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Protein

Apolipoprotein A-IV

Gene

Apoa4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.

GO - Molecular functioni

  1. cholesterol binding Source: GO_Central
  2. cholesterol transporter activity Source: GO_Central
  3. phosphatidylcholine binding Source: GO_Central
  4. phosphatidylcholine-sterol O-acyltransferase activator activity Source: GO_Central

GO - Biological processi

  1. cholesterol biosynthetic process Source: GO_Central
  2. cholesterol efflux Source: GO_Central
  3. cholesterol homeostasis Source: GO_Central
  4. high-density lipoprotein particle assembly Source: GO_Central
  5. lipid transport Source: RGD
  6. lipoprotein metabolic process Source: GO_Central
  7. peripheral nervous system axon regeneration Source: RGD
  8. phosphatidylcholine metabolic process Source: GO_Central
  9. phospholipid efflux Source: GO_Central
  10. positive regulation of cholesterol esterification Source: GO_Central
  11. positive regulation of fatty acid biosynthetic process Source: GO_Central
  12. positive regulation of lipoprotein lipase activity Source: GO_Central
  13. positive regulation of triglyceride catabolic process Source: GO_Central
  14. regulation of intestinal cholesterol absorption Source: GO_Central
  15. response to food Source: RGD
  16. response to triglyceride Source: RGD
  17. reverse cholesterol transport Source: GO_Central
  18. triglyceride homeostasis Source: GO_Central
  19. very-low-density lipoprotein particle remodeling Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Lipid transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Apolipoprotein A-IV
Short name:
Apo-AIV
Short name:
ApoA-IV
Alternative name(s):
Apolipoprotein A4
Gene namesi
Name:Apoa4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2132. Apoa4.

Subcellular locationi

GO - Cellular componenti

  1. blood microparticle Source: GO_Central
  2. chylomicron Source: RGD
  3. high-density lipoprotein particle Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Chylomicron, HDL, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Add
BLAST
Chaini21 – 391371Apolipoprotein A-IVPRO_0000001980Add
BLAST

Proteomic databases

PaxDbiP02651.
PRIDEiP02651.

PTM databases

PhosphoSiteiP02651.

Expressioni

Tissue specificityi

Secreted in plasma.

Gene expression databases

GenevestigatoriP02651.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP02651. 1 interaction.
STRINGi10116.ENSRNOP00000056172.

Structurei

3D structure databases

ProteinModelPortaliP02651.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati33 – 54221Add
BLAST
Repeati60 – 81222Add
BLAST
Repeati82 – 103223Add
BLAST
Repeati115 – 136224Add
BLAST
Repeati137 – 158225Add
BLAST
Repeati159 – 180226Add
BLAST
Repeati181 – 202227Add
BLAST
Repeati203 – 224228Add
BLAST
Repeati225 – 246229Add
BLAST
Repeati247 – 2682210Add
BLAST
Repeati269 – 2861811Add
BLAST
Repeati287 – 3082212Add
BLAST
Repeati309 – 3302213Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni33 – 33029813 X 22 AA approximate tandem repeatsAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi374 – 38512Gln/Glu-richAdd
BLAST

Domaini

Nine of the thirteen 22-amino acid tandem repeats (each 22-mer is actually a tandem array of two, A and B, related 11-mers) occurring in this sequence are predicted to be highly alpha-helical, and many of these helices are amphipathic. They may therefore serve as lipid-binding domains with lecithin:cholesterol acyltransferase (LCAT) activating abilities.

Sequence similaritiesi

Belongs to the apolipoprotein A1/A4/E family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOVERGENiHBG105707.
InParanoidiP02651.
KOiK08760.
PhylomeDBiP02651.

