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Protein

Beta-casein

Gene

CSN2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Important role in determination of the surface properties of the casein micelles.
Casoparan acts as a macrophage activator, increasing the phagocytic activity of macrophages and peroxide release from macrophages. It also acts as a bradykinin-potentiating peptide.
Casohypotensin acts as a bradykinin-potentiating peptide. Induces hypotension in rats. Acts as a strong competitive inhibitor of endo-oligopeptidase A.
Antioxidant peptide has antioxidant activity.

GO - Molecular functioni

  • antioxidant activity Source: UniProtKB-KW
  • calcium ion binding Source: GO_Central
  • cysteine-type endopeptidase inhibitor activity Source: CAFA
  • metalloendopeptidase inhibitor activity Source: UniProtKB-KW
  • potassium channel inhibitor activity Source: AgBase
  • protein homodimerization activity Source: AgBase
  • transporter activity Source: InterPro

GO - Biological processi

  • calcium ion transport Source: GO_Central
  • lactation Source: Ensembl
  • negative regulation of cysteine-type endopeptidase activity Source: CAFA
  • negative regulation of I-kappaB kinase/NF-kappaB signaling Source: CAFA
  • negative regulation of I-kappaB phosphorylation Source: CAFA
  • negative regulation of inflammatory response Source: CAFA
  • negative regulation of lactation Source: AgBase
  • negative regulation of tumor necrosis factor-mediated signaling pathway Source: CAFA
  • regulation of blood pressure Source: UniProtKB-KW
  • response to 11-deoxycorticosterone Source: AgBase
  • response to dehydroepiandrosterone Source: AgBase
  • response to estradiol Source: AgBase
  • response to heat Source: AgBase
  • response to progesterone Source: AgBase

Keywordsi

Molecular functionAntioxidant, Hypotensive agent, Metalloenzyme inhibitor, Metalloprotease inhibitor, Milk protein, Protease inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-casein
Cleaved into the following 3 chains:
Gene namesi
Name:CSN2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei10199. Bos d 11.0101.
167. Bos d 8.
2736. Bos d 11.

Chemistry databases

ChEMBLiCHEMBL3313833.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 153 PublicationsAdd BLAST15
ChainiPRO_000000447016 – 224Beta-caseinAdd BLAST209
PeptideiPRO_000029203141 – 45Casoparan5
PeptideiPRO_0000320153113 – 120Antioxidant peptide8
PeptideiPRO_0000308464129 – 136Casohypotensin8

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei30Phosphoserine3 Publications1
Modified residuei32Phosphoserine3 Publications1
Modified residuei33Phosphoserine3 Publications1
Modified residuei34Phosphoserine3 Publications1
Modified residuei50Phosphoserine; in variant A1, variant A2, variant A3, variant B, variant E, variant F, variant G and variant H2 Publications1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP02666.
PeptideAtlasiP02666.
PRIDEiP02666.

PTM databases

iPTMnetiP02666.

Miscellaneous databases

PMAP-CutDBiP02666.

Expressioni

Tissue specificityi

Mammary gland specific. Secreted in milk.

Gene expression databases

BgeeiENSBTAG00000002632.

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein homodimerization activity Source: AgBase

Protein-protein interaction databases

DIPiDIP-46257N.
IntActiP02666. 4 interactors.
STRINGi9913.ENSBTAP00000003409.

Chemistry databases

BindingDBiP02666.

Structurei

3D structure databases

DisProtiDP00329.
ProteinModelPortaliP02666.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the beta-casein family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IYJE. Eukaryota.
ENOG411197Z. LUCA.
GeneTreeiENSGT00390000001890.
HOVERGENiHBG004973.
InParanoidiP02666.
OMAiEIMEVPK.
OrthoDBiEOG091G0XI8.
TreeFamiTF336929.

