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Protein

Myelin basic protein

Gene

Mbp

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane (By similarity).By similarity

GO - Molecular functioni

  • structural constituent of myelin sheath Source: RGD

GO - Biological processi

  • aging Source: RGD
  • central nervous system development Source: GO_Central
  • myelination Source: RGD
  • negative regulation of axonogenesis Source: RGD
  • response to fatty acid Source: RGD
  • response to mercury ion Source: RGD
  • response to progesterone Source: RGD
  • response to tumor necrosis factor Source: RGD
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Myelin basic protein
Short name:
MBP
Gene namesi
Name:Mbp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 18

Organism-specific databases

RGDi3054. Mbp.

Subcellular locationi

GO - Cellular componenti

  • cell periphery Source: GO_Central
  • compact myelin Source: RGD
  • internode region of axon Source: GO_Central
  • myelin sheath Source: UniProtKB
  • neuronal cell body Source: GO_Central
  • plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Keywords - Diseasei

Autoimmune encephalomyelitis

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001589952 – 195Myelin basic proteinAdd BLAST194

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei8PhosphoserineBy similarity1
Modified residuei13PhosphoserineBy similarity1
Modified residuei15PhosphotyrosineCombined sources1
Modified residuei18PhosphothreonineCombined sources1
Modified residuei20PhosphoserineCombined sources1
Modified residuei21PhosphothreonineCombined sources1
Modified residuei26CitrullineBy similarity1
Modified residuei32CitrullineBy similarity1
Modified residuei36PhosphothreonineCombined sources1
Modified residuei41PhosphoserineCombined sources1
Modified residuei44Omega-N-methylarginineBy similarity1
Modified residuei50Omega-N-methylarginineBy similarity1
Modified residuei57PhosphoserineBy similarity1
Modified residuei92PhosphothreonineBy similarity1
Modified residuei94PhosphotyrosineBy similarity1
Modified residuei101PhosphoserineBy similarity1
Modified residuei104PhosphothreonineBy similarity1
Modified residuei119Phosphothreonine1 Publication1
Modified residuei122PhosphothreonineBy similarity1
Modified residuei127Deamidated glutamineBy similarity1
Modified residuei131Omega-N-methylarginine; alternate1 Publication1
Modified residuei131Symmetric dimethylarginine; alternate1 Publication1
Modified residuei139PhosphoserineBy similarity1
Modified residuei146N6-acetyllysineBy similarity1
Modified residuei154CitrullineBy similarity1
Modified residuei172Deamidated glutamineBy similarity1
Modified residuei184CitrullineBy similarity1
Modified residuei186PhosphoserineBy similarity1
Modified residuei190Phosphoserine; by UHMK1By similarity1
Modified residuei195CitrullineBy similarity1

Post-translational modificationi

As in other animals, several charge isomers may be produced as a result of optional post-translatonial modifications, such as phosphorylation of serine or threonine residues, deamidation of glutamine or asparagine residues, citrullination and methylation of arginine residues.2 Publications
Arg-131 was found to be 44% monomethylated and 11% symmetrically dimethylated.
Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1.By similarity

Keywords - PTMi

Acetylation, Citrullination, Methylation, Phosphoprotein

Proteomic databases

PaxDbiP02688.
PRIDEiP02688.

PTM databases

iPTMnetiP02688.
PhosphoSitePlusiP02688.
SwissPalmiP02688.

Miscellaneous databases

PMAP-CutDBP02688.

Expressioni

Tissue specificityi

Found in both the central and the peripheral nervous system.

Gene expression databases

BgeeiENSRNOG00000016516.
ExpressionAtlasiP02688. baseline and differential.
GenevisibleiP02688. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Kcnma1Q629764EBI-1638296,EBI-1638146

Protein-protein interaction databases

BioGridi246698. 6 interactors.
IntActiP02688. 2 interactors.
MINTiMINT-248798.
STRINGi10116.ENSRNOP00000022303.

