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Protein

Fibrinogen gamma chain

Gene

Fgg

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Together with fibrinogen alpha (FGA) and fibrinogen beta (FGB), polymerizes to form an insoluble fibrin matrix. Has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi344 – 3441CalciumBy similarity
Metal bindingi346 – 3461CalciumBy similarity
Metal bindingi350 – 3501Calcium; via carbonyl oxygenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Blood coagulation, Hemostasis

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-114608. Platelet degranulation.
R-RNO-140875. Common Pathway of Fibrin Clot Formation.
R-RNO-216083. Integrin cell surface interactions.
R-RNO-354192. Integrin alphaIIb beta3 signaling.
R-RNO-354194. GRB2:SOS provides linkage to MAPK signaling for Integrins.
R-RNO-372708. p130Cas linkage to MAPK signaling for integrins.

Names & Taxonomyi

Protein namesi
Recommended name:
Fibrinogen gamma chain
Gene namesi
Name:Fgg
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi2613. Fgg.

Subcellular locationi

  • Secreted By similarity

GO - Cellular componenti

  • blood microparticle Source: RGD
  • cell cortex Source: Ensembl
  • cytoplasm Source: RGD
  • external side of plasma membrane Source: Ensembl
  • extracellular exosome Source: Ensembl
  • fibrinogen complex Source: Ensembl
  • platelet alpha granule Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Add
BLAST
Chaini26 – 445420Fibrinogen gamma chainPRO_0000009101Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi34 – 34Interchain (with gamma chain)PROSITE-ProRule annotation
Disulfide bondi35 – 35Interchain (with gamma chain)PROSITE-ProRule annotation
Disulfide bondi45 – 45Interchain (with beta chain)PROSITE-ProRule annotation
Disulfide bondi49 – 49Interchain (with alpha chain)PROSITE-ProRule annotation
Glycosylationi78 – 781N-linked (GlcNAc...)Sequence analysis
Disulfide bondi161 – 161Interchain (with beta chain)PROSITE-ProRule annotation
Disulfide bondi165 – 165Interchain (with gamma chain)PROSITE-ProRule annotation
Disulfide bondi179 ↔ 208PROSITE-ProRule annotation
Disulfide bondi352 ↔ 365PROSITE-ProRule annotation
Cross-linki424 – 424Isoglutamyl lysine isopeptide (Gln-Lys) (interchain with K-432)By similarity
Modified residuei431 – 4311PhosphoserineCombined sources
Cross-linki432 – 432Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-424)By similarity

Post-translational modificationi

Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.

Keywords - PTMi

Disulfide bond, Glycoprotein, Isopeptide bond, Phosphoprotein

Proteomic databases

PaxDbiP02680.
PRIDEiP02680.

PTM databases

iPTMnetiP02680.

Expressioni

Gene expression databases

GenevisibleiP02680. RN.

Interactioni

Subunit structurei

Heterohexamer; disulfide linked. Contains 2 sets of 3 non-identical chains (alpha, beta and gamma). The 2 heterotrimers are in head to head conformation with the N-termini in a small central domain (By similarity).By similarity

Protein-protein interaction databases

IntActiP02680. 1 interaction.
STRINGi10116.ENSRNOP00000032735.

Structurei

3D structure databases

ProteinModelPortaliP02680.
SMRiP02680. Positions 30-424.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini170 – 416247Fibrinogen C-terminalPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni400 – 42223Gamma-chain polymerization, binding amino end of another fibrin alpha chainBy similarityAdd
BLAST

Domaini

A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.By similarity

Sequence similaritiesi

Contains 1 fibrinogen C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

eggNOGiKOG2579. Eukaryota.
ENOG410ZYS4. LUCA.
GeneTreeiENSGT00760000118809.
HOGENOMiHOG000037127.
HOVERGENiHBG099783.
InParanoidiP02680.
KOiK03905.
OMAiCHAGHLN.
OrthoDBiEOG7X9G60.
PhylomeDBiP02680.
TreeFamiTF336658.

