Reviewed, UniProtKB/Swiss-Prot P02742 (CRP_RABIT)

Names and origin

Protein names

Recommended name:
    C-reactive protein

Gene names

Name:	CRP
Synonyms:	PTX1

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells By similarity.
Cofactor	Binds 2 calcium ions per subunit By similarity.
Subunit structure	Homopentamer. Pentaxin (or pentraxin) have a discoid arrangement of 5 non-covalently bound subunits By similarity.
Subcellular location	Secreted.
Tissue specificity	Found in plasma.
Induction	The concentration of CRP in plasma increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli.
Miscellaneous	Asp-61, Arg-76, Arg-77, and Glu-81 may be involved in the calcium-dependent binding of phosphorylcholine, a property that may be important for the biological function of this protein.
Sequence similarities	Belongs to the pentaxin family. Contains 1 pentaxin domain.

Ontologies

Keywords
Biological process	Acute phase
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	acute-phase response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Ref.3
	Chain	21 – 225	205	C-reactive protein Ref.3		PRO_0000023531
Regions
	Domain	21 – 225	205	Pentaxin
Sites
	Metal binding	80	1	Calcium 1 By similarity
	Metal binding	158	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	159	1	Calcium 1 By similarity
	Metal binding	159	1	Calcium 2 By similarity
	Metal binding	169	1	Calcium 2 By similarity
Amino acid modifications
	Disulfide bond	55 ↔ 116		Ref.3
Natural variations
	Natural variant	62	1	L → K
	Natural variant	89	1	D → V
Experimental info
	Sequence conflict	10	1	T → I in AAA31206. Ref.2
	Sequence conflict	46	1	K → T AA sequence Ref.3
	Sequence conflict	50	1	K → L AA sequence Ref.3
	Sequence conflict	76	1	R → K in AAA75404. Ref.1
	Sequence conflict	84 – 88	5	LFWSK → FFVKE AA sequence Ref.3
	Sequence conflict	91	1	G → V AA sequence Ref.3
	Sequence conflict	93 – 101	9	Missing AA sequence Ref.3
	Sequence conflict	108	1	I → V in AAA31206. Ref.2
	Sequence conflict	167	1	K → W AA sequence Ref.3
	Sequence conflict	177	1	N → D AA sequence Ref.3
	Sequence conflict	193	1	I → V in AAA31206. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P02742-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: C6D634FDE844438F	FASTA	225	25,493
	10 20 30 40 50 60 MEKLLWCFLT LVSFSNMSDQ AGMHKKAFVF PKESDNSYVS LNAQLKKPLK AFTVCLYFYT 70 80 90 100 110 120 DLSMTRGYSI FSYATRRQFN EILLFWSKDI GYSFSVGGDE IIFKVSDIPV DPTHLCASWE 130 140 150 160 170 180 SSTGIAELWV DGKPMVRKSL KKGYILGPEA SIILGQDQDS FGGSFEKQQS LVGDIGNVNM 190 200 210 220 WDYALSPEEI NTIYAGGTFS PNVLDWRELT YQVRGEVHVK PQLWP

References

[1]	"Cloning and characterization of the gene for rabbit C-reactive protein." Hu S.-I., Miller S.M., Samols D. Biochemistry 25:7834-7839(1986) [PubMed: 3026463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: New Zealand white. Tissue: Liver.
[2]	"Rabbit C-reactive protein. Biosynthesis and characterization of cDNA clones." Syin C., Gotschlich E.C., Liu T.-Y. J. Biol. Chem. 261:5473-5479(1986) [PubMed: 3007506] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Primary structure of rabbit C-reactive protein." Wang C.-M., Nguyen N.Y., Yonaha K., Robey F., Liu T.-Y. J. Biol. Chem. 257:13610-13615(1982) [PubMed: 6754715] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-225.

Web resources

Protein Spotlight

No more Christmas pudding? - Issue 30 of January 2003

Cross-references

Sequence databases
EMBLGenBankDDBJ	M14538 Genomic DNA. Translation: AAA75403.1. L47237 mRNA. Translation: AAA75404.1. M13497 mRNA. Translation: AAA31206.1.
PIR	CJRB. A25605.
RefSeq	NP_001075734.1.
UniGene	Ocu.1848
3D structure databases
HSSP	HSSP built from PDB template 1LJ7 based on UniProtKB P02741.
SMR	P02742. Positions 20-225.
ModBase	Search...
Genome annotation databases
Ensembl	ENSOCUG00000004702. Oryctolagus cuniculus. [Contig view]
GeneID	100009091.
Phylogenomic databases
HOVERGEN	P02742.
Family and domain databases
InterPro	IPR013320. ConA-like_subgrp. IPR001759. Pentaxin. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.
Pfam	PF00354. Pentaxin. 1 hit. [Graphical view]
PRINTS	PR00895. PENTAXIN.
ProDom	PD002153. Pentaxin. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00159. PTX. 1 hit. [Graphical view]
PROSITE	PS00289. PENTAXIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CRP_RABIT

Accession

Primary (citable) accession number: P02742

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 1, 1988
Last modified:	June 16, 2009
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)