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P02701

- AVID_CHICK

UniProt

P02701 - AVID_CHICK

Protein

Avidin

Gene

AVD

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei57 – 571Biotin

    Keywords - Ligandi

    Biotin

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-5341.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Avidin
    Gene namesi
    Name:AVD
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Chromosome Z

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 24242 PublicationsAdd
    BLAST
    Chaini25 – 152128AvidinPRO_0000002722Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi28 ↔ 107
    Glycosylationi41 – 411N-linked (GlcNAc...)1 PublicationCAR_000230

    Post-translational modificationi

    N-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)][Man(alpha1-?)Man(alpha1-6)] Man(beta1-4)GlcNAc(beta1-4)GlcNAc.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP02701.
    PRIDEiP02701.

    PTM databases

    UniCarbKBiP02701.

    Expressioni

    Tissue specificityi

    Synthesized in hen oviduct and concentrated in egg white (where it represent 0.05% of the total protein).

    Interactioni

    Subunit structurei

    Homotetramer.

    Protein-protein interaction databases

    DIPiDIP-60471N.

    Structurei

    Secondary structure

    1
    152
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi32 – 365
    Beta strandi41 – 444
    Beta strandi51 – 588
    Beta strandi63 – 664
    Beta strandi71 – 777
    Helixi80 – 823
    Beta strandi87 – 937
    Beta strandi95 – 984
    Beta strandi100 – 10910
    Beta strandi111 – 1133
    Beta strandi115 – 12410
    Helixi130 – 1356
    Beta strandi137 – 14610

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AVDX-ray2.70A/B25-152[»]
    1AVEX-ray2.80A/B25-152[»]
    1IJ8X-ray2.00A/B25-152[»]
    1LDOX-ray2.20A/B25-152[»]
    1LDQX-ray2.70A/B25-152[»]
    1LELX-ray2.90A/B25-152[»]
    1NQNX-ray1.80A/B26-147[»]
    1RAVX-ray2.20A/B26-152[»]
    1VYOX-ray1.48A/B25-152[»]
    2A5BX-ray2.49A/B25-148[»]
    2A5CX-ray2.50A/B25-147[»]
    2A8GX-ray1.99A/B27-152[»]
    2AVIX-ray3.00A/B25-152[»]
    2C4IX-ray1.95A22-80[»]
    A99-147[»]
    2CAMX-ray2.20A/B26-152[»]
    2JGSX-ray1.90A/B/C/D69-152[»]
    3FDCX-ray3.10A/B25-152[»]
    3MM0X-ray2.70A/B/C/D/E/F/G/H/I/K/M/N25-61[»]
    A/B/C/D/E/F/G/H/I/K/M/N85-152[»]
    3VGWX-ray1.60A/B/C/D/E/F/G/H25-147[»]
    3VHHX-ray2.26A/B/C/D25-147[»]
    3VHIX-ray1.76A/B/C/D26-147[»]
    3VHMX-ray2.00A/B/C/D25-147[»]
    4I60X-ray2.50A25-152[»]
    4JHQX-ray1.99A/B25-152[»]
    ProteinModelPortaliP02701.
    SMRiP02701. Positions 26-147.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP02701.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini26 – 149124Avidin-likePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the avidin/streptavidin family.Curated
    Contains 1 avidin-like domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG316013.
    GeneTreeiENSGT00390000001847.
    HOGENOMiHOG000034237.
    HOVERGENiHBG004323.
    OrthoDBiEOG7PGDT6.
    PhylomeDBiP02701.
    TreeFamiTF336756.

