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P02773

- FETA_RAT

UniProt

P02773 - FETA_RAT

Protein

Alpha-fetoprotein

Gene

Afp

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Binds estrogens, fatty acids and metals.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi28 – 281CopperSequence Analysis

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. liver development Source: RGD
    2. organ regeneration Source: RGD
    3. pancreas development Source: RGD
    4. response to organic substance Source: RGD
    5. transport Source: InterPro

    Keywords - Ligandi

    Copper, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-fetoprotein
    Alternative name(s):
    Alpha-1-fetoprotein
    Alpha-fetoglobulin
    Gene namesi
    Name:Afp
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2065. Afp.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: RGD
    2. extracellular space Source: RGD

    Keywords - Cellular componenti

    Cytoplasm, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424CuratedAdd
    BLAST
    Chaini25 – 611587Alpha-fetoproteinPRO_0000001100Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi101 ↔ 116PROSITE-ProRule annotation
    Disulfide bondi115 ↔ 126PROSITE-ProRule annotation
    Glycosylationi117 – 1171N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi150 ↔ 195PROSITE-ProRule annotation
    Disulfide bondi194 ↔ 203PROSITE-ProRule annotation
    Disulfide bondi226 ↔ 272PROSITE-ProRule annotation
    Glycosylationi253 – 2531N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi271 ↔ 279PROSITE-ProRule annotation
    Disulfide bondi291 ↔ 305PROSITE-ProRule annotation
    Disulfide bondi304 ↔ 315PROSITE-ProRule annotation
    Disulfide bondi386 ↔ 395PROSITE-ProRule annotation
    Disulfide bondi418 ↔ 464PROSITE-ProRule annotation
    Disulfide bondi463 ↔ 474PROSITE-ProRule annotation
    Disulfide bondi487 ↔ 503PROSITE-ProRule annotation
    Disulfide bondi502 ↔ 513PROSITE-ProRule annotation
    Disulfide bondi540 ↔ 585PROSITE-ProRule annotation
    Disulfide bondi584 ↔ 593PROSITE-ProRule annotation

    Post-translational modificationi

    Sulfated.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Sulfation

    Proteomic databases

    PaxDbiP02773.
    PRIDEiP02773.

    Expressioni

    Tissue specificityi

    Plasma.1 Publication

    Gene expression databases

    GenevestigatoriP02773.

    Structurei

    3D structure databases

    ProteinModelPortaliP02773.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 212188Albumin 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini213 – 404192Albumin 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini405 – 603199Albumin 3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ALB/AFP/VDB family.PROSITE-ProRule annotation
    Contains 3 albumin domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG45992.
    HOGENOMiHOG000293137.
    HOVERGENiHBG004207.
    InParanoidiP02773.
    PhylomeDBiP02773.

    Family and domain databases

    InterProiIPR000264. ALB/AFP/VDB.
    IPR001703. Alpha-fetoprotein.
    IPR020858. Serum_albumin-like.
    IPR021177. Serum_albumin/AFP.
    IPR020857. Serum_albumin_CS.
    IPR014760. Serum_albumin_N.
    [Graphical view]
    PfamiPF00273. Serum_albumin. 3 hits.
    [Graphical view]
    PIRSFiPIRSF002520. Serum_albumin_subgroup. 1 hit.
    PRINTSiPR00803. AFETOPROTEIN.
    PR00802. SERUMALBUMIN.
    SMARTiSM00103. ALBUMIN. 3 hits.
    [Graphical view]
    SUPFAMiSSF48552. SSF48552. 3 hits.
    PROSITEiPS00212. ALBUMIN_1. 2 hits.
    PS51438. ALBUMIN_2. 3 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P02773-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKQPATMKWS ASISFLLLLN FAEPRVLHTN EFGIESTLDS SQCPTEKNMF    50
    NVATIVVAQF VQDATKAEVN KMSSDALAAM KENTGDGCLE NQLSVFLDEI 100
    CHETELSNKY GFSGCCNQSG VERHQCLLAR KKTAPDSVPP FHFPETAESC 150
    PAYEENRAMS INTFIYDVSK RNPFLYAPTI LYLAAQYDKA VPACCKADNM 200
    EECFQTKRAS MAKELREGSM LNEHVCAVIR KFGSRNLQAV LIIKLSQKFP 250
    KANITEIRKL ALDVAHIHEQ CCHGNAMECL QDGESVMTHM CSQQEILSSK 300
    TAECCKLPTI ELGYCIIHAE NGDKPEGLTL NPSEFLGDRN FAQFSSEEKL 350
    LFMASFLHEY SRNHPNLPVS VILKTAKSYQ EILEKCSQSE TPSKCQDNME 400
    EELQKHIQES QALAKQSCNL YQKLGPYYLQ NLFLIGYTRK APQLTSAELI 450
    DLTGKMVSIA STCCQLSEEK RSACGEGLAD IYIGHLCLRH EANPVNSGIN 500
    HCCSSSYSNR RLCITSFLRD ETYVPPPFSE DKFIFHKDLC QAQGRALQTM 550
    KQELLINLVK QKPEMTEEQH AAVTADFSGL LEKCCKDQDQ EACFAKEGPK 600
    LISKTREALG V 611
    Length:611
    Mass (Da):68,386
    Last modified:July 21, 1986 - v1
    Checksum:i7DC54624B7245C41
    GO
    Isoform 2 (identifier: P02773-2) [UniParc]FASTAAdd to Basket

