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P02754

- LACB_BOVIN

UniProt

P02754 - LACB_BOVIN

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Protein

Beta-lactoglobulin

Gene
LGB
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.

GO - Molecular functioni

  1. retinol binding Source: UniProtKB-KW

GO - Biological processi

  1. transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Milk protein

Keywords - Biological processi

Transport

Keywords - Ligandi

Retinol-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactoglobulin
Short name:
Beta-LG
Alternative name(s):
Allergen: Bos d 5
Gene namesi
Name:LGB
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Is one of the causes of cow's milk allergy.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei164. Bos d 5.
2739. Bos d 5.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 16162 PublicationsAdd
BLAST
Chaini17 – 178162Beta-lactoglobulinPRO_0000017903Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi82 ↔ 1762 Publications
Disulfide bondi122 ↔ 137Alternate1 Publication
Disulfide bondi122 ↔ 1352 Publications

Post-translational modificationi

Alternate disulfide bonds occur in equal amounts in all variants examined.

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP02754.
PRIDEiP02754.

PTM databases

UniCarbKBiP02754.

Expressioni

Tissue specificityi

Synthesized in mammary gland and secreted in milk.

Interactioni

Subunit structurei

Under physiological conditions beta-lactoglobulin exists as an equilibrium mixture of monomeric and dimeric forms.

Protein-protein interaction databases

DIPiDIP-29525N.

Structurei

Secondary structure

1
178
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi19 – 213
Helixi28 – 314
Beta strandi36 – 449
Helixi45 – 473
Beta strandi48 – 503
Beta strandi57 – 648
Beta strandi70 – 789
Beta strandi81 – 9111
Beta strandi97 – 1004
Beta strandi102 – 1043
Beta strandi107 – 1137
Beta strandi115 – 12410
Beta strandi126 – 1283
Helixi129 – 1313
Beta strandi133 – 14311
Helixi146 – 15611
Beta strandi157 – 1593
Beta strandi163 – 1664
Helixi169 – 1724
Helixi175 – 1773

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1B0OX-ray2.50A17-178[»]
1B8EX-ray1.95A17-178[»]
1BEBX-ray1.80A/B17-178[»]
1BSOX-ray2.23A17-178[»]
1BSQX-ray2.22A17-178[»]
1BSYX-ray2.24A17-178[»]
1CJ5NMR-A19-178[»]
1DV9NMR-A17-178[»]
1GX8X-ray2.40A17-178[»]
1GX9X-ray2.34A17-178[»]
1GXAX-ray2.35A17-178[»]
1QG5X-ray2.00A17-178[»]
1UZ2X-ray1.95X17-178[»]
1YUPX-ray2.10D18-176[»]
2AKQX-ray3.00A/B/C/D17-178[»]
2BLGX-ray2.46A17-178[»]
2GJ5X-ray2.40A17-178[»]
2Q2MX-ray2.10A17-178[»]
2Q2PX-ray2.96A17-178[»]
2Q39X-ray2.50A/B17-178[»]
2R56X-ray2.80A/B17-178[»]
3BLGX-ray2.56A17-178[»]
3KZAX-ray2.00A/B33-169[»]
3NPOX-ray2.20A17-178[»]
3NQ3X-ray1.90A17-178[»]
3NQ9X-ray1.90A17-178[»]
3PH5X-ray2.40A/B18-178[»]
3PH6X-ray2.53A/B18-178[»]
3UEUX-ray2.10A17-178[»]
3UEVX-ray1.90A17-178[»]
3UEWX-ray2.00A17-178[»]
3UEXX-ray2.10A17-178[»]
4DQ3X-ray1.95A17-178[»]
4DQ4X-ray1.90A17-178[»]
4GNYX-ray1.64A17-178[»]
4IB6X-ray2.20A17-178[»]
4IB7X-ray2.20A17-178[»]
4IB8X-ray2.30A17-178[»]
4IB9X-ray2.20A17-178[»]
4IBAX-ray2.30A17-178[»]
4KIIX-ray1.85A17-178[»]
DisProtiDP00193.
ProteinModelPortaliP02754.
SMRiP02754. Positions 17-178.

Miscellaneous databases

EvolutionaryTraceiP02754.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG73754.
HOGENOMiHOG000113272.
HOVERGENiHBG104361.
InParanoidiP02754.

Family and domain databases

Gene3Di2.40.128.20. 1 hit.
InterProiIPR002447. Blactoglobulin.
IPR012674. Calycin.
IPR011038. Calycin-like.
IPR002345. Lipocalin.
IPR022272. Lipocalin_CS.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
[Graphical view]
PfamiPF00061. Lipocalin. 1 hit.
[Graphical view]
PRINTSiPR01172. BLCTOGLOBULN.
PR00179. LIPOCALIN.
SUPFAMiSSF50814. SSF50814. 1 hit.
PROSITEiPS00213. LIPOCALIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02754-1 [UniParc]FASTAAdd to Basket

« Hide

MKCLLLALAL TCGAQALIVT QTMKGLDIQK VAGTWYSLAM AASDISLLDA    50
QSAPLRVYVE ELKPTPEGDL EILLQKWENG ECAQKKIIAE KTKIPAVFKI 100
DALNENKVLV LDTDYKKYLL FCMENSAEPE QSLACQCLVR TPEVDDEALE 150
KFDKALKALP MHIRLSFNPT QLEEQCHI 178
Length:178
Mass (Da):19,883
Last modified:August 1, 1991 - v3
Checksum:i225F10A78C63A6B2
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti61 – 611E → Q in variant D. 1 Publication
Natural varianti72 – 721I → L in variant W. 1 Publication
Natural varianti75 – 751Q → H in variant C; found only in the Jersey breed. 1 Publication
Natural varianti80 – 801G → D in variant A. 3 Publications
Natural varianti134 – 1341A → V in variant A. 4 Publications

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti121 – 1211F → V in CAA32835. 1 Publication
Sequence conflicti136 – 1361Q → E1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14712 mRNA. Translation: CAA32835.1.
Z48305 Genomic DNA. Translation: CAA88303.1.
DQ489319 Genomic DNA. Translation: ABF48380.1.
BC108213 mRNA. Translation: AAI08214.1.
X52581 Genomic DNA. Translation: CAA36812.1.
M19088 Genomic DNA. Translation: AAA30411.1.
M27732 mRNA. Translation: AAA30412.1.
K01086 mRNA. Translation: AAA30413.1.
PIRiS10179. LGBO.
RefSeqiNP_776354.2. NM_173929.3.
UniGeneiBt.385.

