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P02668

- CASK_BOVIN

UniProt

P02668 - CASK_BOVIN

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Protein

Kappa-casein

Gene
CSN3, CSN10, CSNK
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Kappa-casein stabilizes micelle formation, preventing casein precipitation in milk.
Casoxins A, B and C have opioid antagonist activity. Casoxin C causes biphasic ileal contractions through the binding to the complement C3a receptors.
Casoplatelin inhibits platelet aggregation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei126 – 1272Cleavage; by chymosin/rennin

GO - Molecular functioni

  1. identical protein binding Source: IntAct
  2. protein binding Source: IntAct

GO - Biological processi

  1. lactation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Milk protein

Names & Taxonomyi

Protein namesi
Recommended name:
Kappa-casein
Cleaved into the following 5 chains:
Gene namesi
Name:CSN3
Synonyms:CSN10, CSNK
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 6

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei10200. Bos d 12.0101.
167. Bos d 8.
2737. Bos d 12.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21213 PublicationsAdd
BLAST
Chaini22 – 190169Kappa-caseinPRO_0000004483Add
BLAST
Peptidei46 – 5510Casoxin-CPRO_0000004484
Peptidei54 – 596Casoxin-61 PublicationPRO_0000004485
Peptidei56 – 627Casoxin-APRO_0000004486
Peptidei79 – 824Casoxin-BPRO_0000004487
Peptidei127 – 13711CasoplatelinPRO_0000004488Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei22 – 221Pyrrolidone carboxylic acid
Disulfide bondi32 ↔ 109
Disulfide bondi32 – 32Interchain (with C-109); in linked form
Disulfide bondi109 – 109Interchain (with C-32); in linked form
Glycosylationi142 – 1421O-linked (GalNAc...)2 Publications
Modified residuei148 – 1481Phosphoserine1 Publication
Glycosylationi152 – 1521O-linked (GalNAc...)2 Publications
Glycosylationi154 – 1541O-linked (GalNAc...)2 Publications
Glycosylationi157 – 1571O-linked (GalNAc...)2 Publications
Glycosylationi163 – 1631O-linked (GalNAc...)2 Publications
Modified residuei170 – 1701Phosphoserine1 Publication
Glycosylationi186 – 1861O-linked (GalNAc...); partial2 Publications
Modified residuei187 – 1871Phosphoserine By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP02668.
PRIDEiP02668.

PTM databases

UniCarbKBiP02668.

Miscellaneous databases

PMAP-CutDBP02668.

Expressioni

Tissue specificityi

Mammary gland specific. Secreted in milk.

Interactioni

Subunit structurei

Monomer or homomultimer; disulfide-linked.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-7234047,EBI-7234047
clpBQ9RA633EBI-7234047,EBI-7698530From a different organism.

Protein-protein interaction databases

BioGridi159044. 2 interactions.
IntActiP02668. 1 interaction.
MINTiMINT-7258912.

Structurei

3D structure databases

DisProtiDP00192.

Family & Domainsi

Sequence similaritiesi

Belongs to the kappa-casein family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG40632.
GeneTreeiENSGT00390000009184.
HOVERGENiHBG005246.
InParanoidiP02668.
KOiK17282.
OrthoDBiEOG7MWH09.
TreeFamiTF338369.

Family and domain databases

InterProiIPR000117. Casein_kappa.
[Graphical view]
PANTHERiPTHR11470. PTHR11470. 1 hit.
PfamiPF00997. Casein_kappa. 1 hit.
[Graphical view]
PIRSFiPIRSF002374. Casein_kappa. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02668-1 [UniParc]FASTAAdd to Basket

« Hide

MMKSFFLVVT ILALTLPFLG AQEQNQEQPI RCEKDERFFS DKIAKYIPIQ    50
YVLSRYPSYG LNYYQQKPVA LINNQFLPYP YYAKPAAVRS PAQILQWQVL 100
SNTVPAKSCQ AQPTTMARHP HPHLSFMAIP PKKNQDKTEI PTINTIASGE 150
PTSTPTTEAV ESTVATLEDS PEVIESPPEI NTVQVTSTAV 190
Length:190
Mass (Da):21,269
Last modified:April 1, 1988 - v1
Checksum:iF12D8310C3B93EDA
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti31 – 311R → H in variant F.
Natural varianti118 – 1181R → C in variant G.
Natural varianti156 – 1561T → I in variant G and variant H.
Natural varianti157 – 1571T → I in variant B and variant B2.
Natural varianti169 – 1691D → A in variant B and variant B2.
Natural varianti174 – 1741I → T in variant B2.
Natural varianti176 – 1761S → G in variant E.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti23 – 231E → Q AA sequence 1 Publication
Sequence conflicti26 – 261Q → E AA sequence 1 Publication
Sequence conflicti28 – 281Q → E AA sequence 1 Publication
Sequence conflicti102 – 1021N → D AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X00565 mRNA. Translation: CAA25231.1.
M36641 mRNA. Translation: AAA30433.1.
X14907, X14908 Genomic DNA. Translation: CAA33034.1.
AY380228 Genomic DNA. Translation: AAQ87922.1.
AY380229 Genomic DNA. Translation: AAQ87923.1.
BC102120 mRNA. Translation: AAI02121.1.
K01085 mRNA. Translation: AAA30482.1.
AF123250 Genomic DNA. Translation: AAD32139.1.
AF123251 Genomic DNA. Translation: AAD32140.1.
AF105260 Genomic DNA. Translation: AAF72097.1.
M38333 mRNA. Translation: AAA30432.1.
AF041482 Genomic DNA. Translation: AAB97519.1.
U84250 Genomic DNA. Translation: AAB47260.1.
U84251 Genomic DNA. Translation: AAB47261.1.
PIRiS02076. KKBOB.
RefSeqiNP_776719.1. NM_174294.2.
UniGeneiBt.49421.

