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Protein

Metallothionein-2

Gene

Mt2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi5Divalent metal cation; cluster B1
Metal bindingi7Divalent metal cation; cluster B1
Metal bindingi13Divalent metal cation; cluster B1
Metal bindingi15Divalent metal cation; cluster B1
Metal bindingi19Divalent metal cation; cluster B1
Metal bindingi21Divalent metal cation; cluster B1
Metal bindingi24Divalent metal cation; cluster B1
Metal bindingi26Divalent metal cation; cluster B1
Metal bindingi29Divalent metal cation; cluster B1
Metal bindingi33Divalent metal cation; cluster A1
Metal bindingi34Divalent metal cation; cluster A1
Metal bindingi36Divalent metal cation; cluster A1
Metal bindingi37Divalent metal cation; cluster A1
Metal bindingi41Divalent metal cation; cluster A1
Metal bindingi44Divalent metal cation; cluster A1
Metal bindingi48Divalent metal cation; cluster A1
Metal bindingi50Divalent metal cation; cluster A1
Metal bindingi57Divalent metal cation; cluster A1
Metal bindingi59Divalent metal cation; cluster A1
Metal bindingi60Divalent metal cation; cluster A1

GO - Molecular functioni

GO - Biological processi

  • astrocyte activation Source: Ensembl
  • cellular response to drug Source: Ensembl
  • cellular response to erythropoietin Source: Ensembl
  • cellular response to interleukin-3 Source: Ensembl
  • cellular response to zinc ion Source: UniProtKB
  • cellular zinc ion homeostasis Source: MGI
  • detoxification of copper ion Source: MGI
  • negative regulation of growth Source: UniProtKB
  • nitric oxide mediated signal transduction Source: MGI
  • response to metal ion Source: Reactome

Keywordsi

LigandMetal-binding, Metal-thiolate cluster

Enzyme and pathway databases

ReactomeiR-MMU-5661231. Metallothioneins bind metals.

Names & Taxonomyi

Protein namesi
Recommended name:
Metallothionein-2
Short name:
MT-2
Alternative name(s):
Metallothionein-II
Short name:
MT-II
Gene namesi
Name:Mt2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:97172. Mt2.

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001972071 – 61Metallothionein-2Add BLAST61

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei58PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP02798.
MaxQBiP02798.
PaxDbiP02798.
PeptideAtlasiP02798.
PRIDEiP02798.

PTM databases

iPTMnetiP02798.
PhosphoSitePlusiP02798.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031762.
CleanExiMM_MT2.
ExpressionAtlasiP02798. baseline and differential.
GenevisibleiP02798. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034214.

Structurei

3D structure databases

ProteinModelPortaliP02798.
SMRiP02798.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 29BetaAdd BLAST29
Regioni30 – 61AlphaAdd BLAST32

Domaini

Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410JFS1. Eukaryota.
ENOG41113QR. LUCA.
GeneTreeiENSGT00730000110883.
HOGENOMiHOG000236262.
InParanoidiP02798.
KOiK14739.
OMAiCNCAGSC.
TreeFamiTF336054.

Family and domain databases

Gene3Di4.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR003019. Metalthion.
IPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR000006. Metalthion_vert.
IPR018064. Metalthion_vert_metal_BS.
PANTHERiPTHR23299. PTHR23299. 1 hit.
PfamiView protein in Pfam
PF00131. Metallothio. 1 hit.
PRINTSiPR00860. MTVERTEBRATE.
SUPFAMiSSF57868. SSF57868. 1 hit.
PROSITEiView protein in PROSITE
PS00203. METALLOTHIONEIN_VRT. 1 hit.

Sequencei

Sequence statusi: Complete.

P02798-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPNCSCASD GSCSCAGACK CKQCKCTSCK KSCCSCCPVG CAKCSQGCIC
60
KEASDKCSCC A
Length:61
Mass (Da):6,115
Last modified:August 1, 1991 - v2
Checksum:iA20D1311166672EC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti52E → Q AA sequence (PubMed:7287658).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02236 Genomic DNA. Translation: AAA39528.1.
AK002567 mRNA. Translation: BAB22192.1.
BC031758 mRNA. Translation: AAH31758.1.
CCDSiCCDS40437.1.
PIRiA03275. SMMS2.
I57572.
RefSeqiNP_032656.1. NM_008630.2.
UniGeneiMm.147226.

Genome annotation databases

EnsembliENSMUST00000034214; ENSMUSP00000034214; ENSMUSG00000031762.
GeneIDi17750.
KEGGimmu:17750.
UCSCiuc009mvv.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMT2_MOUSE
AccessioniPrimary (citable) accession number: P02798
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 1, 1991
Last modified: July 5, 2017
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Metallothioneins
    Classification of metallothioneins and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families