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P02761

- MUP_RAT

UniProt

P02761 - MUP_RAT

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Protein

Major urinary protein

Gene
N/A
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Major urinary proteins (Mups) bind and release pheromones. They may also protect pheromones from oxidation. In this context, they play a role in the regulation of social behaviors, such as aggression, mating, pup-suckling, territory establishment and dominance. Acts as a kairomone, detected by the prey vomeronasal organ and inducing fear reactions in mice.

GO - Molecular functioni

  1. insulin-activated receptor activity Source: UniProtKB
  2. pheromone binding Source: UniProtKB
  3. small molecule binding Source: InterPro
  4. transporter activity Source: InterPro

GO - Biological processi

  1. aerobic respiration Source: UniProtKB
  2. cellular response to lipid Source: UniProtKB
  3. energy reserve metabolic process Source: UniProtKB
  4. glucose homeostasis Source: UniProtKB
  5. heat generation Source: UniProtKB
  6. insulin receptor signaling pathway Source: GOC
  7. locomotor rhythm Source: UniProtKB
  8. mitochondrion morphogenesis Source: UniProtKB
  9. negative regulation of gluconeogenesis Source: UniProtKB
  10. negative regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
  11. negative regulation of lipid biosynthetic process Source: UniProtKB
  12. negative regulation of lipid storage Source: UniProtKB
  13. negative regulation of transcription, DNA-templated Source: UniProtKB
  14. positive regulation of gene expression Source: UniProtKB
  15. positive regulation of glucose metabolic process Source: UniProtKB
  16. positive regulation of lipid metabolic process Source: UniProtKB
  17. positive regulation of protein kinase B signaling Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Behavior, Transport

Keywords - Ligandi

Pheromone-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Major urinary protein
Short name:
MUP
Alternative name(s):
Allergen Rat n I
Alpha(2)-euglobulin
Alpha-2u-globulin
alpha-2u globulin PGCL1
Allergen: Rat n 1
Cleaved into the following chain:
15.5 kDa fatty acid-binding protein
Short name:
15.5 kDa FABP
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi708506. LOC259246.

Subcellular locationi

Chain 15.5 kDa fatty acid-binding protein : Cytoplasmcytosol
Note: It is probably taken up from the urinary lumen by endocytosis.

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. extracellular space Source: UniProtKB
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3464. Rat n 1.0101.
611. Rat n 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 19192 PublicationsAdd
BLAST
Chaini20 – 181162Major urinary proteinPRO_0000017925Add
BLAST
Chaini29 – 17915115.5 kDa fatty acid-binding proteinPRO_0000017926Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi54 – 541N-linked (GlcNAc...)Curated
Disulfide bondi83 ↔ 1761 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP02761.
PRIDEiP02761.

Expressioni

Tissue specificityi

Abundant in the urine of adult male rats but absent from that of females.

Inductioni

Synthesis of this protein in the hepatic parenchymal cells is induced in vivo by androgens, glucocorticoids, growth hormone, and insulin, and inhibited by estrogens.

Gene expression databases

GenevestigatoriP02761.

Structurei

Secondary structure

1
181
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni24 – 274
Helixi31 – 344
Beta strandi39 – 446
Helixi48 – 503
Beta strandi60 – 678
Beta strandi70 – 8112
Beta strandi83 – 9210
Beta strandi99 – 11416
Beta strandi116 – 12813
Beta strandi131 – 14414
Helixi147 – 15812
Turni159 – 1613
Helixi164 – 1663
Beta strandi167 – 1693
Helixi170 – 1723

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2A2GX-ray2.90A/B/C/D1-181[»]
2A2UX-ray2.50A/B/C/D1-181[»]
ProteinModelPortaliP02761.
SMRiP02761. Positions 20-177.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02761.

Family & Domainsi

Sequence similaritiesi

Belongs to the calycin superfamily. Lipocalin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG45000.
HOGENOMiHOG000231458.
HOVERGENiHBG000215.
InParanoidiP02761.
PhylomeDBiP02761.
TreeFamiTF338197.

Family and domain databases

Gene3Di2.40.128.20. 1 hit.
InterProiIPR012674. Calycin.
IPR011038. Calycin-like.
IPR002345. Lipocalin.
IPR022272. Lipocalin_CS.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
IPR002971. Maj_urinary.
[Graphical view]
PfamiPF00061. Lipocalin. 1 hit.
[Graphical view]
PRINTSiPR00179. LIPOCALIN.
PR01221. MAJORURINARY.
SUPFAMiSSF50814. SSF50814. 1 hit.
PROSITEiPS00213. LIPOCALIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02761 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLLLLLLCL GLTLVCGHAE EASSTRGNLD VAKLNGDWFS IVVASNKREK
60 70 80 90 100
IEENGSMRVF MQHIDVLENS LGFKFRIKEN GECRELYLVA YKTPEDGEYF
110 120 130 140 150
VEYDGGNTFT ILKTDYDRYV MFHLINFKNG ETFQLMVLYG RTKDLSSDIK
160 170 180
EKFAKLCEAH GITRDNIIDL TKTDRCLQAR G
Length:181
Mass (Da):20,737
Last modified:July 21, 1986 - v1
Checksum:i6A01309DC55032DC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261R → S in AAA41198. (PubMed:6167987)Curated
Sequence conflicti55 – 551G → D in CAA24531. (PubMed:6163771)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26835 mRNA. Translation: AAA40643.1.
M26837 mRNA. Translation: AAA40641.1.
AB039822 mRNA. Translation: BAA96479.1.
BC088109 mRNA. Translation: AAH88109.1.
BC098654 mRNA. Translation: AAH98654.1.
BC105816 mRNA. Translation: AAI05817.1.
U31287 mRNA. Translation: AAA75511.1.
V01220 mRNA. Translation: CAA24531.1.
J00737 mRNA. Translation: AAA41198.1.
PIRiA92317. UART.
RefSeqiNP_671747.1. NM_147214.2.
UniGeneiRn.224459.

