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Protein

Asialoglycoprotein receptor 1

Gene

Asgr1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi190 – 1901Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi196 – 1961Calcium 1By similarity
Metal bindingi215 – 2151Calcium 2By similarity
Metal bindingi239 – 2391Calcium 3By similarity
Binding sitei239 – 2391CarbohydrateBy similarity
Metal bindingi241 – 2411Calcium 3By similarity
Metal bindingi242 – 2421Calcium 2By similarity
Binding sitei243 – 2431CarbohydrateBy similarity
Metal bindingi252 – 2521Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi252 – 2521Calcium 3By similarity
Binding sitei252 – 2521CarbohydrateBy similarity
Metal bindingi253 – 2531Calcium 2By similarity
Metal bindingi264 – 2641Calcium 3By similarity
Metal bindingi265 – 2651Calcium 3By similarity
Metal bindingi277 – 2771Calcium 1By similarity

GO - Molecular functioni

  • asialoglycoprotein receptor activity Source: RGD
  • carbohydrate binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellular response to extracellular stimulus Source: Ensembl
  • receptor-mediated endocytosis Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Calcium, Lectin, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Asialoglycoprotein receptor 1
Short name:
ASGP-R 1
Short name:
ASGPR 1
Alternative name(s):
Hepatic lectin 1
Short name:
HL-1
Short name:
rHL-1
Gene namesi
Name:Asgr1
Synonyms:Asgr-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi2160. Asgr1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3939CytoplasmicSequence analysisAdd
BLAST
Transmembranei40 – 6021Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini61 – 284224ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 284284Asialoglycoprotein receptor 1PRO_0000046653Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi35 – 351S-palmitoyl cysteine1 Publication
Glycosylationi75 – 751N-linked (GlcNAc...)Sequence analysis
Glycosylationi78 – 781N-linked (GlcNAc...)Sequence analysis
Glycosylationi146 – 1461N-linked (GlcNAc...)Sequence analysis
Disulfide bondi153 ↔ 164PROSITE-ProRule annotation
Disulfide bondi181 ↔ 276PROSITE-ProRule annotation
Disulfide bondi254 ↔ 268PROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated on a cytoplasmic Ser residue.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP02706.
PRIDEiP02706.

PTM databases

iPTMnetiP02706.
SwissPalmiP02706.

Expressioni

Tissue specificityi

Expressed exclusively in hepatic parenchymal cells.

Gene expression databases

GenevisibleiP02706. RN.

Interactioni

Subunit structurei

Interacts with LASS2.By similarity

Protein-protein interaction databases

MINTiMINT-4585792.
STRINGi10116.ENSRNOP00000025254.

Structurei

3D structure databases

ProteinModelPortaliP02706.
SMRiP02706. Positions 153-280.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini160 – 277118C-type lectinPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili58 – 12265Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi5 – 84Endocytosis signalSequence analysis

Sequence similaritiesi

Contains 1 C-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00760000118924.
HOGENOMiHOG000034093.
HOVERGENiHBG000270.
InParanoidiP02706.
KOiK10063.
OMAiQHHMGPV.
OrthoDBiEOG7XDBGR.
PhylomeDBiP02706.
TreeFamiTF352155.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR005640. Lectin_N.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
PF03954. Lectin_N. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P02706-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKDYQDFQH LDNENDHHQL QRGPPPAPRL LQRLCSGFRL FLLSLGLSIL
60 70 80 90 100
LLVVVCVITS QNSQLREDLR VLRQNFSNFT VSTEDQVKAL TTQGERVGRK
110 120 130 140 150
MKLVESQLEK HQEDLREDHS RLLLHVKQLV SDVRSLSCQM AALRGNGSER
160 170 180 190 200
ICCPINWVEY EGSCYWFSSS VKPWTEADKY CQLENAHLVV VTSWEEQRFV
210 220 230 240 250
QQHMGPLNTW IGLTDQNGPW KWVDGTDYET GFKNWRPGQP DDWYGHGLGG
260 270 280
GEDCAHFTTD GHWNDDVCRR PYRWVCETEL GKAN
Length:284
Mass (Da):32,849
Last modified:January 23, 2007 - v2
Checksum:i5D973A09458900BA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti61 – 611Q → R (PubMed:6095287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02817 Genomic DNA. Translation: AAA42037.1.
BC088154 mRNA. Translation: AAH88154.1.
M21770 mRNA. Translation: AAA40764.1.
PIRiA92497. LNRTL.
RefSeqiNP_036635.1. NM_012503.2.
XP_006246641.1. XM_006246579.1.
UniGeneiRn.44300.

Genome annotation databases

EnsembliENSRNOT00000025254; ENSRNOP00000025254; ENSRNOG00000018693.
ENSRNOT00000086919; ENSRNOP00000070316; ENSRNOG00000018693.
GeneIDi24210.
KEGGirno:24210.
UCSCiRGD:2160. rat.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

Hepatic asialoglycoprotein receptor subunit 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02817 Genomic DNA. Translation: AAA42037.1.
BC088154 mRNA. Translation: AAH88154.1.
M21770 mRNA. Translation: AAA40764.1.
PIRiA92497. LNRTL.
RefSeqiNP_036635.1. NM_012503.2.
XP_006246641.1. XM_006246579.1.
UniGeneiRn.44300.

3D structure databases

ProteinModelPortaliP02706.
SMRiP02706. Positions 153-280.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4585792.
STRINGi10116.ENSRNOP00000025254.

PTM databases

iPTMnetiP02706.
SwissPalmiP02706.

Proteomic databases

PaxDbiP02706.
PRIDEiP02706.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025254; ENSRNOP00000025254; ENSRNOG00000018693.
ENSRNOT00000086919; ENSRNOP00000070316; ENSRNOG00000018693.
GeneIDi24210.
KEGGirno:24210.
UCSCiRGD:2160. rat.

Organism-specific databases

CTDi432.
RGDi2160. Asgr1.

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00760000118924.
HOGENOMiHOG000034093.
HOVERGENiHBG000270.
InParanoidiP02706.
KOiK10063.
OMAiQHHMGPV.
OrthoDBiEOG7XDBGR.
PhylomeDBiP02706.
TreeFamiTF352155.

Miscellaneous databases

PROiP02706.

Gene expression databases

GenevisibleiP02706. RN.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR005640. Lectin_N.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
PF03954. Lectin_N. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Rat liver asialoglycoprotein receptor lacks a cleavable NH2-terminal signal sequence."
    Holland E.C., Leung J.O., Drickamer K.
    Proc. Natl. Acad. Sci. U.S.A. 81:7338-7342(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Characterization of the gene encoding the major rat liver asialoglycoprotein receptor."
    Leung J.O., Holland E.C., Drickamer K.
    J. Biol. Chem. 260:12523-12527(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  4. "Isolation and expression of cDNA clones for a rat liver asialoglycoprotein receptor."
    Watts C.
    Biosci. Rep. 6:527-534(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 12-284.
  5. "Fatty acylation of the rat and human asialoglycoprotein receptors. A conserved cytoplasmic cysteine residue is acylated in all receptor subunits."
    Zeng F.Y., Weigel P.H.
    J. Biol. Chem. 271:32454-32460(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-35.

Entry informationi

Entry nameiASGR1_RAT
AccessioniPrimary (citable) accession number: P02706
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Calcium is required for ligand binding.
Two types of rat hepatic lectin have been identified, RHL-1 and RHL-2/3, having a relative abundance of 4:1.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.