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Protein

Avidin

Gene

AVD

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei57 – 571Biotin

Keywords - Ligandi

Biotin

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-5341.

Names & Taxonomyi

Protein namesi
Recommended name:
Avidin
Gene namesi
Name:AVD
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539 Componenti: Chromosome Z

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24242 PublicationsAdd
BLAST
Chaini25 – 152128AvidinPRO_0000002722Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi28 ↔ 107
Glycosylationi41 – 411N-linked (GlcNAc...)1 PublicationCAR_000230

Post-translational modificationi

N-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)][Man(alpha1-?)Man(alpha1-6)] Man(beta1-4)GlcNAc(beta1-4)GlcNAc.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP02701.
PRIDEiP02701.

PTM databases

UniCarbKBiP02701.

Expressioni

Tissue specificityi

Synthesized in hen oviduct and concentrated in egg white (where it represent 0.05% of the total protein).

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

DIPiDIP-60471N.

Structurei

Secondary structure

1
152
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi32 – 365Combined sources
Beta strandi41 – 444Combined sources
Beta strandi51 – 588Combined sources
Beta strandi63 – 664Combined sources
Beta strandi71 – 777Combined sources
Helixi80 – 823Combined sources
Beta strandi87 – 937Combined sources
Beta strandi95 – 984Combined sources
Beta strandi100 – 10910Combined sources
Beta strandi111 – 1133Combined sources
Beta strandi115 – 12410Combined sources
Helixi130 – 1356Combined sources
Beta strandi137 – 14610Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AVDX-ray2.70A/B25-152[»]
1AVEX-ray2.80A/B25-152[»]
1IJ8X-ray2.00A/B25-152[»]
1LDOX-ray2.20A/B25-152[»]
1LDQX-ray2.70A/B25-152[»]
1LELX-ray2.90A/B25-152[»]
1NQNX-ray1.80A/B26-147[»]
1RAVX-ray2.20A/B26-152[»]
1VYOX-ray1.48A/B25-152[»]
2A5BX-ray2.49A/B25-148[»]
2A5CX-ray2.50A/B25-147[»]
2A8GX-ray1.99A/B27-152[»]
2AVIX-ray3.00A/B25-152[»]
2C4IX-ray1.95A22-80[»]
A99-147[»]
2CAMX-ray2.20A/B26-152[»]
2JGSX-ray1.90A/B/C/D69-152[»]
2MF6NMR-A/B/C/D25-61[»]
A/B/C/D85-152[»]
3FDCX-ray3.10A/B25-152[»]
3MM0X-ray2.70A/B/C/D/E/F/G/H/I/K/M/N25-61[»]
A/B/C/D/E/F/G/H/I/K/M/N85-152[»]
3VGWX-ray1.60A/B/C/D/E/F/G/H25-147[»]
3VHHX-ray2.26A/B/C/D25-147[»]
3VHIX-ray1.76A/B/C/D26-147[»]
3VHMX-ray2.00A/B/C/D25-147[»]
4I60X-ray2.50A25-152[»]
4JHQX-ray1.99A/B25-152[»]
SMRiP02701. Positions 26-147.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02701.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 149124Avidin-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the avidin/streptavidin family.Curated
Contains 1 avidin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG316013.
GeneTreeiENSGT00390000001847.
HOGENOMiHOG000034237.
HOVERGENiHBG004323.
InParanoidiP02701.
OMAiWKFSEST.
OrthoDBiEOG7PGDT6.
PhylomeDBiP02701.
TreeFamiTF336756.

Family and domain databases

Gene3Di2.40.128.30. 1 hit.
InterProiIPR005469. Avidin.
IPR017889. Avidin-like_CS.
IPR005468. Avidin/str.
[Graphical view]
PfamiPF01382. Avidin. 1 hit.
[Graphical view]
PRINTSiPR00709. AVIDIN.
SUPFAMiSSF50876. SSF50876. 1 hit.
PROSITEiPS00577. AVIDIN_1. 1 hit.
PS51326. AVIDIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02701-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVHATSPLLL LLLLSLALVA PGLSARKCSL TGKWTNDLGS NMTIGAVNSR
60 70 80 90 100
GEFTGTYITA VTATSNEIKE SPLHGTQNTI NKRTQPTFGF TVNWKFSEST
110 120 130 140 150
TVFTGQCFID RNGKEVLKTM WLLRSSVNDI GDDWKATRVG INIFTRLRTQ

KE
Length:152
Mass (Da):16,768
Last modified:September 11, 2007 - v3
Checksum:i7795A458481AEC09
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221G → S in AAB59733 (PubMed:7665080).Curated
Sequence conflicti35 – 351T → D in CAC34569 (PubMed:11167523).Curated
Sequence conflicti50 – 501R → K in CAC34569 (PubMed:11167523).Curated
Sequence conflicti77 – 771Q → E AA sequence (PubMed:5100763).Curated
Sequence conflicti77 – 771Q → E AA sequence (PubMed:5100762).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti58 – 581I → T in about 50% of the molecules. 2 Publications

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05343 mRNA. Translation: CAA28954.1.
L27818 Genomic DNA. Translation: AAB59733.1.
AJ311647 Genomic DNA. Translation: CAC34569.1.
PIRiA54975. VICH.
RefSeqiNP_990651.1. NM_205320.1.
UniGeneiGga.729.

Genome annotation databases

EnsembliENSGALT00000003855; ENSGALP00000003846; ENSGALG00000023622.
GeneIDi396260.
KEGGigga:396260.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05343 mRNA. Translation: CAA28954.1.
L27818 Genomic DNA. Translation: AAB59733.1.
AJ311647 Genomic DNA. Translation: CAC34569.1.
PIRiA54975. VICH.
RefSeqiNP_990651.1. NM_205320.1.
UniGeneiGga.729.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AVDX-ray2.70A/B25-152[»]
1AVEX-ray2.80A/B25-152[»]
1IJ8X-ray2.00A/B25-152[»]
1LDOX-ray2.20A/B25-152[»]
1LDQX-ray2.70A/B25-152[»]
1LELX-ray2.90A/B25-152[»]
1NQNX-ray1.80A/B26-147[»]
1RAVX-ray2.20A/B26-152[»]
1VYOX-ray1.48A/B25-152[»]
2A5BX-ray2.49A/B25-148[»]
2A5CX-ray2.50A/B25-147[»]
2A8GX-ray1.99A/B27-152[»]
2AVIX-ray3.00A/B25-152[»]
2C4IX-ray1.95A22-80[»]
A99-147[»]
2CAMX-ray2.20A/B26-152[»]
2JGSX-ray1.90A/B/C/D69-152[»]
2MF6NMR-A/B/C/D25-61[»]
A/B/C/D85-152[»]
3FDCX-ray3.10A/B25-152[»]
3MM0X-ray2.70A/B/C/D/E/F/G/H/I/K/M/N25-61[»]
A/B/C/D/E/F/G/H/I/K/M/N85-152[»]
3VGWX-ray1.60A/B/C/D/E/F/G/H25-147[»]
3VHHX-ray2.26A/B/C/D25-147[»]
3VHIX-ray1.76A/B/C/D26-147[»]
3VHMX-ray2.00A/B/C/D25-147[»]
4I60X-ray2.50A25-152[»]
4JHQX-ray1.99A/B25-152[»]
SMRiP02701. Positions 26-147.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60471N.

Chemistry

ChEMBLiCHEMBL2189156.

PTM databases

UniCarbKBiP02701.

Proteomic databases

PaxDbiP02701.
PRIDEiP02701.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000003855; ENSGALP00000003846; ENSGALG00000023622.
GeneIDi396260.
KEGGigga:396260.

Organism-specific databases

CTDi567678.

Phylogenomic databases

eggNOGiNOG316013.
GeneTreeiENSGT00390000001847.
HOGENOMiHOG000034237.
HOVERGENiHBG004323.
InParanoidiP02701.
OMAiWKFSEST.
OrthoDBiEOG7PGDT6.
PhylomeDBiP02701.
TreeFamiTF336756.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-5341.

Miscellaneous databases

EvolutionaryTraceiP02701.
NextBioi20816312.

Family and domain databases

Gene3Di2.40.128.30. 1 hit.
InterProiIPR005469. Avidin.
IPR017889. Avidin-like_CS.
IPR005468. Avidin/str.
[Graphical view]
PfamiPF01382. Avidin. 1 hit.
[Graphical view]
PRINTSiPR00709. AVIDIN.
SUPFAMiSSF50876. SSF50876. 1 hit.
PROSITEiPS00577. AVIDIN_1. 1 hit.
PS51326. AVIDIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and expression of avidin in Escherichia coli."
    Chandra G., Gray J.G.
    Methods Enzymol. 184:70-79(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Cloning and sequencing of the chicken egg-white avidin-encoding gene and its relationship with the avidin-related genes Avr1-Avr5."
    Wallen M.J., Laukkanen M.O., Kulomaa M.S.
    Gene 161:205-209(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: White leghorn.
    Tissue: Oviduct.
  4. "Characterization and chromosomal localization of the chicken avidin gene family."
    Ahlroth M.K., Kola E.H., Ewald D., Masabanda J., Sazanov A., Fries R., Kulomaa M.S.
    Anim. Genet. 31:367-375(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-58.
  5. "Egg white avidin. 3. Sequence of the 78-residue middle cyanogen bromide peptide. Complete amino acid sequence of the protein subunit."
    Delange R.J., Huang T.-S.
    J. Biol. Chem. 246:698-709(1970) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-152, GLYCOSYLATION AT ASN-41.
  6. "Egg white avidin. II. Isolation, composition, and amino acid sequences of the tryptic peptides."
    Huang T.-S., DeLange R.J.
    J. Biol. Chem. 246:686-697(1970) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-152, VARIANT THR-58.
  7. "Studies on the biotin-binding sites of avidin and streptavidin. Tyrosine residues are involved in the binding site."
    Gitlin G., Bayer E.A., Wilchek M.
    Biochem. J. 269:527-530(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: IMPORTANCE OF TYR IN BIOTIN-BINDING.
  8. "Studies on the biotin-binding site of avidin. Minimized fragments that bind biotin."
    Hiller Y., Bayer E.A., Wilchek M.
    Biochem. J. 278:573-585(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOTIN-BINDING STUDIES.
  9. "Compositional and structural heterogeneity of avidin glycopeptides."
    Bruch R.C., White H.B. III
    Biochemistry 21:5334-5341(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE OF CARBOHYDRATE.
  10. "Three-dimensional structures of avidin and the avidin-biotin complex."
    Livnah O., Bayer E.A., Wilchek M., Sussman J.L.
    Proc. Natl. Acad. Sci. U.S.A. 90:5076-5080(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
  11. "Three-dimensional structure of the tetragonal crystal form of egg-white avidin in its functional complex with biotin at 2.7-A resolution."
    Pugliese L., Coda A., Malcovati M., Bolognesi M.
    J. Mol. Biol. 231:698-710(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
  12. "Biochemical characterization and crystal structure of a recombinant hen avidin and its acidic mutant expressed in Escherichia coli."
    Nardone E., Rosano C., Santambrogio P., Curnis F., Corti A., Magni F., Siccardi A.G., Paganelli G., Losso R., Apreda B., Bolognesi M., Sidoli A., Arosio P.
    Eur. J. Biochem. 256:453-460(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).

Entry informationi

Entry nameiAVID_CHICK
AccessioniPrimary (citable) accession number: P02701
Secondary accession number(s): Q91958, Q98SH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: September 11, 2007
Last modified: April 1, 2015
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.