Genome annotation databases

EnsembliENSBTAT00000028685; ENSBTAP00000028685; ENSBTAG00000039787.
GeneIDi281728.
KEGGibta:281728.

Cross-referencesi

Web resourcesi

Protein Spotlight

Of buttons, digestion and glue - Issue 16 of November 2001

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X00565 mRNA. Translation: CAA25231.1 .
M36641 mRNA. Translation: AAA30433.1 .
X14907 , X14908 Genomic DNA. Translation: CAA33034.1 .
AY380228 Genomic DNA. Translation: AAQ87922.1 .
AY380229 Genomic DNA. Translation: AAQ87923.1 .
BC102120 mRNA. Translation: AAI02121.1 .
K01085 mRNA. Translation: AAA30482.1 .
AF123250 Genomic DNA. Translation: AAD32139.1 .
AF123251 Genomic DNA. Translation: AAD32140.1 .
AF105260 Genomic DNA. Translation: AAF72097.1 .
M38333 mRNA. Translation: AAA30432.1 .
AF041482 Genomic DNA. Translation: AAB97519.1 .
U84250 Genomic DNA. Translation: AAB47260.1 .
U84251 Genomic DNA. Translation: AAB47261.1 .
PIRi S02076. KKBOB.
RefSeqi NP_776719.1. NM_174294.2.
UniGenei Bt.49421.

3D structure databases

DisProti DP00192.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 159044. 2 interactions.
IntActi P02668. 1 interaction.
MINTi MINT-7258912.

Protein family/group databases

Allergomei 10200. Bos d 12.0101.
167. Bos d 8.
2737. Bos d 12.

PTM databases

UniCarbKBi P02668.

Proteomic databases

PaxDbi P02668.
PRIDEi P02668.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000028685 ; ENSBTAP00000028685 ; ENSBTAG00000039787 .
GeneIDi 281728.
KEGGi bta:281728.

Organism-specific databases

CTDi 1448.

Phylogenomic databases

eggNOGi NOG40632.
GeneTreei ENSGT00390000009184.
HOVERGENi HBG005246.
InParanoidi P02668.
KOi K17282.
OrthoDBi EOG7MWH09.
TreeFami TF338369.

Miscellaneous databases

NextBioi 20805648.
PMAP-CutDB P02668.