Family and domain databases

Gene3Di3.90.215.10. 1 hit.
4.10.530.10. 1 hit.
InterProiIPR014716. Fibrinogen_a/b/g_C_1.
IPR014715. Fibrinogen_a/b/g_C_2.
IPR002181. Fibrinogen_a/b/g_C_dom.
IPR012290. Fibrinogen_a/b/g_coil_dom.
IPR020837. Fibrinogen_CS.
[Graphical view]
PfamiPF08702. Fib_alpha. 1 hit.
PF00147. Fibrinogen_C. 1 hit.
[Graphical view]
SMARTiSM00186. FBG. 1 hit.
SM01212. Fib_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF56496. SSF56496. 1 hit.
PROSITEiPS00514. FIBRINOGEN_C_1. 1 hit.
PS51406. FIBRINOGEN_C_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Gamma-B (identifier: P02680-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNWSLQLRSF ILCWALLLLS PTGLAQYTAT RDNCCILDER FGSYCPTTCG
60 70 80 90 100
ISDFLNSYQT DVDTDLQTLE NILQRAENRT TEAKELIKAI QVYYNPDQPP
110 120 130 140 150
KPGMIEGATQ KSKKMVEEIL KYEALLLTHE SSIRYLQDIY TSNKQKITNL
160 170 180 190 200
KQKVAQLEAQ CQEPCKDSVR IHDTTGKDCQ DIANKGAKES GLYFIRPLKA
210 220 230 240 250
TQQFLVYCEI DGSGNGWTVL QKRLDGSVDF KKNWIQYKEG FGHLSPTGTT
260 270 280 290 300
EFWLGNEKIH LISMQSTIPY ALRIQLKDWS GRTSTADYAM FRVGPESDKY
310 320 330 340 350
RLTYAYFIGG DAGDAFDGYD FGDDPSDKFF TSHNGMHFST WDNDNDKFEG
360 370 380 390 400
NCAEQDGSGW WMNKCHAGHL NGVYYQGGTY SKSSTPNGYD NGIIWATWKT
410 420 430 440
RWYSMKETTM KIIPFNRLSI GDGQQHHMGG SKQVSVEHEV DVEYP
Length:445
Mass (Da):50,633
Last modified:January 9, 2007 - v3
Checksum:iEB6F4FF7A7812054
GO
Isoform Gamma-A (identifier: P02680-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     435-437: SVE → GDM
     438-445: Missing.

Show »
Length:437
Mass (Da):49,652
Checksum:iDB58661DF73A0C06
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti160 – 1601Q → L in J00734 (PubMed:6897622).Curated
Sequence conflicti160 – 1601Q → L in CAA29289 (PubMed:3562236).Curated
Sequence conflicti162 – 1621Q → K in J00734 (PubMed:6897622).Curated
Sequence conflicti172 – 1743HDT → YDI in J00734 (PubMed:6897622).Curated
Sequence conflicti172 – 1743HDT → YDI in CAA29289 (PubMed:3562236).Curated
Sequence conflicti182 – 1821I → T in J00734 (PubMed:6897622).Curated
Sequence conflicti182 – 1821I → T in CAA29289 (PubMed:3562236).Curated
Sequence conflicti194 – 1941F → S in J00734 (PubMed:6897622).Curated
Sequence conflicti194 – 1941F → S in CAA29289 (PubMed:3562236).Curated
Sequence conflicti202 – 2021Q → E in J00734 (PubMed:6897622).Curated
Sequence conflicti202 – 2021Q → E in CAA29289 (PubMed:3562236).Curated
Sequence conflicti204 – 2041F → S in J00734 (PubMed:6897622).Curated
Sequence conflicti210 – 2101I → T in J00734 (PubMed:6897622).Curated
Sequence conflicti210 – 2101I → T in CAA29289 (PubMed:3562236).Curated
Sequence conflicti213 – 2131S → P in J00734 (PubMed:6897622).Curated
Sequence conflicti213 – 2131S → P in CAA29289 (PubMed:3562236).Curated
Sequence conflicti219 – 2213VLQ → EFK in J00734 (PubMed:6897622).Curated
Sequence conflicti219 – 2213VLQ → EFK in CAA29289 (PubMed:3562236).Curated
Sequence conflicti231 – 2311K → L in J00734 (PubMed:6897622).Curated
Sequence conflicti231 – 2311K → L in CAA29289 (PubMed:3562236).Curated
Sequence conflicti259 – 2591I → N in J00734 (PubMed:6897622).Curated
Sequence conflicti259 – 2591I → N in CAA29289 (PubMed:3562236).Curated
Sequence conflicti296 – 2961E → G in J00734 (PubMed:6897622).Curated
Sequence conflicti373 – 3731V → G in J00735 (PubMed:6897622).Curated
Sequence conflicti401 – 4011R → S in J00735 (PubMed:6897622).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei435 – 4373SVE → GDM in isoform Gamma-A. 2 PublicationsVSP_001538
Alternative sequencei438 – 4458Missing in isoform Gamma-A. 2 PublicationsVSP_001539

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00733 Genomic DNA. No translation available.
J00734 mRNA. No translation available.
J00735 mRNA. No translation available.
X05860, X05861 Genomic DNA. Translation: CAA29289.1.
BC078893 mRNA. Translation: AAH78893.1.
K01337 Genomic DNA. Translation: AAA98626.1.
PIRiA03128. FGRTGB.
A90828. FGRTGA.
RefSeqiNP_036691.2. NM_012559.2. [P02680-2]
XP_006232587.1. XM_006232525.1. [P02680-1]
UniGeneiRn.1702.