Family & Domainsi

Sequence similaritiesi

Belongs to the myelin basic protein family.Curated

Phylogenomic databases

eggNOGiENOG410IIUJ. Eukaryota.
ENOG4111PMJ. LUCA.
GeneTreeiENSGT00390000014772.
HOGENOMiHOG000293395.
HOVERGENiHBG008347.
InParanoidiP02688.
KOiK17269.
OMAiKKRTTHY.

Family and domain databases

InterProiIPR000548. Myelin_BP.
[Graphical view]
PANTHERiPTHR11429. PTHR11429. 2 hits.
PfamiPF01669. Myelin_MBP. 1 hit.
[Graphical view]
PRINTSiPR00212. MYELINMBP.
PROSITEiPS00569. MYELIN_MBP. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: P02688-1) [UniParc]FASTAAdd to basket
Also known as: 21.5 kDa

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASQKRPSQR HGSKYLATAS TMDHARHGFL PRHRDTGILD SIGRFFSGDR
60 70 80 90 100
GAPKRGSGKV PWLKQSRSPL PSHARSRPGL CHMYKDSHTR TTHYGSLPQK
110 120 130 140 150
SQRTQDENPV VHFFKNIVTP RTPPPSQGKG RGLSLSRFSW GAEGQKPGFG
160 170 180 190
YGGRASDYKS AHKGFKGAYD AQGTLSKIFK LGGRDSRSGS PMARR
Length:195
Mass (Da):21,502
Last modified:January 23, 2007 - v3
Checksum:iF1A43933EC9D4CFF
GO
Isoform 2 (identifier: P02688-2) [UniParc]FASTAAdd to basket
Also known as: 18.5 kDa, MBP L

The sequence of this isoform differs from the canonical sequence as follows:
     60-85: Missing.

Show »
Length:169
Mass (Da):18,488
Checksum:iB2D67AB198FC7D11
GO
Isoform 3 (identifier: P02688-3) [UniParc]FASTAAdd to basket
Also known as: 17 kDa

The sequence of this isoform differs from the canonical sequence as follows:
     141-181: Missing.

Show »
Length:154
Mass (Da):17,225
Checksum:iEFB722E97213E17A
GO
Isoform 4 (identifier: P02688-4) [UniParc]FASTAAdd to basket
Also known as: 14 kDa, MBP S, smaller myelin basic protein

The sequence of this isoform differs from the canonical sequence as follows:
     60-85: Missing.
     141-181: Missing.

Show »
Length:128
Mass (Da):14,211
Checksum:i2DAF033C19CF111C
GO
Isoform 5 (identifier: P02688-5) [UniParc]FASTAAdd to basket
Also known as: 17 kDa

The sequence of this isoform differs from the canonical sequence as follows:
     60-85: Missing.
     130-140: Missing.

Show »
Length:158
Mass (Da):17,240
Checksum:i3256580242ECC3E1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti47 – 48SG → GS AA sequence (PubMed:4122324).Curated2
Sequence conflicti192M → I in CAA10804 (Ref. 1) Curated1
Sequence conflicti192M → I in CAA10805 (Ref. 1) Curated1
Sequence conflicti192M → I in CAA10806 (Ref. 1) Curated1
Sequence conflicti192M → I in CAA10807 (Ref. 1) Curated1
Sequence conflicti192M → I (PubMed:6194889).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00332160 – 85Missing in isoform 2, isoform 4 and isoform 5. 5 PublicationsAdd BLAST26
Alternative sequenceiVSP_025711130 – 140Missing in isoform 5. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_003322141 – 181Missing in isoform 3 and isoform 4. 4 PublicationsAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132895 mRNA. Translation: CAA10804.1.
AJ132896 mRNA. Translation: CAA10805.1.
AJ132897 mRNA. Translation: CAA10806.1.
AJ132898 mRNA. Translation: CAA10807.1.
M25889 mRNA. Translation: AAA41575.1.
K00512 mRNA. No translation available.
AF439750 mRNA. Translation: AAL84189.1.
BC094522 mRNA. Translation: AAH94522.1.
X72392 mRNA. No translation available.
PIRiB24351. MBRTS.
RefSeqiNP_001020462.1. NM_001025291.1. [P02688-1]
NP_001020463.1. NM_001025292.1. [P02688-2]
NP_001020464.1. NM_001025293.1. [P02688-3]
NP_001020465.1. NM_001025294.1. [P02688-5]
NP_058722.1. NM_017026.2. [P02688-4]
UniGeneiRn.203146.
Rn.63285.

