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Protein

Alpha-S1-casein

Gene

CSN1S1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Important role in the capacity of milk to transport calcium phosphate.1 Publication
Antioxidant peptide has 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity.1 Publication

GO - Molecular functioni

GO - Biological processi

  • negative regulation of apoptotic process Source: AgBase
  • positive regulation of androst-4-ene-3,17-dione biosynthetic process Source: AgBase
  • positive regulation of androstenedione secretion Source: AgBase
  • positive regulation of progesterone biosynthetic process Source: AgBase
  • positive regulation of progesterone secretion Source: AgBase
  • response to 11-deoxycorticosterone Source: AgBase
  • response to dehydroepiandrosterone Source: AgBase
  • response to estradiol Source: AgBase
  • response to growth hormone Source: AgBase
  • response to progesterone Source: AgBase
Complete GO annotation...

Keywords - Molecular functioni

Antioxidant, Milk protein

Enzyme and pathway databases

ReactomeiREACT_321619. Miscellaneous transport and binding events.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-S1-casein
Alternative name(s):
Allergen: Bos d 8
Cleaved into the following chain:
Gene namesi
Name:CSN1S1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 6

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: AgBase
  • Golgi apparatus Source: AgBase
  • Golgi lumen Source: AgBase
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Has six major and 3 minor IgE-binding regions and 5 major and 1 minor IgG-binding regions. Is a cause of cow's milk allergy (CMA).2 Publications

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei10197. Bos d 9.0101.
167. Bos d 8.
2734. Bos d 9.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 15152 PublicationsAdd
BLAST
Chaini16 – 214199Alpha-S1-caseinPRO_0000004446Add
BLAST
Peptidei95 – 10511Antioxidant peptidePRO_0000331578Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei56 – 561Phosphoserine2 Publications
Modified residuei61 – 611Phosphoserine1 Publication
Modified residuei63 – 631Phosphoserine1 Publication
Modified residuei79 – 791Phosphoserine1 Publication
Modified residuei81 – 811Phosphoserine1 Publication
Modified residuei82 – 821Phosphoserine1 Publication
Modified residuei83 – 831Phosphoserine1 Publication
Modified residuei90 – 901Phosphoserine1 Publication
Modified residuei130 – 1301Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP02662.
PRIDEiP02662.

Miscellaneous databases

PMAP-CutDBP02662.

Expressioni

Tissue specificityi

Mammary gland specific. Secreted in milk.

Gene expression databases

ExpressionAtlasiP02662. baseline.

Interactioni

Protein-protein interaction databases

BioGridi159482. 3 interactions.
DIPiDIP-29917N.
IntActiP02662. 2 interactions.
MINTiMINT-8184454.
STRINGi9913.ENSBTAP00000010119.

Structurei

3D structure databases

DisProtiDP00330.
ProteinModelPortaliP02662.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati85 – 9915Add
BLAST
Repeati125 – 14016Add
BLAST

Sequence similaritiesi

Belongs to the alpha-casein family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG73245.
GeneTreeiENSGT00390000017378.
HOVERGENiHBG005242.
InParanoidiP02662.
KOiK17281.
OMAiIVPNSAE.
OrthoDBiEOG7W1561.
TreeFamiTF340763.

