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Protein

Platelet factor 4

Gene

PF4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.1 Publication

GO - Molecular functioni

  • chemokine activity Source: UniProtKB
  • CXCR3 chemokine receptor binding Source: UniProtKB
  • heparin binding Source: UniProtKB

GO - Biological processi

  • chemokine-mediated signaling pathway Source: GO_Central
  • cytokine-mediated signaling pathway Source: UniProtKB
  • G-protein coupled receptor signaling pathway Source: GO_Central
  • immune response Source: GO_Central
  • inflammatory response Source: GO_Central
  • leukocyte chemotaxis Source: UniProtKB
  • negative regulation of angiogenesis Source: UniProtKB
  • negative regulation of cytolysis Source: BHF-UCL
  • negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: BHF-UCL
  • negative regulation of megakaryocyte differentiation Source: UniProtKB
  • negative regulation of MHC class II biosynthetic process Source: BHF-UCL
  • platelet activation Source: UniProtKB
  • platelet degranulation Source: Reactome
  • positive regulation of cAMP-mediated signaling Source: UniProtKB
  • positive regulation of cAMP metabolic process Source: UniProtKB
  • positive regulation of gene expression Source: BHF-UCL
  • positive regulation of leukocyte chemotaxis Source: GO_Central
  • positive regulation of macrophage derived foam cell differentiation Source: BHF-UCL
  • positive regulation of macrophage differentiation Source: BHF-UCL
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • positive regulation of tumor necrosis factor production Source: BHF-UCL
  • regulation of cell proliferation Source: UniProtKB
  • response to lipopolysaccharide Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Chemotaxis

Keywords - Ligandi

Heparin-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000163737-MONOMER.
ReactomeiR-HSA-114608. Platelet degranulation.
R-HSA-140875. Common Pathway of Fibrin Clot Formation.
R-HSA-202733. Cell surface interactions at the vascular wall.
R-HSA-380108. Chemokine receptors bind chemokines.
R-HSA-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet factor 4
Short name:
PF-4
Alternative name(s):
C-X-C motif chemokine 4
Iroplact
Oncostatin-A
Cleaved into the following chain:
Gene namesi
Name:PF4
Synonyms:CXCL4, SCYB4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:8861. PF4.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB
  • extracellular space Source: GO_Central
  • platelet alpha granule lumen Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi5196.
OpenTargetsiENSG00000163737.
PharmGKBiPA33203.

Chemistry databases

ChEMBLiCHEMBL3286075.
DrugBankiDB00055. Drotrecogin alfa.

Polymorphism and mutation databases

BioMutaiPF4.
DMDMi130304.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 315 PublicationsAdd BLAST31
ChainiPRO_000000506832 – 101Platelet factor 4Add BLAST70
ChainiPRO_000035121748 – 101Platelet factor 4, short formAdd BLAST54

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi41 ↔ 671 Publication
Disulfide bondi43 ↔ 831 Publication
Modified residuei57PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiP02776.
PeptideAtlasiP02776.
PRIDEiP02776.
TopDownProteomicsiP02776.

2D gel databases

OGPiP02776.

PTM databases

PhosphoSitePlusiP02776.

Miscellaneous databases

PMAP-CutDBP02776.

Expressioni

Gene expression databases

BgeeiENSG00000163737.
CleanExiHS_PF4.
GenevisibleiP02776. HS.

Organism-specific databases

HPAiCAB026008.
HPA052485.

Interactioni

Subunit structurei

Homotetramer.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
APOHP027492EBI-2565740,EBI-2114682

GO - Molecular functioni

  • chemokine activity Source: UniProtKB
  • CXCR3 chemokine receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi111219. 7 interactors.
DIPiDIP-56941N.
IntActiP02776. 4 interactors.
STRINGi9606.ENSP00000296029.

Chemistry databases

BindingDBiP02776.

