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P02666

- CASB_BOVIN

UniProt

P02666 - CASB_BOVIN

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Protein
Beta-casein
Gene
CSN2
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Important role in determination of the surface properties of the casein micelles.4 Publications
Casoparan acts as a macrophage activator, increasing the phagocytic activity of macrophages and peroxide release from macrophages. It also acts as a bradykinin-potentiating peptide.4 Publications
Casohypotensin acts as a bradykinin-potentiating peptide. Induces hypotension in rats. Acts as a strong competitive inhibitor of endo-oligopeptidase A.4 Publications
Antioxidant peptide has antioxidant activity.4 Publications

GO - Molecular functioni

  1. antioxidant activity Source: UniProtKB-KW
  2. metalloendopeptidase inhibitor activity Source: UniProtKB-KW
  3. protein binding Source: IntAct
  4. transporter activity Source: InterPro

GO - Biological processi

  1. regulation of blood pressure Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Antioxidant, Hypotensive agent, Metalloenzyme inhibitor, Metalloprotease inhibitor, Milk protein, Protease inhibitor

Enzyme and pathway databases

ReactomeiREACT_220588. Nuclear signaling by ERBB4.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-casein
Cleaved into the following 3 chains:
Gene namesi
Name:CSN2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 6

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei10199. Bos d 11.0101.
167. Bos d 8.
2736. Bos d 11.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 15153 Publications
Add
BLAST
Chaini16 – 224209Beta-casein
PRO_0000004470Add
BLAST
Peptidei41 – 455Casoparan1 Publication
PRO_0000292031
Peptidei113 – 1208Antioxidant peptide1 Publication
PRO_0000320153
Peptidei129 – 1368Casohypotensin2 Publications
PRO_0000308464

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei30 – 301Phosphoserine3 Publications
Modified residuei32 – 321Phosphoserine3 Publications
Modified residuei33 – 331Phosphoserine3 Publications
Modified residuei34 – 341Phosphoserine3 Publications
Modified residuei50 – 501Phosphoserine; in variant A1, variant A2, variant A3, variant B, variant E, variant F, variant G and variant H2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP02666.
PRIDEiP02666.

Miscellaneous databases

PMAP-CutDBP02666.

Expressioni

Tissue specificityi

Mammary gland specific. Secreted in milk.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
HTRA2O434645EBI-5260183,EBI-517086From a different organism.
LONP1P367766EBI-5260183,EBI-357448From a different organism.

Protein-protein interaction databases

BioGridi158462. 1 interaction.
DIPiDIP-46257N.
IntActiP02666. 2 interactions.
STRINGi9913.ENSBTAP00000003409.

Structurei

3D structure databases

DisProtiDP00329.
ProteinModelPortaliP02666.

Family & Domainsi

Sequence similaritiesi

Belongs to the beta-casein family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG45871.
GeneTreeiENSGT00390000001890.
HOVERGENiHBG004973.
InParanoidiP02666.
OMAiEIMEVPK.
OrthoDBiEOG7WQ7VM.
TreeFamiTF336929.

Family and domain databases

InterProiIPR001588. Casein.
IPR016345. Casein_beta.
[Graphical view]
PANTHERiPTHR11500. PTHR11500. 1 hit.
PfamiPF00363. Casein. 1 hit.
[Graphical view]
PIRSFiPIRSF002372. Beta-casein. 1 hit.
PROSITEiPS00306. CASEIN_ALPHA_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02666-1 [UniParc]FASTAAdd to Basket

« Hide

MKVLILACLV ALALARELEE LNVPGEIVES LSSSEESITR INKKIEKFQS    50
EEQQQTEDEL QDKIHPFAQT QSLVYPFPGP IPNSLPQNIP PLTQTPVVVP 100
PFLQPEVMGV SKVKEAMAPK HKEMPFPKYP VEPFTESQSL TLTDVENLHL 150
PLPLLQSWMH QPHQPLPPTV MFPPQSVLSL SQSKVLPVPQ KAVPYPQRDM 200
PIQAFLLYQE PVLGPVRGPF PIIV 224
Length:224
Mass (Da):25,107
Last modified:July 1, 1989 - v2
Checksum:iF0BBDD8148A238AE
GO

Sequence cautioni

The sequence ABL74247.1 differs from that shown. Reason: Frameshift at positions 65 and 71.
The sequence AAW84270.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAW84271.1 differs from that shown. Reason: Erroneous initiation.

