Reviewed, UniProtKB/Swiss-Prot P02763 (A1AG1_HUMAN)

Names and origin

Protein names

Recommended name:
    Alpha-1-acid glycoprotein 1
      Short name=AGP 1
Alternative name(s):
    Orosomucoid-1
      Short name=OMD 1

Gene names

Name:	ORM1
Synonyms:	AGP1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Appears to function in modulating the activity of the immune system during the acute-phase reaction.
Subcellular location	Secreted.
Tissue specificity	Expressed by the liver and secreted in plasma.
Induction	Synthesis is controlled by glucocorticoids, interleukin-1 and interleukin-6, It increases 5- to 50-fold upon inflammation.
Polymorphism	Three common alleles of ORM1 are known. ORM1*F1 has Gln-38/Val-174; ORM1*F2 has Gln-38/Met-174 and ORM1*S has Arg-38/Val-174. The sequence shown is that of allele ORM1*F1.
Sequence similarities	Belongs to the calycin superfamily. Lipocalin family.

Ontologies

Keywords
Biological process	Acute phase
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
PTM	Disulfide bond Glycoprotein Pyrrolidone carboxylic acid
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	acute-phase response Ref.1 Traceable author statement. Source: ProtInc regulation of immune system process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SERPINE1	P05121	1	EBI-976767,EBI-953978

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 18

18

Ref.7

Chain

19 – 201

183

Alpha-1-acid glycoprotein 1

PRO_0000017860

Amino acid modifications

Modified residue

19

1

Pyrrolidone carboxylic acid Ref.7

Glycosylation

33

1

N-linked (GlcNAc...) Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.9

Glycosylation

56

1

N-linked (GlcNAc...) Ref.10 Ref.14 Ref.15 Ref.9

Glycosylation

72

1

N-linked (GlcNAc...) Ref.10 Ref.12 Ref.14 Ref.15 Ref.9

Glycosylation

93

1

N-linked (GlcNAc...) Ref.10 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.9

Glycosylation

103

1

N-linked (GlcNAc...) Ref.10 Ref.14 Ref.15 Ref.17

CAR_000170

Disulfide bond

23 ↔ 165

Ref.9

Disulfide bond

90 ↔ 183

Ref.9

Natural variations

Natural variant

38

1

Q → R in allele ORM1*S. Ref.6 Ref.19

VAR_013840

Natural variant

167

1

R → C: dbSNP rs3182034.

VAR_056166

Natural variant

174

1

V → M in allele ORM1*F2. dbSNP rs1126801. Ref.19

VAR_013841

Experimental info

Sequence conflict

182

1

Missing AA sequence Ref.8

Sequence conflict

193

1

Missing AA sequence Ref.8

Secondary structure

1

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201

Helix Strand Turn

Details...