    Family and domain databases

    Gene3Di2.40.128.30. 1 hit.
    InterProiIPR005469. Avidin.
    IPR017889. Avidin-like_CS.
    IPR005468. Avidin/str.
    [Graphical view]
    PfamiPF01382. Avidin. 1 hit.
    [Graphical view]
    PRINTSiPR00709. AVIDIN.
    SUPFAMiSSF50876. SSF50876. 1 hit.
    PROSITEiPS00577. AVIDIN_1. 1 hit.
    PS51326. AVIDIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P02701-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVHATSPLLL LLLLSLALVA PGLSARKCSL TGKWTNDLGS NMTIGAVNSR    50
    GEFTGTYITA VTATSNEIKE SPLHGTQNTI NKRTQPTFGF TVNWKFSEST 100
    TVFTGQCFID RNGKEVLKTM WLLRSSVNDI GDDWKATRVG INIFTRLRTQ 150
    KE 152
    Length:152
    Mass (Da):16,768
    Last modified:September 11, 2007 - v3
    Checksum:i7795A458481AEC09
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti22 – 221G → S in AAB59733. (PubMed:7665080)Curated
    Sequence conflicti35 – 351T → D in CAC34569. (PubMed:11167523)Curated
    Sequence conflicti50 – 501R → K in CAC34569. (PubMed:11167523)Curated
    Sequence conflicti77 – 771Q → E AA sequence (PubMed:5100763)Curated
    Sequence conflicti77 – 771Q → E AA sequence (PubMed:5100762)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti58 – 581I → T in about 50% of the molecules. 2 Publications

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05343 mRNA. Translation: CAA28954.1.
    L27818 Genomic DNA. Translation: AAB59733.1.
    AJ311647 Genomic DNA. Translation: CAC34569.1.
    PIRiA54975. VICH.
    RefSeqiNP_990651.1. NM_205320.1.
    UniGeneiGga.729.

    Genome annotation databases

    EnsembliENSGALT00000003855; ENSGALP00000003846; ENSGALG00000023622.
    GeneIDi396260.
    KEGGigga:396260.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    Worthington enzyme manual

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05343 mRNA. Translation: CAA28954.1 .
    L27818 Genomic DNA. Translation: AAB59733.1 .
    AJ311647 Genomic DNA. Translation: CAC34569.1 .
    PIRi A54975. VICH.
    RefSeqi NP_990651.1. NM_205320.1.
    UniGenei Gga.729.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AVD X-ray 2.70 A/B 25-152 [» ]
    1AVE X-ray 2.80 A/B 25-152 [» ]
    1IJ8 X-ray 2.00 A/B 25-152 [» ]
    1LDO X-ray 2.20 A/B 25-152 [» ]
    1LDQ X-ray 2.70 A/B 25-152 [» ]
    1LEL X-ray 2.90 A/B 25-152 [» ]
    1NQN X-ray 1.80 A/B 26-147 [» ]
    1RAV X-ray 2.20 A/B 26-152 [» ]
    1VYO X-ray 1.48 A/B 25-152 [» ]
    2A5B X-ray 2.49 A/B 25-148 [» ]
    2A5C X-ray 2.50 A/B 25-147 [» ]
    2A8G X-ray 1.99 A/B 27-152 [» ]
    2AVI X-ray 3.00 A/B 25-152 [» ]
    2C4I X-ray 1.95 A 22-80 [» ]
    A 99-147 [» ]
    2CAM X-ray 2.20 A/B 26-152 [» ]
    2JGS X-ray 1.90 A/B/C/D 69-152 [» ]
    3FDC X-ray 3.10 A/B 25-152 [» ]
    3MM0 X-ray 2.70 A/B/C/D/E/F/G/H/I/K/M/N 25-61 [» ]
    A/B/C/D/E/F/G/H/I/K/M/N 85-152 [» ]
    3VGW X-ray 1.60 A/B/C/D/E/F/G/H 25-147 [» ]
    3VHH X-ray 2.26 A/B/C/D 25-147 [» ]
    3VHI X-ray 1.76 A/B/C/D 26-147 [» ]
    3VHM X-ray 2.00 A/B/C/D 25-147 [» ]
    4I60 X-ray 2.50 A 25-152 [» ]
    4JHQ X-ray 1.99 A/B 25-152 [» ]
    ProteinModelPortali P02701.
    SMRi P02701. Positions 26-147.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60471N.

    Chemistry

    ChEMBLi CHEMBL2189156.

    PTM databases

    UniCarbKBi P02701.

    Proteomic databases

    PaxDbi P02701.
    PRIDEi P02701.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSGALT00000003855 ; ENSGALP00000003846 ; ENSGALG00000023622 .
    GeneIDi 396260.
    KEGGi gga:396260.

    Organism-specific databases

    CTDi 567678.

    Phylogenomic databases

    eggNOGi NOG316013.
    GeneTreei ENSGT00390000001847.
    HOGENOMi HOG000034237.
    HOVERGENi HBG004323.
    OrthoDBi EOG7PGDT6.
    PhylomeDBi P02701.
    TreeFami TF336756.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-5341.

    Miscellaneous databases

    EvolutionaryTracei P02701.
    NextBioi 20816312.

    Family and domain databases

    Gene3Di 2.40.128.30. 1 hit.
    InterProi IPR005469. Avidin.
    IPR017889. Avidin-like_CS.
    IPR005468. Avidin/str.
    [Graphical view ]
    Pfami PF01382. Avidin. 1 hit.
    [Graphical view ]
    PRINTSi PR00709. AVIDIN.
    SUPFAMi SSF50876. SSF50876. 1 hit.
    PROSITEi PS00577. AVIDIN_1. 1 hit.
    PS51326. AVIDIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cloning and expression of avidin in Escherichia coli."
      Chandra G., Gray J.G.
      Methods Enzymol. 184:70-79(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Cloning and sequencing of the chicken egg-white avidin-encoding gene and its relationship with the avidin-related genes Avr1-Avr5."
      Wallen M.J., Laukkanen M.O., Kulomaa M.S.
      Gene 161:205-209(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: White leghorn.
      Tissue: Oviduct.
    4. "Characterization and chromosomal localization of the chicken avidin gene family."
      Ahlroth M.K., Kola E.H., Ewald D., Masabanda J., Sazanov A., Fries R., Kulomaa M.S.
      Anim. Genet. 31:367-375(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-58.
    5. "Egg white avidin. 3. Sequence of the 78-residue middle cyanogen bromide peptide. Complete amino acid sequence of the protein subunit."
      Delange R.J., Huang T.-S.
      J. Biol. Chem. 246:698-709(1971) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-152, GLYCOSYLATION AT ASN-41.
    6. "Egg white avidin. II. Isolation, composition, and amino acid sequences of the tryptic peptides."
      Huang T.-S., DeLange R.J.
      J. Biol. Chem. 246:686-697(1971) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-152, VARIANT THR-58.
    7. "Studies on the biotin-binding sites of avidin and streptavidin. Tyrosine residues are involved in the binding site."
      Gitlin G., Bayer E.A., Wilchek M.
      Biochem. J. 269:527-530(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: IMPORTANCE OF TYR IN BIOTIN-BINDING.
    8. "Studies on the biotin-binding site of avidin. Minimized fragments that bind biotin."
      Hiller Y., Bayer E.A., Wilchek M.
      Biochem. J. 278:573-585(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOTIN-BINDING STUDIES.
    9. "Compositional and structural heterogeneity of avidin glycopeptides."
      Bruch R.C., White H.B. III
      Biochemistry 21:5334-5341(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE OF CARBOHYDRATE.
    10. "Three-dimensional structures of avidin and the avidin-biotin complex."
      Livnah O., Bayer E.A., Wilchek M., Sussman J.L.
      Proc. Natl. Acad. Sci. U.S.A. 90:5076-5080(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
    11. "Three-dimensional structure of the tetragonal crystal form of egg-white avidin in its functional complex with biotin at 2.7-A resolution."
      Pugliese L., Coda A., Malcovati M., Bolognesi M.
      J. Mol. Biol. 231:698-710(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
    12. "Biochemical characterization and crystal structure of a recombinant hen avidin and its acidic mutant expressed in Escherichia coli."
      Nardone E., Rosano C., Santambrogio P., Curnis F., Corti A., Magni F., Siccardi A.G., Paganelli G., Losso R., Apreda B., Bolognesi M., Sidoli A., Arosio P.
      Eur. J. Biochem. 256:453-460(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).

    Entry informationi

    Entry nameiAVID_CHICK
    AccessioniPrimary (citable) accession number: P02701
    Secondary accession number(s): Q91958, Q98SH4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: September 11, 2007
    Last modified: October 1, 2014
    This is version 131 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3