Mass spectrometryi

Molecular mass is 872.51 Da from positions 113 - 120. Determined by ESI. 1 Publication

Polymorphismi

Leu-152 is present in the variants F and G; Gln-190 and Glu-210 are present in the variant H. The sequence shown is the A2 variant.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti33 – 331S → K in variant D. 1 Publication
Natural varianti40 – 401R → C in variant H.
Natural varianti51 – 511E → K in variant E. 1 Publication
Natural varianti52 – 521E → K in variant C.
Natural varianti82 – 821P → H in variants A1, B, C, F and G. 3 Publications
Natural varianti103 – 1031L → I in variant H.
Natural varianti108 – 1081M → L.4 Publications
Natural varianti121 – 1211H → Q in variant A3. 2 Publications
Natural varianti132 – 1321E → Q in variants A1 and G. 2 Publications
Natural varianti137 – 1371S → R in variant B. 1 Publication
Natural varianti152 – 1521L → P in variants A1 and H. 1 Publication
Natural varianti153 – 1531P → L in variants A1, G and H. 1 Publication
Natural varianti167 – 1671P → L in variant F.
Natural varianti190 – 1901Q → E in variants A1 and G.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501S → Z AA sequence 1 Publication
Sequence conflicti69 – 691Q → R in ABL74247. 1 Publication
Sequence conflicti112 – 1121K → R in ABL74247. 1 Publication
Sequence conflicti208 – 2081Y → V in CAC37028. 1 Publication
Sequence conflicti209 – 2102QE → EQ AA sequence 1 Publication
Sequence conflicti210 – 2101E → Q in AAA30430. 1 Publication
Sequence conflicti210 – 2101E → Q no nucleotide entry 1 Publication
Sequence conflicti212 – 2121V → A in ABR10906. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M15132 mRNA. Translation: AAA30430.1.
X06359 mRNA. Translation: CAA29658.1.
M16645 mRNA. Translation: AAA30480.1.
M55158 Genomic DNA. Translation: AAA30431.1.
S67277 mRNA. Translation: AAB29137.1.
BC111172 mRNA. Translation: AAI11173.1.
AY899917 mRNA. Translation: AAW84270.1. Different initiation.
AY899918 mRNA. Translation: AAW84271.1. Different initiation.
AH007287 Genomic DNA. Translation: AAD09813.1.
EF123100 Genomic DNA. Translation: ABL74247.1. Frameshift.
EF628290 Genomic DNA. Translation: ABR10906.1.
AJ296330 Genomic DNA. Translation: CAC37028.1.
M64756 mRNA. Translation: AAB59254.1.
AY366419 Genomic DNA. Translation: AAR14677.1.
K01087 mRNA. Translation: AAA30481.1.
PIRiA59068.
I45873. KBBOA2.
UniGeneiBt.53272.

Genome annotation databases

EnsembliENSBTAT00000003409; ENSBTAP00000003409; ENSBTAG00000002632.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Protein Spotlight

Of buttons, digestion and glue - Issue 16 of November 2001

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M15132 mRNA. Translation: AAA30430.1 .
X06359 mRNA. Translation: CAA29658.1 .
M16645 mRNA. Translation: AAA30480.1 .
M55158 Genomic DNA. Translation: AAA30431.1 .
S67277 mRNA. Translation: AAB29137.1 .
BC111172 mRNA. Translation: AAI11173.1 .
AY899917 mRNA. Translation: AAW84270.1 . Different initiation.
AY899918 mRNA. Translation: AAW84271.1 . Different initiation.
AH007287 Genomic DNA. Translation: AAD09813.1 .
EF123100 Genomic DNA. Translation: ABL74247.1 . Frameshift.
EF628290 Genomic DNA. Translation: ABR10906.1 .
AJ296330 Genomic DNA. Translation: CAC37028.1 .
M64756 mRNA. Translation: AAB59254.1 .
AY366419 Genomic DNA. Translation: AAR14677.1 .
K01087 mRNA. Translation: AAA30481.1 .
PIRi A59068.
I45873. KBBOA2.
UniGenei Bt.53272.

3D structure databases

DisProti DP00329.
ProteinModelPortali P02666.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 158462. 1 interaction.
DIPi DIP-46257N.
IntActi P02666. 2 interactions.
STRINGi 9913.ENSBTAP00000003409.

Protein family/group databases

Allergomei 10199. Bos d 11.0101.
167. Bos d 8.
2736. Bos d 11.

Proteomic databases

PaxDbi P02666.
PRIDEi P02666.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000003409 ; ENSBTAP00000003409 ; ENSBTAG00000002632 .

Phylogenomic databases

eggNOGi NOG45871.
GeneTreei ENSGT00390000001890.
HOVERGENi HBG004973.
InParanoidi P02666.
OMAi EIMEVPK.
OrthoDBi EOG7WQ7VM.
TreeFami TF336929.

Enzyme and pathway databases

Reactomei REACT_220588. Nuclear signaling by ERBB4.

Miscellaneous databases

NextBioi 20805172.
PMAP-CutDB P02666.

Family and domain databases

InterProi IPR001588. Casein.
IPR016345. Casein_beta.
[Graphical view ]
PANTHERi PTHR11500. PTHR11500. 1 hit.
Pfami PF00363. Casein. 1 hit.
[Graphical view ]
PIRSFi PIRSF002372. Beta-casein. 1 hit.
PROSITEi PS00306. CASEIN_ALPHA_BETA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS LEU-108; PRO-152 AND LEU-153.
  2. "Primary structure of bovine beta-casein cDNA."
    Baev A.A., Smirnov I.K., Gorodetsky S.I.
    Mol. Biol. (Mosk.) 21:214-222(1987)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT HIS-82.
  3. "Complete nucleotide sequences of bovine alpha S2- and beta-casein cDNAs: comparisons with related sequences in other species."
    Stewart A.F., Bonsing J., Beattie C.W., Shah F., Willis I.M., Mackinlay A.G.
    Mol. Biol. Evol. 4:231-241(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Complete nucleotide sequence of the bovine beta-casein gene."
    Bonsing J., Ring J.M., Stewart A.F., Mackinlay A.G.
    Aust. J. Biol. Sci. 41:527-537(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT HIS-82.
  5. "Overproduction of bovine beta-casein in Escherichia coli and engineering of its main chymosin cleavage site."
    Simons G., van den Heuvel W., Reynen T., Frijters A., Rutten G., Slangen C.J., Groenen M., de Vos W.M., Siezen R.J.
    Protein Eng. 6:763-770(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT A3 GLN-121.
    Tissue: Mammary gland.
  6. NIH - Mammalian Gene Collection (MGC) project
    Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS HIS-82 AND ARG-137.
    Strain: Crossbred X Angus.
    Tissue: Liver.
  7. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-101.
    Tissue: Mammary epithelium.
  8. "Primary structure of bovine beta casein. Complete sequence."
    Ribadeau-Dumas B., Brignon G., Grosclaude F., Mercier J.-C.
    Eur. J. Biochem. 25:505-514(1972) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 16-224 (VARIANT A2), VARIANT LEU-108.
  9. "A new strategy for primary structure determination of proteins: application to bovine beta-casein."
    Carles C., Huet J.-C., Ribadeau-Dumas B.
    FEBS Lett. 229:265-272(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 16-224 (VARIANT A2).
  10. "Biochemical, molecular and physiological characterization of a new beta-casein variant detected in Korean cattle."
    Han S.K., Shin Y.C., Byun H.D.
    Anim. Genet. 31:49-51(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 18-57, PROTEIN SEQUENCE OF 16-224 (VARIANT H), VARIANT D LYS-33.
    Strain: Korean.
    Tissue: Milk.
  11. "Analysis of bovine beta-casein tryptic digest by continuous-flow fast-atom bombardment mass spectrometry."
    Jones D.S., Heerma W., van Wassenaar P.D., Haverkamp J.
    Rapid Commun. Mass Spectrom. 5:192-195(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 41-71; 113-157 AND 180-224, VARIANT GLN-132.
  12. "Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties."
    Lebrun I., Cavallaro V., Juliano L., Juliano M.A., de Sousa e Silva M.C.C.
    Mediators Inflamm. 13:263-268(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 41-45, FUNCTION.
  13. "The beta E variant and the phosphorylation code of bovine caseins."
    Grosclaude F., Mahe M.-F., Voglino G.-F.
    FEBS Lett. 45:3-5(1974) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 48-63, VARIANT E LYS-51.
  14. "Polymorphisms in beta and kappa casein genes in bubaline and bovine."
    Otaviano A.R., Lima A.L.F., Laureano M.M.M., Albuquerque L.G., Tonhati H., Sena J.A.D.
    Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 58-223.
  15. "Polymorphism in the cattle beta casein gene."
    Shahla M.N., Cheema F.R., Naeem M.K., Riazuddin S.
    Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 58-223.
  16. "Characterization of milk proteins."
    Klotz A., Buchberger J., Krause I., Einspanier R.
    Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 63-208.
    Tissue: Mammary gland.
  17. "Identification of bacterial clones encoding bovine caseins by direct immunological screening of the cDNA library."
    Ivanov V.N., Kershulite D.R., Bayev A.A., Akhundova A.A., Sulimova G.E., Judinkova E.S., Gorodetsky S.I.
    Gene 32:381-388(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 68-105.
  18. "Identification of bacterial clones that encode cow's caseins by direct immunological screening of the cDNA library."
