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P02662 (CASA1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-S1-casein
Alternative name(s):
Allergen=Bos d 8

Cleaved into the following chain:

  1. Antioxidant peptide
Gene names
Name:CSN1S1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length214 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Important role in the capacity of milk to transport calcium phosphate. Ref.15

Antioxidant peptide has 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Ref.15

Subcellular location

Secreted.

Tissue specificity

Mammary gland specific. Secreted in milk.

Allergenic properties

Causes an allergic reaction in human. Has six major and 3 minor IgE-binding regions and 5 major and 1 minor IgG-binding regions. Is a cause of cow's milk allergy (CMA). Ref.5 Ref.21

Miscellaneous

The B variant sequence is shown.

Sequence similarities

Belongs to the alpha-casein family.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityPolymorphism
   DiseaseAllergen
   DomainRepeat
Signal
   Molecular functionAntioxidant
Milk protein
   PTMPhosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionantioxidant activity

Inferred from electronic annotation. Source: UniProtKB-KW

transporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1515 Ref.7 Ref.9
Chain16 – 214199Alpha-S1-casein
PRO_0000004446
Peptide95 – 10511Antioxidant peptide Ref.15
PRO_0000331578

Regions

Repeat85 – 9915
Repeat125 – 14016

Amino acid modifications

Modified residue561Phosphoserine Ref.22 Ref.23
Modified residue611Phosphoserine Ref.22
Modified residue631Phosphoserine Ref.22
Modified residue791Phosphoserine Ref.22
Modified residue811Phosphoserine Ref.22
Modified residue821Phosphoserine Ref.22
Modified residue831Phosphoserine Ref.22
Modified residue901Phosphoserine Ref.22
Modified residue1301Phosphoserine Ref.22

Natural variations

Natural variant29 – 4113Missing in variant A.
Natural variant681A → T in variant D.
Natural variant2071E → G in variant C.

Experimental info

Sequence conflict11 – 122AV → SA in ABW98943. Ref.5
Sequence conflict421P → L in AAA30429. Ref.3
Sequence conflict441P → S in ABW98945. Ref.5
Sequence conflict951H → Q in AAA30478. Ref.13
Sequence conflict991E → D in ABQ88318. Ref.14
Sequence conflict1431H → D in AAA30429. Ref.3
Sequence conflict1711L → P in ABW98953. Ref.5
Sequence conflict2031S → L in AAA62707. Ref.16
Sequence conflict211 – 2122MP → IS in AAA30429. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P02662 [UniParc].

Last modified November 1, 1990. Version 2.
Checksum: F066B5C8AE55828B

FASTA21424,529
        10         20         30         40         50         60 
MKLLILTCLV AVALARPKHP IKHQGLPQEV LNENLLRFFV APFPEVFGKE KVNELSKDIG 

        70         80         90        100        110        120 
SESTEDQAME DIKQMEAESI SSSEEIVPNS VEQKHIQKED VPSERYLGYL EQLLRLKKYK 

       130        140        150        160        170        180 
VPQLEIVPNS AEERLHSMKE GIHAQQKEPM IGVNQELAYF YPELFRQFYQ LDAYPSGAWY 

