Reviewed,
UniProtKB/Swiss-Prot P02662 (CASA1_BOVIN)
Last modified
June 16, 2009.
Version 88.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Alpha-S1-casein Cleaved into the following chain: 1- Recommended name: Antioxidant peptide | ||
| Gene names |
| ||
| Organism | Bos taurus (Bovine) | ||
| Taxonomic identifier | 9913 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Protein attributes
| Sequence length | 214 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Important role in the capacity of milk to transport calcium phosphate. Ref.15 Antioxidant peptide has 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Ref.15 |
| Subcellular location | |
| Tissue specificity | Mammary gland specific. Secreted in milk. |
| Miscellaneous | The B variant sequence is shown. |
| Sequence similarities | Belongs to the alpha-casein family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Secreted |
| Coding sequence diversity | Polymorphism |
| Domain | Repeat Signal |
| Molecular function | Antioxidant Milk protein |
| PTM | Phosphoprotein |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | transport Inferred from electronic annotation. Source: InterPro |
| Cellular component | extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | antioxidant activity Inferred from electronic annotation. Source: UniProtKB-KW transporter activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 15 | 15 | Ref.7 Ref.9 | ||||||
| Chain | 16 – 214 | 199 | Alpha-S1-casein | PRO_0000004446 | |||||
| Peptide | 95 – 105 | 11 | Antioxidant peptide | PRO_0000331578 | |||||
Regions | |||||||||
| Repeat | 85 – 99 | 15 | |||||||
| Repeat | 125 – 140 | 16 | |||||||
Amino acid modifications | |||||||||
| Modified residue | 56 | 1 | Phosphoserine Ref.21 Ref.22 | ||||||
| Modified residue | 61 | 1 | Phosphoserine Ref.22 | ||||||
| Modified residue | 63 | 1 | Phosphoserine Ref.22 | ||||||
| Modified residue | 68 | 1 | Phosphothreonine; in variant D | ||||||
| Modified residue | 79 | 1 | Phosphoserine Ref.22 | ||||||
| Modified residue | 81 | 1 | Phosphoserine Ref.22 | ||||||
| Modified residue | 82 | 1 | Phosphoserine Ref.22 | ||||||
| Modified residue | 83 | 1 | Phosphoserine Ref.22 | ||||||
| Modified residue | 90 | 1 | Phosphoserine Ref.22 | ||||||
| Modified residue | 130 | 1 | Phosphoserine Ref.22 | ||||||
Natural variations | |||||||||
| Natural variant | 29 – 41 | 13 | Missing in variant A. | ||||||
| Natural variant | 68 | 1 | A → T in variant D. | ||||||
| Natural variant | 207 | 1 | E → G in variant C. | ||||||
Experimental info | |||||||||
| Sequence conflict | 11 – 12 | 2 | AV → SA in ABW98943. Ref.5 | ||||||
| Sequence conflict | 42 | 1 | P → L in AAA30429. Ref.3 | ||||||
| Sequence conflict | 44 | 1 | P → S in ABW98945. Ref.5 | ||||||
| Sequence conflict | 95 | 1 | H → Q in AAA30478. Ref.13 | ||||||
| Sequence conflict | 99 | 1 | E → D in ABQ88318. Ref.14 | ||||||
| Sequence conflict | 143 | 1 | H → D in AAA30429. Ref.3 | ||||||
| Sequence conflict | 171 | 1 | L → P in ABW98953. Ref.5 | ||||||
| Sequence conflict | 203 | 1 | S → L in AAA62707. Ref.16 | ||||||
| Sequence conflict | 211 – 212 | 2 | MP → IS in AAA30429. Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Nucleotide sequences of bovine alpha S1- and kappa-casein cDNAs." Stewart A.F., Willis I.M., Mackinlay A.G. Nucleic Acids Res. 12:3895-3907(1984) [PubMed: 6328443] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Isolation and sequence analysis of bovine alpha-S1-casein cDNA clone." Nagao M., Maki M., Sasaki R., Chiba R. Agric. Biol. Chem. 48:1663-1667(1984) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "cDNA of cattle alpha S1-casein: cloning and nucleotide sequence." Gorodetskii S.I., Zakhar'ev V.M., Kyarshulite D.R., Kapelinskaya T.V., Skryabin K.G. Biokhimiia 51:1641-1648(1986) [PubMed: 3022833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Mammary gland. |
