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P02668

- CASK_BOVIN

UniProt

P02668 - CASK_BOVIN

Protein

Kappa-casein

Gene

CSN3

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Kappa-casein stabilizes micelle formation, preventing casein precipitation in milk.
    Casoxins A, B and C have opioid antagonist activity. Casoxin C causes biphasic ileal contractions through the binding to the complement C3a receptors.
    Casoplatelin inhibits platelet aggregation.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei126 – 1272Cleavage; by chymosin/rennin

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. protein binding Source: IntAct

    GO - Biological processi

    1. lactation Source: Ensembl

    Keywords - Molecular functioni

    Milk protein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kappa-casein
    Cleaved into the following 5 chains:
    Gene namesi
    Name:CSN3
    Synonyms:CSN10, CSNK
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 6

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Protein family/group databases

    Allergomei10200. Bos d 12.0101.
    167. Bos d 8.
    2737. Bos d 12.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 21213 PublicationsAdd
    BLAST
    Chaini22 – 190169Kappa-caseinPRO_0000004483Add
    BLAST
    Peptidei46 – 5510Casoxin-CPRO_0000004484
    Peptidei54 – 596Casoxin-6PRO_0000004485
    Peptidei56 – 627Casoxin-APRO_0000004486
    Peptidei79 – 824Casoxin-BPRO_0000004487
    Peptidei127 – 13711CasoplatelinPRO_0000004488Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei22 – 221Pyrrolidone carboxylic acid
    Disulfide bondi32 ↔ 109
    Disulfide bondi32 – 32Interchain (with C-109); in linked form
    Disulfide bondi109 – 109Interchain (with C-32); in linked form
    Glycosylationi142 – 1421O-linked (GalNAc...)2 Publications
    Modified residuei148 – 1481Phosphoserine1 Publication
    Glycosylationi152 – 1521O-linked (GalNAc...)2 Publications
    Glycosylationi154 – 1541O-linked (GalNAc...)2 Publications
    Glycosylationi157 – 1571O-linked (GalNAc...)2 Publications
    Glycosylationi163 – 1631O-linked (GalNAc...)2 Publications
    Modified residuei170 – 1701Phosphoserine1 Publication
    Glycosylationi186 – 1861O-linked (GalNAc...); partial2 Publications
    Modified residuei187 – 1871PhosphoserineBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein, Pyrrolidone carboxylic acid

    Proteomic databases

    PaxDbiP02668.
    PRIDEiP02668.

    PTM databases

    UniCarbKBiP02668.

    Miscellaneous databases

    PMAP-CutDBP02668.

    Expressioni

    Tissue specificityi

    Mammary gland specific. Secreted in milk.

    Interactioni

    Subunit structurei

    Monomer or homomultimer; disulfide-linked.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-7234047,EBI-7234047
    clpBQ9RA633EBI-7234047,EBI-7698530From a different organism.

    Protein-protein interaction databases

    BioGridi159044. 2 interactions.
    IntActiP02668. 1 interaction.
    MINTiMINT-7258912.

    Structurei

    3D structure databases

    DisProtiDP00192.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the kappa-casein family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG40632.
    GeneTreeiENSGT00390000009184.
    HOVERGENiHBG005246.
    InParanoidiP02668.
    KOiK17282.
    OrthoDBiEOG7MWH09.
    TreeFamiTF338369.

    Family and domain databases

    InterProiIPR000117. Casein_kappa.
    [Graphical view]
    PANTHERiPTHR11470. PTHR11470. 1 hit.
    PfamiPF00997. Casein_kappa. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002374. Casein_kappa. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P02668-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMKSFFLVVT ILALTLPFLG AQEQNQEQPI RCEKDERFFS DKIAKYIPIQ    50
    YVLSRYPSYG LNYYQQKPVA LINNQFLPYP YYAKPAAVRS PAQILQWQVL 100
    SNTVPAKSCQ AQPTTMARHP HPHLSFMAIP PKKNQDKTEI PTINTIASGE 150
    PTSTPTTEAV ESTVATLEDS PEVIESPPEI NTVQVTSTAV 190
    Length:190
    Mass (Da):21,269
    Last modified:April 1, 1988 - v1
    Checksum:iF12D8310C3B93EDA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti23 – 231E → Q AA sequence 1 PublicationCurated
    Sequence conflicti26 – 261Q → E AA sequence 1 PublicationCurated
    Sequence conflicti28 – 281Q → E AA sequence 1 PublicationCurated
    Sequence conflicti102 – 1021N → D AA sequence (PubMed:4577852)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti31 – 311R → H in variant F.
    Natural varianti118 – 1181R → C in variant G.
    Natural varianti156 – 1561T → I in variant G and variant H.
    Natural varianti157 – 1571T → I in variant B and variant B2.
    Natural varianti169 – 1691D → A in variant B and variant B2.
    Natural varianti174 – 1741I → T in variant B2.
    Natural varianti176 – 1761S → G in variant E.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00565 mRNA. Translation: CAA25231.1.
    M36641 mRNA. Translation: AAA30433.1.
    X14907, X14908 Genomic DNA. Translation: CAA33034.1.
    AY380228 Genomic DNA. Translation: AAQ87922.1.
    AY380229 Genomic DNA. Translation: AAQ87923.1.
    BC102120 mRNA. Translation: AAI02121.1.
    K01085 mRNA. Translation: AAA30482.1.
    AF123250 Genomic DNA. Translation: AAD32139.1.
    AF123251 Genomic DNA. Translation: AAD32140.1.
    AF105260 Genomic DNA. Translation: AAF72097.1.
    M38333 mRNA. Translation: AAA30432.1.
    AF041482 Genomic DNA. Translation: AAB97519.1.
    U84250 Genomic DNA. Translation: AAB47260.1.
    U84251 Genomic DNA. Translation: AAB47261.1.
    PIRiS02076. KKBOB.
    RefSeqiNP_776719.1. NM_174294.2.
    UniGeneiBt.49421.

