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P02701

- AVID_CHICK

UniProt

P02701 - AVID_CHICK

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Protein

Avidin

Gene

AVD

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei57 – 571Biotin

Keywords - Ligandi

Biotin

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-5341.

Names & Taxonomyi

Protein namesi
Recommended name:
Avidin
Gene namesi
Name:AVD
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Chromosome Z

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24242 PublicationsAdd
BLAST
Chaini25 – 152128AvidinPRO_0000002722Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi28 ↔ 107
Glycosylationi41 – 411N-linked (GlcNAc...)1 PublicationCAR_000230

Post-translational modificationi

N-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)][Man(alpha1-?)Man(alpha1-6)] Man(beta1-4)GlcNAc(beta1-4)GlcNAc.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP02701.
PRIDEiP02701.

PTM databases

UniCarbKBiP02701.

Expressioni

Tissue specificityi

Synthesized in hen oviduct and concentrated in egg white (where it represent 0.05% of the total protein).

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

DIPiDIP-60471N.

Structurei

Secondary structure

1
152
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi32 – 365
Beta strandi41 – 444
Beta strandi51 – 588
Beta strandi63 – 664
Beta strandi71 – 777
Helixi80 – 823
Beta strandi87 – 937
Beta strandi95 – 984
Beta strandi100 – 10910
Beta strandi111 – 1133
Beta strandi115 – 12410
Helixi130 – 1356
Beta strandi137 – 14610

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AVDX-ray2.70A/B25-152[»]
1AVEX-ray2.80A/B25-152[»]
1IJ8X-ray2.00A/B25-152[»]
1LDOX-ray2.20A/B25-152[»]
1LDQX-ray2.70A/B25-152[»]
1LELX-ray2.90A/B25-152[»]
1NQNX-ray1.80A/B26-147[»]
1RAVX-ray2.20A/B26-152[»]
1VYOX-ray1.48A/B25-152[»]
2A5BX-ray2.49A/B25-148[»]
2A5CX-ray2.50A/B25-147[»]
2A8GX-ray1.99A/B27-152[»]
2AVIX-ray3.00A/B25-152[»]
2C4IX-ray1.95A22-80[»]
A99-147[»]
2CAMX-ray2.20A/B26-152[»]
2JGSX-ray1.90A/B/C/D69-152[»]
2MF6NMR-A/B/C/D25-61[»]
A/B/C/D85-152[»]
3FDCX-ray3.10A/B25-152[»]
3MM0X-ray2.70A/B/C/D/E/F/G/H/I/K/M/N25-61[»]
A/B/C/D/E/F/G/H/I/K/M/N85-152[»]
3VGWX-ray1.60A/B/C/D/E/F/G/H25-147[»]
3VHHX-ray2.26A/B/C/D25-147[»]
3VHIX-ray1.76A/B/C/D26-147[»]
3VHMX-ray2.00A/B/C/D25-147[»]
4I60X-ray2.50A25-152[»]
4JHQX-ray1.99A/B25-152[»]
ProteinModelPortaliP02701.
SMRiP02701. Positions 26-147.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02701.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 149124Avidin-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the avidin/streptavidin family.Curated
Contains 1 avidin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG316013.
GeneTreeiENSGT00390000001847.
HOGENOMiHOG000034237.
HOVERGENiHBG004323.
InParanoidiP02701.
OrthoDBiEOG7PGDT6.
PhylomeDBiP02701.
TreeFamiTF336756.

Family and domain databases

Gene3Di2.40.128.30. 1 hit.
InterProiIPR005469. Avidin.
IPR017889. Avidin-like_CS.
IPR005468. Avidin/str.
[Graphical view]
PfamiPF01382. Avidin. 1 hit.
[Graphical view]
PRINTSiPR00709. AVIDIN.
SUPFAMiSSF50876. SSF50876. 1 hit.
PROSITEiPS00577. AVIDIN_1. 1 hit.
PS51326. AVIDIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02701-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVHATSPLLL LLLLSLALVA PGLSARKCSL TGKWTNDLGS NMTIGAVNSR
60 70 80 90 100
GEFTGTYITA VTATSNEIKE SPLHGTQNTI NKRTQPTFGF TVNWKFSEST
110 120 130 140 150
TVFTGQCFID RNGKEVLKTM WLLRSSVNDI GDDWKATRVG INIFTRLRTQ

KE
Length:152
Mass (Da):16,768
Last modified:September 11, 2007 - v3
Checksum:i7795A458481AEC09
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221G → S in AAB59733. (PubMed:7665080)Curated
Sequence conflicti35 – 351T → D in CAC34569. (PubMed:11167523)Curated
Sequence conflicti50 – 501R → K in CAC34569. (PubMed:11167523)Curated
Sequence conflicti77 – 771Q → E AA sequence (PubMed:5100763)Curated
Sequence conflicti77 – 771Q → E AA sequence (PubMed:5100762)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti58 – 581I → T in about 50% of the molecules. 2 Publications

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X05343 mRNA. Translation: CAA28954.1.
L27818 Genomic DNA. Translation: AAB59733.1.
AJ311647 Genomic DNA. Translation: CAC34569.1.
PIRiA54975. VICH.
RefSeqiNP_990651.1. NM_205320.1.
UniGeneiGga.729.

Genome annotation databases

EnsembliENSGALT00000003855; ENSGALP00000003846; ENSGALG00000023622.
GeneIDi396260.
KEGGigga:396260.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X05343 mRNA. Translation: CAA28954.1 .
L27818 Genomic DNA. Translation: AAB59733.1 .
AJ311647 Genomic DNA. Translation: CAC34569.1 .
PIRi A54975. VICH.
RefSeqi NP_990651.1. NM_205320.1.
UniGenei Gga.729.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1AVD X-ray 2.70 A/B 25-152 [» ]
1AVE X-ray 2.80 A/B 25-152 [» ]
1IJ8 X-ray 2.00 A/B 25-152 [» ]
1LDO X-ray 2.20 A/B 25-152 [» ]
1LDQ X-ray 2.70 A/B 25-152 [» ]
1LEL X-ray 2.90 A/B 25-152 [» ]
1NQN X-ray 1.80 A/B 26-147 [» ]
1RAV X-ray 2.20 A/B 26-152 [» ]
1VYO X-ray 1.48 A/B 25-152 [» ]
2A5B X-ray 2.49 A/B 25-148 [» ]
2A5C X-ray 2.50 A/B 25-147 [» ]
2A8G X-ray 1.99 A/B 27-152 [» ]
2AVI X-ray 3.00 A/B 25-152 [» ]
2C4I X-ray 1.95 A 22-80 [» ]
A 99-147 [» ]
2CAM X-ray 2.20 A/B 26-152 [» ]
2JGS X-ray 1.90 A/B/C/D 69-152 [» ]
2MF6 NMR - A/B/C/D 25-61 [» ]
A/B/C/D 85-152 [» ]
3FDC X-ray 3.10 A/B 25-152 [» ]
3MM0 X-ray 2.70 A/B/C/D/E/F/G/H/I/K/M/N 25-61 [» ]
A/B/C/D/E/F/G/H/I/K/M/N 85-152 [» ]
3VGW X-ray 1.60 A/B/C/D/E/F/G/H 25-147 [» ]
3VHH X-ray 2.26 A/B/C/D 25-147 [» ]
3VHI X-ray 1.76 A/B/C/D 26-147 [» ]
3VHM X-ray 2.00 A/B/C/D 25-147 [» ]
4I60 X-ray 2.50 A 25-152 [» ]
4JHQ X-ray 1.99 A/B 25-152 [» ]
ProteinModelPortali P02701.
SMRi P02701. Positions 26-147.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60471N.

Chemistry

ChEMBLi CHEMBL2189156.

PTM databases

UniCarbKBi P02701.

Proteomic databases

PaxDbi P02701.
PRIDEi P02701.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSGALT00000003855 ; ENSGALP00000003846 ; ENSGALG00000023622 .
GeneIDi 396260.
KEGGi gga:396260.

Organism-specific databases

CTDi 567678.

Phylogenomic databases

eggNOGi NOG316013.
GeneTreei ENSGT00390000001847.
HOGENOMi HOG000034237.
HOVERGENi HBG004323.
InParanoidi P02701.
OrthoDBi EOG7PGDT6.
PhylomeDBi P02701.
TreeFami TF336756.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-5341.

Miscellaneous databases

EvolutionaryTracei P02701.
NextBioi 20816312.

Family and domain databases

Gene3Di 2.40.128.30. 1 hit.
InterProi IPR005469. Avidin.
IPR017889. Avidin-like_CS.
IPR005468. Avidin/str.
[Graphical view ]
Pfami PF01382. Avidin. 1 hit.
[Graphical view ]
PRINTSi PR00709. AVIDIN.
SUPFAMi SSF50876. SSF50876. 1 hit.
PROSITEi PS00577. AVIDIN_1. 1 hit.
PS51326. AVIDIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and expression of avidin in Escherichia coli."
    Chandra G., Gray J.G.
    Methods Enzymol. 184:70-79(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Cloning and sequencing of the chicken egg-white avidin-encoding gene and its relationship with the avidin-related genes Avr1-Avr5."
    Wallen M.J., Laukkanen M.O., Kulomaa M.S.
    Gene 161:205-209(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: White leghorn.
    Tissue: Oviduct.
  4. "Characterization and chromosomal localization of the chicken avidin gene family."
    Ahlroth M.K., Kola E.H., Ewald D., Masabanda J., Sazanov A., Fries R., Kulomaa M.S.
    Anim. Genet. 31:367-375(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-58.
  5. "Egg white avidin. 3. Sequence of the 78-residue middle cyanogen bromide peptide. Complete amino acid sequence of the protein subunit."
    Delange R.J., Huang T.-S.
    J. Biol. Chem. 246:698-709(1971) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-152, GLYCOSYLATION AT ASN-41.
  6. "Egg white avidin. II. Isolation, composition, and amino acid sequences of the tryptic peptides."
    Huang T.-S., DeLange R.J.
    J. Biol. Chem. 246:686-697(1971) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-152, VARIANT THR-58.
  7. "Studies on the biotin-binding sites of avidin and streptavidin. Tyrosine residues are involved in the binding site."
    Gitlin G., Bayer E.A., Wilchek M.
    Biochem. J. 269:527-530(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: IMPORTANCE OF TYR IN BIOTIN-BINDING.
  8. "Studies on the biotin-binding site of avidin. Minimized fragments that bind biotin."
    Hiller Y., Bayer E.A., Wilchek M.
    Biochem. J. 278:573-585(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOTIN-BINDING STUDIES.
  9. "Compositional and structural heterogeneity of avidin glycopeptides."
    Bruch R.C., White H.B. III
    Biochemistry 21:5334-5341(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE OF CARBOHYDRATE.
  10. "Three-dimensional structures of avidin and the avidin-biotin complex."
    Livnah O., Bayer E.A., Wilchek M., Sussman J.L.
    Proc. Natl. Acad. Sci. U.S.A. 90:5076-5080(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
  11. "Three-dimensional structure of the tetragonal crystal form of egg-white avidin in its functional complex with biotin at 2.7-A resolution."
    Pugliese L., Coda A., Malcovati M., Bolognesi M.
    J. Mol. Biol. 231:698-710(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
  12. "Biochemical characterization and crystal structure of a recombinant hen avidin and its acidic mutant expressed in Escherichia coli."
    Nardone E., Rosano C., Santambrogio P., Curnis F., Corti A., Magni F., Siccardi A.G., Paganelli G., Losso R., Apreda B., Bolognesi M., Sidoli A., Arosio P.
    Eur. J. Biochem. 256:453-460(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).

Entry informationi

Entry nameiAVID_CHICK
AccessioniPrimary (citable) accession number: P02701
Secondary accession number(s): Q91958, Q98SH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: September 11, 2007
Last modified: October 29, 2014
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3