Reviewed, UniProtKB/Swiss-Prot P02663 (CASA2_BOVIN)

Names and origin

Protein names

Recommended name:
    Alpha-S2-casein
Cleaved into the following chain:
    1- Recommended name:
            Casocidin-1
        Alternative name(s):
            Casocidin-I

Gene names

Name:

CSN1S2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Important role in the capacity of milk to transport calcium phosphate. Casocidin-I inhibits the growth of E.coli and S.carnosus.
Subcellular location	Secreted.
Tissue specificity	Mammary gland specific. Secreted in milk.
Polymorphism	At least two alleles exist. The sequence of the A allele is shown here. The D allele sequence differs from that shown in having a deletion of nine residues, which may be 49-58, 50-59, or 51-60.
Sequence similarities	Belongs to the alpha-casein family.
Mass spectrometry	Molecular mass is 4870 Da from positions 165 - 203. Determined by ESI. Ref.5

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Antibiotic Antimicrobial Milk protein
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Ref.3
	Chain	16 – 222	207	Alpha-S2-casein		PRO_0000004460
	Peptide	165 – 203	39	Casocidin-1		PRO_0000004461
Regions
	Repeat	76 – 140	65
	Repeat	158 – 222	65
Amino acid modifications
	Modified residue	23	1	Phosphoserine Ref.7
	Modified residue	24	1	Phosphoserine Ref.7
	Modified residue	25	1	Phosphoserine Ref.7
	Modified residue	31	1	Phosphoserine
	Modified residue	46	1	Phosphoserine Ref.6
	Modified residue	71	1	Phosphoserine Ref.7
	Modified residue	72	1	Phosphoserine Ref.7
	Modified residue	73	1	Phosphoserine Ref.7
	Modified residue	76	1	Phosphoserine Ref.7
	Modified residue	144	1	Phosphoserine
	Modified residue	146	1	Phosphoserine
	Modified residue	158	1	Phosphoserine Ref.7 Ref.6
Experimental info
	Sequence conflict	42	1	A → D in AAI14774. Ref.2
	Sequence conflict	102	1	Q → E AA sequence Ref.3
	Sequence conflict	105	1	Q → R in AAI14774. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P02663-1 [UniParc]. Last modified July 1, 1989. Version 2. Checksum: 81E7408AF1C12F7C	FASTA	222	26,019
	10 20 30 40 50 60 MKFFIFTCLL AVALAKNTME HVSSSEESII SQETYKQEKN MAINPSKENL CSTFCKEVVR 70 80 90 100 110 120 NANEEEYSIG SSSEESAEVA TEEVKITVDD KHYQKALNEI NQFYQKFPQY LQYLYQGPIV 130 140 150 160 170 180 LNPWDQVKRN AVPITPTLNR EQLSTSEENS KKTVDMESTE VFTKKTKLTE EEKNRLNFLK 190 200 210 220 KISQRYQKFA LPQYLKTVYQ HQKAMKPWIQ PKTKVIPYVR YL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequences of bovine alpha S2- and beta-casein cDNAs: comparisons with related sequences in other species." Stewart A.F., Bonsing J., Beattie C.W., Shah F., Willis I.M., Mackinlay A.G. Mol. Biol. Evol. 4:231-241(1987) [PubMed: 2833669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.
[3]	"Complete amino acid sequence of bovine alphaS2-casein." Brignon G., Ribadeau-Dumas B., Mercier J.-C., Pelissier J.-P., Das B.C. FEBS Lett. 76:274-279(1977) [PubMed: 862906] [Abstract] Cited for: PROTEIN SEQUENCE OF 16-222 (A ALLELE). Tissue: Milk.
[4]	"A genetic and biochemical analysis of a polymorphism of bovine alpha S2-casein." Grosclaude F., Joudrier P., Mahe M.-F. J. Dairy Res. 46:211-213(1979) [PubMed: 469044] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE (D ALLELE). Tissue: Milk.
[5]	"Casocidin-I: a casein-alpha s2 derived peptide exhibits antibacterial activity." Zucht H.-D., Raida M., Adermann K., Meagert H.-J., Forssmann W.-G. FEBS Lett. 372:185-188(1995) [PubMed: 7556666] [Abstract] Cited for: PROTEIN SEQUENCE OF 165-203, CHARACTERIZATION OF CASOCIDIN, MASS SPECTROMETRY. Tissue: Milk.
[6]	Bai F., Liu S., Witzmann F.A. Submitted (SEP-2005) to UniProtKB Cited for: PHOSPHORYLATION AT SER-46 AND SER-158.
[7]	"Reference-facilitated phosphoproteomics: fast and reliable phosphopeptide validation by micro LC-ESI-Q-TOF MS/MS." Imanishi S.Y., Kochin V., Ferraris S.E., de Thonel A., Pallari H.M., Corthals G.L., Eriksson J.E. Mol. Cell. Proteomics 6:1380-1391(2007) [PubMed: 17510049] [Abstract] Cited for: PHOSPHORYLATION AT SER-23; SER-24; SER-25; SER-28; SER-71; SER-72; SER-73; SER-76 AND SER-158, MASS SPECTROMETRY.

Web resources

Protein Spotlight

Of buttons, digestion and glue - Issue 16 of November 2001

Cross-references

Sequence databases
EMBLGenBankDDBJ	M16644 mRNA. Translation: AAA30479.1. BC114773 mRNA. Translation: AAI14774.1.
IPI	IPI00698843.
PIR	KABOS2. JQ2008.
RefSeq	NP_776953.1.
UniGene	Bt.57616
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000005005. Bos taurus. [Contig view]
GeneID	282209.
KEGG	bta:282209.
Phylogenomic databases
HOVERGEN	P02663.
OMA	P02663. SEETICA.
Family and domain databases
InterPro	IPR001588. Casein. IPR011175. Casein_alpha-s2. [Graphical view]
PANTHER	PTHR16656:SF2. Alpha-s2-casein. 1 hit.
Pfam	PF00363. Casein. 2 hits. [Graphical view]
PIRSF	PIRSF002371. Alpha-s2-casein. 1 hit.
PROSITE	PS00306. CASEIN_ALPHA_BETA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
PMAP-CutDB	P02663.

Entry information

Entry name

CASA2_BOVIN

Accession

Primary (citable) accession number: P02663
Secondary accession number(s): Q1RMQ6, Q9TR51

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 1, 1989
Last modified:	June 16, 2009
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)