Helix

24 – 26

3

Helix

33 – 39

7

Beta strand

43 – 48

6

Helix

53 – 59

7

Beta strand

62 – 72

11

Turn

73 – 76

4

Beta strand

77 – 86

10

Beta strand

89 – 99

11

Beta strand

106 – 109

4

Beta strand

114 – 121

8

Beta strand

127 – 131

5

Helix

137 – 139

3

Beta strand

143 – 149

7

Turn

153 – 155

3

Helix

159 – 166

8

Helix

170 – 172

3

Helix

178 – 180

3

Helix

184 – 189

6

Sequences

	Sequence		Length	Mass (Da)
	P02763-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 63292233AD6EAD8B	FASTA	201	23,512
	10 20 30 40 50 60 MALSWVLTVL SLLPLLEAQI PLCANLVPVP ITNATLDQIT GKWFYIASAF RNEEYNKSVQ 70 80 90 100 110 120 EIQATFFYFT PNKTEDTIFL REYQTRQDQC IYNTTYLNVQ RENGTISRYV GGQEHFAHLL 130 140 150 160 170 180 ILRDTKTYML AFDVNDEKNW GLSVYADKPE TTKEQLGEFY EALDCLRIPK SDVVYTDWKK 190 200 DKCEPLEKQH EKERKQEEGE S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and expression of the genes coding for human alpha 1-acid glycoprotein." Dente L., Pizza M.G., Metspalu A., Cortese R. EMBO J. 6:2289-2296(1987) [PubMed: 2822385] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular cloning and nucleotide sequence of human alpha 1 acid glycoprotein cDNA." Board P.G., Jones I.M., Bentley A.K. Gene 44:127-131(1986) [PubMed: 3770479] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Structure of the human alpha 1-acid glycoprotein gene: sequence homology with other human acute phase protein genes." Dente L., Ciliberto G., Cortese R. Nucleic Acids Res. 13:3941-3952(1985) [PubMed: 2409529] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-38. Tissue: Liver.
[7]	"Structure of alpha 1-acid glycoprotein. The complete amino acid sequence, multiple amino acid substitutions, and homology with the immunoglobulins." Schmid K., Kaufmann H., Isemura S., Bauer F., Emura J., Motoyama T., Ishiguro M., Nanno S. Biochemistry 12:2711-2724(1973) [PubMed: 4711474] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-129.
[8]	"Isolation and partial characterization of the cyanogen bromide fragments of alpha 1-acid glycoprotein and the elucidation of the amino acid sequence of the carboxyl-terminal cyanogen bromide fragment." Ikenaka T., Ishiguro M., Emura J., Kaufmann H., Isemura S., Bauer W., Schmid K. Biochemistry 11:3817-3829(1972) [PubMed: 4561179] [Abstract] Cited for: PROTEIN SEQUENCE OF 129-201.
[9]	"The disulfide bonds of alpha1-acid glycoprotein." Schmid K., Buergi W., Collins J.H., Nanno S. Biochemistry 13:2694-2697(1974) [PubMed: 4603214] [Abstract] Cited for: DISULFIDE BONDS.
[10]	"Analysis of the five glycosylation sites of human alpha 1-acid glycoprotein." Treuheit M.J., Costello C.E., Halsall H.B. Biochem. J. 283:105-112(1992) [PubMed: 1567356] [Abstract] Cited for: GLYCOSYLATION AT ASN-33; ASN-56; ASN-72; ASN-93 AND ASN-103.
[11]	"Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry." Zhang H., Li X.-J., Martin D.B., Aebersold R. Nat. Biotechnol. 21:660-666(2003) [PubMed: 12754519] [Abstract] Cited for: GLYCOSYLATION AT ASN-33.
[12]	"A new strategy for identification of N-glycosylated proteins and unambiguous assignment of their glycosylation sites using HILIC enrichment and partial deglycosylation." Hagglund P., Bunkenborg J., Elortza F., Jensen O.N., Roepstorff P. J. Proteome Res. 3:556-566(2004) [PubMed: 15253437] [Abstract] Cited for: GLYCOSYLATION AT ASN-33; ASN-72 AND ASN-93, MASS SPECTROMETRY.
[13]	"A proteomic analysis of human bile." Kristiansen T.Z., Bunkenborg J., Gronborg M., Molina H., Thuluvath P.J., Argani P., Goggins M.G., Maitra A., Pandey A. Mol. Cell. Proteomics 3:715-728(2004) [PubMed: 15084671] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33 AND ASN-93, MASS SPECTROMETRY. Tissue: Bile.
[14]	"Screening for N-glycosylated proteins by liquid chromatography mass spectrometry." Bunkenborg J., Pilch B.J., Podtelejnikov A.V., Wisniewski J.R. Proteomics 4:454-465(2004) [PubMed: 14760718] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33; ASN-56; ASN-72; ASN-93 AND ASN-103, MASS SPECTROMETRY. Tissue: Plasma.
[15]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed: 16335952] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33; ASN-56; ASN-72; ASN-93 AND ASN-103, MASS SPECTROMETRY. Tissue: Plasma.
[16]	"Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry." Ramachandran P., Boontheung P., Xie Y., Sondej M., Wong D.T., Loo J.A. J. Proteome Res. 5:1493-1503(2006) [PubMed: 16740002] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-93, MASS SPECTROMETRY. Tissue: Saliva.
[17]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-93 AND ASN-103, MASS SPECTROMETRY. Tissue: Liver.
[18]	"Three-dimensional modeling of the protein moiety of human alpha1-acid glycoprotein, a lipocalin-family member." Rojo-Dominguez A., Hernandez-Arana A. Protein Seq. Data Anal. 5:349-355(1993) Cited for: 3D-STRUCTURE MODELING.
[19]	"Human orosomucoid polymorphism: molecular basis of the three common ORM1 alleles, ORM1*F1, ORM1*F2, and ORM1*S." Yuasa I., Umetsu K., Vogt U., Nakamura H., Nanba E., Tamaki N., Irizawa Y. Hum. Genet. 99:393-398(1997) [PubMed: 9050929] [Abstract] Cited for: VARIANTS ARG-38 AND MET-174.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X02544 mRNA. Translation: CAA26397.1.
M13692 mRNA. Translation: AAA35515.1.
X05779 , X05780, X05781, X05782, X05783, X05784 Genomic DNA. Translation: CAA29229.1. Sequence problems.
X06676 Genomic DNA. No translation available.
X06680 Genomic DNA. No translation available.
BT019790 mRNA. Translation: AAV38593.1.
AK312035 mRNA. Translation: BAG34972.1.
BC026238 mRNA. Translation: AAH26238.1.

