P02662 (CASA1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-S1-casein

Alternative name(s):
Allergen=Bos d 8

Cleaved into the following chain:

1. Antioxidant peptide

Gene names

Name:

CSN1S1

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Important role in the capacity of milk to transport calcium phosphate. Ref.15 Antioxidant peptide has 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Ref.15
Subcellular location	Secreted.
Tissue specificity	Mammary gland specific. Secreted in milk.
Allergenic properties	Causes an allergic reaction in human. Has six major and 3 minor IgE-binding regions and 5 major and 1 minor IgG-binding regions. Is a cause of cow's milk allergy (CMA). Ref.5 Ref.21
Miscellaneous	The B variant sequence is shown.
Sequence similarities	Belongs to the alpha-casein family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Disease	Allergen
Domain	Repeat Signal
Molecular function	Antioxidant Milk protein
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	antioxidant activity Inferred from electronic annotation. Source: UniProtKB-KW transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Ref.7 Ref.9
	Chain	16 – 214	199	Alpha-S1-casein		PRO_0000004446
	Peptide	95 – 105	11	Antioxidant peptide Ref.15		PRO_0000331578
Regions
	Repeat	85 – 99	15
	Repeat	125 – 140	16
Amino acid modifications
	Modified residue	56	1	Phosphoserine Ref.22 Ref.23
	Modified residue	61	1	Phosphoserine Ref.22
	Modified residue	63	1	Phosphoserine Ref.22
	Modified residue	79	1	Phosphoserine Ref.22
	Modified residue	81	1	Phosphoserine Ref.22
	Modified residue	82	1	Phosphoserine Ref.22
	Modified residue	83	1	Phosphoserine Ref.22
	Modified residue	90	1	Phosphoserine Ref.22
	Modified residue	130	1	Phosphoserine Ref.22
Natural variations
	Natural variant	29 – 41	13	Missing in variant A.
	Natural variant	68	1	A → T in variant D.
	Natural variant	207	1	E → G in variant C.
Experimental info
	Sequence conflict	11 – 12	2	AV → SA in ABW98943. Ref.5
	Sequence conflict	42	1	P → L in AAA30429. Ref.3
	Sequence conflict	44	1	P → S in ABW98945. Ref.5
	Sequence conflict	95	1	H → Q in AAA30478. Ref.13
	Sequence conflict	99	1	E → D in ABQ88318. Ref.14
	Sequence conflict	143	1	H → D in AAA30429. Ref.3
	Sequence conflict	171	1	L → P in ABW98953. Ref.5
	Sequence conflict	203	1	S → L in AAA62707. Ref.16
	Sequence conflict	211 – 212	2	MP → IS in AAA30429. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P02662 [UniParc]. Last modified November 1, 1990. Version 2. Checksum: F066B5C8AE55828B	FASTA	214	24,529
	10 20 30 40 50 60 MKLLILTCLV AVALARPKHP IKHQGLPQEV LNENLLRFFV APFPEVFGKE KVNELSKDIG 70 80 90 100 110 120 SESTEDQAME DIKQMEAESI SSSEEIVPNS VEQKHIQKED VPSERYLGYL EQLLRLKKYK 130 140 150 160 170 180 VPQLEIVPNS AEERLHSMKE GIHAQQKEPM IGVNQELAYF YPELFRQFYQ LDAYPSGAWY 190 200 210 YVPLGTQYTD APSFSDIPNP IGSENSEKTT MPLW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequences of bovine alpha S1- and kappa-casein cDNAs." Stewart A.F., Willis I.M., Mackinlay A.G. Nucleic Acids Res. 12:3895-3907(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation and sequence analysis of bovine alpha-S1-casein cDNA clone." Nagao M., Maki M., Sasaki R., Chiba R. Agric. Biol. Chem. 48:1663-1667(1984) Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"cDNA of cattle alpha S1-casein: cloning and nucleotide sequence." Gorodetskii S.I., Zakhar'ev V.M., Kyarshulite D.R., Kapelinskaya T.V., Skryabin K.G. Biokhimiia 51:1641-1648(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Mammary gland.
[4]	"Genomic organization of the bovine alpha-S1 casein gene." Koczan D., Hobom G., Seyfert H.-M. Nucleic Acids Res. 19:5591-5596(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Cloning, expression, and mapping of allergenic determinants of alphaS1-casein, a major cow's milk allergen." Schulmeister U., Hochwallner H., Swoboda I., Focke-Tejkl M., Geller B., Nystrand M., Harlin A., Thalhamer J., Scheiblhofer S., Keller W., Niggemann B., Quirce S., Ebner C., Mari A., Pauli G., Herz U., Valenta R., Spitzauer S. J. Immunol. 182:7019-7029(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALLERGEN.
[6]	NIH - Mammalian Gene Collection (MGC) project Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.
[7]	"Primary structure of bovine alpha-s1 casein. Complete sequence." Mercier J.-C., Grosclaude F., Ribadeau-Dumas B. Eur. J. Biochem. 23:41-51(1971) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 16-214 (VARIANT B).
[8]	"Primary structure of alpha casein and of bovine beta casein. Correction." Mercier J.-C., Grosclaude F., Ribadeau-Dumas B. Eur. J. Biochem. 40:323-323(1973) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO 74 AND 92-93 (VARIANTS A; B; C AND D).
[9]	"Characterization of genetic variants of alpha-S1 and beta bovine caseins." Grosclaude F., Mahe M.-F., Mercier J.-C., Ribadeau-Dumas B. Eur. J. Biochem. 26:328-337(1972) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 16-214 (VARIANT D).
[10]	"Localization in the N-terminal part of bovine casein alpha-s1 of a 13 amino-acid deletion that differentiates variant A from variants B and C." Grosclaude F., Mahe M.-F., Mercier J.-C., Ribadeau-Dumas B. FEBS Lett. 11:109-112(1970) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 23-49 (VARIANT A).
[11]	"Isolation of a new ligand-carrying casein fragment from bovine mammary gland microsomes." Neuteboom B., Giuffrida M.G., Conti A. FEBS Lett. 305:189-191(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 39-55. Tissue: Mammary gland.
[12]	"Action on bovine alpha s1-casein of cardosins A and B, aspartic proteinases from the flowers of the cardoon Cynara cardunculus L." Ramalho-Santos M., Verissimo P., Faro C., Pires E. Biochim. Biophys. Acta 1297:83-89(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 39-47 AND 166-188.
[13]	"Construction and identification by partial nucleotide sequence analysis of bovine casein and beta-lactoglobulin cDNA clones." Willis I.M., Stewart A.F., Caputo A., Thompson A.R., McKinlay A.G. DNA 1:375-386(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 55-130.
[14]	Shahla M.N., Farooq M.S., Naeem M.K., Riazuddin S. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 85-110.
[15]	"Antioxidant peptides isolated from cheddar cheese made with adjunct culture L.casei ssp. casei 300." Gupta A., Mann B., Kumar R., Sangwan R.B. Submitted (MAR-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 95-105, FUNCTION.
[16]	"Nucleotide sequence of the 3'-nontranslated region of the mRNA of alpha S1-casein in cows." Kiarshulite D.R., Zakhar'ev V.M., Gorodetskii S.I. Dokl. Akad. Nauk SSSR 280:1433-1437(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 122-214.
[17]	"Cloning, mapping, and sequencing of 3' and its flanking region of bovine alpha-s1 casein gene." Chen R., Wang B., Zhang Y., Liu W., Zhang J., Lao W. Chin. J. Biotechnol. 8:235-245(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 164-214.
[18]	"Cloning of cDNA sequence coding for bovine alpha-s1-casein." Maki M., Nagao M., Hirose M., Chiba H. Agric. Biol. Chem. 47:441-444(1983) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 185-202. Tissue: Mammary gland.
[19]	"On the localization in the C-terminal sequence of bovine casein alpha-s1 of a Glu/Gly substitution that differentiates the genetic variants B and C." Grosclaude F., Mercier J.-C., Ribadeau-Dumas B. C. R. Hebd. Seances Acad. Sci., D, Sci. Nat. 268:3133-3136(1970) Cited for: PROTEIN SEQUENCE OF 205-214 (VARIANT C).
[20]	Erratum Grosclaude F., Mercier J.-C., Ribadeau-Dumas B. C. R. Hebd. Seances Acad. Sci., D, Sci. Nat. 271:563-563(1970) Cited for: SEQUENCE REVISION.
[21]	"Identification of IgE- and IgG-binding epitopes on alpha(s1)-casein: differences in patients with persistent and transient cow's milk allergy." Chatchatee P., Jaervinen K.M., Bardina L., Beyer K., Sampson H.A. J. Allergy Clin. Immunol. 107:379-383(2001) [PubMed] [Europe PMC] [Abstract] Cited for: ALLERGEN.
[22]	"Highly selective enrichment of phosphorylated peptides from peptide mixtures using titanium dioxide microcolumns." Larsen M.R., Thingholm T.E., Jensen O.N., Roepstorff P., Jorgensen T.J. Mol. Cell. Proteomics 4:873-886(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-56; SER-61; SER-63; SER-79; SER-81; SER-82; SER-83; SER-90 AND SER-130, MASS SPECTROMETRY.
[23]	Bai F., Liu S., Witzmann F.A. Submitted (SEP-2005) to UniProtKB Cited for: PHOSPHORYLATION AT SER-56.

