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Protein

Metallothionein-1

Gene

Mt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi5Divalent metal cation; cluster B1
Metal bindingi7Divalent metal cation; cluster B1
Metal bindingi13Divalent metal cation; cluster B1
Metal bindingi15Divalent metal cation; cluster B1
Metal bindingi19Divalent metal cation; cluster B1
Metal bindingi21Divalent metal cation; cluster B1
Metal bindingi24Divalent metal cation; cluster B1
Metal bindingi26Divalent metal cation; cluster B1
Metal bindingi29Divalent metal cation; cluster B1
Metal bindingi33Divalent metal cation; cluster A1
Metal bindingi34Divalent metal cation; cluster A1
Metal bindingi36Divalent metal cation; cluster A1
Metal bindingi37Divalent metal cation; cluster A1
Metal bindingi41Divalent metal cation; cluster A1
Metal bindingi44Divalent metal cation; cluster A1
Metal bindingi48Divalent metal cation; cluster A1
Metal bindingi50Divalent metal cation; cluster A1
Metal bindingi57Divalent metal cation; cluster A1
Metal bindingi59Divalent metal cation; cluster A1
Metal bindingi60Divalent metal cation; cluster A1

GO - Molecular functioni

  • copper ion binding Source: MGI
  • metal ion binding Source: MGI
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • cellular metal ion homeostasis Source: MGI
  • cellular response to chromate Source: MGI
  • cellular response to zinc ion Source: MGI
  • cellular zinc ion homeostasis Source: MGI
  • detoxification of copper ion Source: MGI
  • negative regulation of growth Source: UniProtKB
  • negative regulation of neuron apoptotic process Source: UniProtKB
  • nitric oxide mediated signal transduction Source: MGI

Keywordsi

LigandMetal-binding, Metal-thiolate cluster

Names & Taxonomyi

Protein namesi
Recommended name:
Metallothionein-1
Short name:
MT-1
Alternative name(s):
Metallothionein-I
Short name:
MT-I
Gene namesi
Name:Mt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:97171 Mt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001972061 – 61Metallothionein-1Add BLAST61

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP02802
MaxQBiP02802
PaxDbiP02802
PeptideAtlasiP02802
PRIDEiP02802
TopDownProteomicsiP02802

PTM databases

iPTMnetiP02802
PhosphoSitePlusiP02802

Expressioni

Gene expression databases

BgeeiENSMUSG00000031765
CleanExiMM_MT1
ExpressionAtlasiP02802 baseline and differential
GenevisibleiP02802 MM

Interactioni

Protein-protein interaction databases

BioGridi201578, 1 interactor
STRINGi10090.ENSMUSP00000034215

Structurei

Secondary structure

161
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 11Combined sources3
Beta strandi20 – 23Combined sources4
Beta strandi25 – 28Combined sources4
Beta strandi35 – 37Combined sources3
Beta strandi42 – 47Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DFSNMR-A31-61[»]
1DFTNMR-A1-30[»]
ProteinModelPortaliP02802
SMRiP02802
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02802

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 29BetaAdd BLAST29
Regioni30 – 61AlphaAdd BLAST32

Domaini

Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410JFS1 Eukaryota
ENOG41113QR LUCA
GeneTreeiENSGT00730000110883
HOGENOMiHOG000236262
HOVERGENiHBG009063
InParanoidiP02802
KOiK14739
TreeFamiTF336054

Family and domain databases

Gene3Di4.10.10.10, 1 hit
InterProiView protein in InterPro
IPR003019 Metalthion
IPR017854 Metalthion_dom_sf
IPR023587 Metalthion_dom_sf_vert
IPR000006 Metalthion_vert
IPR018064 Metalthion_vert_metal_BS
PANTHERiPTHR23299 PTHR23299, 1 hit
PfamiView protein in Pfam
PF00131 Metallothio, 1 hit
PRINTSiPR00860 MTVERTEBRATE
SUPFAMiSSF57868 SSF57868, 1 hit
PROSITEiView protein in PROSITE
PS00203 METALLOTHIONEIN_VRT, 1 hit

Sequencei

Sequence statusi: Complete.

P02802-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPNCSCSTG GSCTCTSSCA CKNCKCTSCK KSCCSCCPVG CSKCAQGCVC
60
KGAADKCTCC A
Length:61
Mass (Da):6,018
Last modified:July 21, 1986 - v1
Checksum:i4B3754EC759C0ADB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti23N → D AA sequence (PubMed:914867).Curated1
Sequence conflicti32S → G (PubMed:6277322).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S62785 mRNA Translation: AAB26768.1
J00605 Genomic DNA Translation: AAA39527.2
AK018727 mRNA Translation: BAC25563.1
BC036990 mRNA Translation: AAH36990.1
CCDSiCCDS40438.1
PIRiA93261 SMMSI
RefSeqiNP_038630.1, NM_013602.3
UniGeneiMm.192991

Genome annotation databases

EnsembliENSMUST00000034215; ENSMUSP00000034215; ENSMUSG00000031765
GeneIDi17748
KEGGimmu:17748
UCSCiuc009mvw.2 mouse

Similar proteinsi

Entry informationi

Entry nameiMT1_MOUSE
AccessioniPrimary (citable) accession number: P02802
Secondary accession number(s): Q64485
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 25, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health