    Genome annotation databases

    EnsembliENSBTAT00000028685; ENSBTAP00000028685; ENSBTAG00000039787.
    GeneIDi281728.
    KEGGibta:281728.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Of buttons, digestion and glue - Issue 16 of November 2001

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00565 mRNA. Translation: CAA25231.1 .
    M36641 mRNA. Translation: AAA30433.1 .
    X14907 , X14908 Genomic DNA. Translation: CAA33034.1 .
    AY380228 Genomic DNA. Translation: AAQ87922.1 .
    AY380229 Genomic DNA. Translation: AAQ87923.1 .
    BC102120 mRNA. Translation: AAI02121.1 .
    K01085 mRNA. Translation: AAA30482.1 .
    AF123250 Genomic DNA. Translation: AAD32139.1 .
    AF123251 Genomic DNA. Translation: AAD32140.1 .
    AF105260 Genomic DNA. Translation: AAF72097.1 .
    M38333 mRNA. Translation: AAA30432.1 .
    AF041482 Genomic DNA. Translation: AAB97519.1 .
    U84250 Genomic DNA. Translation: AAB47260.1 .
    U84251 Genomic DNA. Translation: AAB47261.1 .
    PIRi S02076. KKBOB.
    RefSeqi NP_776719.1. NM_174294.2.
    UniGenei Bt.49421.

    3D structure databases

    DisProti DP00192.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 159044. 2 interactions.
    IntActi P02668. 1 interaction.
    MINTi MINT-7258912.

    Protein family/group databases

    Allergomei 10200. Bos d 12.0101.
    167. Bos d 8.
    2737. Bos d 12.

    PTM databases

    UniCarbKBi P02668.

    Proteomic databases

    PaxDbi P02668.
    PRIDEi P02668.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000028685 ; ENSBTAP00000028685 ; ENSBTAG00000039787 .
    GeneIDi 281728.
    KEGGi bta:281728.

    Organism-specific databases

    CTDi 1448.

    Phylogenomic databases

    eggNOGi NOG40632.
    GeneTreei ENSGT00390000009184.
    HOVERGENi HBG005246.
    InParanoidi P02668.
    KOi K17282.
    OrthoDBi EOG7MWH09.
    TreeFami TF338369.

    Miscellaneous databases

    NextBioi 20805648.
    PMAP-CutDB P02668.

    Family and domain databases

    InterProi IPR000117. Casein_kappa.
    [Graphical view ]
    PANTHERi PTHR11470. PTHR11470. 1 hit.
    Pfami PF00997. Casein_kappa. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002374. Casein_kappa. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequences of bovine alpha S1- and kappa-casein cDNAs."
      Stewart A.F., Willis I.M., Mackinlay A.G.
      Nucleic Acids Res. 12:3895-3907(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (VARIANT A).
    2. "Nucleotide sequence of the cDNA of kappa casein in cows."
      Gorodetskii S.I., Kaledin A.S.
      Genetika 23:596-604(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (VARIANT B2).
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (VARIANT A).
    4. "Polymorphism in the bovine kappa-casein (CSN3) gene and the 5'-flanking region: sequence analysis of CSN3 A and B alleles."
      Robitaille G., Britten M., Morisset J., Petitclerc D.
      Anim. Genet. 36:184-185(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (VARIANTS A AND B).
      Tissue: Blood.
    5. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (VARIANT B).
      Strain: Hereford.
      Tissue: Mammary gland.
    6. "Primary structure of bovine kappa B casein. Complete sequence."
      Mercier J.-C., Brignon G., Ribadeau-Dumas B.
      Eur. J. Biochem. 35:222-235(1973) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 22-127 (VARIANT B).
    7. "Studies on the primary structure of cow kappa-casein. The primary sequence of cow para-kappa-casein."
      Jolles J., Schoentgen F., Alais C., Jolles P.
      Chimia 26:645-646(1972)
      Cited for: PROTEIN SEQUENCE OF 22-126 (VARIANT A).
    8. "The multimeric structure and disulfide-bonding pattern of bovine kappa-casein."
      Rasmussen L.K., Hoejrup P., Petersen T.E.
      Eur. J. Biochem. 207:215-222(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 22-38 AND 97-116, INTERCHAIN DISULFIDE BONDS, IDENTIFICATION BY MASS SPECTROMETRY.
    9. "Construction and identification by partial nucleotide sequence analysis of bovine casein and beta-lactoglobulin cDNA clones."
      Willis I.M., Stewart A.F., Caputo A., Thompson A.R., McKinlay A.G.
      DNA 1:375-386(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 31-45.
    10. "Molecular genetic characterization of new bovine kappa-casein alleles CSN3F and CSN3G and genotyping by PCR-RFLP."
      Prinzenberg E.M., Hiendleder S., Ikonen T., Erhardt G.
      Anim. Genet. 27:347-349(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 31-190 (VARIANTS F AND G).
      Strain: Ayrshire and Pinzgauer.
    11. "SSCP analysis at the bovine CSN3 locus discriminates six alleles corresponding to known protein variants (A, B, C, E, F, G) and three new DNA polymorphisms (H, I, A1)."
      Prinzenberg E.M., Krause I., Erhardt G.
      Anim. Biotechnol. 10:49-62(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-190 (VARIANT H).
      Strain: Pinzgauer.
    12. "Specificity of hydrolysis of bovine kappa-casein by cell envelope-associated proteinases from Lactococcus lactis strains."
      Reid J.R., Coolbear T., Pillidge C.J., Pritchard G.G.
      Appl. Environ. Microbiol. 60:801-806(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 54-59.
    13. "Primary structure of cDNA of Bos taurus kappa-casein macropeptide."
      Gorodetskii S.I., Kershulyte D.R., Korobko V.G.
      Bioorg. Khim. 9:1693-1695(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 92-190 (VARIANT B2).
    14. "Studies on the primary structure of cow kappa-casein. Structural features of para-kappa-casein; N-terminal sequence of kappa-caseinoglycopeptide studied with a sequencer."
      Jolles J., Schoentgen F., Alais C., Fiat A.-M., Jolles P.
      Helv. Chim. Acta 55:2872-2883(1972) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE (VARIANT A).
    15. "Kappa-casein from bovine colostrum."
      Guerin J., Alais C., Jolles J., Jolles P.
      Biochim. Biophys. Acta 351:325-332(1974) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 127-168 AND 187-190.
      Tissue: Colostrum.
    16. "Localization of amino-acid substitutions that differenciate bovine kappa-casein variants A and B."
      Grosclaude F., Mahe M.-F., Mercier J.-C., Ribadeau-Dumas B.
      Ann. Genet. Sel. Anim. 4:515-521(1972)
      Cited for: PROTEIN SEQUENCE OF 128-190 (VARIANTS A AND B).
    17. "Genotyping of bovine kappa-casein (kappa-CNA, kappa-CNB, kappa-CNC, kappa-CNE) following DNA sequence amplification and direct sequencing of kappa-CNE PCR product."
      Schlieben S., Erhardt G., Senft B.
      Anim. Genet. 22:333-342(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 133-190 (VARIANT E).
    18. Woollard J.R., Dentine M.R.
      Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE OF 133-190 (VARIANTS A AND B).
    19. "Characterization of O-linked glycosylation motifs in the glycopeptide domain of bovine kappa-casein."
      Pisano A., Packer N.H., Redmond J.W., Williams K.L., Gooley A.A.
      Glycobiology 4:837-844(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT THR-142; THR-152; THR-154; THR-157; THR-163 AND THR-186.
    20. "Reversed-phase high-performance liquid chromatographic separation of bovine kappa-casein macropeptide and characterization of isolated fractions."
      Minkiewicz P., Slangen C.J., Lagerwerf F.M., Haverkamp J., Rollema H.S., Visser S.
      J. Chromatogr. A 743:123-135(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT THR-142; THR-152; THR-154; THR-157; THR-163 AND THR-186, PHOSPHORYLATION AT SER-148 AND SER-170.
    21. "Opioid antagonist peptides derived from kappa-casein."
      Chiba H., Tani F., Yoshikawa M.
      J. Dairy Res. 56:363-366(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACTIVITY OF CASOXINS.
    22. "Analogy between fibrinogen and casein. Effect of an undecapeptide isolated from kappa-casein on platelet function."
      Jolles P., Levy-Toledano S., Fiat A.-M., Soria C., Gillessen D., Thomaidis A., Dunn F.W., Caen J.P.
      Eur. J. Biochem. 158:379-382(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACTIVITY OF CASOPLATELIN.

    Entry informationi

    Entry nameiCASK_BOVIN
    AccessioniPrimary (citable) accession number: P02668
    Secondary accession number(s): O46566
    , Q597F3, Q6U205, Q9N271, Q9TRQ3, Q9TV96, Q9TV97
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: April 1, 1988
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The sequence shown is the A variant.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3