Web resources

Worthington enzyme manual

Protein Spotlight

Of buttons, digestion and glue - Issue 16 of November 2001

Cross-references

Sequence databases
EMBL GenBank DDBJ	X00564 mRNA. Translation: CAB57792.1. M33123 mRNA. Translation: AAA30428.1. M38641 mRNA. Translation: AAA30429.1. X59856 Genomic DNA. Translation: CAA42516.1. EU221551 Genomic DNA. Translation: ABW98936.1. EU221552 Genomic DNA. Translation: ABW98937.1. EU221553 Genomic DNA. Translation: ABW98938.1. EU221554 Genomic DNA. Translation: ABW98939.1. EU221555 Genomic DNA. Translation: ABW98940.1. EU221556 Genomic DNA. Translation: ABW98941.1. EU221557 Genomic DNA. Translation: ABW98942.1. EU221558 Genomic DNA. Translation: ABW98943.1. EU221559 Genomic DNA. Translation: ABW98944.1. EU221560 Genomic DNA. Translation: ABW98945.1. EU221561 Genomic DNA. Translation: ABW98946.1. EU221562 Genomic DNA. Translation: ABW98947.1. EU221563 Genomic DNA. Translation: ABW98948.1. EU221564 Genomic DNA. Translation: ABW98949.1. EU221565 Genomic DNA. Translation: ABW98950.1. EU221566 Genomic DNA. Translation: ABW98951.1. EU221567 Genomic DNA. Translation: ABW98952.1. EU221568 Genomic DNA. Translation: ABW98953.1. EU221569 Genomic DNA. Translation: ABW98954.1. EU221570 Genomic DNA. Translation: ABW98955.1. EU221571 Genomic DNA. Translation: ABW98956.1. EU221572 Genomic DNA. Translation: ABW98957.1. EU221573 Genomic DNA. Translation: ABW98958.1. EU221574 Genomic DNA. Translation: ABW98959.1. EU221575 Genomic DNA. Translation: ABW98960.1. EU221576 Genomic DNA. Translation: ABW98961.1. EU221577 Genomic DNA. Translation: ABW98962.1. EU221578 Genomic DNA. Translation: ABW98963.1. EU221579 Genomic DNA. Translation: ABW98964.1. EU221580 Genomic DNA. Translation: ABW98965.1. EU221581 Genomic DNA. Translation: ABW98966.1. BC109618 mRNA. Translation: AAI09619.1. K01084 mRNA. Translation: AAA30478.1. EF538766 Genomic DNA. Translation: ABQ88318.1. M38658 mRNA. Translation: AAA62707.1. AH007360 Genomic DNA. Translation: AAD14099.1. D00412 mRNA. Translation: BAA00313.1.
IPI	IPI00706094.
PIR	KABOSB. S22575. S72220.
RefSeq	NP_851372.1. NM_181029.2.
UniGene	Bt.59369.
3D structure databases
DisProt	DP00330.
ProteinModelPortal	P02662.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-29917N.
Protein family/group databases
Allergome	10197. Bos d 9.0101. 167. Bos d 8. 2734. Bos d 9.
Proteomic databases
PaxDb	P02662.
PRIDE	P02662.
ProMEX	P02662.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000010119; ENSBTAP00000010119; ENSBTAG00000007695.
GeneID	282208.
KEGG	bta:282208.
Organism-specific databases
CTD	1446.
Phylogenomic databases
eggNOG	NOG73245.
GeneTree	ENSGT00390000017378.
HOVERGEN	HBG005242.
InParanoid	P02662.
OrthoDB	EOG4M0F39.
Gene expression databases
ArrayExpress	P02662.
Family and domain databases
InterPro	IPR026999. Alpha-s1_casein. IPR001588. Casein. [Graphical view]
PANTHER	PTHR10240. PTHR10240. 1 hit.
Pfam	PF00363. Casein. 1 hit. [Graphical view]
PROSITE	PS00306. CASEIN_ALPHA_BETA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20806033.
PMAP-CutDB	P02662.

Entry information

Entry name

CASA1_BOVIN

Accession

Primary (citable) accession number: P02662
Secondary accession number(s): A5YK80 , A8WCP1, A8WCP2, A8WCP3, A8WCP4, A8WCP5, A8WCP6, A8WCP7, A8WCP8, A8WCP9, A8WCQ0, A8WCQ1, A8WCQ2, A8WCQ3, A8WCQ4, A8WCQ5, A8WCQ6, A8WCQ7, A8WCQ8, A8WCQ9, A8WCR0, A8WCR1, A8WCR2, A8WCR3, A8WCR4, A8WCR5, A8WCR6, A8WCR7, A8WCR8, A8WCR9, A8WCS0, A8WCS1, Q28048, Q28069, Q32LE8, Q7M2U6, Q9TRH5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	November 1, 1990
Last modified:	April 3, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program