Genome annotation databases

GeneIDi259246.
KEGGirno:259246.
UCSCiRGD:708506. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26835 mRNA. Translation: AAA40643.1 .
M26837 mRNA. Translation: AAA40641.1 .
AB039822 mRNA. Translation: BAA96479.1 .
BC088109 mRNA. Translation: AAH88109.1 .
BC098654 mRNA. Translation: AAH98654.1 .
BC105816 mRNA. Translation: AAI05817.1 .
U31287 mRNA. Translation: AAA75511.1 .
V01220 mRNA. Translation: CAA24531.1 .
J00737 mRNA. Translation: AAA41198.1 .
PIRi A92317. UART.
RefSeqi NP_671747.1. NM_147214.2.
UniGenei Rn.224459.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2A2G X-ray 2.90 A/B/C/D 1-181 [» ]
2A2U X-ray 2.50 A/B/C/D 1-181 [» ]
ProteinModelPortali P02761.
SMRi P02761. Positions 20-177.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

Allergomei 3464. Rat n 1.0101.
611. Rat n 1.

Proteomic databases

PaxDbi P02761.
PRIDEi P02761.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 259246.
KEGGi rno:259246.
UCSCi RGD:708506. rat.

Organism-specific databases

RGDi 708506. LOC259246.

Phylogenomic databases

eggNOGi NOG45000.
HOGENOMi HOG000231458.
HOVERGENi HBG000215.
InParanoidi P02761.
PhylomeDBi P02761.
TreeFami TF338197.

Miscellaneous databases

EvolutionaryTracei P02761.
NextBioi 624294.

Gene expression databases

Genevestigatori P02761.

Family and domain databases

Gene3Di 2.40.128.20. 1 hit.
InterProi IPR012674. Calycin.
IPR011038. Calycin-like.
IPR002345. Lipocalin.
IPR022272. Lipocalin_CS.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
IPR002971. Maj_urinary.
[Graphical view ]
Pfami PF00061. Lipocalin. 1 hit.
[Graphical view ]
PRINTSi PR00179. LIPOCALIN.
PR01221. MAJORURINARY.
SUPFAMi SSF50814. SSF50814. 1 hit.
PROSITEi PS00213. LIPOCALIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Length polymorphism in the 3' noncoding region of rat hepatic alpha 2u-globulin mRNAs."
    Ichiyoshi Y., Endo H., Yamamoto M.
    Biochim. Biophys. Acta 910:43-51(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Kurtz D.T., Dey M.
    Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  3. "Molecular evidence of complex tissue- and sex-specific mRNA expression of the rat alpha(2u)-globulin multigene family."
    Saito K., Nishikawa J., Imagawa M., Nishihara T., Matsuo M.
    Biochem. Biophys. Res. Commun. 272:337-344(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Preputial gland.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver and Spleen.
  5. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-181.
  6. "The structure and expression of very closely related members of the alpha 2u globulin gene family."
    Dolan K.P., Unterman R.D., McLaughlin M., Nakhasi H.L., Lynch K.R., Feigelson P.
    J. Biol. Chem. 257:13527-13534(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 34-181.
  7. "Amino acid sequence of the precursor of rat liver alpha 2 micro-globulin."
    Drickamer K., Kwoh T.J., Kurtz D.T.
    J. Biol. Chem. 256:3634-3636(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-65.
  8. "Kidney fatty acid-binding protein: identification as alpha 2U-globulin."
    Kimura H., Odani S., Suzuki J., Arakawa M., Ono T.
    FEBS Lett. 246:101-104(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 29-48.
    Tissue: Kidney.
  9. "Primary structure and cellular distribution of two fatty acid-binding proteins in adult rat kidneys."
    Kimura H., Odani S., Nishi S., Sato H., Arakawa M., Ono T.
    J. Biol. Chem. 266:5963-5972(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 29-179, DISULFIDE BOND.
    Tissue: Kidney.
  10. "Purification and identification of allergenic alpha (2u)-globulin species of rat urine."
    Bayard C., Holmquist L., Vesterberg O.
    Biochim. Biophys. Acta 1290:129-134(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 20-44, ALLERGEN.
    Tissue: Urine.
  11. Erratum
    Bayard C., Holmquist L., Vesterberg O.
    Biochim. Biophys. Acta 1291:253-255(1996)
  12. "Pheromone binding to two rodent urinary proteins revealed by X-ray crystallography."
    Boecksel Z., Groom C.R., Flower D.R., Wright C.E., Phillips S.E.V., Cavaggioni A., Findlay J.B.C., North A.C.T.
    Nature 360:186-188(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).

Entry informationi

Entry nameiMUP_RAT
AccessioniPrimary (citable) accession number: P02761
Secondary accession number(s): Q54AE7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 1, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3