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P02663 (CASA2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-S2-casein

Cleaved into the following chain:

  1. Casocidin-1
    Alternative name(s):
    Casocidin-I
Gene names
Name:CSN1S2
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length222 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Important role in the capacity of milk to transport calcium phosphate.

Casocidin-I inhibits the growth of E.coli and S.carnosus.

Subcellular location

Secreted.

Tissue specificity

Mammary gland specific. Secreted in milk.

Polymorphism

At least two alleles exist. The sequence of the A allele is shown here. The D allele sequence differs from that shown in having a deletion of nine residues, which may be 49-58, 50-59, or 51-60.

Sequence similarities

Belongs to the alpha-casein family.

Mass spectrometry

Molecular mass is 4870 Da from positions 165 - 203. Determined by ESI. Ref.5

Ontologies

Keywords
   Cellular componentSecreted
   DomainRepeat
Signal
   Molecular functionAntibiotic
Antimicrobial
Milk protein
   PTMPhosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processdefense response to bacterium

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontransporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1515 Ref.3
Chain16 – 222207Alpha-S2-casein
PRO_0000004460
Peptide165 – 20339Casocidin-1 Ref.5
PRO_0000004461

Regions

Repeat76 – 14065
Repeat158 – 22265

Amino acid modifications

Modified residue231Phosphoserine Ref.7
Modified residue241Phosphoserine Ref.7
Modified residue251Phosphoserine Ref.7
Modified residue281Phosphoserine Ref.7
Modified residue461Phosphoserine Ref.6
Modified residue711Phosphoserine Ref.7
Modified residue721Phosphoserine Ref.7
Modified residue731Phosphoserine Ref.7
Modified residue761Phosphoserine Ref.7
Modified residue1441Phosphoserine
Modified residue1461Phosphoserine
Modified residue1581Phosphoserine Ref.6 Ref.7

Experimental info

Sequence conflict421A → D in AAI14774. Ref.2
Sequence conflict1021Q → E AA sequence Ref.3
Sequence conflict1051Q → R in AAI14774. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P02663 [UniParc].

Last modified July 1, 1989. Version 2.
Checksum: 81E7408AF1C12F7C

FASTA22226,019
        10         20         30         40         50         60 
MKFFIFTCLL AVALAKNTME HVSSSEESII SQETYKQEKN MAINPSKENL CSTFCKEVVR 

        70         80         90        100        110        120 
NANEEEYSIG SSSEESAEVA TEEVKITVDD KHYQKALNEI NQFYQKFPQY LQYLYQGPIV 

       130        140        150        160        170        180 
LNPWDQVKRN AVPITPTLNR EQLSTSEENS KKTVDMESTE VFTKKTKLTE EEKNRLNFLK 

       190        200        210        220 
KISQRYQKFA LPQYLKTVYQ HQKAMKPWIQ PKTKVIPYVR YL 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequences of bovine alpha S2- and beta-casein cDNAs: comparisons with related sequences in other species."
Stewart A.F., Bonsing J., Beattie C.W., Shah F., Willis I.M., Mackinlay A.G.
Mol. Biol. Evol. 4:231-241(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Liver.
[3]"Complete amino acid sequence of bovine alphaS2-casein."
Brignon G., Ribadeau-Dumas B., Mercier J.-C., Pelissier J.-P., Das B.C.
FEBS Lett. 76:274-279(1977) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 16-222 (A ALLELE).
Tissue: Milk.
[4]"A genetic and biochemical analysis of a polymorphism of bovine alpha S2-casein."
Grosclaude F., Joudrier P., Mahe M.-F.
J. Dairy Res. 46:211-213(1979) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE (D ALLELE).
Tissue: Milk.
[5]"Casocidin-I: a casein-alpha s2 derived peptide exhibits antibacterial activity."
Zucht H.-D., Raida M., Adermann K., Meagert H.-J., Forssmann W.-G.
FEBS Lett. 372:185-188(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 165-203, CHARACTERIZATION OF CASOCIDIN, MASS SPECTROMETRY.
Tissue: Milk.
[6]Bai F., Liu S., Witzmann F.A.
Submitted (SEP-2005) to UniProtKB
Cited for: PHOSPHORYLATION AT SER-46 AND SER-158.
[7]"Reference-facilitated phosphoproteomics: fast and reliable phosphopeptide validation by micro LC-ESI-Q-TOF MS/MS."
Imanishi S.Y., Kochin V., Ferraris S.E., de Thonel A., Pallari H.M., Corthals G.L., Eriksson J.E.
Mol. Cell. Proteomics 6:1380-1391(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-23; SER-24; SER-25; SER-28; SER-71; SER-72; SER-73; SER-76 AND SER-158, IDENTIFICATION BY MASS SPECTROMETRY.

Web resources

Protein Spotlight

Of buttons, digestion and glue - Issue 16 of November 2001

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M16644 mRNA. Translation: AAA30479.1.
BC114773 mRNA. Translation: AAI14774.1.
PIRKABOS2. JQ2008.
RefSeqNP_776953.1. NM_174528.2.
UniGeneBt.57616.

3D structure databases

ProteinModelPortalP02663.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

Allergome10198. Bos d 10.0101.
167. Bos d 8.
2735. Bos d 10.

Proteomic databases

PaxDbP02663.
PRIDEP02663.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000006590; ENSBTAP00000006590; ENSBTAG00000005005.
GeneID282209.
KEGGbta:282209.

Organism-specific databases

CTD100327035.

Phylogenomic databases

eggNOGNOG128783.
GeneTreeENSGT00530000065240.
HOGENOMHOG000111307.
HOVERGENHBG005244.
InParanoidP02663.
OMAVMNPWDQ.
OrthoDBEOG7MWH0G.
TreeFamTF339561.

Family and domain databases

InterProIPR011175. Alpha-s2_casein.
IPR001588. Casein.
[Graphical view]
PANTHERPTHR16656:SF2. PTHR16656:SF2. 1 hit.
PfamPF00363. Casein. 2 hits.
[Graphical view]
PIRSFPIRSF002371. Alpha-s2-casein. 1 hit.
PROSITEPS00306. CASEIN_ALPHA_BETA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20806034.
PMAP-CutDBP02663.

Entry information

Entry nameCASA2_BOVIN
AccessionPrimary (citable) accession number: P02663
Secondary accession number(s): Q1RMQ6, Q9TR51
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries