P02742 (CRP_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-reactive protein

Gene names

Name:	CRP
Synonyms:	PTX1

Organism

Oryctolagus cuniculus (Rabbit) [Reference proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells By similarity.
Cofactor	Binds 2 calcium ions per subunit By similarity.
Subunit structure	Homopentamer. Pentaxin (or pentraxin) have a discoid arrangement of 5 non-covalently bound subunits By similarity.
Subcellular location	Secreted.
Tissue specificity	Found in plasma.
Induction	The concentration of CRP in plasma increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli.
Miscellaneous	Asp-61, Arg-76, Arg-77, and Glu-81 may be involved in the calcium-dependent binding of phosphorylcholine, a property that may be important for the biological function of this protein.
Sequence similarities	Belongs to the pentaxin family. Contains 1 pentaxin domain.

Ontologies

Keywords
Biological process	Acute phase
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	acute-phase response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Ref.3
	Chain	21 – 225	205	C-reactive protein Ref.3		PRO_0000023531
Regions
	Domain	21 – 225	205	Pentaxin
Sites
	Metal binding	80	1	Calcium 1 By similarity
	Metal binding	158	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	159	1	Calcium 1 By similarity
	Metal binding	159	1	Calcium 2 By similarity
	Metal binding	169	1	Calcium 2 By similarity
Amino acid modifications
	Disulfide bond	55 ↔ 116		Ref.3
Natural variations
	Natural variant	62	1	L → K.
	Natural variant	89	1	D → V.
Experimental info
	Sequence conflict	10	1	T → I in AAA31206. Ref.2
	Sequence conflict	46	1	K → T AA sequence Ref.3
	Sequence conflict	50	1	K → L AA sequence Ref.3
	Sequence conflict	76	1	R → K in AAA75404. Ref.1
	Sequence conflict	84 – 88	5	LFWSK → FFVKE AA sequence Ref.3
	Sequence conflict	91	1	G → V AA sequence Ref.3
	Sequence conflict	93 – 101	9	Missing AA sequence Ref.3
	Sequence conflict	108	1	I → V in AAA31206. Ref.2
	Sequence conflict	167	1	K → W AA sequence Ref.3
	Sequence conflict	177	1	N → D AA sequence Ref.3
	Sequence conflict	193	1	I → V in AAA31206. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P02742 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: C6D634FDE844438F	FASTA	225	25,493
	10 20 30 40 50 60 MEKLLWCFLT LVSFSNMSDQ AGMHKKAFVF PKESDNSYVS LNAQLKKPLK AFTVCLYFYT 70 80 90 100 110 120 DLSMTRGYSI FSYATRRQFN EILLFWSKDI GYSFSVGGDE IIFKVSDIPV DPTHLCASWE 130 140 150 160 170 180 SSTGIAELWV DGKPMVRKSL KKGYILGPEA SIILGQDQDS FGGSFEKQQS LVGDIGNVNM 190 200 210 220 WDYALSPEEI NTIYAGGTFS PNVLDWRELT YQVRGEVHVK PQLWP

References

[1]	"Cloning and characterization of the gene for rabbit C-reactive protein." Hu S.-I., Miller S.M., Samols D. Biochemistry 25:7834-7839(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: New Zealand white. Tissue: Liver.
[2]	"Rabbit C-reactive protein. Biosynthesis and characterization of cDNA clones." Syin C., Gotschlich E.C., Liu T.-Y. J. Biol. Chem. 261:5473-5479(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Primary structure of rabbit C-reactive protein." Wang C.-M., Nguyen N.Y., Yonaha K., Robey F., Liu T.-Y. J. Biol. Chem. 257:13610-13615(1982) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-225.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

No more Christmas pudding? - Issue 30 of January 2003

Cross-references

Sequence databases
EMBL GenBank DDBJ	M14538 Genomic DNA. Translation: AAA75403.1. L47237 mRNA. Translation: AAA75404.1. M13497 mRNA. Translation: AAA31206.1.
PIR	CJRB. A25605.
RefSeq	NP_001075734.1. NM_001082265.1.
UniGene	Ocu.1848.
3D structure databases
ProteinModelPortal	P02742.
SMR	P02742. Positions 20-225.
ModBase	Search...
Protein-protein interaction databases
STRING	9986.ENSOCUP00000004059.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100009091.
Organism-specific databases
CTD	1401.
Phylogenomic databases
eggNOG	NOG113288.
HOGENOM	HOG000247043.
HOVERGEN	HBG005405.
OrthoDB	EOG4R23VS.
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR001759. Pentaxin. [Graphical view]
Pfam	PF00354. Pentaxin. 1 hit. [Graphical view]
PRINTS	PR00895. PENTAXIN.
SMART	SM00159. PTX. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS00289. PENTAXIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CRP_RABIT

Accession

Primary (citable) accession number: P02742

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 1, 1988
Last modified:	April 3, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program