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P02773

- FETA_RAT

UniProt

P02773 - FETA_RAT

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Protein

Alpha-fetoprotein

Gene

Afp

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Binds estrogens, fatty acids and metals.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi28 – 281CopperSequence Analysis

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. liver development Source: RGD
  2. organ regeneration Source: RGD
  3. pancreas development Source: RGD
  4. response to organic substance Source: RGD
  5. transport Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-fetoprotein
Alternative name(s):
Alpha-1-fetoprotein
Alpha-fetoglobulin
Gene namesi
Name:Afp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2065. Afp.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. extracellular space Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424CuratedAdd
BLAST
Chaini25 – 611587Alpha-fetoproteinPRO_0000001100Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi101 ↔ 116PROSITE-ProRule annotation
Disulfide bondi115 ↔ 126PROSITE-ProRule annotation
Glycosylationi117 – 1171N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi150 ↔ 195PROSITE-ProRule annotation
Disulfide bondi194 ↔ 203PROSITE-ProRule annotation
Disulfide bondi226 ↔ 272PROSITE-ProRule annotation
Glycosylationi253 – 2531N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi271 ↔ 279PROSITE-ProRule annotation
Disulfide bondi291 ↔ 305PROSITE-ProRule annotation
Disulfide bondi304 ↔ 315PROSITE-ProRule annotation
Disulfide bondi386 ↔ 395PROSITE-ProRule annotation
Disulfide bondi418 ↔ 464PROSITE-ProRule annotation
Disulfide bondi463 ↔ 474PROSITE-ProRule annotation
Disulfide bondi487 ↔ 503PROSITE-ProRule annotation
Disulfide bondi502 ↔ 513PROSITE-ProRule annotation
Disulfide bondi540 ↔ 585PROSITE-ProRule annotation
Disulfide bondi584 ↔ 593PROSITE-ProRule annotation

Post-translational modificationi

Sulfated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Sulfation

Proteomic databases

PaxDbiP02773.
PRIDEiP02773.

Expressioni

Tissue specificityi

Plasma.1 Publication

Gene expression databases

GenevestigatoriP02773.

Structurei

3D structure databases

ProteinModelPortaliP02773.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 212188Albumin 1PROSITE-ProRule annotationAdd
BLAST
Domaini213 – 404192Albumin 2PROSITE-ProRule annotationAdd
BLAST
Domaini405 – 603199Albumin 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ALB/AFP/VDB family.PROSITE-ProRule annotation
Contains 3 albumin domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG45992.
HOGENOMiHOG000293137.
HOVERGENiHBG004207.
InParanoidiP02773.
PhylomeDBiP02773.

Family and domain databases

InterProiIPR000264. ALB/AFP/VDB.
IPR001703. Alpha-fetoprotein.
IPR020858. Serum_albumin-like.
IPR021177. Serum_albumin/AFP.
IPR020857. Serum_albumin_CS.
IPR014760. Serum_albumin_N.
[Graphical view]
PfamiPF00273. Serum_albumin. 3 hits.
[Graphical view]
PIRSFiPIRSF002520. Serum_albumin_subgroup. 1 hit.
PRINTSiPR00803. AFETOPROTEIN.
PR00802. SERUMALBUMIN.
SMARTiSM00103. ALBUMIN. 3 hits.
[Graphical view]
SUPFAMiSSF48552. SSF48552. 3 hits.
PROSITEiPS00212. ALBUMIN_1. 2 hits.
PS51438. ALBUMIN_2. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P02773-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKQPATMKWS ASISFLLLLN FAEPRVLHTN EFGIESTLDS SQCPTEKNMF
60 70 80 90 100
NVATIVVAQF VQDATKAEVN KMSSDALAAM KENTGDGCLE NQLSVFLDEI
110 120 130 140 150
CHETELSNKY GFSGCCNQSG VERHQCLLAR KKTAPDSVPP FHFPETAESC
160 170 180 190 200
PAYEENRAMS INTFIYDVSK RNPFLYAPTI LYLAAQYDKA VPACCKADNM
210 220 230 240 250
EECFQTKRAS MAKELREGSM LNEHVCAVIR KFGSRNLQAV LIIKLSQKFP
260 270 280 290 300
KANITEIRKL ALDVAHIHEQ CCHGNAMECL QDGESVMTHM CSQQEILSSK
310 320 330 340 350
TAECCKLPTI ELGYCIIHAE NGDKPEGLTL NPSEFLGDRN FAQFSSEEKL
360 370 380 390 400
LFMASFLHEY SRNHPNLPVS VILKTAKSYQ EILEKCSQSE TPSKCQDNME
410 420 430 440 450
EELQKHIQES QALAKQSCNL YQKLGPYYLQ NLFLIGYTRK APQLTSAELI
460 470 480 490 500
DLTGKMVSIA STCCQLSEEK RSACGEGLAD IYIGHLCLRH EANPVNSGIN
510 520 530 540 550
HCCSSSYSNR RLCITSFLRD ETYVPPPFSE DKFIFHKDLC QAQGRALQTM
560 570 580 590 600
KQELLINLVK QKPEMTEEQH AAVTADFSGL LEKCCKDQDQ EACFAKEGPK
610
LISKTREALG V
Length:611
Mass (Da):68,386
Last modified:July 21, 1986 - v1
Checksum:i7DC54624B7245C41
GO
Isoform 2 (identifier: P02773-2) [UniParc]FASTAAdd to Basket

Also known as: ARFP9

The sequence of this isoform differs from the canonical sequence as follows:
     1-286: Missing.