Genome annotation databases

GeneIDi280838.
KEGGibta:280838.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14712 mRNA. Translation: CAA32835.1 .
Z48305 Genomic DNA. Translation: CAA88303.1 .
DQ489319 Genomic DNA. Translation: ABF48380.1 .
BC108213 mRNA. Translation: AAI08214.1 .
X52581 Genomic DNA. Translation: CAA36812.1 .
M19088 Genomic DNA. Translation: AAA30411.1 .
M27732 mRNA. Translation: AAA30412.1 .
K01086 mRNA. Translation: AAA30413.1 .
PIRi S10179. LGBO.
RefSeqi NP_776354.2. NM_173929.3.
UniGenei Bt.385.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1B0O X-ray 2.50 A 17-178 [» ]
1B8E X-ray 1.95 A 17-178 [» ]
1BEB X-ray 1.80 A/B 17-178 [» ]
1BSO X-ray 2.23 A 17-178 [» ]
1BSQ X-ray 2.22 A 17-178 [» ]
1BSY X-ray 2.24 A 17-178 [» ]
1CJ5 NMR - A 19-178 [» ]
1DV9 NMR - A 17-178 [» ]
1GX8 X-ray 2.40 A 17-178 [» ]
1GX9 X-ray 2.34 A 17-178 [» ]
1GXA X-ray 2.35 A 17-178 [» ]
1QG5 X-ray 2.00 A 17-178 [» ]
1UZ2 X-ray 1.95 X 17-178 [» ]
1YUP X-ray 2.10 D 18-176 [» ]
2AKQ X-ray 3.00 A/B/C/D 17-178 [» ]
2BLG X-ray 2.46 A 17-178 [» ]
2GJ5 X-ray 2.40 A 17-178 [» ]
2Q2M X-ray 2.10 A 17-178 [» ]
2Q2P X-ray 2.96 A 17-178 [» ]
2Q39 X-ray 2.50 A/B 17-178 [» ]
2R56 X-ray 2.80 A/B 17-178 [» ]
3BLG X-ray 2.56 A 17-178 [» ]
3KZA X-ray 2.00 A/B 33-169 [» ]
3NPO X-ray 2.20 A 17-178 [» ]
3NQ3 X-ray 1.90 A 17-178 [» ]
3NQ9 X-ray 1.90 A 17-178 [» ]
3PH5 X-ray 2.40 A/B 18-178 [» ]
3PH6 X-ray 2.53 A/B 18-178 [» ]
3UEU X-ray 2.10 A 17-178 [» ]
3UEV X-ray 1.90 A 17-178 [» ]
3UEW X-ray 2.00 A 17-178 [» ]
3UEX X-ray 2.10 A 17-178 [» ]
4DQ3 X-ray 1.95 A 17-178 [» ]
4DQ4 X-ray 1.90 A 17-178 [» ]
4GNY X-ray 1.64 A 17-178 [» ]
4IB6 X-ray 2.20 A 17-178 [» ]
4IB7 X-ray 2.20 A 17-178 [» ]
4IB8 X-ray 2.30 A 17-178 [» ]
4IB9 X-ray 2.20 A 17-178 [» ]
4IBA X-ray 2.30 A 17-178 [» ]
4KII X-ray 1.85 A 17-178 [» ]
DisProti DP00193.
ProteinModelPortali P02754.
SMRi P02754. Positions 17-178.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-29525N.

Chemistry

ChEMBLi CHEMBL1075053.

Protein family/group databases

Allergomei 164. Bos d 5.
2739. Bos d 5.0101.

PTM databases

UniCarbKBi P02754.

Proteomic databases

PaxDbi P02754.
PRIDEi P02754.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 280838.
KEGGi bta:280838.

Organism-specific databases

CTDi 5047.

Phylogenomic databases

eggNOGi NOG73754.
HOGENOMi HOG000113272.
HOVERGENi HBG104361.
InParanoidi P02754.

Miscellaneous databases

EvolutionaryTracei P02754.
NextBioi 20804987.

Family and domain databases

Gene3Di 2.40.128.20. 1 hit.
InterProi IPR002447. Blactoglobulin.
IPR012674. Calycin.
IPR011038. Calycin-like.
IPR002345. Lipocalin.
IPR022272. Lipocalin_CS.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
[Graphical view ]
Pfami PF00061. Lipocalin. 1 hit.
[Graphical view ]
PRINTSi PR01172. BLCTOGLOBULN.
PR00179. LIPOCALIN.
SUPFAMi SSF50814. SSF50814. 1 hit.
PROSITEi PS00213. LIPOCALIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ASP-80 AND VAL-134.
    Tissue: Mammary gland.
  2. "Bovine beta-lactoglobulin: cloning and expression in transgenic mice."
    Hyttinen J.M., Korhonen V.P., Myohanen S., Janne J.
    Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Sperm.
  3. "Aberrant low expression level of bovine beta-lactoglobulin B."
    Braunschweig M.H.
    Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. NIH - Mammalian Gene Collection (MGC) project
    Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.
  5. "Cloning and partial nucleotide sequence of the genomic bovine beta-lactoglobulin gene."
    Silva M.C., Wong D.W.S., Batt C.A.
    Nucleic Acids Res. 18:3051-3051(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-20.
    Tissue: Pituitary.
  6. "The sequence of beta-lactoglobulin."
    Braunitzer G., Chen R., Schrank B., Stangl A.
    Hoppe-Seyler's Z. Physiol. Chem. 354:867-878(1973) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 17-178, VARIANTS ASP-80 AND VAL-134.
  7. "The amino acid sequence of goat beta-lactoglobulin."
    Preaux G., Braunitzer G., Schrank B., Stangl A.
    Hoppe-Seyler's Z. Physiol. Chem. 360:1595-1604(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 100; 103; 171 AND 172.
  8. "Genetic variants of bovine beta-lactoglobulin. A novel wild-type beta-lactoglobulin W and its primary sequence."
    Godovac-Zimmermann J., Krause I., Buchberger J., Weiss G., Klostermeyer H.
    Biol. Chem. Hoppe-Seyler 371:255-260(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 17-178, VARIANT LEU-72.
    Strain: Murnau-Werdenfelser.
  9. "Cloning and nucleotide sequence of the bovine beta-lactoglobulin gene."
    Jamieson A.C., Vandeyar M.A., Kang Y.C., Kinsella J.E., Batt C.A.
    Gene 61:85-90(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 28-178, VARIANTS ASP-80 AND VAL-134.
  10. "Localization of the Glu-Gln substitution differentiating B and D genetic variants in the peptide chain of bovine beta lactoglobulin."
    Brignon G., Ribadeau-Dumas B.
    FEBS Lett. 33:73-76(1973) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 59-73, VARIANT GLN-61.
  11. "Molecular cloning of bovine beta-lactoglobulin cDNA."
    Ivanov V.N., Judinkova E.S., Gorodetsky S.I.
    Biol. Chem. Hoppe-Seyler 369:425-429(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 122-178.
  12. "Construction and identification by partial nucleotide sequence analysis of bovine casein and beta-lactoglobulin cDNA clones."
    Willis I.M., Stewart A.F., Caputo A., Thompson A.R., McKinlay A.G.
    DNA 1:375-386(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 125-138, VARIANT VAL-134.
  13. "Location of sulfhydryl and disulfide groups in bovine beta-lactoglobulins and effects of urea."
    McKenzie H.A., Ralston G.B., Shaw D.C.
    Biochemistry 11:4539-4547(1972) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS.
  14. Shaw D.C.
    Submitted (JAN-1973) to the PIR data bank
    Cited for: VARIANT HIS-75.
  15. "Three-dimensional structure and active site of three hydrophobic molecule-binding proteins with significant amino acid sequence similarity."
    Monaco H.L., Zanotti G.
    Biopolymers 32:457-465(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPARISON OF X-RAY STRUCTURES.
  16. "Bovine beta-lactoglobulin at 1.8-A resolution -- still an enigmatic lipocalin."
    Brownlow S., Morais-Cabral J.H., Cooper R., Flower D.R., Yewdall S.J., Polikarpov I., North A.C.T., Sawyer L.
    Structure 5:481-495(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
  17. "Structural basis of the tanford transition of bovine beta-lactoglobulin."
    Qin B.Y., Bewley M.C., Creamer L.K., Baker H.M., Baker E.N., Jameson G.B.
    Biochemistry 37:14014-14023(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.22 ANGSTROMS).
  18. "Partially folded structure of monomeric bovine beta-lactoglobulin."
    Molinari H., Ragona L., Varani L., Musco G., Consonni R., Zetta L., Monaco H.L.
    FEBS Lett. 381:237-243(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.
  19. "Solution structure and dynamics of bovine beta-lactoglobulin A."
    Kuwata K., Hoshino M., Forge V., Era S., Batt C.A., Goto Y.
    Protein Sci. 8:2541-2545(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF VARIANT A.

Entry informationi

Entry nameiLACB_BOVIN
AccessioniPrimary (citable) accession number: P02754
Secondary accession number(s): Q32P89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 1, 1991
Last modified: July 9, 2014
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The B variant sequence is shown.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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