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P02773 (FETA_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Alpha-fetoprotein
Alternative name(s):
Alpha-1-fetoprotein
Alpha-fetoglobulin
Gene names
Name:Afp
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length611 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Binds estrogens, fatty acids and metals.

Subcellular location

Isoform 1: Secreted.

Isoform 2: Cytoplasm.

Tissue specificity

Plasma. Ref.1

Post-translational modification

Sulfated By similarity.

Sequence similarities

Belongs to the ALB/AFP/VDB family.

Contains 3 albumin domains.

Sequence caution

The sequence AAA40695.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P02773-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P02773-2)

Also known as: ARFP9;

The sequence of this isoform differs from the canonical sequence as follows:
     1-286: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Probable
Chain25 – 611587Alpha-fetoprotein
PRO_0000001100

Regions

Domain25 – 212188Albumin 1
Domain213 – 404192Albumin 2
Domain405 – 603199Albumin 3

Sites

Metal binding281Copper Potential

Amino acid modifications

Glycosylation1171N-linked (GlcNAc...) Potential
Glycosylation2531N-linked (GlcNAc...) Potential
Disulfide bond101 ↔ 116 By similarity
Disulfide bond115 ↔ 126 By similarity
Disulfide bond150 ↔ 195 By similarity
Disulfide bond194 ↔ 203 By similarity
Disulfide bond226 ↔ 272 By similarity
Disulfide bond271 ↔ 279 By similarity
Disulfide bond291 ↔ 305 By similarity
Disulfide bond304 ↔ 315 By similarity
Disulfide bond386 ↔ 395 By similarity
Disulfide bond418 ↔ 464 By similarity
Disulfide bond463 ↔ 474 By similarity
Disulfide bond487 ↔ 503 By similarity
Disulfide bond502 ↔ 513 By similarity
Disulfide bond540 ↔ 585 By similarity
Disulfide bond584 ↔ 593 By similarity

Natural variations

Alternative sequence1 – 286286Missing in isoform 2.
VSP_011552

Experimental info

Sequence conflict5261P → L in CAA24546. Ref.7
Sequence conflict530 – 5389EDKFIFHKD → ATNSSSTRN in CAA24546. Ref.7
Sequence conflict5471L → P in CAA24546. Ref.7
Sequence conflict556 – 5572IN → VG in AAA40694. Ref.8
Sequence conflict598 – 6047GPKLISK → VQVDFQ in CAA24546. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 7DC54624B7245C41

FASTA61168,386
        10         20         30         40         50         60 
MKQPATMKWS ASISFLLLLN FAEPRVLHTN EFGIESTLDS SQCPTEKNMF NVATIVVAQF 

        70         80         90        100        110        120 
VQDATKAEVN KMSSDALAAM KENTGDGCLE NQLSVFLDEI CHETELSNKY GFSGCCNQSG 

       130        140        150        160        170        180 
VERHQCLLAR KKTAPDSVPP FHFPETAESC PAYEENRAMS INTFIYDVSK RNPFLYAPTI 

       190        200        210        220        230        240 
LYLAAQYDKA VPACCKADNM EECFQTKRAS MAKELREGSM LNEHVCAVIR KFGSRNLQAV 

       250        260        270        280        290        300 
LIIKLSQKFP KANITEIRKL ALDVAHIHEQ CCHGNAMECL QDGESVMTHM CSQQEILSSK 

       310        320        330        340        350        360 
TAECCKLPTI ELGYCIIHAE NGDKPEGLTL NPSEFLGDRN FAQFSSEEKL LFMASFLHEY 

       370        380        390        400        410        420 
SRNHPNLPVS VILKTAKSYQ EILEKCSQSE TPSKCQDNME EELQKHIQES QALAKQSCNL 

       430        440        450        460        470        480 
YQKLGPYYLQ NLFLIGYTRK APQLTSAELI DLTGKMVSIA STCCQLSEEK RSACGEGLAD 

       490        500        510        520        530        540 
IYIGHLCLRH EANPVNSGIN HCCSSSYSNR RLCITSFLRD ETYVPPPFSE DKFIFHKDLC 

       550        560        570        580        590        600 
QAQGRALQTM KQELLINLVK QKPEMTEEQH AAVTADFSGL LEKCCKDQDQ EACFAKEGPK 

       610 
LISKTREALG V

« Hide

Isoform 2 (ARFP9) [UniParc].

