P02651 (APOA4_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Apolipoprotein A-IV
Short name=Apo-AIV
Short name=ApoA-IV

Alternative name(s):
Apolipoprotein A4

Gene names

Name:

Apoa4

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.
Subcellular location	Secreted.
Tissue specificity	Secreted in plasma.
Domain	Nine of the thirteen 22-amino acid tandem repeats (each 22-mer is actually a tandem array of two, A and B, related 11-mers) occurring in this sequence are predicted to be highly alpha-helical, and many of these helices are amphipathic. They may therefore serve as lipid-binding domains with lecithin:cholesterol acyltransferase (LCAT) activating abilities.
Sequence similarities	Belongs to the apolipoprotein A1/A4/E family.

Ontologies

Keywords
Biological process	Lipid transport Transport
Cellular component	Chylomicron HDL Secreted
Domain	Repeat Signal
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipid transport Traceable author statement Ref.3. Source: RGD lipoprotein metabolic process Inferred from electronic annotation. Source: InterPro peripheral nervous system axon regeneration Inferred from expression pattern PubMed 2120218. Source: RGD response to food Traceable author statement PubMed 15059955. Source: RGD response to triglyceride Inferred from expression pattern PubMed 21472381. Source: RGD
Cellular_component	chylomicron Inferred from direct assay Ref.2. Source: RGD high-density lipoprotein particle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	lipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20
	Chain	21 – 391	371	Apolipoprotein A-IV		PRO_0000001980
Regions
	Repeat	33 – 54	22	1
	Repeat	60 – 81	22	2
	Repeat	82 – 103	22	3
	Repeat	115 – 136	22	4
	Repeat	137 – 158	22	5
	Repeat	159 – 180	22	6
	Repeat	181 – 202	22	7
	Repeat	203 – 224	22	8
	Repeat	225 – 246	22	9
	Repeat	247 – 268	22	10
	Repeat	269 – 286	18	11
	Repeat	287 – 308	22	12
	Repeat	309 – 330	22	13
	Region	33 – 330	298	13 X 22 AA approximate tandem repeats
	Compositional bias	374 – 385	12	Gln/Glu-rich
Natural variations
	Natural variant	253	1	Q → H.

Sequences

	Sequence		Length	Mass (Da)
	P02651 [UniParc]. Last modified July 1, 1989. Version 2. Checksum: 24095004A809201D	FASTA	391	44,456
	10 20 30 40 50 60 MFLKAVVLTV ALVAITGTQA EVTSDQVANV MWDYFTQLSN NAKEAVEQLQ KTDVTQQLNT 70 80 90 100 110 120 LFQDKLGNIN TYADDLQNKL VPFAVQLSGH LTKETERVRE EIQKELEDLR ANMMPHANKV 130 140 150 160 170 180 SQMFGDNVQK LQEHLRPYAT DLQAQINAQT QDMKRQLTPY IQRMQTTIQD NVENLQSSMV 190 200 210 220 230 240 PFANELKEKF NQNMEGLKGQ LTPRANELKA TIDQNLEDLR SRLAPLAEGV QEKLNHQMEG 250 260 270 280 290 300 LAFQMKKNAE ELQTKVSTNI DQLQKNLAPL VEDVQSKLKG NTEGLQKSLE DLNKQLDQQV 310 320 330 340 350 360 EVFRRAVEPL GDKFNMALVQ QMEKFRQQLG SDSGDVESHL SFLEKNLREK VSSFMSTLQK 370 380 390 KGSPDQPLAL PLPEQVQEQV QEQVQPKPLE S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evolution of the apolipoproteins. Structure of the rat apo-A-IV gene and its relationship to the human genes for apo-A-I, C-III, and E." Boguski M.S., Birkenmeier E.H., Elshourbagy N.A., Taylor J.M., Gordon J.I. J. Biol. Chem. 261:6398-6407(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Rat apolipoprotein A-IV contains 13 tandem repetitions of a 22-amino acid segment with amphipathic helical potential." Boguski M.S., Elshourbagy N.A., Taylor J.M., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 81:5021-5025(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Linkage, evolution, and expression of the rat apolipoprotein A-I, C-III, and A-IV genes." Haddad I.A., Ordovas J.M., Fitzpatrick T., Karathanasis S.K. J. Biol. Chem. 261:13268-13277(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M00002 mRNA. Translation: AAA85909.1. J02588 Genomic DNA. Translation: AAA40747.1. M13508 Genomic DNA. Translation: AAA40748.1. BC091159 mRNA. Translation: AAH91159.1.
IPI	IPI00324272.
PIR	LPRTA4. A03095.
RefSeq	NP_036869.1. NM_012737.1.
UniGene	Rn.15739.
3D structure databases
ProteinModelPortal	P02651.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000056172.
PTM databases
PhosphoSite	P02651.
Proteomic databases
PaxDb	P02651.
PRIDE	P02651.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	25080.
KEGG	rno:25080.
UCSC	RGD:2132. rat.
Organism-specific databases
CTD	337.
RGD	2132. Apoa4.
Phylogenomic databases
HOVERGEN	HBG105707.
KO	K08760.
Gene expression databases
Genevestigator	P02651.
Family and domain databases
InterPro	IPR013326. ApoA. IPR000074. ApoA1_A4_E. [Graphical view]
Pfam	PF01442. Apolipoprotein. 3 hits. [Graphical view]
SUPFAM	SSF47162. SSF47162. 3 hits.
ProtoNet	Search...
Other
NextBio	605330.

Entry information

Entry name

APOA4_RAT

Accession

Primary (citable) accession number: P02651
Secondary accession number(s): Q5BK92

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program