Family and domain databases

InterProi IPR000117. Casein_kappa.
[Graphical view ]
PANTHERi PTHR11470. PTHR11470. 1 hit.
Pfami PF00997. Casein_kappa. 1 hit.
[Graphical view ]
PIRSFi PIRSF002374. Casein_kappa. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequences of bovine alpha S1- and kappa-casein cDNAs."
    Stewart A.F., Willis I.M., Mackinlay A.G.
    Nucleic Acids Res. 12:3895-3907(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (VARIANT A).
  2. "Nucleotide sequence of the cDNA of kappa casein in cows."
    Gorodetskii S.I., Kaledin A.S.
    Genetika 23:596-604(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (VARIANT B2).
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (VARIANT A).
  4. "Polymorphism in the bovine kappa-casein (CSN3) gene and the 5'-flanking region: sequence analysis of CSN3 A and B alleles."
    Robitaille G., Britten M., Morisset J., Petitclerc D.
    Anim. Genet. 36:184-185(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (VARIANTS A AND B).
    Tissue: Blood.
  5. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (VARIANT B).
    Strain: Hereford.
    Tissue: Mammary gland.
  6. "Primary structure of bovine kappa B casein. Complete sequence."
    Mercier J.-C., Brignon G., Ribadeau-Dumas B.
    Eur. J. Biochem. 35:222-235(1973) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 22-127 (VARIANT B).
  7. "Studies on the primary structure of cow kappa-casein. The primary sequence of cow para-kappa-casein."
    Jolles J., Schoentgen F., Alais C., Jolles P.
    Chimia 26:645-646(1972)
    Cited for: PROTEIN SEQUENCE OF 22-126 (VARIANT A).
  8. "The multimeric structure and disulfide-bonding pattern of bovine kappa-casein."
    Rasmussen L.K., Hoejrup P., Petersen T.E.
    Eur. J. Biochem. 207:215-222(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 22-38 AND 97-116, INTERCHAIN DISULFIDE BONDS, IDENTIFICATION BY MASS SPECTROMETRY.
  9. "Construction and identification by partial nucleotide sequence analysis of bovine casein and beta-lactoglobulin cDNA clones."
    Willis I.M., Stewart A.F., Caputo A., Thompson A.R., McKinlay A.G.
    DNA 1:375-386(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 31-45.
  10. "Molecular genetic characterization of new bovine kappa-casein alleles CSN3F and CSN3G and genotyping by PCR-RFLP."
    Prinzenberg E.M., Hiendleder S., Ikonen T., Erhardt G.
    Anim. Genet. 27:347-349(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 31-190 (VARIANTS F AND G).
    Strain: Ayrshire and Pinzgauer.
  11. "SSCP analysis at the bovine CSN3 locus discriminates six alleles corresponding to known protein variants (A, B, C, E, F, G) and three new DNA polymorphisms (H, I, A1)."
    Prinzenberg E.M., Krause I., Erhardt G.
    Anim. Biotechnol. 10:49-62(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-190 (VARIANT H).
    Strain: Pinzgauer.
  12. "Specificity of hydrolysis of bovine kappa-casein by cell envelope-associated proteinases from Lactococcus lactis strains."
    Reid J.R., Coolbear T., Pillidge C.J., Pritchard G.G.
    Appl. Environ. Microbiol. 60:801-806(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 54-59.
  13. "Primary structure of cDNA of Bos taurus kappa-casein macropeptide."
    Gorodetskii S.I., Kershulyte D.R., Korobko V.G.
    Bioorg. Khim. 9:1693-1695(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 92-190 (VARIANT B2).
  14. "Studies on the primary structure of cow kappa-casein. Structural features of para-kappa-casein; N-terminal sequence of kappa-caseinoglycopeptide studied with a sequencer."
    Jolles J., Schoentgen F., Alais C., Fiat A.-M., Jolles P.
    Helv. Chim. Acta 55:2872-2883(1972) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE (VARIANT A).
  15. "Kappa-casein from bovine colostrum."
    Guerin J., Alais C., Jolles J., Jolles P.
    Biochim. Biophys. Acta 351:325-332(1974) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 127-168 AND 187-190.
    Tissue: Colostrum.
  16. "Localization of amino-acid substitutions that differenciate bovine kappa-casein variants A and B."
    Grosclaude F., Mahe M.-F., Mercier J.-C., Ribadeau-Dumas B.
    Ann. Genet. Sel. Anim. 4:515-521(1972)
    Cited for: PROTEIN SEQUENCE OF 128-190 (VARIANTS A AND B).
  17. "Genotyping of bovine kappa-casein (kappa-CNA, kappa-CNB, kappa-CNC, kappa-CNE) following DNA sequence amplification and direct sequencing of kappa-CNE PCR product."
    Schlieben S., Erhardt G., Senft B.
    Anim. Genet. 22:333-342(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 133-190 (VARIANT E).
  18. Woollard J.R., Dentine M.R.
    Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE OF 133-190 (VARIANTS A AND B).
  19. "Characterization of O-linked glycosylation motifs in the glycopeptide domain of bovine kappa-casein."
    Pisano A., Packer N.H., Redmond J.W., Williams K.L., Gooley A.A.
    Glycobiology 4:837-844(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT THR-142; THR-152; THR-154; THR-157; THR-163 AND THR-186.
  20. "Reversed-phase high-performance liquid chromatographic separation of bovine kappa-casein macropeptide and characterization of isolated fractions."
    Minkiewicz P., Slangen C.J., Lagerwerf F.M., Haverkamp J., Rollema H.S., Visser S.
    J. Chromatogr. A 743:123-135(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT THR-142; THR-152; THR-154; THR-157; THR-163 AND THR-186, PHOSPHORYLATION AT SER-148 AND SER-170.
  21. "Opioid antagonist peptides derived from kappa-casein."
    Chiba H., Tani F., Yoshikawa M.
    J. Dairy Res. 56:363-366(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACTIVITY OF CASOXINS.
  22. "Analogy between fibrinogen and casein. Effect of an undecapeptide isolated from kappa-casein on platelet function."
    Jolles P., Levy-Toledano S., Fiat A.-M., Soria C., Gillessen D., Thomaidis A., Dunn F.W., Caen J.P.
    Eur. J. Biochem. 158:379-382(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACTIVITY OF CASOPLATELIN.

Entry informationi

Entry nameiCASK_BOVIN
AccessioniPrimary (citable) accession number: P02668
Secondary accession number(s): O46566
, Q597F3, Q6U205, Q9N271, Q9TRQ3, Q9TV96, Q9TV97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 1, 1988
Last modified: September 3, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The sequence shown is the A variant.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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