Family and domain databases

InterProiIPR026999. Alpha-s1_casein.
IPR001588. Casein.
[Graphical view]
PANTHERiPTHR10240. PTHR10240. 1 hit.
PfamiPF00363. Casein. 1 hit.
[Graphical view]
PROSITEiPS00306. CASEIN_ALPHA_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02662-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLILTCLV AVALARPKHP IKHQGLPQEV LNENLLRFFV APFPEVFGKE
60 70 80 90 100
KVNELSKDIG SESTEDQAME DIKQMEAESI SSSEEIVPNS VEQKHIQKED
110 120 130 140 150
VPSERYLGYL EQLLRLKKYK VPQLEIVPNS AEERLHSMKE GIHAQQKEPM
160 170 180 190 200
IGVNQELAYF YPELFRQFYQ LDAYPSGAWY YVPLGTQYTD APSFSDIPNP
210
IGSENSEKTT MPLW
Length:214
Mass (Da):24,529
Last modified:November 1, 1990 - v2
Checksum:iF066B5C8AE55828B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 122AV → SA in ABW98943 (PubMed:19454699).Curated
Sequence conflicti42 – 421P → L in AAA30429 (PubMed:3022833).Curated
Sequence conflicti44 – 441P → S in ABW98945 (PubMed:19454699).Curated
Sequence conflicti95 – 951H → Q in AAA30478 (PubMed:6897774).Curated
Sequence conflicti99 – 991E → D in ABQ88318 (Ref. 14) Curated
Sequence conflicti143 – 1431H → D in AAA30429 (PubMed:3022833).Curated
Sequence conflicti171 – 1711L → P in ABW98953 (PubMed:19454699).Curated
Sequence conflicti203 – 2031S → L in AAA62707 (PubMed:3838718).Curated
Sequence conflicti211 – 2122MP → IS in AAA30429 (PubMed:3022833).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti29 – 4113Missing in variant A.
Add
BLAST
Natural varianti68 – 681A → T in variant D.
Natural varianti207 – 2071E → G in variant C.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00564 mRNA. Translation: CAB57792.1.
M33123 mRNA. Translation: AAA30428.1.
M38641 mRNA. Translation: AAA30429.1.
X59856 Genomic DNA. Translation: CAA42516.1.
EU221551 Genomic DNA. Translation: ABW98936.1.
EU221552 Genomic DNA. Translation: ABW98937.1.
EU221553 Genomic DNA. Translation: ABW98938.1.
EU221554 Genomic DNA. Translation: ABW98939.1.
EU221555 Genomic DNA. Translation: ABW98940.1.
EU221556 Genomic DNA. Translation: ABW98941.1.
EU221557 Genomic DNA. Translation: ABW98942.1.
EU221558 Genomic DNA. Translation: ABW98943.1.
EU221559 Genomic DNA. Translation: ABW98944.1.
EU221560 Genomic DNA. Translation: ABW98945.1.
EU221561 Genomic DNA. Translation: ABW98946.1.
EU221562 Genomic DNA. Translation: ABW98947.1.
EU221563 Genomic DNA. Translation: ABW98948.1.
EU221564 Genomic DNA. Translation: ABW98949.1.
EU221565 Genomic DNA. Translation: ABW98950.1.
EU221566 Genomic DNA. Translation: ABW98951.1.
EU221567 Genomic DNA. Translation: ABW98952.1.
EU221568 Genomic DNA. Translation: ABW98953.1.
EU221569 Genomic DNA. Translation: ABW98954.1.
EU221570 Genomic DNA. Translation: ABW98955.1.
EU221571 Genomic DNA. Translation: ABW98956.1.
EU221572 Genomic DNA. Translation: ABW98957.1.
EU221573 Genomic DNA. Translation: ABW98958.1.
EU221574 Genomic DNA. Translation: ABW98959.1.
EU221575 Genomic DNA. Translation: ABW98960.1.
EU221576 Genomic DNA. Translation: ABW98961.1.
EU221577 Genomic DNA. Translation: ABW98962.1.
EU221578 Genomic DNA. Translation: ABW98963.1.
EU221579 Genomic DNA. Translation: ABW98964.1.
EU221580 Genomic DNA. Translation: ABW98965.1.
EU221581 Genomic DNA. Translation: ABW98966.1.
BC109618 mRNA. Translation: AAI09619.1.
K01084 mRNA. Translation: AAA30478.1.
EF538766 Genomic DNA. Translation: ABQ88318.1.
M38658 mRNA. Translation: AAA62707.1.
AH007360 Genomic DNA. Translation: AAD14099.1.
D00412 mRNA. Translation: BAA00313.1.
PIRiS22575. KABOSB.
S72220.
RefSeqiNP_851372.1. NM_181029.2.
UniGeneiBt.59369.

Genome annotation databases

EnsembliENSBTAT00000010119; ENSBTAP00000010119; ENSBTAG00000007695.
GeneIDi282208.
KEGGibta:282208.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Worthington enzyme manual
Protein Spotlight

