P02701 (AVID_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Avidin

Gene names

Name:

AVD

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
Subunit structure	Homotetramer.
Subcellular location	Secreted.
Tissue specificity	Synthesized in hen oviduct and concentrated in egg white (where it represent 0.05% of the total protein).
Post-translational modification	N-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)][Man(alpha1-?)Man(alpha1-6)] Man(beta1-4)GlcNAc(beta1-4)GlcNAc.
Sequence similarities	Belongs to the avidin/streptavidin family. Contains 1 avidin-like domain.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Ligand	Biotin
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

24

Ref.5 Ref.6

Chain

25 – 152

128

Avidin

PRO_0000002722

Regions

Domain

26 – 149

124

Avidin-like

Sites

Binding site

57

1

Biotin

Amino acid modifications

Glycosylation

41

1

N-linked (GlcNAc...) Ref.5

CAR_000230

Disulfide bond

28 ↔ 107

Natural variations

Natural variant

58

1

I → T in about 50% of the molecules. Ref.4 Ref.6

Experimental info

Sequence conflict

22

1

G → S in AAB59733. Ref.3

Sequence conflict

35

1

T → D in CAC34569. Ref.4

Sequence conflict

50

1

R → K in CAC34569. Ref.4

Sequence conflict

77

1

Q → E AA sequence Ref.5

Sequence conflict

77

1

Q → E AA sequence Ref.6

Secondary structure

1

.

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152

Helix Strand Turn

Details...

Beta strand

32 – 36

5

Beta strand

41 – 44

4

Beta strand

51 – 58

8

Beta strand

63 – 66

4

Beta strand

71 – 77

7

Helix

80 – 82

3

Beta strand

87 – 93

7

Beta strand

95 – 98

4

Beta strand

100 – 109

10

Beta strand

111 – 113

3

Beta strand

115 – 124

10

Helix

130 – 135

6

Beta strand

137 – 146

10

Sequences

	Sequence		Length	Mass (Da)
	P02701 [UniParc]. Last modified September 11, 2007. Version 3. Checksum: 7795A458481AEC09	FASTA	152	16,768
	10 20 30 40 50 60 MVHATSPLLL LLLLSLALVA PGLSARKCSL TGKWTNDLGS NMTIGAVNSR GEFTGTYITA 70 80 90 100 110 120 VTATSNEIKE SPLHGTQNTI NKRTQPTFGF TVNWKFSEST TVFTGQCFID RNGKEVLKTM 130 140 150 WLLRSSVNDI GDDWKATRVG INIFTRLRTQ KE

References

[1]	"Molecular cloning of the chicken avidin cDNA." Gope M.L., Keinaenen R.A., Kristo P.A., Conneely O.M., Beatie W.G., Zarucki-Schulz T., O'Malley B.W., Kulomaa M.S. Nucleic Acids Res. 15:3595-3606(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning and expression of avidin in Escherichia coli." Chandra G., Gray J.G. Methods Enzymol. 184:70-79(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Cloning and sequencing of the chicken egg-white avidin-encoding gene and its relationship with the avidin-related genes Avr1-Avr5." Wallen M.J., Laukkanen M.O., Kulomaa M.S. Gene 161:205-209(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: White leghorn. Tissue: Oviduct.
[4]	"Characterization and chromosomal localization of the chicken avidin gene family." Ahlroth M.K., Kola E.H., Ewald D., Masabanda J., Sazanov A., Fries R., Kulomaa M.S. Anim. Genet. 31:367-375(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-58.
[5]	"Egg white avidin. 3. Sequence of the 78-residue middle cyanogen bromide peptide. Complete amino acid sequence of the protein subunit." Delange R.J., Huang T.-S. J. Biol. Chem. 246:698-709(1971) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-152, GLYCOSYLATION AT ASN-41.
[6]	"Egg white avidin. II. Isolation, composition, and amino acid sequences of the tryptic peptides." Huang T.-S., DeLange R.J. J. Biol. Chem. 246:686-697(1971) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-152, VARIANT THR-58.
[7]	"Studies on the biotin-binding sites of avidin and streptavidin. Tyrosine residues are involved in the binding site." Gitlin G., Bayer E.A., Wilchek M. Biochem. J. 269:527-530(1990) [PubMed] [Europe PMC] [Abstract] Cited for: IMPORTANCE OF TYR IN BIOTIN-BINDING.
[8]	"Studies on the biotin-binding site of avidin. Minimized fragments that bind biotin." Hiller Y., Bayer E.A., Wilchek M. Biochem. J. 278:573-585(1991) [PubMed] [Europe PMC] [Abstract] Cited for: BIOTIN-BINDING STUDIES.
[9]	"Compositional and structural heterogeneity of avidin glycopeptides." Bruch R.C., White H.B. III Biochemistry 21:5334-5341(1982) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE OF CARBOHYDRATE.
[10]	"Three-dimensional structures of avidin and the avidin-biotin complex." Livnah O., Bayer E.A., Wilchek M., Sussman J.L. Proc. Natl. Acad. Sci. U.S.A. 90:5076-5080(1993) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
[11]	"Three-dimensional structure of the tetragonal crystal form of egg-white avidin in its functional complex with biotin at 2.7-A resolution." Pugliese L., Coda A., Malcovati M., Bolognesi M. J. Mol. Biol. 231:698-710(1993) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
[12]	"Biochemical characterization and crystal structure of a recombinant hen avidin and its acidic mutant expressed in Escherichia coli." Nardone E., Rosano C., Santambrogio P., Curnis F., Corti A., Magni F., Siccardi A.G., Paganelli G., Losso R., Apreda B., Bolognesi M., Sidoli A., Arosio P. Eur. J. Biochem. 256:453-460(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
+	Additional computationally mapped references.

Web resources

Worthington enzyme manual

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X05343 mRNA. Translation: CAA28954.1.
L27818 Genomic DNA. Translation: AAB59733.1.
AJ311647 Genomic DNA. Translation: CAC34569.1.

IPI

IPI00601265.

PIR

VICH. A54975.

RefSeq

NP_990651.1. NM_205320.1.

UniGene

Gga.729.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AVD	X-ray	2.70	A/B	25-152	[»]
1AVE	X-ray	2.80	A/B	25-152	[»]
1IJ8	X-ray	2.00	A/B	25-152	[»]
1LDO	X-ray	2.20	A/B	25-152	[»]
1LDQ	X-ray	2.70	A/B	25-152	[»]
1LEL	X-ray	2.90	A/B	25-152	[»]
1NQN	X-ray	1.80	A/B	26-147	[»]
1RAV	X-ray	2.20	A/B	26-152	[»]
1VYO	X-ray	1.48	A/B	25-152	[»]
2A5B	X-ray	2.49	A/B	25-148	[»]
2A5C	X-ray	2.50	A/B	25-147	[»]
2A8G	X-ray	1.99	A/B	27-152	[»]
2AVI	X-ray	3.00	A/B	25-152	[»]
2C4I	X-ray	1.95	A	27-147	[»]
2CAM	X-ray	2.20	A/B	28-152	[»]
2JGS	X-ray	1.90	A/B/C/D	24-152	[»]
3FDC	X-ray	3.10	A/B	25-152	[»]
3MM0	X-ray	2.70	A/B/C/D/E/F/G/H/I/K/M/N	25-152	[»]
3VGW	X-ray	1.60	A/B/C/D/E/F/G/H	25-147	[»]
3VHH	X-ray	2.26	A/B/C/D	25-147	[»]
3VHI	X-ray	1.76	A/B/C/D	26-147	[»]
3VHM	X-ray	2.00	A/B/C/D	25-147	[»]

ProteinModelPortal

P02701.

SMR

P02701. Positions 26-147.

ModBase

Search...

PTM databases

GlycoSuiteDB

P02701.

Proteomic databases

PaxDb

P02701.

PRIDE

P02701.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSGALT00000003855; ENSGALP00000003846; ENSGALG00000023622.
ENSGALT00000034429; ENSGALP00000033787; ENSGALG00000023622.

GeneID

396260.

KEGG

gga:396260.

Phylogenomic databases

eggNOG

NOG316013.

GeneTree

ENSGT00390000001847.

HOGENOM

HOG000034237.

HOVERGEN

HBG004323.

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-5341.

Gene expression databases

ArrayExpress

P02701.

Family and domain databases

Gene3D

2.40.128.30. 1 hit.

InterPro

IPR005469. Avidin.
IPR017889. Avidin-like_CS.
IPR005468. Avidin/str.
[Graphical view]

Pfam

PF01382. Avidin. 1 hit.
[Graphical view]

PRINTS

PR00709. AVIDIN.

SUPFAM

SSF50876. Avidin/str. 1 hit.

PROSITE

PS00577. AVIDIN_1. 1 hit.
PS51326. AVIDIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P02701.

NextBio

20816312.

Entry information

Entry name

AVID_CHICK

Accession

Primary (citable) accession number: P02701
Secondary accession number(s): Q91958, Q98SH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	September 11, 2007
Last modified:	April 3, 2013
This is version 119 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program