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Protein

Alpha-fetoprotein

Gene

Afp

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Binds estrogens, fatty acids and metals.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi28CopperSequence analysis1

GO - Molecular functioni

GO - Biological processi

  • animal organ regeneration Source: RGD
  • liver development Source: RGD
  • pancreas development Source: RGD
  • response to organic substance Source: RGD
  • transport Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-fetoprotein
Alternative name(s):
Alpha-1-fetoprotein
Alpha-fetoglobulin
Gene namesi
Name:Afp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2065. Afp.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • extracellular space Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3141.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24CuratedAdd BLAST24
ChainiPRO_000000110025 – 611Alpha-fetoproteinAdd BLAST587

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi101 ↔ 116PROSITE-ProRule annotation
Modified residuei113PhosphoserineBy similarity1
Disulfide bondi115 ↔ 126PROSITE-ProRule annotation
Glycosylationi117N-linked (GlcNAc...)Sequence analysis1
Modified residuei119PhosphoserineBy similarity1
Disulfide bondi150 ↔ 195PROSITE-ProRule annotation
Disulfide bondi194 ↔ 203PROSITE-ProRule annotation
Disulfide bondi226 ↔ 272PROSITE-ProRule annotation
Glycosylationi253N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi271 ↔ 279PROSITE-ProRule annotation
Disulfide bondi291 ↔ 305PROSITE-ProRule annotation
Disulfide bondi304 ↔ 315PROSITE-ProRule annotation
Modified residuei346PhosphoserineBy similarity1
Disulfide bondi386 ↔ 395PROSITE-ProRule annotation
Disulfide bondi418 ↔ 464PROSITE-ProRule annotation
Modified residuei446PhosphoserineBy similarity1
Disulfide bondi463 ↔ 474PROSITE-ProRule annotation
Disulfide bondi487 ↔ 503PROSITE-ProRule annotation
Disulfide bondi502 ↔ 513PROSITE-ProRule annotation
Disulfide bondi540 ↔ 585PROSITE-ProRule annotation
Disulfide bondi584 ↔ 593PROSITE-ProRule annotation

Post-translational modificationi

Sulfated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Sulfation

Proteomic databases

PaxDbiP02773.
PRIDEiP02773.

Expressioni

Tissue specificityi

Plasma.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003879.

Structurei

3D structure databases

ProteinModelPortaliP02773.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 212Albumin 1PROSITE-ProRule annotationAdd BLAST188
Domaini213 – 404Albumin 2PROSITE-ProRule annotationAdd BLAST192
Domaini405 – 603Albumin 3PROSITE-ProRule annotationAdd BLAST199

Sequence similaritiesi

Belongs to the ALB/AFP/VDB family.PROSITE-ProRule annotation
Contains 3 albumin domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IIRZ. Eukaryota.
ENOG410Z40H. LUCA.
HOGENOMiHOG000293137.
HOVERGENiHBG004207.
InParanoidiP02773.
PhylomeDBiP02773.

Family and domain databases

CDDicd00015. ALBUMIN. 3 hits.
InterProiIPR000264. ALB/AFP/VDB.
IPR020858. Serum_albumin-like.
IPR021177. Serum_albumin/AFP/Afamin.
IPR020857. Serum_albumin_CS.
IPR014760. Serum_albumin_N.
[Graphical view]
PfamiPF00273. Serum_albumin. 3 hits.
[Graphical view]
PIRSFiPIRSF002520. Serum_albumin_subgroup. 1 hit.
PRINTSiPR00803. AFETOPROTEIN.
PR00802. SERUMALBUMIN.
SMARTiSM00103. ALBUMIN. 3 hits.
[Graphical view]
SUPFAMiSSF48552. SSF48552. 3 hits.
PROSITEiPS00212. ALBUMIN_1. 2 hits.
PS51438. ALBUMIN_2. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P02773-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKQPATMKWS ASISFLLLLN FAEPRVLHTN EFGIESTLDS SQCPTEKNMF
60 70 80 90 100
NVATIVVAQF VQDATKAEVN KMSSDALAAM KENTGDGCLE NQLSVFLDEI
110 120 130 140 150
CHETELSNKY GFSGCCNQSG VERHQCLLAR KKTAPDSVPP FHFPETAESC
160 170 180 190 200
PAYEENRAMS INTFIYDVSK RNPFLYAPTI LYLAAQYDKA VPACCKADNM
210 220 230 240 250
EECFQTKRAS MAKELREGSM LNEHVCAVIR KFGSRNLQAV LIIKLSQKFP
260 270 280 290 300
KANITEIRKL ALDVAHIHEQ CCHGNAMECL QDGESVMTHM CSQQEILSSK
310 320 330 340 350
TAECCKLPTI ELGYCIIHAE NGDKPEGLTL NPSEFLGDRN FAQFSSEEKL
360 370 380 390 400
LFMASFLHEY SRNHPNLPVS VILKTAKSYQ EILEKCSQSE TPSKCQDNME
410 420 430 440 450
EELQKHIQES QALAKQSCNL YQKLGPYYLQ NLFLIGYTRK APQLTSAELI
460 470 480 490 500
DLTGKMVSIA STCCQLSEEK RSACGEGLAD IYIGHLCLRH EANPVNSGIN
510 520 530 540 550
HCCSSSYSNR RLCITSFLRD ETYVPPPFSE DKFIFHKDLC QAQGRALQTM
560 570 580 590 600
KQELLINLVK QKPEMTEEQH AAVTADFSGL LEKCCKDQDQ EACFAKEGPK
610
LISKTREALG V
Length:611
Mass (Da):68,386
Last modified:July 21, 1986 - v1
Checksum:i7DC54624B7245C41
GO
Isoform 2 (identifier: P02773-2) [UniParc]FASTAAdd to basket
Also known as: ARFP9

The sequence of this isoform differs from the canonical sequence as follows:
     1-286: Missing.

Note: No experimental confirmation available.
Show »
Length:325
Mass (Da):36,526
Checksum:i75AA117AB3EB1DFC
GO

Sequence cautioni

The sequence AAA40695 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti526P → L in CAA24546 (PubMed:6157690).Curated1
Sequence conflicti530 – 538EDKFIFHKD → ATNSSSTRN in CAA24546 (PubMed:6157690).Curated9
Sequence conflicti547L → P in CAA24546 (PubMed:6157690).Curated1
Sequence conflicti556 – 557IN → VG in AAA40694 (PubMed:6157681).Curated2
Sequence conflicti598 – 604GPKLISK → VQVDFQ in CAA24546 (PubMed:6157690).Curated7

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0115521 – 286Missing in isoform 2. CuratedAdd BLAST286

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02361 mRNA. Translation: CAA26214.1.
V01254 mRNA. Translation: CAA24567.1.
X05093 Genomic DNA. Translation: CAA28744.1.
J02816 Genomic DNA. Translation: AAA40695.1. Different initiation.
V01236 mRNA. Translation: CAA24546.1.
J00694 mRNA. Translation: AAA40694.1.
PIRiA93561. FPRT.
UniGeneiRn.9174.

Genome annotation databases

UCSCiRGD:2065. rat. [P02773-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02361 mRNA. Translation: CAA26214.1.
V01254 mRNA. Translation: CAA24567.1.
X05093 Genomic DNA. Translation: CAA28744.1.
J02816 Genomic DNA. Translation: AAA40695.1. Different initiation.
V01236 mRNA. Translation: CAA24546.1.
J00694 mRNA. Translation: AAA40694.1.
PIRiA93561. FPRT.
UniGeneiRn.9174.

3D structure databases

ProteinModelPortaliP02773.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003879.

Chemistry databases

ChEMBLiCHEMBL3141.

Proteomic databases

PaxDbiP02773.
PRIDEiP02773.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:2065. rat. [P02773-1]

Organism-specific databases

RGDi2065. Afp.

Phylogenomic databases

eggNOGiENOG410IIRZ. Eukaryota.
ENOG410Z40H. LUCA.
HOGENOMiHOG000293137.
HOVERGENiHBG004207.
InParanoidiP02773.
PhylomeDBiP02773.

Miscellaneous databases

PROiP02773.

Family and domain databases

CDDicd00015. ALBUMIN. 3 hits.
InterProiIPR000264. ALB/AFP/VDB.
IPR020858. Serum_albumin-like.
IPR021177. Serum_albumin/AFP/Afamin.
IPR020857. Serum_albumin_CS.
IPR014760. Serum_albumin_N.
[Graphical view]
PfamiPF00273. Serum_albumin. 3 hits.
[Graphical view]
PIRSFiPIRSF002520. Serum_albumin_subgroup. 1 hit.
PRINTSiPR00803. AFETOPROTEIN.
PR00802. SERUMALBUMIN.
SMARTiSM00103. ALBUMIN. 3 hits.
[Graphical view]
SUPFAMiSSF48552. SSF48552. 3 hits.
PROSITEiPS00212. ALBUMIN_1. 2 hits.
PS51438. ALBUMIN_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFETA_RAT
AccessioniPrimary (citable) accession number: P02773
Secondary accession number(s): Q63032, Q63205
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 30, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.