Structurei

Secondary structure

1101
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi42 – 44Combined sources3
Helixi52 – 54Combined sources3
Beta strandi55 – 61Combined sources7
Beta strandi64 – 66Combined sources3
Beta strandi67 – 69Combined sources3
Beta strandi71 – 76Combined sources6
Beta strandi77 – 79Combined sources3
Beta strandi81 – 83Combined sources3
Beta strandi86 – 88Combined sources3
Helixi90 – 99Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DN3NMR-A59-73[»]
1F9QX-ray2.00A/B/C/D32-101[»]
1F9RX-ray2.00A/B/C/D32-101[»]
1F9SX-ray2.38A/B/C/D32-101[»]
1PFMNMR-A/B/C/D39-101[»]
1PFNNMR-A/B/C/D39-101[»]
1RHPX-ray2.40A/B/C/D32-101[»]
4R9WX-ray2.50A/B32-101[»]
4R9YX-ray4.11A/B/C/D32-101[»]
4RAUX-ray3.74C/F/I/L/O/R/U/X32-101[»]
ProteinModelPortaliP02776.
SMRiP02776.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02776.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni92 – 98Heparin-binding7

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J314. Eukaryota.
ENOG4111556. LUCA.
GeneTreeiENSGT00530000062901.
HOGENOMiHOG000220915.
HOVERGENiHBG107789.
InParanoidiP02776.
KOiK05407.
OMAiCAVPQLI.
OrthoDBiEOG091G14JD.
PhylomeDBiP02776.
TreeFamiTF333433.

Family and domain databases

CDDicd00273. Chemokine_CXC. 1 hit.
InterProiIPR001089. Chemokine_CXC.
IPR018048. Chemokine_CXC_CS.
IPR001811. Chemokine_IL8-like_dom.
IPR033899. CXC_Chemokine_domain.
IPR027222. PF4.
[Graphical view]
PANTHERiPTHR10179. PTHR10179. 1 hit.
PTHR10179:SF38. PTHR10179:SF38. 1 hit.
PfamiPF00048. IL8. 1 hit.
[Graphical view]
PRINTSiPR00437. SMALLCYTKCXC.
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00471. SMALL_CYTOKINES_CXC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02776-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSAAGFCAS RPGLLFLGLL LLPLVVAFAS AEAEEDGDLQ CLCVKTTSQV
60 70 80 90 100
RPRHITSLEV IKAGPHCPTA QLIATLKNGR KICLDLQAPL YKKIIKKLLE

S
Length:101
Mass (Da):10,845
Last modified:July 1, 1989 - v2
Checksum:iE96C2EFE9B944D85
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78N → D AA sequence (PubMed:267922).Curated1
Sequence conflicti78N → D AA sequence (PubMed:601757).Curated1

Mass spectrometryi

Molecular mass is 7765 Da from positions 32 - 101. Determined by ESI. 1 Publication
Molecular mass is 6033 Da from positions 48 - 101. Determined by ESI. Short form.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25897 mRNA. Translation: AAA60066.1.
AF349466 Genomic DNA. Translation: AAK29643.1.
CR407677 mRNA. Translation: CAG28605.1.
AC097709 Genomic DNA. Translation: AAY41003.1.
BC093965 mRNA. Translation: AAH93965.1.
BC112093 mRNA. Translation: AAI12094.1.
CCDSiCCDS3562.1.
PIRiA60161. PFHU4.
RefSeqiNP_002610.1. NM_002619.3.
UniGeneiHs.81564.

Genome annotation databases

EnsembliENST00000296029; ENSP00000296029; ENSG00000163737.
GeneIDi5196.
KEGGihsa:5196.
UCSCiuc003hhi.4. human.