IPI

IPI00022429.

PIR

OMHU1. A28346.

RefSeq

NP_000598.2.

UniGene

Hs.522356

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3BX6	X-ray	1.80	A	19-201	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P02763. 2 interactions.

STRING

P02763.

PTM databases

GlycoSuiteDB

P02763.

2-D gel databases

SWISS-2DPAGE

P02763.

Siena-2DPAGE

P02763.

Proteomic databases

PeptideAtlas

P02763.

PRIDE

P02763.

Genome annotation databases

Ensembl

ENST00000259396; ENSP00000259396; ENSG00000229314; Homo sapiens. [Genome view]
ENST00000438927; ENSP00000388265; ENSG00000229314; Homo sapiens. [Genome view]

GeneID

5004.

KEGG

hsa:5004.

Organism-specific databases

CTD

5004.

GeneCards

GC09P116125.

HGNC

HGNC:8498. ORM1.

HPA

CAB006265.

MIM

138600. gene.

PharmGKB

PA260.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P02763.

HOVERGEN

P02763.

Gene expression databases

ArrayExpress

P02763.

Bgee

P02763.

CleanEx

HS_ORM1.

Genevestigator

P02763.

GermOnline

ENSG00000187681. Homo sapiens.

Family and domain databases

InterPro

IPR001500. A1A_glycop.
IPR012674. Calycin.
IPR002345. Lipocalin.
IPR000566. Lipocln_cytFABP.
[Graphical view]

Gene3D

G3DSA:2.40.128.20. Calycin. 1 hit.

PANTHER

PTHR11967. A1A_glycop. 1 hit.

Pfam

PF00061. Lipocalin. 1 hit.
[Graphical view]

PIRSF

PIRSF036899. AGP. 1 hit.

PRINTS

PR00708. A1AGLPROTEIN.

PROSITE

PS00213. LIPOCALIN. False negative.
[Graphical view]

ProtoNet

Search...

Other Resources

DrugBank

DB01418. Acenocoumarol.
DB00802. Alfentanil.
DB01429. Aprindine.
DB00280. Disopyramide.
DB01359. Penbutolol.
DB00946. Phenprocoumon.
DB00908. Quinidine.
DB00706. Tamsulosin.

NextBio

19272.

SOURCE

Search...

Entry information

Entry name

A1AG1_HUMAN

Accession

Primary (citable) accession number: P02763
Secondary accession number(s): Q5U067, Q8TC16

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 1, 1988
Last modified:	October 13, 2009
This is version 118 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.