Genome annotation databases

EnsembliENSRNOT00000022280; ENSRNOP00000022280; ENSRNOG00000016516. [P02688-3]
ENSRNOT00000022303; ENSRNOP00000022303; ENSRNOG00000016516. [P02688-1]
ENSRNOT00000058295; ENSRNOP00000055097; ENSRNOG00000016516. [P02688-2]
ENSRNOT00000058296; ENSRNOP00000055098; ENSRNOG00000016516. [P02688-5]
GeneIDi24547.
KEGGirno:24547.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132895 mRNA. Translation: CAA10804.1.
AJ132896 mRNA. Translation: CAA10805.1.
AJ132897 mRNA. Translation: CAA10806.1.
AJ132898 mRNA. Translation: CAA10807.1.
M25889 mRNA. Translation: AAA41575.1.
K00512 mRNA. No translation available.
AF439750 mRNA. Translation: AAL84189.1.
BC094522 mRNA. Translation: AAH94522.1.
X72392 mRNA. No translation available.
PIRiB24351. MBRTS.
RefSeqiNP_001020462.1. NM_001025291.1. [P02688-1]
NP_001020463.1. NM_001025292.1. [P02688-2]
NP_001020464.1. NM_001025293.1. [P02688-3]
NP_001020465.1. NM_001025294.1. [P02688-5]
NP_058722.1. NM_017026.2. [P02688-4]
UniGeneiRn.203146.
Rn.63285.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246698. 6 interactors.
IntActiP02688. 2 interactors.
MINTiMINT-248798.
STRINGi10116.ENSRNOP00000022303.

PTM databases

iPTMnetiP02688.
PhosphoSitePlusiP02688.
SwissPalmiP02688.

Proteomic databases

PaxDbiP02688.
PRIDEiP02688.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022280; ENSRNOP00000022280; ENSRNOG00000016516. [P02688-3]
ENSRNOT00000022303; ENSRNOP00000022303; ENSRNOG00000016516. [P02688-1]
ENSRNOT00000058295; ENSRNOP00000055097; ENSRNOG00000016516. [P02688-2]
ENSRNOT00000058296; ENSRNOP00000055098; ENSRNOG00000016516. [P02688-5]
GeneIDi24547.
KEGGirno:24547.

Organism-specific databases

CTDi4155.
RGDi3054. Mbp.

Phylogenomic databases

eggNOGiENOG410IIUJ. Eukaryota.
ENOG4111PMJ. LUCA.
GeneTreeiENSGT00390000014772.
HOGENOMiHOG000293395.
HOVERGENiHBG008347.
InParanoidiP02688.
KOiK17269.
OMAiKKRTTHY.

Miscellaneous databases

PMAP-CutDBP02688.
PROiP02688.

Gene expression databases

BgeeiENSRNOG00000016516.
ExpressionAtlasiP02688. baseline and differential.
GenevisibleiP02688. RN.

Family and domain databases

InterProiIPR000548. Myelin_BP.
[Graphical view]
PANTHERiPTHR11429. PTHR11429. 2 hits.
PfamiPF01669. Myelin_MBP. 1 hit.
[Graphical view]
PRINTSiPR00212. MYELINMBP.
PROSITEiPS00569. MYELIN_MBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMBP_RAT
AccessioniPrimary (citable) accession number: P02688
Secondary accession number(s): Q505J1
, Q8R4K6, Q9Z1J4, Q9Z1J5, Q9Z1J6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 151 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.