    Ivanov V.N., Kershulite D.R., Bayev A.A., Akhundova A.A., Silimova G.E.
    Mol. Biol. (Mosk.) 19:955-963(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 68-95.
  19. "Peptic digestion of beta-casein: Time course and fate of possible bioactive peptides."
    Schmelzer C.E.H., Schoeps R., Reynell L., Ulbrich-Hofmann R., Neubert R.H.H., Raith K.
    J. Chromatogr. A 1166:108-115(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 74-108, VARIANT LEU-108, PHOSPHORYLATION, IDENTIFICATION BY MASS SPECTROMETRY.
  20. "A new variant in exon VII of bovine beta-casein gene (CSN2) and its contribution among European cattle breeds."
    Jann O., Ceriotti G., Caroli A., Erhardt G.
    J. Anim. Breed. Genet. 119:65-68(2002)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 80-143, VARIANT LEU-108.
  21. "Studies on antioxidative peptides generated in cheddar cheese."
    Gupta A., Mann B., Kumar Bajaj R., Sangwan R.B.
    Submitted (JAN-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 113-120, FUNCTION, MASS SPECTROMETRY.
  22. "Localization in the peptide chain of bovine beta casein of the His-Gln substitution differentiating the A2 and A3 genetic variants."
    Ribadeau-Dumas B., Grosclaude F., Mercier J.-C.
    C. R. Hebd. Seances Acad. Sci., D, Sci. Nat. 270:2369-2372(1970) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 118-124, VARIANT A3 GLN-121.
  23. "Characterization of a non-electrophoretic genetic variant of beta-casein by peptide mapping and mass spectrometric analysis."
    Dong C., Ng-Kwai-Hang K.F.
    Int. Dairy J. 8:967-972(1998)
    [AGRICOLA] [Europe PMC]
    Cited for: PROTEIN SEQUENCE OF 125-195 (VARIANTS A1 AND G).
  24. "Isolation and characterization of a new bradykinin potentiating octapeptide from gamma-casein."
    Lebrun I., Lebrun F.L.A.S., Henriques O.B., Carmona A.K., Juliano L., Camargo A.C.M.
    Can. J. Physiol. Pharmacol. 73:85-91(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 129-136, FUNCTION, VARIANT GLN-132.
  25. "Biochemical and pharmacological aspects of two bradykinin-potentiating peptides obtained from tryptic hydrolysis of casein."
    Perpetuo E.A., Juliano L., Lebrun I.
    J. Protein Chem. 22:601-606(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 129-136, FUNCTION.
  26. "Identification of a new genetic variant of bovine beta-casein using reversed-phase high-performance liquid chromatography and mass spectrometric analysis."
    Visser S., Slangen C.J., Lagerwerf F.M., Van Dongen W.D., Haverkamp J.
    J. Chromatogr. A 711:141-150(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 160-171 (VARIANT F).
  27. "Construction and identification by partial nucleotide sequence analysis of bovine casein and beta-lactoglobulin cDNA clones."
    Willis I.M., Stewart A.F., Caputo A., Thompson A.R., McKinlay A.G.
    DNA 1:375-386(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 170-184.
  28. "Extended Range Proteomic Analysis (ERPA): a new and sensitive LC-MS platform for high sequence coverage of complex proteins with extensive post-translational modifications-comprehensive analysis of beta-casein and epidermal growth factor receptor (EGFR)."
    Wu S.L., Kim J., Hancock W.S., Karger B.
    J. Proteome Res. 4:1155-1170(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-30; SER-32; SER-33; SER-34 AND SER-50, IDENTIFICATION BY MASS SPECTROMETRY.
  29. "Reference-facilitated phosphoproteomics: fast and reliable phosphopeptide validation by micro LC-ESI-Q-TOF MS/MS."
    Imanishi S.Y., Kochin V., Ferraris S.E., de Thonel A., Pallari H.M., Corthals G.L., Eriksson J.E.
    Mol. Cell. Proteomics 6:1380-1391(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-30; SER-32; SER-33; SER-34 AND SER-50, IDENTIFICATION BY MASS SPECTROMETRY.
  30. "Characterization of genetic variants of alpha-S1 and beta bovine caseins."
    Grosclaude F., Mahe M.-F., Mercier J.-C., Ribadeau-Dumas B.
    Eur. J. Biochem. 26:328-337(1972) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS A1; B AND C.

Entry informationi

Entry nameiCASB_BOVIN
AccessioniPrimary (citable) accession number: P02666
Secondary accession number(s): A1YQZ8
, A6N8V0, Q2TA13, Q5EEQ6, Q5EEQ7, Q6UN63, Q9BDG5, Q9TSD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1989
Last modified: September 3, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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