Checksum: 75AA117AB3EB1DFC
Show »

FASTA32536,526

References

[1]"Characterization of a rat variant alpha-fetoprotein."
Watanabe T., Jimenez-Molina J.L., Chou J.Y.
Biochem. Biophys. Res. Commun. 185:648-656(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 2), TISSUE SPECIFICITY.
Tissue: Liver.
[2]"Rat alpha 1-fetoprotein messenger RNA: 5'-end sequence and glucocorticoid-suppressed liver transcription in an improved nuclear run-off assay."
Turcotte B., Guertin M., Chevrette M., Belanger L.
Nucleic Acids Res. 13:2387-2398(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-106 (ISOFORM 1).
Strain: Sprague-Dawley.
[3]"Sequence homology between RNAs encoding rat alpha-fetoprotein and rat serum albumin."
Jagodzinski L.L., Sargent T.D., Yang M., Glackin C., Bonner J.
Proc. Natl. Acad. Sci. U.S.A. 78:3521-3525(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 91-611 (ISOFORM 1).
[4]"The transcription control region of the rat alpha-fetoprotein gene. DNA sequence and homology studies."
Buzard G.S., Locker J.
DNA Seq. 1:33-48(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 1-51 (ISOFORM 1).
[5]"The rat alpha 1-fetoprotein gene: characterization of the 5'-flanking region and tandem organization with the albumin gene."
Chevrette M., Guertin M., Turcotte B., Belanger L.
Nucleic Acids Res. 15:1338-1338(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-34 (ISOFORM 1).
[6]"The rat alpha-fetoprotein and albumin genes. Transcriptional control and comparison of the sequence organization and promoter region."
Nahon J.-L., Danan J.L., Poiret M., Tratner I., Jose-Estanyol M., Sala-Trepat J.M.
J. Biol. Chem. 262:12479-12487(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-13 (ISOFORM 1).
[7]"Alpha-fetoprotein gene expression. Partial DNA sequence and COOH-terminal homology to albumin."
Innis M.A., Miller D.L.
J. Biol. Chem. 255:8994-8996(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 476-611.
[8]"Amino acid sequence homology between rat alpha-fetoprotein and albumin at the COOH-terminal regions."
Liao W.S.L., Hamilton R.W., Taylor J.M.
J. Biol. Chem. 255:8046-8049(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 556-611.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X02361 mRNA. Translation: CAA26214.1.
V01254 mRNA. Translation: CAA24567.1.
X05093 Genomic DNA. Translation: CAA28744.1.
J02816 Genomic DNA. Translation: AAA40695.1. Different initiation.
V01236 mRNA. Translation: CAA24546.1.
J00694 mRNA. Translation: AAA40694.1.
IPIIPI00191740.
IPI00464445.
PIRFPRT. A93561.
UniGeneRn.9174.

3D structure databases

ProteinModelPortalP02773.
ModBaseSearch...

Proteomic databases

PaxDbP02773.
PRIDEP02773.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:2065. rat.

Organism-specific databases

RGD2065. Afp.

Phylogenomic databases

eggNOGNOG45992.
HOGENOMHOG000293137.
HOVERGENHBG004207.
InParanoidP02773.
OrthoDBEOG44F68N.

Gene expression databases

ArrayExpressP02773.
GenevestigatorP02773.
GermOnlineENSRNOG00000002889. Rattus norvegicus.

Family and domain databases

InterProIPR000264. ALB/AFP/VDB.
IPR001703. Alpha-fetoprotein.
IPR020858. Serum_albumin-like.
IPR021177. Serum_albumin/AFP.
IPR020857. Serum_albumin_CS.
IPR014760. Serum_albumin_N.
[Graphical view]
PfamPF00273. Serum_albumin. 3 hits.
[Graphical view]
PIRSFPIRSF002520. Serum_albumin_subgroup. 1 hit.
PRINTSPR00803. AFETOPROTEIN.
PR00802. SERUMALBUMIN.
SMARTSM00103. ALBUMIN. 3 hits.
[Graphical view]
SUPFAMSSF48552. Serum_albumin. 3 hits.
PROSITEPS00212. ALBUMIN_1. 2 hits.
PS51438. ALBUMIN_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

ChEMBLCHEMBL3141.
NextBio13948453.

Entry information

Entry nameFETA_RAT
AccessionPrimary (citable) accession number: P02773
Secondary accession number(s): Q63032, Q63205
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 1, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents