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P02701 (AVID_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein attributes

Sequence length152 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).

Subunit structure

Homotetramer.

Subcellular location

Secreted.

Tissue specificity

Synthesized in hen oviduct and concentrated in egg white (where it represent 0.05% of the total protein).

Post-translational modification

N-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)][Man(alpha1-?)Man(alpha1-6)] Man(beta1-4)GlcNAc(beta1-4)GlcNAc.

Sequence similarities

Belongs to the avidin/streptavidin family.

Contains 1 avidin-like domain.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityPolymorphism
   DomainSignal
   LigandBiotin
   PTMDisulfide bond
Glycoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Ref.5 Ref.6
Chain25 – 152128Avidin
PRO_0000002722

Regions

Domain26 – 149124Avidin-like

Sites

Binding site571Biotin

Amino acid modifications

Glycosylation411N-linked (GlcNAc...) Ref.5
CAR_000230
Disulfide bond28 ↔ 107

Natural variations

Natural variant581I → T in about 50% of the molecules. Ref.4 Ref.6

Experimental info

Sequence conflict221G → S in AAB59733. Ref.3
Sequence conflict351T → D in CAC34569. Ref.4
Sequence conflict501R → K in CAC34569. Ref.4
Sequence conflict771Q → E AA sequence Ref.5
Sequence conflict771Q → E AA sequence Ref.6

Secondary structure

........................... 152
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P02701 [UniParc].

Last modified September 11, 2007. Version 3.
Checksum: 7795A458481AEC09

FASTA15216,768
        10         20         30         40         50         60 
MVHATSPLLL LLLLSLALVA PGLSARKCSL TGKWTNDLGS NMTIGAVNSR GEFTGTYITA 

        70         80         90        100        110        120 
VTATSNEIKE SPLHGTQNTI NKRTQPTFGF TVNWKFSEST TVFTGQCFID RNGKEVLKTM 

       130        140        150 
WLLRSSVNDI GDDWKATRVG INIFTRLRTQ KE 

« Hide

References

[1]"Molecular cloning of the chicken avidin cDNA."
Gope M.L., Keinaenen R.A., Kristo P.A., Conneely O.M., Beatie W.G., Zarucki-Schulz T., O'Malley B.W., Kulomaa M.S.
Nucleic Acids Res. 15:3595-3606(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning and expression of avidin in Escherichia coli."
Chandra G., Gray J.G.
Methods Enzymol. 184:70-79(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Cloning and sequencing of the chicken egg-white avidin-encoding gene and its relationship with the avidin-related genes Avr1-Avr5."
Wallen M.J., Laukkanen M.O., Kulomaa M.S.
Gene 161:205-209(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: White leghorn.
Tissue: Oviduct.
[4]"Characterization and chromosomal localization of the chicken avidin gene family."
Ahlroth M.K., Kola E.H., Ewald D., Masabanda J., Sazanov A., Fries R., Kulomaa M.S.
Anim. Genet. 31:367-375(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-58.
[5]"Egg white avidin. 3. Sequence of the 78-residue middle cyanogen bromide peptide. Complete amino acid sequence of the protein subunit."
Delange R.J., Huang T.-S.
J. Biol. Chem. 246:698-709(1971) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-152, GLYCOSYLATION AT ASN-41.
[6]"Egg white avidin. II. Isolation, composition, and amino acid sequences of the tryptic peptides."
Huang T.-S., DeLange R.J.
J. Biol. Chem. 246:686-697(1971) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-152, VARIANT THR-58.
[7]"Studies on the biotin-binding sites of avidin and streptavidin. Tyrosine residues are involved in the binding site."
Gitlin G., Bayer E.A., Wilchek M.
Biochem. J. 269:527-530(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: IMPORTANCE OF TYR IN BIOTIN-BINDING.
[8]"Studies on the biotin-binding site of avidin. Minimized fragments that bind biotin."
Hiller Y., Bayer E.A., Wilchek M.
Biochem. J. 278:573-585(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOTIN-BINDING STUDIES.
[9]"Compositional and structural heterogeneity of avidin glycopeptides."
Bruch R.C., White H.B. III
Biochemistry 21:5334-5341(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE OF CARBOHYDRATE.
[10]"Three-dimensional structures of avidin and the avidin-biotin complex."
Livnah O., Bayer E.A., Wilchek M., Sussman J.L.
Proc. Natl. Acad. Sci. U.S.A. 90:5076-5080(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
[11]"Three-dimensional structure of the tetragonal crystal form of egg-white avidin in its functional complex with biotin at 2.7-A resolution."
Pugliese L., Coda A., Malcovati M., Bolognesi M.
J. Mol. Biol. 231:698-710(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
[12]"Biochemical characterization and crystal structure of a recombinant hen avidin and its acidic mutant expressed in Escherichia coli."
Nardone E., Rosano C., Santambrogio P., Curnis F., Corti A., Magni F., Siccardi A.G., Paganelli G., Losso R., Apreda B., Bolognesi M., Sidoli A., Arosio P.
Eur. J. Biochem. 256:453-460(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X05343 mRNA. Translation: CAA28954.1.
L27818 Genomic DNA. Translation: AAB59733.1.
AJ311647 Genomic DNA. Translation: CAC34569.1.
PIRVICH. A54975.
RefSeqNP_990651.1. NM_205320.1.
UniGeneGga.729.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AVDX-ray2.70A/B25-152[»]
1AVEX-ray2.80A/B25-152[»]
1IJ8X-ray2.00A/B25-152[»]
1LDOX-ray2.20A/B25-152[»]
1LDQX-ray2.70A/B25-152[»]
1LELX-ray2.90A/B25-152[»]
1NQNX-ray1.80A/B26-147[»]
1RAVX-ray2.20A/B26-152[»]
1VYOX-ray1.48A/B25-152[»]
2A5BX-ray2.49A/B25-148[»]
2A5CX-ray2.50A/B25-147[»]
2A8GX-ray1.99A/B27-152[»]
2AVIX-ray3.00A/B25-152[»]
2C4IX-ray1.95A27-147[»]
2CAMX-ray2.20A/B28-152[»]
2JGSX-ray1.90A/B/C/D24-152[»]
3FDCX-ray3.10A/B25-152[»]
3MM0X-ray2.70A/B/C/D/E/F/G/H/I/K/M/N25-152[»]
3VGWX-ray1.60A/B/C/D/E/F/G/H25-147[»]
3VHHX-ray2.26A/B/C/D25-147[»]
3VHIX-ray1.76A/B/C/D26-147[»]
3VHMX-ray2.00A/B/C/D25-147[»]
4I60X-ray2.50A25-152[»]
4JHQX-ray1.99A/B25-152[»]
ProteinModelPortalP02701.
SMRP02701. Positions 26-147.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60471N.

Chemistry

ChEMBLCHEMBL2189156.

PTM databases

UniCarbKBP02701.

Proteomic databases

PaxDbP02701.
PRIDEP02701.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSGALT00000003855; ENSGALP00000003846; ENSGALG00000023622.
GeneID396260.
KEGGgga:396260.

Organism-specific databases

CTD100127566.

Phylogenomic databases

eggNOGNOG316013.
GeneTreeENSGT00390000001847.
HOGENOMHOG000034237.
HOVERGENHBG004323.
OrthoDBEOG7PGDT6.
TreeFamTF336756.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-5341.

Family and domain databases

Gene3D2.40.128.30. 1 hit.
InterProIPR005469. Avidin.
IPR017889. Avidin-like_CS.
IPR005468. Avidin/str.
[Graphical view]
PfamPF01382. Avidin. 1 hit.
[Graphical view]
PRINTSPR00709. AVIDIN.
SUPFAMSSF50876. SSF50876. 1 hit.
PROSITEPS00577. AVIDIN_1. 1 hit.
PS51326. AVIDIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP02701.
NextBio20816312.

Entry information

Entry nameAVID_CHICK
AccessionPrimary (citable) accession number: P02701
Secondary accession number(s): Q91958, Q98SH4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: September 11, 2007
Last modified: February 19, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references