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Protein

Alpha-S2-casein

Gene

CSN1S2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Important role in the capacity of milk to transport calcium phosphate.
Casocidin-I inhibits the growth of E.coli and S.carnosus.

GO - Molecular functioni

  1. transporter activity Source: InterPro

GO - Biological processi

  1. defense response to bacterium Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Milk protein

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-S2-casein
Cleaved into the following chain:
Alternative name(s):
Casocidin-I
Gene namesi
Name:CSN1S2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 6

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei10198. Bos d 10.0101.
167. Bos d 8.
2735. Bos d 10.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 15151 PublicationAdd
BLAST
Chaini16 – 222207Alpha-S2-caseinPRO_0000004460Add
BLAST
Peptidei165 – 20339Casocidin-1PRO_0000004461Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei23 – 231Phosphoserine1 Publication
Modified residuei24 – 241Phosphoserine1 Publication
Modified residuei25 – 251Phosphoserine1 Publication
Modified residuei28 – 281Phosphoserine1 Publication
Modified residuei46 – 461Phosphoserine1 Publication
Modified residuei71 – 711Phosphoserine1 Publication
Modified residuei72 – 721Phosphoserine1 Publication
Modified residuei73 – 731Phosphoserine1 Publication
Modified residuei76 – 761Phosphoserine1 Publication
Modified residuei144 – 1441Phosphoserine
Modified residuei146 – 1461Phosphoserine
Modified residuei158 – 1581Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP02663.
PRIDEiP02663.

Miscellaneous databases

PMAP-CutDBP02663.

Expressioni

Tissue specificityi

Mammary gland specific. Secreted in milk.

Structurei

3D structure databases

ProteinModelPortaliP02663.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati76 – 14065Add
BLAST
Repeati158 – 22265Add
BLAST

Sequence similaritiesi

Belongs to the alpha-casein family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG128783.
GeneTreeiENSGT00530000065240.
HOGENOMiHOG000111307.
HOVERGENiHBG005244.
InParanoidiP02663.
OMAiVMNPWDQ.
OrthoDBiEOG7MWH0G.
TreeFamiTF339561.

Family and domain databases

InterProiIPR011175. Alpha-s2_casein.
IPR001588. Casein.
[Graphical view]
PfamiPF00363. Casein. 2 hits.
[Graphical view]
PIRSFiPIRSF002371. Alpha-s2-casein. 1 hit.
PROSITEiPS00306. CASEIN_ALPHA_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02663-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFFIFTCLL AVALAKNTME HVSSSEESII SQETYKQEKN MAINPSKENL
60 70 80 90 100
CSTFCKEVVR NANEEEYSIG SSSEESAEVA TEEVKITVDD KHYQKALNEI
110 120 130 140 150
NQFYQKFPQY LQYLYQGPIV LNPWDQVKRN AVPITPTLNR EQLSTSEENS
160 170 180 190 200
KKTVDMESTE VFTKKTKLTE EEKNRLNFLK KISQRYQKFA LPQYLKTVYQ
210 220
HQKAMKPWIQ PKTKVIPYVR YL
Length:222
Mass (Da):26,019
Last modified:July 1, 1989 - v2
Checksum:i81E7408AF1C12F7C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti42 – 421A → D in AAI14774 (Ref. 2) Curated
Sequence conflicti102 – 1021Q → E AA sequence (PubMed:862906).Curated
Sequence conflicti105 – 1051Q → R in AAI14774 (Ref. 2) Curated

Mass spectrometryi

Molecular mass is 4870 Da from positions 165 - 203. Determined by ESI. 1 Publication

Polymorphismi

At least two alleles exist. The sequence of the A allele is shown here. The D allele sequence differs from that shown in having a deletion of nine residues, which may be 49-58, 50-59, or 51-60.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16644 mRNA. Translation: AAA30479.1.
BC114773 mRNA. Translation: AAI14774.1.
PIRiJQ2008. KABOS2.
RefSeqiNP_776953.1. NM_174528.2.
UniGeneiBt.57616.

Genome annotation databases

EnsembliENSBTAT00000006590; ENSBTAP00000006590; ENSBTAG00000005005.
GeneIDi282209.
KEGGibta:282209.

Cross-referencesi

Web resourcesi

Protein Spotlight

Of buttons, digestion and glue - Issue 16 of November 2001

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16644 mRNA. Translation: AAA30479.1.
BC114773 mRNA. Translation: AAI14774.1.
PIRiJQ2008. KABOS2.
RefSeqiNP_776953.1. NM_174528.2.
UniGeneiBt.57616.

3D structure databases

ProteinModelPortaliP02663.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei10198. Bos d 10.0101.
167. Bos d 8.
2735. Bos d 10.

Proteomic databases

PaxDbiP02663.
PRIDEiP02663.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000006590; ENSBTAP00000006590; ENSBTAG00000005005.
GeneIDi282209.
KEGGibta:282209.

Organism-specific databases

CTDi100327035.

Phylogenomic databases

eggNOGiNOG128783.
GeneTreeiENSGT00530000065240.
HOGENOMiHOG000111307.
HOVERGENiHBG005244.
InParanoidiP02663.
OMAiVMNPWDQ.
OrthoDBiEOG7MWH0G.
TreeFamiTF339561.

Miscellaneous databases

NextBioi20806034.
PMAP-CutDBP02663.

Family and domain databases

InterProiIPR011175. Alpha-s2_casein.
IPR001588. Casein.
[Graphical view]
PfamiPF00363. Casein. 2 hits.
[Graphical view]
PIRSFiPIRSF002371. Alpha-s2-casein. 1 hit.
PROSITEiPS00306. CASEIN_ALPHA_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequences of bovine alpha S2- and beta-casein cDNAs: comparisons with related sequences in other species."
    Stewart A.F., Bonsing J., Beattie C.W., Shah F., Willis I.M., Mackinlay A.G.
    Mol. Biol. Evol. 4:231-241(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.
  3. Cited for: PROTEIN SEQUENCE OF 16-222 (A ALLELE).
    Tissue: Milk.
  4. "A genetic and biochemical analysis of a polymorphism of bovine alpha S2-casein."
    Grosclaude F., Joudrier P., Mahe M.-F.
    J. Dairy Res. 46:211-213(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE (D ALLELE).
    Tissue: Milk.
  5. "Casocidin-I: a casein-alpha s2 derived peptide exhibits antibacterial activity."
    Zucht H.-D., Raida M., Adermann K., Meagert H.-J., Forssmann W.-G.
    FEBS Lett. 372:185-188(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 165-203, CHARACTERIZATION OF CASOCIDIN, MASS SPECTROMETRY.
    Tissue: Milk.
  6. Bai F., Liu S., Witzmann F.A.
    Submitted (SEP-2005) to UniProtKB
    Cited for: PHOSPHORYLATION AT SER-46 AND SER-158.
  7. "Reference-facilitated phosphoproteomics: fast and reliable phosphopeptide validation by micro LC-ESI-Q-TOF MS/MS."
    Imanishi S.Y., Kochin V., Ferraris S.E., de Thonel A., Pallari H.M., Corthals G.L., Eriksson J.E.
    Mol. Cell. Proteomics 6:1380-1391(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-23; SER-24; SER-25; SER-28; SER-71; SER-72; SER-73; SER-76 AND SER-158, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiCASA2_BOVIN
AccessioniPrimary (citable) accession number: P02663
Secondary accession number(s): Q1RMQ6, Q9TR51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1989
Last modified: January 7, 2015
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.