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Protein

Beta-lactoglobulin

Gene

LGB

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.

Miscellaneous

The B variant sequence is shown.

GO - Molecular functioni

  • long-chain fatty acid binding Source: CAFA
  • retinol binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionMilk protein
Biological processTransport
LigandRetinol-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactoglobulin
Short name:
Beta-LG
Alternative name(s):
Allergen: Bos d 5
Gene namesi
Name:LGB
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Is one of the causes of cow's milk allergy.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei164. Bos d 5.
2739. Bos d 5.0101.

Chemistry databases

ChEMBLiCHEMBL1075053.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 162 PublicationsAdd BLAST16
ChainiPRO_000001790317 – 178Beta-lactoglobulinAdd BLAST162

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi82 ↔ 1762 Publications
Disulfide bondi122 ↔ 137Alternate1 Publication
Disulfide bondi122 ↔ 1352 Publications

Post-translational modificationi

Alternate disulfide bonds occur in equal amounts in all variants examined.

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP02754.
PeptideAtlasiP02754.
PRIDEiP02754.

PTM databases

UniCarbKBiP02754.

Expressioni

Tissue specificityi

Synthesized in mammary gland and secreted in milk.

Interactioni

Subunit structurei

Under physiological conditions beta-lactoglobulin exists as an equilibrium mixture of monomeric and dimeric forms.

Protein-protein interaction databases

DIPiDIP-29525N.
STRINGi9913.ENSBTAP00000019538.

Chemistry databases

BindingDBiP02754.

Structurei

Secondary structure

1178
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi19 – 21Combined sources3
Helixi28 – 31Combined sources4
Beta strandi36 – 44Combined sources9
Helixi45 – 47Combined sources3
Beta strandi48 – 50Combined sources3
Beta strandi57 – 64Combined sources8
Beta strandi66 – 68Combined sources3
Beta strandi70 – 78Combined sources9
Beta strandi81 – 91Combined sources11
Beta strandi97 – 100Combined sources4
Beta strandi102 – 104Combined sources3
Beta strandi107 – 113Combined sources7
Beta strandi115 – 124Combined sources10
Beta strandi126 – 128Combined sources3
Helixi129 – 131Combined sources3
Beta strandi133 – 143Combined sources11
Helixi146 – 156Combined sources11
Beta strandi157 – 159Combined sources3
Beta strandi163 – 166Combined sources4
Helixi169 – 172Combined sources4
Helixi175 – 177Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B0OX-ray2.50A17-178[»]
1B8EX-ray1.95A17-178[»]
1BEBX-ray1.80A/B17-178[»]
1BSOX-ray2.23A17-178[»]
1BSQX-ray2.22A17-178[»]
1BSYX-ray2.24A17-178[»]
1CJ5NMR-A19-178[»]
1DV9NMR-A17-178[»]
1GX8X-ray2.40A17-178[»]
1GX9X-ray2.34A17-178[»]
1GXAX-ray2.35A17-178[»]
1QG5X-ray2.00A17-178[»]
1UZ2X-ray1.95X17-178[»]
1YUPX-ray2.10D18-176[»]
2AKQX-ray3.00A/B/C/D17-178[»]
2BLGX-ray2.46A17-178[»]
2GJ5X-ray2.40A17-178[»]
2Q2MX-ray2.10A17-178[»]
2Q2PX-ray2.96A17-178[»]
2Q39X-ray2.50A/B17-178[»]
2R56X-ray2.80A/B17-178[»]
3BLGX-ray2.56A17-178[»]
3KZAX-ray2.00A/B33-169[»]
3NPOX-ray2.20A17-178[»]
3NQ3X-ray1.90A17-178[»]
3NQ9X-ray1.90A17-178[»]
3PH5X-ray2.40A/B18-178[»]
3PH6X-ray2.53A/B18-178[»]
3UEUX-ray2.10A17-178[»]
3UEVX-ray1.90A17-178[»]
3UEWX-ray2.00A17-178[»]
3UEXX-ray2.10A17-178[»]
4DQ3X-ray1.95A17-178[»]
4DQ4X-ray1.90A17-178[»]
4GNYX-ray1.64A17-178[»]
4IB6X-ray2.20A17-178[»]
4IB7X-ray2.20A17-178[»]
4IB8X-ray2.30A17-178[»]
4IB9X-ray2.20A17-178[»]
4IBAX-ray2.30A17-178[»]
4KIIX-ray1.85A17-178[»]
4LZUX-ray2.40A17-178[»]
4LZVX-ray2.44A17-178[»]
4Y0PX-ray2.20A17-178[»]
4Y0QX-ray2.00A17-178[»]
4Y0RX-ray2.30A17-178[»]
5EEEX-ray1.95A17-178[»]
5HTDX-ray2.50A17-178[»]
5HTEX-ray2.40A17-178[»]
5IO5X-ray2.85A17-178[»]
5IO7X-ray2.85A17-178[»]
5K06X-ray2.50A17-178[»]
5LKEX-ray2.80A17-178[»]
5LKFX-ray2.50A17-178[»]
DisProtiDP00193.
ProteinModelPortaliP02754.
SMRiP02754.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02754.

Family & Domainsi

Sequence similaritiesi

Belongs to the calycin superfamily. Lipocalin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JCG3. Eukaryota.
ENOG4111386. LUCA.
HOGENOMiHOG000113272.
HOVERGENiHBG104361.
InParanoidiP02754.

Family and domain databases

Gene3Di2.40.128.20. 1 hit.
InterProiView protein in InterPro
IPR002447. Blactoglobulin.
IPR012674. Calycin.
IPR002345. Lipocalin.
IPR022272. Lipocalin_CS.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
PfamiView protein in Pfam
PF00061. Lipocalin. 1 hit.
PRINTSiPR01172. BLCTOGLOBULN.
PR00179. LIPOCALIN.
SUPFAMiSSF50814. SSF50814. 1 hit.
PROSITEiView protein in PROSITE
PS00213. LIPOCALIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02754-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKCLLLALAL TCGAQALIVT QTMKGLDIQK VAGTWYSLAM AASDISLLDA
60 70 80 90 100
QSAPLRVYVE ELKPTPEGDL EILLQKWENG ECAQKKIIAE KTKIPAVFKI
110 120 130 140 150
DALNENKVLV LDTDYKKYLL FCMENSAEPE QSLACQCLVR TPEVDDEALE
160 170
KFDKALKALP MHIRLSFNPT QLEEQCHI
Length:178
Mass (Da):19,883
Last modified:August 1, 1991 - v3
Checksum:i225F10A78C63A6B2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti121F → V in CAA32835 (PubMed:2701948).Curated1
Sequence conflicti136Q → E (PubMed:6897774).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti61E → Q in variant D. 1 Publication1
Natural varianti72I → L in variant W. 1 Publication1
Natural varianti75Q → H in variant C; found only in the Jersey breed. 1 Publication1
Natural varianti80G → D in variant A. 3 Publications1
Natural varianti134A → V in variant A. 4 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14712 mRNA. Translation: CAA32835.1.
Z48305 Genomic DNA. Translation: CAA88303.1.
DQ489319 Genomic DNA. Translation: ABF48380.1.
BC108213 mRNA. Translation: AAI08214.1.
X52581 Genomic DNA. Translation: CAA36812.1.
M19088 Genomic DNA. Translation: AAA30411.1.
M27732 mRNA. Translation: AAA30412.1.
K01086 mRNA. Translation: AAA30413.1.
PIRiS10179. LGBO.
RefSeqiNP_776354.2. NM_173929.3.
UniGeneiBt.385.

Genome annotation databases

GeneIDi280838.
KEGGibta:280838.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiLACB_BOVIN
AccessioniPrimary (citable) accession number: P02754
Secondary accession number(s): Q32P89
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 1, 1991
Last modified: May 10, 2017
This is version 150 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families