215; ENSMUSG00000031765.
GeneIDi17748.
KEGGimmu:17748.
UCSCiuc009mvw.2. mouse.

Organism-specific databases

CTDi17748.
MGIiMGI:97171. Mt1.

Phylogenomic databases

eggNOGiENOG410JFS1. Eukaryota.
ENOG41113QR. LUCA.
GeneTreeiENSGT00840000131018.
HOGENOMiHOG000236262.
HOVERGENiHBG009063.
InParanoidiP02802.
KOiK14739.
OMAiSCACKNC.
TreeFamiTF336054.

Enzyme and pathway databases

ReactomeiR-MMU-5661231. Metallothioneins bind metals.

Miscellaneous databases

EvolutionaryTraceiP02802.
PROiP02802.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031765.
CleanExiMM_MT1.
GenevisibleiP02802. MM.

Family and domain databases

Gene3Di4.10.10.10. 1 hit.
InterProiIPR003019. Metalthion.
IPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR000006. Metalthion_vert.
IPR018064. Metalthion_vert_metal_BS.
[Graphical view]
PANTHERiPTHR23299. PTHR23299. 1 hit.
PfamiPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSiPR00860. MTVERTEBRATE.
SUPFAMiSSF57868. SSF57868. 1 hit.
PROSITEiPS00203. METALLOTHIONEIN_VRT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMT1_MOUSE
AccessioniPrimary (citable) accession number: P02802
Secondary accession number(s): Q64485
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: September 7, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Metallothioneins
    Classification of metallothioneins and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.