Reviewed, UniProtKB/Swiss-Prot P02801 (MT1B_HORSE)

Names and origin

Protein names	Recommended name:     Metallothionein-1B       Short name=MT-1B Alternative name(s):     Metallothionein-IB       Short name=MT-IB
Organism	Equus caballus (Horse)
Taxonomic identifier	9796 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus

Protein attributes

Sequence length	61 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Domain	Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
Sequence similarities	Belongs to the metallothionein superfamily. Type 1 family.

Ontologies

Keywords
Ligand	Metal-binding Metal-thiolate cluster
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 61	61	Metallothionein-1B		PRO_0000197205
Regions
	Region	1 – 29	29	Beta
	Region	30 – 61	32	Alpha
Sites
	Metal binding	5	1	Cluster B
	Metal binding	7	1	Cluster B
	Metal binding	13	1	Cluster B
	Metal binding	15	1	Cluster B
	Metal binding	19	1	Cluster B
	Metal binding	21	1	Cluster B
	Metal binding	24	1	Cluster B
	Metal binding	26	1	Cluster B
	Metal binding	29	1	Cluster B
	Metal binding	33	1	Cluster A
	Metal binding	34	1	Cluster A
	Metal binding	36	1	Cluster A
	Metal binding	37	1	Cluster A
	Metal binding	41	1	Cluster A
	Metal binding	44	1	Cluster A
	Metal binding	48	1	Cluster A
	Metal binding	50	1	Cluster A
	Metal binding	57	1	Cluster A
	Metal binding	59	1	Cluster A
	Metal binding	60	1	Cluster A
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine

Sequences

	Sequence		Length	Mass (Da)
	P02801-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 7919626F976005E3	FASTA	61	6,067
	10 20 30 40 50 60 MDPNCSCVAG ESCTCAGSCK CKQCRCASCK KSCCSCCPVG CAKCAQGCVC KGASDKCSCC A

References

[1]	"Amino-acid sequence of equine renal metallothionein-1B." Kojima Y., Berger C., Vallee B.L., Kaegi J.H.R. Proc. Natl. Acad. Sci. U.S.A. 73:3413-3417(1976) [PubMed: 1068454] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Kidney.
[2]	"Chemistry and biochemistry of metallothionein." Kaegi J.H.R., Kojima Y. Experientia Suppl. 52:25-61(1987) [PubMed: 2959513] [Abstract] Cited for: SEQUENCE REVISION TO 58-59.

Cross-references

Sequence databases
PIR	SMHOB. A03278.
3D structure databases
SMR	P02801. Positions 1-61.
ModBase	Search...
Genome annotation databases
Ensembl	ENSECAT00000015995; ENSECAP00000012898; ENSECAG00000015275; Equus caballus. [Genome view]
Phylogenomic databases
HOVERGEN	P02801.
InParanoid	P02801.
Family and domain databases
InterPro	IPR017854. Metallothionein_sfam. IPR003019. Metallothionein_sfam_euk. IPR000006. Metallothionein_vert. IPR018064. Metallothionein_vert_metal_BS. [Graphical view]
Gene3D	G3DSA:4.10.10.10. Metallothionein_vert. 1 hit.
PANTHER	PTHR23299. Metallothionein_vert. 1 hit.
Pfam	PF00131. Metallothio. 1 hit. [Graphical view]
PRINTS	PR00860. MTVERTEBRATE.
PROSITE	PS00203. METALLOTHIONEIN_VRT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MT1B_HORSE

Accession

Primary (citable) accession number: P02801

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	November 1, 1997
Last modified:	December 15, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)