Family and domain databases

InterProiIPR000074. ApoA1_A4_E.
[Graphical view]
PfamiPF01442. Apolipoprotein. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02651-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFLKAVVLTV ALVAITGTQA EVTSDQVANV MWDYFTQLSN NAKEAVEQLQ
60 70 80 90 100
KTDVTQQLNT LFQDKLGNIN TYADDLQNKL VPFAVQLSGH LTKETERVRE
110 120 130 140 150
EIQKELEDLR ANMMPHANKV SQMFGDNVQK LQEHLRPYAT DLQAQINAQT
160 170 180 190 200
QDMKRQLTPY IQRMQTTIQD NVENLQSSMV PFANELKEKF NQNMEGLKGQ
210 220 230 240 250
LTPRANELKA TIDQNLEDLR SRLAPLAEGV QEKLNHQMEG LAFQMKKNAE
260 270 280 290 300
ELQTKVSTNI DQLQKNLAPL VEDVQSKLKG NTEGLQKSLE DLNKQLDQQV
310 320 330 340 350
EVFRRAVEPL GDKFNMALVQ QMEKFRQQLG SDSGDVESHL SFLEKNLREK
360 370 380 390
VSSFMSTLQK KGSPDQPLAL PLPEQVQEQV QEQVQPKPLE S
Length:391
Mass (Da):44,456
Last modified:July 1, 1989 - v2
Checksum:i24095004A809201D
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti253 – 2531Q → H.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M00002 mRNA. Translation: AAA85909.1.
J02588 Genomic DNA. Translation: AAA40747.1.
M13508 Genomic DNA. Translation: AAA40748.1.
BC091159 mRNA. Translation: AAH91159.1.
PIRiA03095. LPRTA4.
RefSeqiNP_036869.1. NM_012737.1.
UniGeneiRn.15739.

Genome annotation databases

GeneIDi25080.
KEGGirno:25080.
UCSCiRGD:2132. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M00002 mRNA. Translation: AAA85909.1.
J02588 Genomic DNA. Translation: AAA40747.1.
M13508 Genomic DNA. Translation: AAA40748.1.
BC091159 mRNA. Translation: AAH91159.1.
PIRiA03095. LPRTA4.
RefSeqiNP_036869.1. NM_012737.1.
UniGeneiRn.15739.

3D structure databases

ProteinModelPortaliP02651.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP02651. 1 interaction.
STRINGi10116.ENSRNOP00000056172.

PTM databases

PhosphoSiteiP02651.

Proteomic databases

PaxDbiP02651.
PRIDEiP02651.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25080.
KEGGirno:25080.
UCSCiRGD:2132. rat.

Organism-specific databases

CTDi337.
RGDi2132. Apoa4.

Phylogenomic databases

HOVERGENiHBG105707.
InParanoidiP02651.
KOiK08760.
PhylomeDBiP02651.

Miscellaneous databases

NextBioi605330.
PROiP02651.

Gene expression databases

GenevestigatoriP02651.

Family and domain databases

InterProiIPR000074. ApoA1_A4_E.
[Graphical view]
PfamiPF01442. Apolipoprotein. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Evolution of the apolipoproteins. Structure of the rat apo-A-IV gene and its relationship to the human genes for apo-A-I, C-III, and E."
    Boguski M.S., Birkenmeier E.H., Elshourbagy N.A., Taylor J.M., Gordon J.I.
    J. Biol. Chem. 261:6398-6407(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Rat apolipoprotein A-IV contains 13 tandem repetitions of a 22-amino acid segment with amphipathic helical potential."
    Boguski M.S., Elshourbagy N.A., Taylor J.M., Gordon J.I.
    Proc. Natl. Acad. Sci. U.S.A. 81:5021-5025(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Linkage, evolution, and expression of the rat apolipoprotein A-I, C-III, and A-IV genes."
    Haddad I.A., Ordovas J.M., Fitzpatrick T., Karathanasis S.K.
    J. Biol. Chem. 261:13268-13277(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiAPOA4_RAT
AccessioniPrimary (citable) accession number: P02651
Secondary accession number(s): Q5BK92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1989
Last modified: February 4, 2015
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.