Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Myelin basic protein

Gene

Mbp

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane (By similarity).By similarity

GO - Molecular functioni

  • structural constituent of myelin sheath Source: RGD

GO - Biological processi

  • aging Source: RGD
  • central nervous system development Source: GO_Central
  • myelination Source: RGD
  • negative regulation of axonogenesis Source: RGD
  • response to fatty acid Source: RGD
  • response to mercury ion Source: RGD
  • response to progesterone Source: RGD
  • response to tumor necrosis factor Source: RGD
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Myelin basic protein
Short name:
MBP
Gene namesi
Name:Mbp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 18

Organism-specific databases

RGDi3054. Mbp.

Subcellular locationi

GO - Cellular componenti

  • cell periphery Source: GO_Central
  • compact myelin Source: RGD
  • internode region of axon Source: GO_Central
  • myelin sheath Source: UniProtKB
  • neuronal cell body Source: GO_Central
  • plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Keywords - Diseasei

Autoimmune encephalomyelitis

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 195194Myelin basic proteinPRO_0000158995Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei8 – 81PhosphoserineBy similarity
Modified residuei13 – 131PhosphoserineBy similarity
Modified residuei15 – 151PhosphotyrosineCombined sources
Modified residuei18 – 181PhosphothreonineCombined sources
Modified residuei20 – 201PhosphoserineCombined sources
Modified residuei21 – 211PhosphothreonineCombined sources
Modified residuei26 – 261CitrullineBy similarity
Modified residuei32 – 321CitrullineBy similarity
Modified residuei36 – 361PhosphothreonineCombined sources
Modified residuei41 – 411PhosphoserineCombined sources
Modified residuei57 – 571PhosphoserineBy similarity
Modified residuei92 – 921PhosphothreonineBy similarity
Modified residuei94 – 941PhosphotyrosineBy similarity
Modified residuei101 – 1011PhosphoserineBy similarity
Modified residuei104 – 1041PhosphothreonineBy similarity
Modified residuei119 – 1191Phosphothreonine1 Publication
Modified residuei122 – 1221PhosphothreonineBy similarity
Modified residuei127 – 1271Deamidated glutamineBy similarity
Modified residuei131 – 1311Omega-N-methylarginine; alternate1 Publication
Modified residuei131 – 1311Symmetric dimethylarginine; alternate1 Publication
Modified residuei139 – 1391PhosphoserineBy similarity
Modified residuei146 – 1461N6-acetyllysineBy similarity
Modified residuei154 – 1541CitrullineBy similarity
Modified residuei172 – 1721Deamidated glutamineBy similarity
Modified residuei184 – 1841CitrullineBy similarity
Modified residuei186 – 1861PhosphoserineBy similarity
Modified residuei190 – 1901Phosphoserine; by UHMK1By similarity
Modified residuei195 – 1951CitrullineBy similarity

Post-translational modificationi

As in other animals, several charge isomers may be produced as a result of optional post-translatonial modifications, such as phosphorylation of serine or threonine residues, deamidation of glutamine or asparagine residues, citrullination and methylation of arginine residues.2 Publications
Arg-131 was found to be 44% monomethylated and 11% symmetrically dimethylated.
Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1.By similarity

Keywords - PTMi

Acetylation, Citrullination, Methylation, Phosphoprotein

Proteomic databases

PaxDbiP02688.
PRIDEiP02688.

PTM databases

iPTMnetiP02688.
PhosphoSiteiP02688.
SwissPalmiP02688.

Miscellaneous databases

PMAP-CutDBP02688.

Expressioni

Tissue specificityi

Found in both the central and the peripheral nervous system.

Gene expression databases

ExpressionAtlasiP02688. baseline and differential.
GenevisibleiP02688. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Kcnma1Q629764EBI-1638296,EBI-1638146

Protein-protein interaction databases

BioGridi246698. 6 interactions.
IntActiP02688. 2 interactions.
MINTiMINT-248798.
STRINGi10116.ENSRNOP00000022303.

Family & Domainsi

Sequence similaritiesi

Belongs to the myelin basic protein family.Curated

Phylogenomic databases

eggNOGiENOG410IIUJ. Eukaryota.
ENOG4111PMJ. LUCA.
GeneTreeiENSGT00390000014772.
HOGENOMiHOG000293395.
HOVERGENiHBG008347.
InParanoidiP02688.
KOiK17269.
OMAiSEHKSAY.
OrthoDBiEOG7M0NRZ.

Family and domain databases

InterProiIPR000548. Myelin_BP.
[Graphical view]
PANTHERiPTHR11429. PTHR11429. 2 hits.
PfamiPF01669. Myelin_MBP. 1 hit.
[Graphical view]
PRINTSiPR00212. MYELINMBP.
PROSITEiPS00569. MYELIN_MBP. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P02688-1) [UniParc]FASTAAdd to basket

Also known as: 21.5 kDa

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASQKRPSQR HGSKYLATAS TMDHARHGFL PRHRDTGILD SIGRFFSGDR
60 70 80 90 100
GAPKRGSGKV PWLKQSRSPL PSHARSRPGL CHMYKDSHTR TTHYGSLPQK
110 120 130 140 150
SQRTQDENPV VHFFKNIVTP RTPPPSQGKG RGLSLSRFSW GAEGQKPGFG
160 170 180 190
YGGRASDYKS AHKGFKGAYD AQGTLSKIFK LGGRDSRSGS PMARR
Length:195
Mass (Da):21,502
Last modified:January 23, 2007 - v3
Checksum:iF1A43933EC9D4CFF
GO
Isoform 2 (identifier: P02688-2) [UniParc]FASTAAdd to basket

Also known as: 18.5 kDa, MBP L

The sequence of this isoform differs from the canonical sequence as follows:
     60-85: Missing.

Show »
Length:169
Mass (Da):18,488
Checksum:iB2D67AB198FC7D11
GO
Isoform 3 (identifier: P02688-3) [UniParc]FASTAAdd to basket

Also known as: 17 kDa

The sequence of this isoform differs from the canonical sequence as follows:
     141-181: Missing.

Show »
Length:154
Mass (Da):17,225
Checksum:iEFB722E97213E17A
GO
Isoform 4 (identifier: P02688-4) [UniParc]FASTAAdd to basket

Also known as: 14 kDa, MBP S, smaller myelin basic protein

The sequence of this isoform differs from the canonical sequence as follows:
     60-85: Missing.
     141-181: Missing.

Show »
Length:128
Mass (Da):14,211
Checksum:i2DAF033C19CF111C
GO
Isoform 5 (identifier: P02688-5) [UniParc]FASTAAdd to basket

Also known as: 17 kDa

The sequence of this isoform differs from the canonical sequence as follows:
     60-85: Missing.
     130-140: Missing.

Show »
Length:158
Mass (Da):17,240
Checksum:i3256580242ECC3E1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti47 – 482SG → GS AA sequence (PubMed:4122324).Curated
Sequence conflicti192 – 1921M → I in CAA10804 (Ref. 1) Curated
Sequence conflicti192 – 1921M → I in CAA10805 (Ref. 1) Curated
Sequence conflicti192 – 1921M → I in CAA10806 (Ref. 1) Curated
Sequence conflicti192 – 1921M → I in CAA10807 (Ref. 1) Curated
Sequence conflicti192 – 1921M → I (PubMed:6194889).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei60 – 8526Missing in isoform 2, isoform 4 and isoform 5. 5 PublicationsVSP_003321Add
BLAST
Alternative sequencei130 – 14011Missing in isoform 5. 1 PublicationVSP_025711Add
BLAST
Alternative sequencei141 – 18141Missing in isoform 3 and isoform 4. 4 PublicationsVSP_003322Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132895 mRNA. Translation: CAA10804.1.
AJ132896 mRNA. Translation: CAA10805.1.
AJ132897 mRNA. Translation: CAA10806.1.
AJ132898 mRNA. Translation: CAA10807.1.
M25889 mRNA. Translation: AAA41575.1.
K00512 mRNA. No translation available.
AF439750 mRNA. Translation: AAL84189.1.
BC094522 mRNA. Translation: AAH94522.1.
X72392 mRNA. No translation available.
PIRiB24351. MBRTS.
RefSeqiNP_001020462.1. NM_001025291.1. [P02688-1]
NP_001020463.1. NM_001025292.1. [P02688-2]
NP_001020464.1. NM_001025293.1. [P02688-3]
NP_001020465.1. NM_001025294.1. [P02688-5]
NP_058722.1. NM_017026.2. [P02688-4]
UniGeneiRn.203146.
Rn.63285.

Genome annotation databases

EnsembliENSRNOT00000022280; ENSRNOP00000022280; ENSRNOG00000016516. [P02688-3]
ENSRNOT00000022303; ENSRNOP00000022303; ENSRNOG00000016516. [P02688-1]
ENSRNOT00000058295; ENSRNOP00000055097; ENSRNOG00000016516. [P02688-2]
ENSRNOT00000058296; ENSRNOP00000055098; ENSRNOG00000016516. [P02688-5]
GeneIDi24547.
KEGGirno:24547.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132895 mRNA. Translation: CAA10804.1.
AJ132896 mRNA. Translation: CAA10805.1.
AJ132897 mRNA. Translation: CAA10806.1.
AJ132898 mRNA. Translation: CAA10807.1.
M25889 mRNA. Translation: AAA41575.1.
K00512 mRNA. No translation available.
AF439750 mRNA. Translation: AAL84189.1.
BC094522 mRNA. Translation: AAH94522.1.
X72392 mRNA. No translation available.
PIRiB24351. MBRTS.
RefSeqiNP_001020462.1. NM_001025291.1. [P02688-1]
NP_001020463.1. NM_001025292.1. [P02688-2]
NP_001020464.1. NM_001025293.1. [P02688-3]
NP_001020465.1. NM_001025294.1. [P02688-5]
NP_058722.1. NM_017026.2. [P02688-4]
UniGeneiRn.203146.
Rn.63285.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246698. 6 interactions.
IntActiP02688. 2 interactions.
MINTiMINT-248798.
STRINGi10116.ENSRNOP00000022303.

PTM databases

iPTMnetiP02688.
PhosphoSiteiP02688.
SwissPalmiP02688.

Proteomic databases

PaxDbiP02688.
PRIDEiP02688.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022280; ENSRNOP00000022280; ENSRNOG00000016516. [P02688-3]
ENSRNOT00000022303; ENSRNOP00000022303; ENSRNOG00000016516. [P02688-1]
ENSRNOT00000058295; ENSRNOP00000055097; ENSRNOG00000016516. [P02688-2]
ENSRNOT00000058296; ENSRNOP00000055098; ENSRNOG00000016516. [P02688-5]
GeneIDi24547.
KEGGirno:24547.

Organism-specific databases

CTDi4155.
RGDi3054. Mbp.

Phylogenomic databases

eggNOGiENOG410IIUJ. Eukaryota.
ENOG4111PMJ. LUCA.
GeneTreeiENSGT00390000014772.
HOGENOMiHOG000293395.
HOVERGENiHBG008347.
InParanoidiP02688.
KOiK17269.
OMAiSEHKSAY.
OrthoDBiEOG7M0NRZ.

Miscellaneous databases

PMAP-CutDBP02688.
PROiP02688.

Gene expression databases

ExpressionAtlasiP02688. baseline and differential.
GenevisibleiP02688. RN.

Family and domain databases

InterProiIPR000548. Myelin_BP.
[Graphical view]
PANTHERiPTHR11429. PTHR11429. 2 hits.
PfamiPF01669. Myelin_MBP. 1 hit.
[Graphical view]
PRINTSiPR00212. MYELINMBP.
PROSITEiPS00569. MYELIN_MBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Lobell A.M., Wigzell H.
    Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4).
  2. "Cloned proteolipid protein and myelin basic protein cDNA. Transcription of the two genes during myelination."
    Schaich M., Budzinski R.M., Stoffel W.
    Biol. Chem. Hoppe-Seyler 367:825-834(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
  3. "Characterization of cloned cDNA representing rat myelin basic protein: absence of expression in brain of shiverer mutant mice."
    Roach A., Boylan K.B., Horvath S., Prusiner S.B., Hood L.E.
    Cell 34:799-806(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
  4. "Identification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic protein."
    Matheus L., Blair G.E.
    Biochim. Biophys. Acta 1630:47-53(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5).
    Strain: Sprague-Dawley.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
    Tissue: Brain.
  6. "Amino acid sequence of the smaller basic protein from rat brain myelin."
    Dunkley P.R., Carnegie P.R.
    Biochem. J. 141:243-255(1974) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-195 (ISOFORM 4), CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, METHYLATION AT ARG-131.
    Tissue: Brain.
  7. Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 11-44; 68-75; 91-129; 138-154 AND 167-177, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus and Spinal cord.
  8. "Experimental allergic encephalomyelitis in the rat: response to encephalitogenic proteins and peptides."
    McFarlin D.E., Blank S.E., Kibler R.F., McKneally S.S., Shapira R.
    Science 179:478-480(1973) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 46-112 (ISOFORM 4).
  9. "Alternative splicing and cDNA sequence of myelin basic protein gene of the Lewis rat."
    Malotka J., Dornmair K.
    Autoimmunity 20:67-68(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 131-195.
    Strain: Lewis.
    Tissue: Brain.
  10. Lubec G., Chen W.-Q.
    Submitted (FEB-2007) to UniProtKB
    Cited for: PHOSPHORYLATION AT THR-119, IDENTIFICATION BY MASS SPECTROMETRY.
  11. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-15; THR-18; SER-20; THR-21; THR-36 AND SER-41, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMBP_RAT
AccessioniPrimary (citable) accession number: P02688
Secondary accession number(s): Q505J1
, Q8R4K6, Q9Z1J4, Q9Z1J5, Q9Z1J6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 149 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.