       190        200        210 
YVPLGTQYTD APSFSDIPNP IGSENSEKTT MPLW 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequences of bovine alpha S1- and kappa-casein cDNAs."
Stewart A.F., Willis I.M., Mackinlay A.G.
Nucleic Acids Res. 12:3895-3907(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Isolation and sequence analysis of bovine alpha-S1-casein cDNA clone."
Nagao M., Maki M., Sasaki R., Chiba R.
Agric. Biol. Chem. 48:1663-1667(1984)
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"cDNA of cattle alpha S1-casein: cloning and nucleotide sequence."
Gorodetskii S.I., Zakhar'ev V.M., Kyarshulite D.R., Kapelinskaya T.V., Skryabin K.G.
Biokhimiia 51:1641-1648(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Mammary gland.
[4]"Genomic organization of the bovine alpha-S1 casein gene."
Koczan D., Hobom G., Seyfert H.-M.
Nucleic Acids Res. 19:5591-5596(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Cloning, expression, and mapping of allergenic determinants of alphaS1-casein, a major cow's milk allergen."
Schulmeister U., Hochwallner H., Swoboda I., Focke-Tejkl M., Geller B., Nystrand M., Harlin A., Thalhamer J., Scheiblhofer S., Keller W., Niggemann B., Quirce S., Ebner C., Mari A., Pauli G., Herz U., Valenta R., Spitzauer S.
J. Immunol. 182:7019-7029(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALLERGEN.
[6]NIH - Mammalian Gene Collection (MGC) project
Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Liver.
[7]"Primary structure of bovine alpha-s1 casein. Complete sequence."
Mercier J.-C., Grosclaude F., Ribadeau-Dumas B.
Eur. J. Biochem. 23:41-51(1971) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 16-214 (VARIANT B).
[8]"Primary structure of alpha casein and of bovine beta casein. Correction."
Mercier J.-C., Grosclaude F., Ribadeau-Dumas B.
Eur. J. Biochem. 40:323-323(1973) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 74 AND 92-93 (VARIANTS A; B; C AND D).
[9]"Characterization of genetic variants of alpha-S1 and beta bovine caseins."
Grosclaude F., Mahe M.-F., Mercier J.-C., Ribadeau-Dumas B.
Eur. J. Biochem. 26:328-337(1972) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 16-214 (VARIANT D).
[10]"Localization in the N-terminal part of bovine casein alpha-s1 of a 13 amino-acid deletion that differentiates variant A from variants B and C."
Grosclaude F., Mahe M.-F., Mercier J.-C., Ribadeau-Dumas B.
FEBS Lett. 11:109-112(1970) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 23-49 (VARIANT A).
[11]"Isolation of a new ligand-carrying casein fragment from bovine mammary gland microsomes."
Neuteboom B., Giuffrida M.G., Conti A.
FEBS Lett. 305:189-191(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 39-55.
Tissue: Mammary gland.
[12]"Action on bovine alpha s1-casein of cardosins A and B, aspartic proteinases from the flowers of the cardoon Cynara cardunculus L."
Ramalho-Santos M., Verissimo P., Faro C., Pires E.
Biochim. Biophys. Acta 1297:83-89(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 39-47 AND 166-188.
[13]"Construction and identification by partial nucleotide sequence analysis of bovine casein and beta-lactoglobulin cDNA clones."
Willis I.M., Stewart A.F., Caputo A., Thompson A.R., McKinlay A.G.
DNA 1:375-386(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 55-130.
[14]Shahla M.N., Farooq M.S., Naeem M.K., Riazuddin S.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 85-110.
[15]"Antioxidant peptides isolated from cheddar cheese made with adjunct culture L.casei ssp. casei 300."
Gupta A., Mann B., Kumar R., Sangwan R.B.
Submitted (MAR-2008) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 95-105, FUNCTION.
[16]"Nucleotide sequence of the 3'-nontranslated region of the mRNA of alpha S1-casein in cows."
Kiarshulite D.R., Zakhar'ev V.M., Gorodetskii S.I.
Dokl. Akad. Nauk SSSR 280:1433-1437(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 122-214.
[17]"Cloning, mapping, and sequencing of 3' and its flanking region of bovine alpha-s1 casein gene."
Chen R., Wang B., Zhang Y., Liu W., Zhang J., Lao W.
Chin. J. Biotechnol. 8:235-245(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 164-214.
[18]"Cloning of cDNA sequence coding for bovine alpha-s1-casein."
Maki M., Nagao M., Hirose M., Chiba H.
Agric. Biol. Chem. 47:441-444(1983)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 185-202.
Tissue: Mammary gland.
[19]"On the localization in the C-terminal sequence of bovine casein alpha-s1 of a Glu/Gly substitution that differentiates the genetic variants B and C."
Grosclaude F., Mercier J.-C., Ribadeau-Dumas B.
C. R. Hebd. Seances Acad. Sci., D, Sci. Nat. 268:3133-3136(1970)
Cited for: PROTEIN SEQUENCE OF 205-214 (VARIANT C).
[20]Erratum
Grosclaude F., Mercier J.-C., Ribadeau-Dumas B.
C. R. Hebd. Seances Acad. Sci., D, Sci. Nat. 271:563-563(1970)
Cited for: SEQUENCE REVISION.
[21]"Identification of IgE- and IgG-binding epitopes on alpha(s1)-casein: differences in patients with persistent and transient cow's milk allergy."
Chatchatee P., Jaervinen K.M., Bardina L., Beyer K., Sampson H.A.
J. Allergy Clin. Immunol. 107:379-383(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: ALLERGEN.
[22]"Highly selective enrichment of phosphorylated peptides from peptide mixtures using titanium dioxide microcolumns."
Larsen M.R., Thingholm T.E., Jensen O.N., Roepstorff P., Jorgensen T.J.
Mol. Cell. Proteomics 4:873-886(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-56; SER-61; SER-63; SER-79; SER-81; SER-82; SER-83; SER-90 AND SER-130, IDENTIFICATION BY MASS SPECTROMETRY.
[23]Bai F., Liu S., Witzmann F.A.
Submitted (SEP-2005) to UniProtKB
Cited for: PHOSPHORYLATION AT SER-56.

