Reviewed, UniProtKB/Swiss-Prot P02700 (OPSD_SHEEP)

Names and origin

Protein names

Recommended name:
    Rhodopsin

Gene names

Name:

RHO

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Rod shaped photoreceptor cells which mediates vision in dim light.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
biophysicochemical properties	Absorption: Abs(max)=495 nm

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Membrane
Domain	Transmembrane
Ligand	Chromophore
Molecular function	G-protein coupled receptor Photoreceptor protein Receptor Retinal protein Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW phototransduction Inferred from electronic annotation. Source: UniProtKB-KW protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW photoreceptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	Rhodopsin		PRO_0000197719
Regions
	Topological domain	1 – 36	36	Extracellular
	Transmembrane	37 – 61	25	1 Potential
	Topological domain	62 – 73	12	Cytoplasmic
	Transmembrane	74 – 98	25	2 Potential
	Topological domain	99 – 113	15	Extracellular
	Transmembrane	114 – 133	20	3 Potential
	Topological domain	134 – 152	19	Cytoplasmic
	Transmembrane	153 – 176	24	4 Potential
	Topological domain	177 – 202	26	Extracellular
	Transmembrane	203 – 230	28	5 Potential
	Topological domain	231 – 252	22	Cytoplasmic
	Transmembrane	253 – 276	24	6 Potential
	Topological domain	277 – 284	8	Extracellular
	Transmembrane	285 – 309	25	7 Potential
	Topological domain	310 – 348	39	Cytoplasmic
Amino acid modifications
	Modified residue	296	1	N6-(retinylidene)lysine
	Modified residue	334	1	Phosphoserine; by RK Ref.5
	Modified residue	335	1	Phosphothreonine; by RK Ref.5
	Modified residue	336	1	Phosphothreonine; by RK Ref.5
	Modified residue	338	1	Phosphoserine; by RK Ref.5
	Modified residue	343	1	Phosphoserine; by RK Ref.5
	Lipidation	322	1	S-palmitoyl cysteine By similarity
	Lipidation	323	1	S-palmitoyl cysteine By similarity
	Glycosylation	2	1	N-linked (GlcNAc...) By similarity
	Glycosylation	15	1	N-linked (GlcNAc...) By similarity
	Disulfide bond	110 ↔ 187		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P02700-1 [UniParc]. Last modified February 1, 1991. Version 2. Checksum: AAFD6F0D6A8BAEE5	FASTA	348	38,892
	10 20 30 40 50 60 MNGTEGPNFY VPFSNKTGVV RSPFEAPQYY LAEPWQFSML AAYMFLLIVL GFPINFLTLY 70 80 90 100 110 120 VTVQHKKLRT PLNYILLNLA VADLFMVFGG FTTTLYTSLH GYFVFGPTGC NLEGFFATLG 130 140 150 160 170 180 GEIALWSLVV LAIERYVVVC KPMSNFRFGE NHAIMGVAFT WVMALACAAP PLVGWSRYIP 190 200 210 220 230 240 QGMQCSCGAL YFTLKPEINN ESFVIYMFVV HFSIPLIVIF FCYGQLVFTV KEAAAQQQES 250 260 270 280 290 300 ATTQKAEKEV TRMVIIMVIA FLICWLPYAG VAFYIFTHQG SDFGPIFMTI PAFFAKSSSV 310 320 330 340 YNPVIYIMMN KQFRNCMLTT LCCGKNPLGD DEASTTVSKT ETSQVAPA

References

[1]	"A structural model for ovine rhodopsin." Pappin D.J.C., Elipoulos E., Brett M., Findlay J.B.C. Int. J. Biol. Macromol. 6:73-76(1984) Cited for: PROTEIN SEQUENCE.
[2]	"Isolation and characterization of the CNBr peptides from the proteolytically derived N-terminal fragment of ovine opsin." Brett M., Findlay J.B.C. Biochem. J. 211:661-670(1983) [PubMed: 6224479] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-111 AND 144-239.
[3]	"Primary structure of C-terminal functional sites in ovine rhodopsin." Findlay J.B.C., Brett M., Pappin D.J.C. Nature 293:314-316(1981) [PubMed: 7278988] [Abstract] Cited for: PROTEIN SEQUENCE OF 240-348.
[4]	"Sequence variability in the retinal-attachment domain of mammalian rhodopsins." Pappin D.J.C., Findlay J.B.C. Biochem. J. 217:605-613(1984) [PubMed: 6370231] [Abstract] Cited for: RETINAL-BINDING SITE.
[5]	"Phosphorylation of ovine rhodopsin. Identification of the phosphorylated sites." Thompson P., Findlay J.B.C. Biochem. J. 220:773-780(1984) [PubMed: 6466303] [Abstract] Cited for: PHOSPHORYLATION AT SER-334; THR-335; THR-336; SER-338 AND SER-343.

Cross-references

Sequence databases
PIR	OOSH. A30407.
3D structure databases
HSSP	HSSP built from PDB template 1EDS based on UniProtKB P02699.
SMR	P02700. Positions 1-348.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Phylogenomic databases
HOVERGEN	P02700.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR001760. Opsin. IPR000732. Rhodopsin. IPR019477. Rhodopsin_N. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. PF10413. Rhodopsin_N. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00238. OPSIN. PR00579. RHODOPSIN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. PS00238. OPSIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OPSD_SHEEP

Accession

Primary (citable) accession number: P02700

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	February 1, 1991
Last modified:	June 16, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)