| [4] | "Genomic organization of the bovine alpha-S1 casein gene." Koczan D., Hobom G., Seyfert H.-M. Nucleic Acids Res. 19:5591-5596(1991) [PubMed: 1658736] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [5] | "Cloning, expression and mapping of allergenic determinants of alphaS1-casein, a major cow's milk allergen." Schulmeister U., Hochwallner H., Swoboda I., Focke-Tejkl M., Geller B., Nystrand M., Harlin A., Thalhamer J., Scheiblhofer S., Keller W., Niggemann B., Quirce S., Ebner C., Mari A., Pauli G., Herz U., Valenta R., Spitzauer S. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [6] | NIH - Mammalian Gene Collection (MGC) project Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver. |
| [7] | "Primary structure of bovine alpha-s1 casein. Complete sequence." Mercier J.-C., Grosclaude F., Ribadeau-Dumas B. Eur. J. Biochem. 23:41-51(1971) [PubMed: 4331376] [Abstract] Cited for: PROTEIN SEQUENCE OF 16-214 (VARIANT B). |
| [8] | "Primary structure of alpha casein and of bovine beta casein. Correction." Mercier J.-C., Grosclaude F., Ribadeau-Dumas B. Eur. J. Biochem. 40:323-323(1973) [PubMed: 4797901] [Abstract] Cited for: SEQUENCE REVISION TO 74 AND 92-93 (VARIANTS A; B; C AND D). |
| [9] | "Characterization of genetic variants of alpha-S1 and beta bovine caseins." Grosclaude F., Mahe M.-F., Mercier J.-C., Ribadeau-Dumas B. Eur. J. Biochem. 26:328-337(1972) [PubMed: 5064450] [Abstract] Cited for: PROTEIN SEQUENCE OF 16-214 (VARIANT D). |
| [10] | "Localization in the N-terminal part of bovine casein alpha-s1 of a 13 amino-acid deletion that differentiates variant A from variants B and C." Grosclaude F., Mahe M.-F., Mercier J.-C., Ribadeau-Dumas B. FEBS Lett. 11:109-112(1970) [PubMed: 11945462] [Abstract] Cited for: PROTEIN SEQUENCE OF 23-49 (VARIANT A). |
| [11] | "Isolation of a new ligand-carrying casein fragment from bovine mammary gland microsomes." Neuteboom B., Giuffrida M.G., Conti A. FEBS Lett. 305:189-191(1992) [PubMed: 1299613] [Abstract] Cited for: PROTEIN SEQUENCE OF 39-55. Tissue: Mammary gland. |
| [12] | "Action on bovine alpha s1-casein of cardosins A and B, aspartic proteinases from the flowers of the cardoon Cynara cardunculus L." Ramalho-Santos M., Verissimo P., Faro C., Pires E. Biochim. Biophys. Acta 1297:83-89(1996) [PubMed: 8841384] [Abstract] Cited for: PROTEIN SEQUENCE OF 39-47 AND 166-188. |
| [13] | "Construction and identification by partial nucleotide sequence analysis of bovine casein and beta-lactoglobulin cDNA clones." Willis I.M., Stewart A.F., Caputo A., Thompson A.R., McKinlay A.G. DNA 1:375-386(1982) [PubMed: 6897774] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 55-130. |
| [14] | Shahla M.N., Farooq M.S., Naeem M.K., Riazuddin S. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 85-110. |
| [15] | "Antioxidant peptides isolated from cheddar cheese made with adjunct culture L.casei ssp. casei 300." Gupta A., Mann B., Kumar R., Sangwan R.B. Submitted (MAR-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 95-105, FUNCTION. |
| [16] | "Nucleotide sequence of the 3'-nontranslated region of the mRNA of alpha S1-casein in cows." Kiarshulite D.R., Zakhar'ev V.M., Gorodetskii S.I. Dokl. Akad. Nauk SSSR 280:1433-1437(1985) [PubMed: 3838718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 122-214. |
| [17] | "Cloning, mapping, and sequencing of 3' and its flanking region of bovine alpha-s1 casein gene." Chen R., Wang B., Zhang Y., Liu W., Zhang J., Lao W. Chin. J. Biotechnol. 8:235-245(1992) [PubMed: 1343827] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 164-214. |
| [18] | "Cloning of cDNA sequence coding for bovine alpha-s1-casein." Maki M., Nagao M., Hirose M., Chiba H. Agric. Biol. Chem. 47:441-444(1983) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 185-202. Tissue: Mammary gland. |