Web resources

Worthington enzyme manual
Protein Spotlight

Of buttons, digestion and glue - Issue 16 of November 2001

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X00564 mRNA. Translation: CAB57792.1.
M33123 mRNA. Translation: AAA30428.1.
M38641 mRNA. Translation: AAA30429.1.
X59856 Genomic DNA. Translation: CAA42516.1.
EU221551 Genomic DNA. Translation: ABW98936.1.
EU221552 Genomic DNA. Translation: ABW98937.1.
EU221553 Genomic DNA. Translation: ABW98938.1.
EU221554 Genomic DNA. Translation: ABW98939.1.
EU221555 Genomic DNA. Translation: ABW98940.1.
EU221556 Genomic DNA. Translation: ABW98941.1.
EU221557 Genomic DNA. Translation: ABW98942.1.
EU221558 Genomic DNA. Translation: ABW98943.1.
EU221559 Genomic DNA. Translation: ABW98944.1.
EU221560 Genomic DNA. Translation: ABW98945.1.
EU221561 Genomic DNA. Translation: ABW98946.1.
EU221562 Genomic DNA. Translation: ABW98947.1.
EU221563 Genomic DNA. Translation: ABW98948.1.
EU221564 Genomic DNA. Translation: ABW98949.1.
EU221565 Genomic DNA. Translation: ABW98950.1.
EU221566 Genomic DNA. Translation: ABW98951.1.
EU221567 Genomic DNA. Translation: ABW98952.1.
EU221568 Genomic DNA. Translation: ABW98953.1.
EU221569 Genomic DNA. Translation: ABW98954.1.
EU221570 Genomic DNA. Translation: ABW98955.1.
EU221571 Genomic DNA. Translation: ABW98956.1.
EU221572 Genomic DNA. Translation: ABW98957.1.
EU221573 Genomic DNA. Translation: ABW98958.1.
EU221574 Genomic DNA. Translation: ABW98959.1.
EU221575 Genomic DNA. Translation: ABW98960.1.
EU221576 Genomic DNA. Translation: ABW98961.1.
EU221577 Genomic DNA. Translation: ABW98962.1.
EU221578 Genomic DNA. Translation: ABW98963.1.
EU221579 Genomic DNA. Translation: ABW98964.1.
EU221580 Genomic DNA. Translation: ABW98965.1.
EU221581 Genomic DNA. Translation: ABW98966.1.
BC109618 mRNA. Translation: AAI09619.1.
K01084 mRNA. Translation: AAA30478.1.
EF538766 Genomic DNA. Translation: ABQ88318.1.
M38658 mRNA. Translation: AAA62707.1.
AH007360 Genomic DNA. Translation: AAD14099.1.
D00412 mRNA. Translation: BAA00313.1.
PIRKABOSB. S22575.
S72220.
RefSeqNP_851372.1. NM_181029.2.
UniGeneBt.59369.

3D structure databases

DisProtDP00330.
ProteinModelPortalP02662.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid159482. 3 interactions.
DIPDIP-29917N.
IntActP02662. 2 interactions.
MINTMINT-8184454.

Protein family/group databases

Allergome10197. Bos d 9.0101.
167. Bos d 8.
2734. Bos d 9.

Proteomic databases

PaxDbP02662.
PRIDEP02662.
ProMEXP02662.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000010119; ENSBTAP00000010119; ENSBTAG00000007695.
GeneID282208.
KEGGbta:282208.

Organism-specific databases

CTD1446.

Phylogenomic databases

eggNOGNOG73245.
GeneTreeENSGT00390000017378.
HOVERGENHBG005242.
InParanoidP02662.
KOK17281.
OrthoDBEOG7W1561.
TreeFamTF340763.

Family and domain databases

InterProIPR026999. Alpha-s1_casein.
IPR001588. Casein.
[Graphical view]
PANTHERPTHR10240. PTHR10240. 1 hit.
PfamPF00363. Casein. 1 hit.
[Graphical view]
PROSITEPS00306. CASEIN_ALPHA_BETA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20806033.
PMAP-CutDBP02662.

Entry information

Entry nameCASA1_BOVIN
AccessionPrimary (citable) accession number: P02662
Secondary accession number(s): A5YK80 expand/collapse secondary AC list , A8WCP1, A8WCP2, A8WCP3, A8WCP4, A8WCP5, A8WCP6, A8WCP7, A8WCP8, A8WCP9, A8WCQ0, A8WCQ1, A8WCQ2, A8WCQ3, A8WCQ4, A8WCQ5, A8WCQ6, A8WCQ7, A8WCQ8, A8WCQ9, A8WCR0, A8WCR1, A8WCR2, A8WCR3, A8WCR4, A8WCR5, A8WCR6, A8WCR7, A8WCR8, A8WCR9, A8WCS0, A8WCS1, Q28048, Q28069, Q32LE8, Q7M2U6, Q9TRH5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1990
Last modified: April 16, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries