P02778 (CXL10_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-X-C motif chemokine 10

Alternative name(s):
10 kDa interferon gamma-induced protein
Short name=Gamma-IP10
Short name=IP-10
Small-inducible cytokine B10

Cleaved into the following chain:

1. CXCL10(1-73)

Gene names

Name:	CXCL10
Synonyms:	INP10, SCYB10

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	98 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
Subcellular location	Secreted.
Induction	By IFNG/IFN-gamma. A diverse population of cell types rapidly increases transcription of mRNA encoding this protein. This suggests that gamma-induced protein may be a key mediator of the IFNG/IFN-gamma response.
Post-translational modification	CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.
Sequence similarities	Belongs to the intercrine alpha (chemokine CxC) family.
Mass spectrometry	Molecular mass is 8641.8 Da from positions 22 - 98. Determined by ESI. Ref.5

Ontologies

Keywords
Biological process	Chemotaxis Inflammatory response
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine
PTM	Citrullination Disulfide bond
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	blood circulation Traceable author statement PubMed 8798675. Source: ProtInc cell surface receptor signaling pathway Traceable author statement PubMed 10903763. Source: ProtInc cell-cell signaling Traceable author statement Ref.1. Source: ProtInc cellular response to heat Inferred from electronic annotation. Source: Compara chemotaxis Traceable author statement PubMed 10903763. Source: ProtInc defense response to virus Inferred from electronic annotation. Source: Compara immune response Inferred from electronic annotation. Source: Compara inflammatory response Traceable author statement PubMed 10903763. Source: ProtInc muscle organ development Traceable author statement PubMed 8798675. Source: ProtInc negative regulation of angiogenesis Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Traceable author statement PubMed 8798675. Source: ProtInc positive regulation of leukocyte chemotaxis Inferred from electronic annotation. Source: Compara protein secretion Inferred from electronic annotation. Source: Compara response to auditory stimulus Inferred from electronic annotation. Source: Compara response to cold Inferred from electronic annotation. Source: Compara response to gamma radiation Inferred from electronic annotation. Source: Compara response to lipopolysaccharide Inferred from electronic annotation. Source: Compara response to vitamin D Inferred from electronic annotation. Source: Compara
Cellular_component	external side of plasma membrane Inferred from electronic annotation. Source: Compara extracellular region Inferred from direct assay PubMed 20041150. Source: BHF-UCL extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cAMP-dependent protein kinase regulator activity Traceable author statement PubMed 10903763. Source: ProtInc chemokine activity Traceable author statement PubMed 10903763. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

21

Ref.3 Ref.4 Ref.5

Chain

22 – 98

77

C-X-C motif chemokine 10

PRO_0000005101

Chain

22 – 94

73

CXCL10(1-73)

PRO_0000005102

Amino acid modifications

Modified residue

26

1

Citrulline; by PAD2

Disulfide bond

30 ↔ 57

Disulfide bond

32 ↔ 74

Experimental info

Sequence conflict

93

1

R → M in CAA26370. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

98

Helix Strand Turn

Details...