    Also known as: ARFP9

    The sequence of this isoform differs from the canonical sequence as follows:
         1-286: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:325
    Mass (Da):36,526
    Checksum:i75AA117AB3EB1DFC
    GO

    Sequence cautioni

    The sequence AAA40695.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti526 – 5261P → L in CAA24546. (PubMed:6157690)Curated
    Sequence conflicti530 – 5389EDKFIFHKD → ATNSSSTRN in CAA24546. (PubMed:6157690)Curated
    Sequence conflicti547 – 5471L → P in CAA24546. (PubMed:6157690)Curated
    Sequence conflicti556 – 5572IN → VG in AAA40694. (PubMed:6157681)Curated
    Sequence conflicti598 – 6047GPKLISK → VQVDFQ in CAA24546. (PubMed:6157690)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 286286Missing in isoform 2. CuratedVSP_011552Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02361 mRNA. Translation: CAA26214.1.
    V01254 mRNA. Translation: CAA24567.1.
    X05093 Genomic DNA. Translation: CAA28744.1.
    J02816 Genomic DNA. Translation: AAA40695.1. Different initiation.
    V01236 mRNA. Translation: CAA24546.1.
    J00694 mRNA. Translation: AAA40694.1.
    PIRiA93561. FPRT.
    UniGeneiRn.9174.

    Genome annotation databases

    UCSCiRGD:2065. rat. [P02773-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02361 mRNA. Translation: CAA26214.1 .
    V01254 mRNA. Translation: CAA24567.1 .
    X05093 Genomic DNA. Translation: CAA28744.1 .
    J02816 Genomic DNA. Translation: AAA40695.1 . Different initiation.
    V01236 mRNA. Translation: CAA24546.1 .
    J00694 mRNA. Translation: AAA40694.1 .
    PIRi A93561. FPRT.
    UniGenei Rn.9174.

    3D structure databases

    ProteinModelPortali P02773.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL3141.

    Proteomic databases

    PaxDbi P02773.
    PRIDEi P02773.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:2065. rat. [P02773-1 ]

    Organism-specific databases

    RGDi 2065. Afp.

    Phylogenomic databases

    eggNOGi NOG45992.
    HOGENOMi HOG000293137.
    HOVERGENi HBG004207.
    InParanoidi P02773.
    PhylomeDBi P02773.

    Miscellaneous databases

    NextBioi 13948453.
    PROi P02773.

    Gene expression databases

    Genevestigatori P02773.

    Family and domain databases

    InterProi IPR000264. ALB/AFP/VDB.
    IPR001703. Alpha-fetoprotein.
    IPR020858. Serum_albumin-like.
    IPR021177. Serum_albumin/AFP.
    IPR020857. Serum_albumin_CS.
    IPR014760. Serum_albumin_N.
    [Graphical view ]
    Pfami PF00273. Serum_albumin. 3 hits.
    [Graphical view ]
    PIRSFi PIRSF002520. Serum_albumin_subgroup. 1 hit.
    PRINTSi PR00803. AFETOPROTEIN.
    PR00802. SERUMALBUMIN.
    SMARTi SM00103. ALBUMIN. 3 hits.
    [Graphical view ]
    SUPFAMi SSF48552. SSF48552. 3 hits.
    PROSITEi PS00212. ALBUMIN_1. 2 hits.
    PS51438. ALBUMIN_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 2), TISSUE SPECIFICITY.
      Tissue: Liver.
    2. "Rat alpha 1-fetoprotein messenger RNA: 5'-end sequence and glucocorticoid-suppressed liver transcription in an improved nuclear run-off assay."
      Turcotte B., Guertin M., Chevrette M., Belanger L.
      Nucleic Acids Res. 13:2387-2398(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-106 (ISOFORM 1).
      Strain: Sprague-Dawley.
    3. "Sequence homology between RNAs encoding rat alpha-fetoprotein and rat serum albumin."
      Jagodzinski L.L., Sargent T.D., Yang M., Glackin C., Bonner J.
      Proc. Natl. Acad. Sci. U.S.A. 78:3521-3525(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 91-611 (ISOFORM 1).
    4. "The transcription control region of the rat alpha-fetoprotein gene. DNA sequence and homology studies."
      Buzard G.S., Locker J.
      DNA Seq. 1:33-48(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 1-51 (ISOFORM 1).
    5. "The rat alpha 1-fetoprotein gene: characterization of the 5'-flanking region and tandem organization with the albumin gene."
      Chevrette M., Guertin M., Turcotte B., Belanger L.
      Nucleic Acids Res. 15:1338-1338(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-34 (ISOFORM 1).
    6. "The rat alpha-fetoprotein and albumin genes. Transcriptional control and comparison of the sequence organization and promoter region."
      Nahon J.-L., Danan J.L., Poiret M., Tratner I., Jose-Estanyol M., Sala-Trepat J.M.
      J. Biol. Chem. 262:12479-12487(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-13 (ISOFORM 1).
    7. "Alpha-fetoprotein gene expression. Partial DNA sequence and COOH-terminal homology to albumin."
      Innis M.A., Miller D.L.
      J. Biol. Chem. 255:8994-8996(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 476-611.
    8. "Amino acid sequence homology between rat alpha-fetoprotein and albumin at the COOH-terminal regions."
      Liao W.S.L., Hamilton R.W., Taylor J.M.
      J. Biol. Chem. 255:8046-8049(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 556-611.

    Entry informationi

    Entry nameiFETA_RAT
    AccessioniPrimary (citable) accession number: P02773
    Secondary accession number(s): Q63032, Q63205
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3