Genome annotation databases

EnsembliENSRNOT00000034521; ENSRNOP00000032735; ENSRNOG00000025074. [P02680-2]
GeneIDi24367.
KEGGirno:24367.
UCSCiRGD:2613. rat. [P02680-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00733 Genomic DNA. No translation available.
J00734 mRNA. No translation available.
J00735 mRNA. No translation available.
X05860, X05861 Genomic DNA. Translation: CAA29289.1.
BC078893 mRNA. Translation: AAH78893.1.
K01337 Genomic DNA. Translation: AAA98626.1.
PIRiA03128. FGRTGB.
A90828. FGRTGA.
RefSeqiNP_036691.2. NM_012559.2. [P02680-2]
XP_006232587.1. XM_006232525.1. [P02680-1]
UniGeneiRn.1702.

3D structure databases

ProteinModelPortaliP02680.
SMRiP02680. Positions 30-424.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP02680. 1 interaction.
STRINGi10116.ENSRNOP00000032735.

PTM databases

iPTMnetiP02680.

Proteomic databases

PaxDbiP02680.
PRIDEiP02680.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000034521; ENSRNOP00000032735; ENSRNOG00000025074. [P02680-2]
GeneIDi24367.
KEGGirno:24367.
UCSCiRGD:2613. rat. [P02680-1]

Organism-specific databases

CTDi2266.
RGDi2613. Fgg.

Phylogenomic databases

eggNOGiKOG2579. Eukaryota.
ENOG410ZYS4. LUCA.
GeneTreeiENSGT00760000118809.
HOGENOMiHOG000037127.
HOVERGENiHBG099783.
InParanoidiP02680.
KOiK03905.
OMAiCHAGHLN.
OrthoDBiEOG7X9G60.
PhylomeDBiP02680.
TreeFamiTF336658.

Enzyme and pathway databases

ReactomeiR-RNO-114608. Platelet degranulation.
R-RNO-140875. Common Pathway of Fibrin Clot Formation.
R-RNO-216083. Integrin cell surface interactions.
R-RNO-354192. Integrin alphaIIb beta3 signaling.
R-RNO-354194. GRB2:SOS provides linkage to MAPK signaling for Integrins.
R-RNO-372708. p130Cas linkage to MAPK signaling for integrins.

Miscellaneous databases

PROiP02680.

Gene expression databases

GenevisibleiP02680. RN.

Family and domain databases

Gene3Di3.90.215.10. 1 hit.
4.10.530.10. 1 hit.
InterProiIPR014716. Fibrinogen_a/b/g_C_1.
IPR014715. Fibrinogen_a/b/g_C_2.
IPR002181. Fibrinogen_a/b/g_C_dom.
IPR012290. Fibrinogen_a/b/g_coil_dom.
IPR020837. Fibrinogen_CS.
[Graphical view]
PfamiPF08702. Fib_alpha. 1 hit.
PF00147. Fibrinogen_C. 1 hit.
[Graphical view]
SMARTiSM00186. FBG. 1 hit.
SM01212. Fib_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF56496. SSF56496. 1 hit.
PROSITEiPS00514. FIBRINOGEN_C_1. 1 hit.
PS51406. FIBRINOGEN_C_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Organization of the rat gamma-fibrinogen gene: alternative mRNA splice patterns produce the gamma A and gamma B (gamma ') chains of fibrinogen."
    Crabtree G.R., Kant J.A.
    Cell 31:159-166(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS GAMMA-B AND GAMMA-A).
  2. "Nucleotide sequence of the gamma chain gene of rat fibrinogen: conserved intronic sequences."
    Morgan J.G., Holbrook N.J., Crabtree G.R.
    Nucleic Acids Res. 15:2774-2776(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM GAMMA-A).
    Tissue: Lung.
  4. "Potential basis for regulation of the coordinately expressed fibrinogen genes: homology in the 5' flanking regions."
    Fowlkes D.M., Mullis N.T., Comeau C.M., Crabtree G.R.
    Proc. Natl. Acad. Sci. U.S.A. 81:2313-2316(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-102.
  5. Lubec G., Afjehi-Sadat L.
    Submitted (NOV-2006) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 122-134, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Spinal cord.
  6. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-431, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFIBG_RAT
AccessioniPrimary (citable) accession number: P02680
Secondary accession number(s): Q68FY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 9, 2007
Last modified: June 8, 2016
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.