Cross-referencesi

Web resourcesi

Wikipedia

CXCL4 entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25897 mRNA. Translation: AAA60066.1.
AF349466 Genomic DNA. Translation: AAK29643.1.
CR407677 mRNA. Translation: CAG28605.1.
AC097709 Genomic DNA. Translation: AAY41003.1.
BC093965 mRNA. Translation: AAH93965.1.
BC112093 mRNA. Translation: AAI12094.1.
CCDSiCCDS3562.1.
PIRiA60161. PFHU4.
RefSeqiNP_002610.1. NM_002619.3.
UniGeneiHs.81564.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DN3NMR-A59-73[»]
1F9QX-ray2.00A/B/C/D32-101[»]
1F9RX-ray2.00A/B/C/D32-101[»]
1F9SX-ray2.38A/B/C/D32-101[»]
1PFMNMR-A/B/C/D39-101[»]
1PFNNMR-A/B/C/D39-101[»]
1RHPX-ray2.40A/B/C/D32-101[»]
4R9WX-ray2.50A/B32-101[»]
4R9YX-ray4.11A/B/C/D32-101[»]
4RAUX-ray3.74C/F/I/L/O/R/U/X32-101[»]
ProteinModelPortaliP02776.
SMRiP02776.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111219. 7 interactors.
DIPiDIP-56941N.
IntActiP02776. 4 interactors.
STRINGi9606.ENSP00000296029.

Chemistry databases

BindingDBiP02776.
ChEMBLiCHEMBL3286075.
DrugBankiDB00055. Drotrecogin alfa.

PTM databases

PhosphoSitePlusiP02776.

Polymorphism and mutation databases

BioMutaiPF4.
DMDMi130304.

2D gel databases

OGPiP02776.

Proteomic databases

PaxDbiP02776.
PeptideAtlasiP02776.
PRIDEiP02776.
TopDownProteomicsiP02776.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000296029; ENSP00000296029; ENSG00000163737.
GeneIDi5196.
KEGGihsa:5196.
UCSCiuc003hhi.4. human.

Organism-specific databases

CTDi5196.
DisGeNETi5196.
GeneCardsiPF4.
HGNCiHGNC:8861. PF4.
HPAiCAB026008.
HPA052485.
MIMi173460. gene.
neXtProtiNX_P02776.
OpenTargetsiENSG00000163737.
PharmGKBiPA33203.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410J314. Eukaryota.
ENOG4111556. LUCA.
GeneTreeiENSGT00530000062901.
HOGENOMiHOG000220915.
HOVERGENiHBG107789.
InParanoidiP02776.
KOiK05407.
OMAiCAVPQLI.
OrthoDBiEOG091G14JD.
PhylomeDBiP02776.
TreeFamiTF333433.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000163737-MONOMER.
ReactomeiR-HSA-114608. Platelet degranulation.
R-HSA-140875. Common Pathway of Fibrin Clot Formation.
R-HSA-202733. Cell surface interactions at the vascular wall.
R-HSA-380108. Chemokine receptors bind chemokines.
R-HSA-418594. G alpha (i) signalling events.

Miscellaneous databases

EvolutionaryTraceiP02776.
GeneWikiiPlatelet_factor_4.
GenomeRNAii5196.
PMAP-CutDBP02776.
PROiP02776.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000163737.
CleanExiHS_PF4.
GenevisibleiP02776. HS.

Family and domain databases

CDDicd00273. Chemokine_CXC. 1 hit.
InterProiIPR001089. Chemokine_CXC.
IPR018048. Chemokine_CXC_CS.
IPR001811. Chemokine_IL8-like_dom.
IPR033899. CXC_Chemokine_domain.
IPR027222. PF4.
[Graphical view]
PANTHERiPTHR10179. PTHR10179. 1 hit.
PTHR10179:SF38. PTHR10179:SF38. 1 hit.
PfamiPF00048. IL8. 1 hit.
[Graphical view]
PRINTSiPR00437. SMALLCYTKCXC.
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00471. SMALL_CYTOKINES_CXC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLF4_HUMAN
AccessioniPrimary (citable) accession number: P02776
Secondary accession number(s): Q53X61, Q9UC64, Q9UC65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1989
Last modified: November 30, 2016
This is version 169 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.