Note: No experimental confirmation available.

Show »
Length:325
Mass (Da):36,526
Checksum:i75AA117AB3EB1DFC
GO

Sequence cautioni

The sequence AAA40695.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti526 – 5261P → L in CAA24546. (PubMed:6157690)Curated
Sequence conflicti530 – 5389EDKFIFHKD → ATNSSSTRN in CAA24546. (PubMed:6157690)Curated
Sequence conflicti547 – 5471L → P in CAA24546. (PubMed:6157690)Curated
Sequence conflicti556 – 5572IN → VG in AAA40694. (PubMed:6157681)Curated
Sequence conflicti598 – 6047GPKLISK → VQVDFQ in CAA24546. (PubMed:6157690)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 286286Missing in isoform 2. CuratedVSP_011552Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02361 mRNA. Translation: CAA26214.1.
V01254 mRNA. Translation: CAA24567.1.
X05093 Genomic DNA. Translation: CAA28744.1.
J02816 Genomic DNA. Translation: AAA40695.1. Different initiation.
V01236 mRNA. Translation: CAA24546.1.
J00694 mRNA. Translation: AAA40694.1.
PIRiA93561. FPRT.
UniGeneiRn.9174.

Genome annotation databases

UCSCiRGD:2065. rat. [P02773-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02361 mRNA. Translation: CAA26214.1 .
V01254 mRNA. Translation: CAA24567.1 .
X05093 Genomic DNA. Translation: CAA28744.1 .
J02816 Genomic DNA. Translation: AAA40695.1 . Different initiation.
V01236 mRNA. Translation: CAA24546.1 .
J00694 mRNA. Translation: AAA40694.1 .
PIRi A93561. FPRT.
UniGenei Rn.9174.

3D structure databases

ProteinModelPortali P02773.
ModBasei Search...
MobiDBi Search...

Chemistry

ChEMBLi CHEMBL3141.

Proteomic databases

PaxDbi P02773.
PRIDEi P02773.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:2065. rat. [P02773-1 ]

Organism-specific databases

RGDi 2065. Afp.

Phylogenomic databases

eggNOGi NOG45992.
HOGENOMi HOG000293137.
HOVERGENi HBG004207.
InParanoidi P02773.
PhylomeDBi P02773.

Miscellaneous databases

NextBioi 13948453.
PROi P02773.

Gene expression databases

Genevestigatori P02773.

Family and domain databases

InterProi IPR000264. ALB/AFP/VDB.
IPR001703. Alpha-fetoprotein.
IPR020858. Serum_albumin-like.
IPR021177. Serum_albumin/AFP.
IPR020857. Serum_albumin_CS.
IPR014760. Serum_albumin_N.
[Graphical view ]
Pfami PF00273. Serum_albumin. 3 hits.
[Graphical view ]
PIRSFi PIRSF002520. Serum_albumin_subgroup. 1 hit.
PRINTSi PR00803. AFETOPROTEIN.
PR00802. SERUMALBUMIN.
SMARTi SM00103. ALBUMIN. 3 hits.
[Graphical view ]
SUPFAMi SSF48552. SSF48552. 3 hits.
PROSITEi PS00212. ALBUMIN_1. 2 hits.
PS51438. ALBUMIN_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 2), TISSUE SPECIFICITY.
    Tissue: Liver.
  2. "Rat alpha 1-fetoprotein messenger RNA: 5'-end sequence and glucocorticoid-suppressed liver transcription in an improved nuclear run-off assay."
    Turcotte B., Guertin M., Chevrette M., Belanger L.
    Nucleic Acids Res. 13:2387-2398(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-106 (ISOFORM 1).
    Strain: Sprague-Dawley.
  3. "Sequence homology between RNAs encoding rat alpha-fetoprotein and rat serum albumin."
    Jagodzinski L.L., Sargent T.D., Yang M., Glackin C., Bonner J.
    Proc. Natl. Acad. Sci. U.S.A. 78:3521-3525(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 91-611 (ISOFORM 1).
  4. "The transcription control region of the rat alpha-fetoprotein gene. DNA sequence and homology studies."
    Buzard G.S., Locker J.
    DNA Seq. 1:33-48(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 1-51 (ISOFORM 1).
  5. "The rat alpha 1-fetoprotein gene: characterization of the 5'-flanking region and tandem organization with the albumin gene."
    Chevrette M., Guertin M., Turcotte B., Belanger L.
    Nucleic Acids Res. 15:1338-1338(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-34 (ISOFORM 1).
  6. "The rat alpha-fetoprotein and albumin genes. Transcriptional control and comparison of the sequence organization and promoter region."
    Nahon J.-L., Danan J.L., Poiret M., Tratner I., Jose-Estanyol M., Sala-Trepat J.M.
    J. Biol. Chem. 262:12479-12487(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-13 (ISOFORM 1).
  7. "Alpha-fetoprotein gene expression. Partial DNA sequence and COOH-terminal homology to albumin."
    Innis M.A., Miller D.L.
    J. Biol. Chem. 255:8994-8996(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 476-611.
  8. "Amino acid sequence homology between rat alpha-fetoprotein and albumin at the COOH-terminal regions."
    Liao W.S.L., Hamilton R.W., Taylor J.M.
    J. Biol. Chem. 255:8046-8049(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 556-611.

Entry informationi

Entry nameiFETA_RAT
AccessioniPrimary (citable) accession number: P02773
Secondary accession number(s): Q63032, Q63205
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 1, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3