Family and domain databases

InterProiView protein in InterPro
IPR001588. Casein.
IPR016345. Casein_beta.
IPR031305. Casein_CS.
PANTHERiPTHR11500. PTHR11500. 1 hit.
PfamiView protein in Pfam
PF00363. Casein. 1 hit.
PIRSFiPIRSF002372. Beta-casein. 1 hit.
PROSITEiView protein in PROSITE
PS00306. CASEIN_ALPHA_BETA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02666-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVLILACLV ALALARELEE LNVPGEIVES LSSSEESITR INKKIEKFQS
60 70 80 90 100
EEQQQTEDEL QDKIHPFAQT QSLVYPFPGP IPNSLPQNIP PLTQTPVVVP
110 120 130 140 150
PFLQPEVMGV SKVKEAMAPK HKEMPFPKYP VEPFTESQSL TLTDVENLHL
160 170 180 190 200
PLPLLQSWMH QPHQPLPPTV MFPPQSVLSL SQSKVLPVPQ KAVPYPQRDM
210 220
PIQAFLLYQE PVLGPVRGPF PIIV
Length:224
Mass (Da):25,107
Last modified:July 1, 1989 - v2
Checksum:iF0BBDD8148A238AE
GO

Sequence cautioni

The sequence AAW84270 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAW84271 differs from that shown. Reason: Erroneous initiation.Curated
The sequence ABL74247 differs from that shown. Reason: Frameshift at positions 65 and 71.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50S → Z AA sequence (PubMed:1804413).Curated1
Sequence conflicti69Q → R in ABL74247 (Ref. 14) Curated1
Sequence conflicti112K → R in ABL74247 (Ref. 14) Curated1
Sequence conflicti208Y → V in CAC37028 (Ref. 16) Curated1
Sequence conflicti209 – 210QE → EQ AA sequence (PubMed:1804413).Curated2
Sequence conflicti210E → Q in AAA30430 (PubMed:3814153).Curated1
Sequence conflicti210E → Q no nucleotide entry (PubMed:4557764).Curated1
Sequence conflicti212V → A in ABR10906 (Ref. 15) Curated1

Mass spectrometryi

Molecular mass is 872.51 Da from positions 113 - 120. Determined by ESI. 1 Publication

Polymorphismi

Leu-152 is present in the variants F and G; Gln-190 and Glu-210 are present in the variant H. The sequence shown is the A2 variant.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti33S → K in variant D. 1 Publication1
Natural varianti40R → C in variant H. 1
Natural varianti51E → K in variant E. 1 Publication1
Natural varianti52E → K in variant C. 1
Natural varianti82P → H in variants A1, B, C, F and G. 3 Publications1
Natural varianti103L → I in variant H. 1
Natural varianti108M → L4 Publications1
Natural varianti121H → Q in variant A3. 2 Publications1
Natural varianti132E → Q in variants A1 and G. 2 Publications1
Natural varianti137S → R in variant B. 1 Publication1
Natural varianti152L → P in variants A1 and H. 1 Publication1
Natural varianti153P → L in variants A1, G and H. 1 Publication1
Natural varianti167P → L in variant F. 1
Natural varianti190Q → E in variants A1 and G. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15132 mRNA. Translation: AAA30430.1.
X06359 mRNA. Translation: CAA29658.1.
M16645 mRNA. Translation: AAA30480.1.
M55158 Genomic DNA. Translation: AAA30431.1.
S67277 mRNA. Translation: AAB29137.1.
BC111172 mRNA. Translation: AAI11173.1.
AY899917 mRNA. Translation: AAW84270.1. Different initiation.
AY899918 mRNA. Translation: AAW84271.1. Different initiation.
AH007287 Genomic DNA. Translation: AAD09813.1.
EF123100 Genomic DNA. Translation: ABL74247.1. Frameshift.
EF628290 Genomic DNA. Translation: ABR10906.1.
AJ296330 Genomic DNA. Translation: CAC37028.1.
M64756 mRNA. Translation: AAB59254.1.
AY366419 Genomic DNA. Translation: AAR14677.1.
K01087 mRNA. Translation: AAA30481.1.
PIRiA59068.
I45873. KBBOA2.
UniGeneiBt.53272.

Genome annotation databases

EnsembliENSBTAT00000003409; ENSBTAP00000003409; ENSBTAG00000002632.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCASB_BOVIN
AccessioniPrimary (citable) accession number: P02666
Secondary accession number(s): A1YQZ8
, A6N8V0, Q2TA13, Q5EEQ6, Q5EEQ7, Q6UN63, Q9BDG5, Q9TSD5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1989
Last modified: November 22, 2017
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families