Of buttons, digestion and glue - Issue 16 of November 2001

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00564 mRNA. Translation: CAB57792.1.
M33123 mRNA. Translation: AAA30428.1.
M38641 mRNA. Translation: AAA30429.1.
X59856 Genomic DNA. Translation: CAA42516.1.
EU221551 Genomic DNA. Translation: ABW98936.1.
EU221552 Genomic DNA. Translation: ABW98937.1.
EU221553 Genomic DNA. Translation: ABW98938.1.
EU221554 Genomic DNA. Translation: ABW98939.1.
EU221555 Genomic DNA. Translation: ABW98940.1.
EU221556 Genomic DNA. Translation: ABW98941.1.
EU221557 Genomic DNA. Translation: ABW98942.1.
EU221558 Genomic DNA. Translation: ABW98943.1.
EU221559 Genomic DNA. Translation: ABW98944.1.
EU221560 Genomic DNA. Translation: ABW98945.1.
EU221561 Genomic DNA. Translation: ABW98946.1.
EU221562 Genomic DNA. Translation: ABW98947.1.
EU221563 Genomic DNA. Translation: ABW98948.1.
EU221564 Genomic DNA. Translation: ABW98949.1.
EU221565 Genomic DNA. Translation: ABW98950.1.
EU221566 Genomic DNA. Translation: ABW98951.1.
EU221567 Genomic DNA. Translation: ABW98952.1.
EU221568 Genomic DNA. Translation: ABW98953.1.
EU221569 Genomic DNA. Translation: ABW98954.1.
EU221570 Genomic DNA. Translation: ABW98955.1.
EU221571 Genomic DNA. Translation: ABW98956.1.
EU221572 Genomic DNA. Translation: ABW98957.1.
EU221573 Genomic DNA. Translation: ABW98958.1.
EU221574 Genomic DNA. Translation: ABW98959.1.
EU221575 Genomic DNA. Translation: ABW98960.1.
EU221576 Genomic DNA. Translation: ABW98961.1.
EU221577 Genomic DNA. Translation: ABW98962.1.
EU221578 Genomic DNA. Translation: ABW98963.1.
EU221579 Genomic DNA. Translation: ABW98964.1.
EU221580 Genomic DNA. Translation: ABW98965.1.
EU221581 Genomic DNA. Translation: ABW98966.1.
BC109618 mRNA. Translation: AAI09619.1.
K01084 mRNA. Translation: AAA30478.1.
EF538766 Genomic DNA. Translation: ABQ88318.1.
M38658 mRNA. Translation: AAA62707.1.
AH007360 Genomic DNA. Translation: AAD14099.1.
D00412 mRNA. Translation: BAA00313.1.
PIRiS22575. KABOSB.
S72220.
RefSeqiNP_851372.1. NM_181029.2.
UniGeneiBt.59369.

3D structure databases

DisProtiDP00330.
ProteinModelPortaliP02662.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi159482. 3 interactions.
DIPiDIP-29917N.
IntActiP02662. 2 interactions.
MINTiMINT-8184454.
STRINGi9913.ENSBTAP00000010119.

Protein family/group databases

Allergomei10197. Bos d 9.0101.
167. Bos d 8.
2734. Bos d 9.

Proteomic databases

PaxDbiP02662.
PRIDEiP02662.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000010119; ENSBTAP00000010119; ENSBTAG00000007695.
GeneIDi282208.
KEGGibta:282208.

Organism-specific databases

CTDi1446.

Phylogenomic databases

eggNOGiNOG73245.
GeneTreeiENSGT00390000017378.
HOVERGENiHBG005242.
InParanoidiP02662.
KOiK17281.
OMAiIVPNSAE.
OrthoDBiEOG7W1561.
TreeFamiTF340763.

Enzyme and pathway databases

ReactomeiREACT_321619. Miscellaneous transport and binding events.

Miscellaneous databases

NextBioi20806033.
PMAP-CutDBP02662.
PROiP02662.

Gene expression databases

ExpressionAtlasiP02662. baseline.

Family and domain databases

InterProiIPR026999. Alpha-s1_casein.
IPR001588. Casein.
[Graphical view]
PANTHERiPTHR10240. PTHR10240. 1 hit.
PfamiPF00363. Casein. 1 hit.
[Graphical view]
PROSITEiPS00306. CASEIN_ALPHA_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequences of bovine alpha S1- and kappa-casein cDNAs."
    Stewart A.F., Willis I.M., Mackinlay A.G.
    Nucleic Acids Res. 12:3895-3907(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation and sequence analysis of bovine alpha-S1-casein cDNA clone."
    Nagao M., Maki M., Sasaki R., Chiba R.
    Agric. Biol. Chem. 48:1663-1667(1984)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "cDNA of cattle alpha S1-casein: cloning and nucleotide sequence."
    Gorodetskii S.I., Zakhar'ev V.M., Kyarshulite D.R., Kapelinskaya T.V., Skryabin K.G.
    Biokhimiia 51:1641-1648(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Mammary gland.
  4. "Genomic organization of the bovine alpha-S1 casein gene."
    Koczan D., Hobom G., Seyfert H.-M.
    Nucleic Acids Res. 19:5591-5596(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALLERGEN.
  6. NIH - Mammalian Gene Collection (MGC) project
    Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.
  7. "Primary structure of bovine alpha-s1 casein. Complete sequence."
    Mercier J.-C., Grosclaude F., Ribadeau-Dumas B.
    Eur. J. Biochem. 23:41-51(1971) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 16-214 (VARIANT B).
  8. "Primary structure of alpha casein and of bovine beta casein. Correction."
    Mercier J.-C., Grosclaude F., Ribadeau-Dumas B.
    Eur. J. Biochem. 40:323-323(1973) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 74 AND 92-93 (VARIANTS A; B; C AND D).
  9. "Characterization of genetic variants of alpha-S1 and beta bovine caseins."
    Grosclaude F., Mahe M.-F., Mercier J.-C., Ribadeau-Dumas B.
    Eur. J. Biochem. 26:328-337(1972) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 16-214 (VARIANT D).
  10. "Localization in the N-terminal part of bovine casein alpha-s1 of a 13 amino-acid deletion that differentiates variant A from variants B and C."
    Grosclaude F., Mahe M.-F., Mercier J.-C., Ribadeau-Dumas B.
    FEBS Lett. 11:109-112(1970) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 23-49 (VARIANT A).
  11. "Isolation of a new ligand-carrying casein fragment from bovine mammary gland microsomes."
    Neuteboom B., Giuffrida M.G., Conti A.
    FEBS Lett. 305:189-191(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 39-55.
    Tissue: Mammary gland.
  12. "Action on bovine alpha s1-casein of cardosins A and B, aspartic proteinases from the flowers of the cardoon Cynara cardunculus L."
    Ramalho-Santos M., Verissimo P., Faro C., Pires E.
    Biochim. Biophys. Acta 1297:83-89(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 39-47 AND 166-188.
  13. "Construction and identification by partial nucleotide sequence analysis of bovine casein and beta-lactoglobulin cDNA clones."
    Willis I.M., Stewart A.F., Caputo A., Thompson A.R., McKinlay A.G.
    DNA 1:375-386(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 55-130.
  14. Shahla M.N., Farooq M.S., Naeem M.K., Riazuddin S.
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 85-110.
  15. "Antioxidant peptides isolated from cheddar cheese made with adjunct culture L.casei ssp. casei 300."
    Gupta A., Mann B., Kumar R., Sangwan R.B.
    Submitted (MAR-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 95-105, FUNCTION.
  16. "Nucleotide sequence of the 3'-nontranslated region of the mRNA of alpha S1-casein in cows."
    Kiarshulite D.R., Zakhar'ev V.M., Gorodetskii S.I.
    Dokl. Akad. Nauk SSSR 280:1433-1437(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 122-214.
  17. "Cloning, mapping, and sequencing of 3' and its flanking region of bovine alpha-s1 casein gene."
    Chen R., Wang B., Zhang Y., Liu W., Zhang J., Lao W.
    Chin. J. Biotechnol. 8:235-245(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 164-214.
  18. "Cloning of cDNA sequence coding for bovine alpha-s1-casein."
    Maki M., Nagao M., Hirose M., Chiba H.
    Agric. Biol. Chem. 47:441-444(1983)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 185-202.
    Tissue: Mammary gland.
  19. "On the localization in the C-terminal sequence of bovine casein alpha-s1 of a Glu/Gly substitution that differentiates the genetic variants B and C."
    Grosclaude F., Mercier J.-C., Ribadeau-Dumas B.
    C. R. Hebd. Seances Acad. Sci., D, Sci. Nat. 268:3133-3136(1970)
    Cited for: PROTEIN SEQUENCE OF 205-214 (VARIANT C).
  20. Erratum
    Grosclaude F., Mercier J.-C., Ribadeau-Dumas B.
    C. R. Hebd. Seances Acad. Sci., D, Sci. Nat. 271:563-563(1970)
    Cited for: SEQUENCE REVISION.
  21. "Identification of IgE- and IgG-binding epitopes on alpha(s1)-casein: differences in patients with persistent and transient cow's milk allergy."
    Chatchatee P., Jaervinen K.M., Bardina L., Beyer K., Sampson H.A.
    J. Allergy Clin. Immunol. 107:379-383(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALLERGEN.
  22. "Highly selective enrichment of phosphorylated peptides from peptide mixtures using titanium dioxide microcolumns."
    Larsen M.R., Thingholm T.E., Jensen O.N., Roepstorff P., Jorgensen T.J.
    Mol. Cell. Proteomics 4:873-886(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-56; SER-61; SER-63; SER-79; SER-81; SER-82; SER-83; SER-90 AND SER-130, IDENTIFICATION BY MASS SPECTROMETRY.
  23. Bai F., Liu S., Witzmann F.A.
    Submitted (SEP-2005) to UniProtKB
    Cited for: PHOSPHORYLATION AT SER-56.

Entry informationi

Entry nameiCASA1_BOVIN
AccessioniPrimary (citable) accession number: P02662
Secondary accession number(s): A5YK80
, A8WCP1, A8WCP2, A8WCP3, A8WCP4, A8WCP5, A8WCP6, A8WCP7, A8WCP8, A8WCP9, A8WCQ0, A8WCQ1, A8WCQ2, A8WCQ3, A8WCQ4, A8WCQ5, A8WCQ6, A8WCQ7, A8WCQ8, A8WCQ9, A8WCR0, A8WCR1, A8WCR2, A8WCR3, A8WCR4, A8WCR5, A8WCR6, A8WCR7, A8WCR8, A8WCR9, A8WCS0, A8WCS1, Q28048, Q28069, Q32LE8, Q7M2U6, Q9TRH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1990
Last modified: July 22, 2015
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The B variant sequence is shown.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.