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P02795

- MT2_HUMAN

UniProt

P02795 - MT2_HUMAN

Protein

Metallothionein-2

Gene

MT2A

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi5 – 51Divalent metal cation; cluster B1 Publication
    Metal bindingi7 – 71Divalent metal cation; cluster B1 Publication
    Metal bindingi13 – 131Divalent metal cation; cluster B1 Publication
    Metal bindingi15 – 151Divalent metal cation; cluster B1 Publication
    Metal bindingi19 – 191Divalent metal cation; cluster B
    Metal bindingi21 – 211Divalent metal cation; cluster B
    Metal bindingi24 – 241Divalent metal cation; cluster B
    Metal bindingi26 – 261Divalent metal cation; cluster B
    Metal bindingi29 – 291Divalent metal cation; cluster B
    Metal bindingi33 – 331Divalent metal cation; cluster A
    Metal bindingi34 – 341Divalent metal cation; cluster A
    Metal bindingi36 – 361Divalent metal cation; cluster A
    Metal bindingi37 – 371Divalent metal cation; cluster A
    Metal bindingi41 – 411Divalent metal cation; cluster A
    Metal bindingi44 – 441Divalent metal cation; cluster A
    Metal bindingi48 – 481Divalent metal cation; cluster A
    Metal bindingi50 – 501Divalent metal cation; cluster A
    Metal bindingi57 – 571Divalent metal cation; cluster A
    Metal bindingi59 – 591Divalent metal cation; cluster A
    Metal bindingi60 – 601Divalent metal cation; cluster A

    GO - Molecular functioni

    1. drug binding Source: UniProtKB
    2. protein binding Source: IntAct
    3. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. cellular copper ion homeostasis Source: ProtInc
    2. cellular response to drug Source: UniProtKB
    3. cellular response to erythropoietin Source: UniProtKB
    4. cellular response to interleukin-3 Source: UniProtKB
    5. cellular response to zinc ion Source: UniProtKB
    6. cytokine-mediated signaling pathway Source: Reactome
    7. interferon-gamma-mediated signaling pathway Source: Reactome
    8. negative regulation of growth Source: UniProtKB

    Keywords - Ligandi

    Metal-binding, Metal-thiolate cluster, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_25078. Interferon gamma signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Metallothionein-2
    Short name:
    MT-2
    Alternative name(s):
    Metallothionein-2A
    Metallothionein-II
    Short name:
    MT-II
    Gene namesi
    Name:MT2A
    Synonyms:CES1, MT2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:7406. MT2A.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. cytosol Source: Reactome
    3. nucleus Source: UniProtKB
    4. perinuclear region of cytoplasm Source: UniProtKB

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA31214.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 6161Metallothionein-2PRO_0000197245Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine2 Publications

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP02795.
    PRIDEiP02795.

    PTM databases

    PhosphoSiteiP02795.

    Expressioni

    Gene expression databases

    ArrayExpressiP02795.
    BgeeiP02795.
    CleanExiHS_CES1.
    HS_MT2A.
    GenevestigatoriP02795.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CXorf40AQ8TE692EBI-996616,EBI-996609
    GPR50Q135852EBI-996616,EBI-8550965
    PRKD1Q151397EBI-996616,EBI-1181072
    SPINK7P580627EBI-996616,EBI-1182445

    Protein-protein interaction databases

    BioGridi110608. 13 interactions.
    DIPiDIP-35232N.
    IntActiP02795. 10 interactions.
    MINTiMINT-2836376.
    STRINGi9606.ENSP00000245185.

    Structurei

    Secondary structure

    1
    61
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 115
    Helixi27 – 293
    Beta strandi35 – 373
    Helixi42 – 443

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1MHUNMR-A31-61[»]
    2MHUNMR-A1-30[»]
    ProteinModelPortaliP02795.
    SMRiP02795. Positions 1-61.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP02795.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 2929BetaAdd
    BLAST
    Regioni30 – 6132AlphaAdd
    BLAST

    Domaini

    Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.

    Sequence similaritiesi

    Phylogenomic databases

    HOGENOMiHOG000236262.
    HOVERGENiHBG094456.
    InParanoidiP02795.
    KOiK14739.
    OMAiFLNVFAG.
    OrthoDBiEOG7PZS1X.
    PhylomeDBiP02795.
    TreeFamiTF336054.

    Family and domain databases

    Gene3Di4.10.10.10. 1 hit.
    InterProiIPR017854. Metalthion_dom.
    IPR023587. Metalthion_dom_vert.
    IPR003019. Metalthion_sfam_euk.
    IPR000006. Metalthion_vert.
    IPR018064. Metalthion_vert_metal_BS.
    [Graphical view]
    PANTHERiPTHR23299. PTHR23299. 1 hit.
    PfamiPF00131. Metallothio. 1 hit.
    [Graphical view]
    PRINTSiPR00860. MTVERTEBRATE.
    SUPFAMiSSF57868. SSF57868. 1 hit.
    PROSITEiPS00203. METALLOTHIONEIN_VRT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P02795-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDPNCSCAAG DSCTCAGSCK CKECKCTSCK KSCCSCCPVG CAKCAQGCIC   50
    KGASDKCSCC A 61
    Length:61
    Mass (Da):6,042
    Last modified:July 21, 1986 - v1
    Checksum:i7B0A7E1F99843078
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti42 – 421A → V.1 Publication
    Corresponds to variant rs35109646 [ dbSNP | Ensembl ].
    VAR_025840

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J00271 Genomic DNA. Translation: AAA59583.1.
    V00594 mRNA. Translation: CAA23841.1.
    M26637 mRNA. Translation: AAA59584.1. Sequence problems.
    S52379 mRNA. Translation: AAB24908.1.
    X97260 mRNA. Translation: CAA65915.1.
    BT007315 mRNA. Translation: AAP35979.1.
    DQ370420 Genomic DNA. Translation: ABC79300.1.
    BC007034 mRNA. Translation: AAH07034.1.
    BC070289 mRNA. Translation: AAH70289.1.
    CCDSiCCDS10763.1.
    PIRiA03271. SMHU2.
    I63131.
    RefSeqiNP_005944.1. NM_005953.3.
    UniGeneiHs.534330.

    Genome annotation databases

    EnsembliENST00000245185; ENSP00000245185; ENSG00000125148.
    GeneIDi4502.
    KEGGihsa:4502.
    UCSCiuc002ejh.3. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    NIEHS-SNPs

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J00271 Genomic DNA. Translation: AAA59583.1 .
    V00594 mRNA. Translation: CAA23841.1 .
    M26637 mRNA. Translation: AAA59584.1 . Sequence problems.
    S52379 mRNA. Translation: AAB24908.1 .
    X97260 mRNA. Translation: CAA65915.1 .
    BT007315 mRNA. Translation: AAP35979.1 .
    DQ370420 Genomic DNA. Translation: ABC79300.1 .
    BC007034 mRNA. Translation: AAH07034.1 .
    BC070289 mRNA. Translation: AAH70289.1 .
    CCDSi CCDS10763.1.
    PIRi A03271. SMHU2.
    I63131.
    RefSeqi NP_005944.1. NM_005953.3.
    UniGenei Hs.534330.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1MHU NMR - A 31-61 [» ]
    2MHU NMR - A 1-30 [» ]
    ProteinModelPortali P02795.
    SMRi P02795. Positions 1-61.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110608. 13 interactions.
    DIPi DIP-35232N.
    IntActi P02795. 10 interactions.
    MINTi MINT-2836376.
    STRINGi 9606.ENSP00000245185.

    PTM databases

    PhosphoSitei P02795.

    Proteomic databases

    MaxQBi P02795.
    PRIDEi P02795.

    Protocols and materials databases

    DNASUi 4502.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000245185 ; ENSP00000245185 ; ENSG00000125148 .
    GeneIDi 4502.
    KEGGi hsa:4502.
    UCSCi uc002ejh.3. human.

    Organism-specific databases

    CTDi 4502.
    GeneCardsi GC16P056642.
    HGNCi HGNC:7406. MT2A.
    MIMi 156360. gene.
    neXtProti NX_P02795.
    PharmGKBi PA31214.
    GenAtlasi Search...

    Phylogenomic databases

    HOGENOMi HOG000236262.
    HOVERGENi HBG094456.
    InParanoidi P02795.
    KOi K14739.
    OMAi FLNVFAG.
    OrthoDBi EOG7PZS1X.
    PhylomeDBi P02795.
    TreeFami TF336054.

    Enzyme and pathway databases

    Reactomei REACT_25078. Interferon gamma signaling.

    Miscellaneous databases

    ChiTaRSi MT2A. human.
    EvolutionaryTracei P02795.
    GeneWikii Metallothionein_2A.
    GenomeRNAii 4502.
    NextBioi 17406.
    PROi P02795.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P02795.
    Bgeei P02795.
    CleanExi HS_CES1.
    HS_MT2A.
    Genevestigatori P02795.

    Family and domain databases

    Gene3Di 4.10.10.10. 1 hit.
    InterProi IPR017854. Metalthion_dom.
    IPR023587. Metalthion_dom_vert.
    IPR003019. Metalthion_sfam_euk.
    IPR000006. Metalthion_vert.
    IPR018064. Metalthion_vert_metal_BS.
    [Graphical view ]
    PANTHERi PTHR23299. PTHR23299. 1 hit.
    Pfami PF00131. Metallothio. 1 hit.
    [Graphical view ]
    PRINTSi PR00860. MTVERTEBRATE.
    SUPFAMi SSF57868. SSF57868. 1 hit.
    PROSITEi PS00203. METALLOTHIONEIN_VRT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of human hepatic metallothionein."
      Kissling M.M., Kaegi J.H.R.
      FEBS Lett. 82:247-250(1977) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY PROTEIN SEQUENCE.
      Tissue: Liver.
    2. "Human metallothionein genes -- primary structure of the metallothionein-II gene and a related processed gene."
      Karin M., Richards R.I.
      Nature 299:797-802(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Human metallothionein genes: molecular cloning and sequence analysis of the mRNA."
      Karin M., Richards R.I.
      Nucleic Acids Res. 10:3165-3173(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "Changes in brain gene expression shared by scrapie and Alzheimer disease."
      Duguid J.R., Bohmont C.W., Liu N.G., Tourtellotte W.W.
      Proc. Natl. Acad. Sci. U.S.A. 86:7260-7264(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    5. "Expression of human metallothionein-II fusion protein in Escherichia coli."
      Yamazaki S., Nakanishi M., Hamamoto T., Hirata H., Ebihara A., Tokue A., Kagawa Y.
      Biochem. Int. 28:451-460(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    6. "Cloning and sequencing a novel metallothionein 1 isoform expressed in human reticulocytes."
      Lambert E., Kille P., Kay J., Swaminathan R.
      Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    7. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    8. NIEHS SNPs program
      Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-42.
    9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain and Kidney.
    10. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Three-dimensional structure of human [113Cd7]metallothionein-2 in solution determined by nuclear magnetic resonance spectroscopy."
      Messerle B.A., Schaeffer A., Vasak M., Kaegi J.H.R., Wuethrich K.
      J. Mol. Biol. 214:765-779(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    12. "Comparison of the solution conformations of human [Zn7]-metallothionein-2 and [Cd7]-metallothionein-2 using nuclear magnetic resonance spectroscopy."
      Messerle B.A., Schaeffer A., Vasak M., Kaegi J.H.R., Wuethrich K.
      J. Mol. Biol. 225:433-443(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.

    Entry informationi

    Entry nameiMT2_HUMAN
    AccessioniPrimary (citable) accession number: P02795
    Secondary accession number(s): Q14823, Q2HXR9, Q53XT9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 154 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    This metallothionein binds zinc.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. Metallothioneins
      Classification of metallothioneins and list of entries
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3