| [19] | "On the localization in the C-terminal sequence of bovine casein alpha-s1 of a Glu/Gly substitution that differentiates the genetic variants B and C." Grosclaude F., Mercier J.-C., Ribadeau-Dumas B. C. R. Hebd. Seances Acad. Sci., D, Sci. Nat. 268:3133-3136(1970) Cited for: PROTEIN SEQUENCE OF 205-214 (VARIANT C). |
| [20] | Erratum Grosclaude F., Mercier J.-C., Ribadeau-Dumas B. C. R. Hebd. Seances Acad. Sci., D, Sci. Nat. 271:563-563(1970) Cited for: SEQUENCE REVISION. |
| [21] | Bai F., Liu S., Witzmann F.A. Submitted (SEP-2005) to UniProtKB Cited for: PHOSPHORYLATION AT SER-56. |
| [22] | "Reference-facilitated phosphoproteomics: fast and reliable phosphopeptide validation by micro LC-ESI-Q-TOF MS/MS." Imanishi S.Y., Kochin V., Ferraris S.E., de Thonel A., Pallari H.M., Corthals G.L., Eriksson J.E. Mol. Cell. Proteomics 6:1380-1391(2007) [PubMed: 17510049] [Abstract] Cited for: PHOSPHORYLATION AT SER-56; SER-61; SER-63; SER-79; SER-81; SER-82; SER-83; SER-90 AND SER-130, MASS SPECTROMETRY. |
Web resources
| Worthington enzyme manual |
| Protein Spotlight Of buttons, digestion and glue - Issue 16 of November 2001 |
Cross-references
Sequence databases | |
|---|---|
| X00564 mRNA. Translation: CAB57792.1. M33123 mRNA. Translation: AAA30428.1. M38641 mRNA. Translation: AAA30429.1. X59856 Genomic DNA. Translation: CAA42516.1. EU221551 Genomic DNA. Translation: ABW98936.1. EU221552 Genomic DNA. Translation: ABW98937.1. EU221553 Genomic DNA. Translation: ABW98938.1. EU221554 Genomic DNA. Translation: ABW98939.1. EU221555 Genomic DNA. Translation: ABW98940.1. EU221556 Genomic DNA. Translation: ABW98941.1. EU221557 Genomic DNA. Translation: ABW98942.1. EU221558 Genomic DNA. Translation: ABW98943.1. EU221559 Genomic DNA. Translation: ABW98944.1. EU221560 Genomic DNA. Translation: ABW98945.1. EU221561 Genomic DNA. Translation: ABW98946.1. EU221562 Genomic DNA. Translation: ABW98947.1. EU221563 Genomic DNA. Translation: ABW98948.1. EU221564 Genomic DNA. Translation: ABW98949.1. EU221565 Genomic DNA. Translation: ABW98950.1. EU221566 Genomic DNA. Translation: ABW98951.1. EU221567 Genomic DNA. Translation: ABW98952.1. EU221568 Genomic DNA. Translation: ABW98953.1. EU221569 Genomic DNA. Translation: ABW98954.1. EU221570 Genomic DNA. Translation: ABW98955.1. EU221571 Genomic DNA. Translation: ABW98956.1. EU221572 Genomic DNA. Translation: ABW98957.1. EU221573 Genomic DNA. Translation: ABW98958.1. EU221574 Genomic DNA. Translation: ABW98959.1. EU221575 Genomic DNA. Translation: ABW98960.1. EU221576 Genomic DNA. Translation: ABW98961.1. EU221577 Genomic DNA. Translation: ABW98962.1. EU221578 Genomic DNA. Translation: ABW98963.1. EU221579 Genomic DNA. Translation: ABW98964.1. EU221580 Genomic DNA. Translation: ABW98965.1. EU221581 Genomic DNA. Translation: ABW98966.1. BC109618 mRNA. Translation: AAI09619.1. K01084 mRNA. Translation: AAA30478.1. EF538766 Genomic DNA. Translation: ABQ88318.1. M38658 mRNA. Translation: AAA62707.1. S72388 Genomic DNA. Translation: AAD14099.1. D00412 mRNA. Translation: BAA00313.1. | |
| IPI | IPI00706094. |
| PIR | KABOSB. S22575. S72220. |
| RefSeq | NP_851372.1. |
| UniGene | Bt.59369 |
3D structure databases | |
| DisProt | DP00330. |
| ModBase | Search... |
Proteomic databases | |
| PRIDE | P02662. |
Genome annotation databases | |
| Ensembl | ENSBTAG00000007695. Bos taurus. [Contig view] |
| GeneID | 282208. |
| KEGG | bta:282208. |
Phylogenomic databases | |
| HOVERGEN | P02662. |
Family and domain databases | |
| InterPro | IPR001588. Casein. [Graphical view] |
| Pfam | PF00363. Casein. 1 hit. [Graphical view] |
| PROSITE | PS00306. CASEIN_ALPHA_BETA. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| PMAP-CutDB | P02662. |
Entry information
| Entry name | CASA1_BOVIN | ||||||||
| Accession | Primary (citable) accession number: P02662 Secondary accession number(s): A5YK80 Q9TRH5 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

Clusters with