Turn

42 – 44

3

Beta strand

45 – 51

7

Beta strand

57 – 59

3

Beta strand

61 – 66

6

Turn

67 – 69

3

Beta strand

72 – 75

4

Beta strand

77 – 79

3

Helix

80 – 88

9

Sequences

	Sequence		Length	Mass (Da)
	P02778 [UniParc]. Last modified June 20, 2002. Version 2. Checksum: 44AE51967C58DDFF	FASTA	98	10,881
	10 20 30 40 50 60 MNQTAILICC LIFLTLSGIQ GVPLSRTVRC TCISISNQPV NPRSLEKLEI IPASQFCPRV 70 80 90 EIIATMKKKG EKRCLNPESK AIKNLLKAVS KERSKRSP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gamma-interferon transcriptionally regulates an early-response gene containing homology to platelet proteins." Luster A.D., Unkeless J.C., Ravetch J.V. Nature 315:672-676(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[3]	"Identification of a novel granulocyte chemotactic protein (GCP-2) from human tumor cells. In vitro and in vivo comparison with natural forms of GRO, IP-10, and IL-8." Proost P., de Wolf-Peeters C., Conings R., Opdenakker G., Billiau A., van Damme J. J. Immunol. 150:1000-1010(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-51.
[4]	"Human IP-9: a keratinocyte derived high affinity CXC-chemokine ligand for the IP-10/Mig receptor (CXCR3)." Tensen C.P., Flier J., van der Raaij-Helmer E.M.H., Sampat-Sardjoepersad S., van der Schors R.C., Leurs R., Scheper R.J., Boorsma D.M., Willemze R. J. Invest. Dermatol. 112:716-722(1999) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-29. Tissue: Keratinocyte.
[5]	"Processing of natural and recombinant CXCR3-targeting chemokines and implications for biological activity." Hensbergen P.J., van der Raaij-Helmer E.M.H., Dijkman R., van der Schors R.C., Werner-Felmayer G., Boorsma D.M., Scheper R.J., Willemze R., Tensen C.P. Eur. J. Biochem. 268:4992-4999(2001) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-27; 60-67 AND 79-98, MASS SPECTROMETRY, IDENTIFICATION OF CXCL10(1-73). Tissue: Foreskin keratinocyte.
[6]	"Citrullination of CXCL10 and CXCL11 by peptidylarginine deiminase: a naturally occurring posttranslational modification of chemokines and new dimension of immunoregulation." Loos T., Mortier A., Gouwy M., Ronsse I., Put W., Lenaerts J.P., Van Damme J., Proost P. Blood 112:2648-2656(2008) [PubMed] [Europe PMC] [Abstract] Cited for: CITRULLINATION AT ARG-26.
[7]	"The CXCR3 binding chemokine IP-10/CXCL10: structure and receptor interactions." Booth V., Keizer D.W., Kamphuis M.B., Clark-Lewis I., Sykes B.D. Biochemistry 41:10418-10425(2002) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 22-98.
+	Additional computationally mapped references.

Web resources

Wikipedia

CXCL10 entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X02530 mRNA. Translation: CAA26370.1.
BC010954 mRNA. Translation: AAH10954.1.

IPI

IPI00022448.

PIR

TGHUGI. A03243.

RefSeq

NP_001556.2. NM_001565.3.

UniGene

Hs.632586.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LV9	NMR	-	A	22-98	[»]
1O7Y	X-ray	3.00	A/B/C/D	22-98	[»]
1O7Z	X-ray	1.92	A/B	22-98	[»]
1O80	X-ray	2.00	A/B	22-98	[»]

ProteinModelPortal

P02778.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5893N.

MINT

MINT-91448.

STRING

9606.ENSP00000305651.

PTM databases

PhosphoSite

P02778.

Polymorphism databases

DMDM

21542456.

Proteomic databases

PaxDb

P02778.

PRIDE

P02778.

Protocols and materials databases

DNASU

3627.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000306602; ENSP00000305651; ENSG00000169245.

GeneID

3627.

KEGG

hsa:3627.

UCSC

uc003hjl.4. human.

Organism-specific databases

CTD

3627.

GeneCards

GC04M076942.

HGNC

HGNC:10637. CXCL10.

MIM

147310. gene.

neXtProt

NX_P02778.

PharmGKB

PA35568.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG47968.

HOGENOM

HOG000220915.

HOVERGEN

HBG107789.

InParanoid

P02778.

KO

K12671.

OMA

SCPRVEI.

OrthoDB

EOG4G7C0Z.

PhylomeDB

P02778.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

Bgee

P02778.

CleanEx

HS_CXCL10.

Genevestigator

P02778.

GermOnline

ENSG00000169245. Homo sapiens.

Family and domain databases

InterPro

IPR001089. Chemokine_CXC.
IPR018048. Chemokine_CXC_CS.
IPR027220. Chemokine_CXCL10.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]

PANTHER

PTHR10179. PTHR10179. 1 hit.
PTHR10179:SF5. PTHR10179:SF5. 1 hit.

Pfam

PF00048. IL8. 1 hit.
[Graphical view]

PRINTS

PR00437. SMALLCYTKCXC.

SMART

SM00199. SCY. 1 hit.
[Graphical view]

SUPFAM

SSF54117. Chemokine_IL8. 1 hit.

PROSITE

PS00471. SMALL_CYTOKINES_CXC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P02778.

GenomeRNAi

3627.

NextBio

14193.

SOURCE

Search...

Entry information

Entry name

CXL10_HUMAN

Accession

Primary (citable) accession number: P02778
Secondary accession number(s): Q96QJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	June 20, 2002
Last modified:	May 1, 2013
This is version 149 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.