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Protein

Apolipoprotein E

Gene

Apoe

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.By similarity

GO - Molecular functioni

GO - Biological processi

  • aging Source: RGD
  • cellular response to cholesterol Source: RGD
  • cellular response to growth factor stimulus Source: RGD
  • cellular response to interleukin-1 Source: RGD
  • cholesterol catabolic process Source: GO_Central
  • cholesterol efflux Source: GO_Central
  • cholesterol homeostasis Source: GO_Central
  • high-density lipoprotein particle assembly Source: GO_Central
  • lipid transport Source: RGD
  • lipoprotein catabolic process Source: GO_Central
  • lipoprotein metabolic process Source: RGD
  • negative regulation of cholesterol biosynthetic process Source: RGD
  • negative regulation of neuron apoptotic process Source: RGD
  • neuron projection regeneration Source: RGD
  • oligodendrocyte differentiation Source: RGD
  • peripheral nervous system axon regeneration Source: RGD
  • phospholipid efflux Source: GO_Central
  • positive regulation of axon extension Source: RGD
  • positive regulation of cholesterol esterification Source: GO_Central
  • positive regulation of lipid biosynthetic process Source: GO_Central
  • positive regulation of nitric-oxide synthase activity Source: GO_Central
  • regulation of beta-amyloid clearance Source: GO_Central
  • regulation of cholesterol transport Source: GO_Central
  • regulation of triglyceride metabolic process Source: RGD
  • response to ethanol Source: RGD
  • response to insulin Source: RGD
  • response to retinoic acid Source: RGD
  • response to zinc ion Source: RGD
  • reverse cholesterol transport Source: GO_Central
  • triglyceride catabolic process Source: GO_Central
  • very-low-density lipoprotein particle remodeling Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

Heparin-binding

Enzyme and pathway databases

ReactomeiR-RNO-174800. Chylomicron-mediated lipid transport.
R-RNO-194223. HDL-mediated lipid transport.
R-RNO-3000480. Scavenging by Class A Receptors.
R-RNO-975634. Retinoid metabolism and transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Apolipoprotein E
Short name:
Apo-E
Gene namesi
Name:Apoe
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi2138. Apoe.

Subcellular locationi

  • Secreted By similarity

GO - Cellular componenti

  • blood microparticle Source: GO_Central
  • cell surface Source: RGD
  • chylomicron Source: GO_Central
  • cytoplasm Source: RGD
  • dendrite Source: RGD
  • discoidal high-density lipoprotein particle Source: RGD
  • endoplasmic reticulum Source: RGD
  • endosome Source: RGD
  • extracellular space Source: RGD
  • extrinsic component of external side of plasma membrane Source: RGD
  • Golgi apparatus Source: RGD
  • intermediate-density lipoprotein particle Source: GO_Central
  • late endosome Source: RGD
  • lysosome Source: RGD
  • neuronal cell body Source: RGD
  • nuclear envelope Source: RGD
  • plasma membrane Source: RGD
  • very-low-density lipoprotein particle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chylomicron, HDL, Secreted, VLDL

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Add BLAST18
ChainiPRO_000000199619 – 312Apolipoprotein EAdd BLAST294

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi25O-linked (GalNAc...)By similarity1
Modified residuei135Methionine sulfoxideBy similarity1
Modified residuei139PhosphoserineCombined sources1
Glycosylationi305O-linked (GalNAc...)By similarity1

Post-translational modificationi

Phosphorylated by FAM20C in the extracellular medium.By similarity

Keywords - PTMi

Glycoprotein, Oxidation, Phosphoprotein

Proteomic databases

PaxDbiP02650.
PRIDEiP02650.

PTM databases

iPTMnetiP02650.
PhosphoSitePlusiP02650.

Expressioni

Tissue specificityi

Secreted in plasma.

Gene expression databases

BgeeiENSRNOG00000018454.
ExpressionAtlasiP02650. baseline and differential.
GenevisibleiP02650. RN.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi247757. 1 interactor.
IntActiP02650. 3 interactors.
MINTiMINT-4585732.
STRINGi10116.ENSRNOP00000050968.

Structurei

3D structure databases

ProteinModelPortaliP02650.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati72 – 931Add BLAST22
Repeati94 – 1152Add BLAST22
Repeati116 – 1373Add BLAST22
Repeati138 – 1594Add BLAST22
Repeati160 – 1815Add BLAST22
Repeati182 – 2036Add BLAST22
Repeati204 – 2257Add BLAST22
Repeati226 – 2478Add BLAST22

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni72 – 2478 X 22 AA approximate tandem repeatsAdd BLAST176
Regioni150 – 160LDL receptor bindingBy similarityAdd BLAST11
Regioni154 – 157Heparin-bindingBy similarity4
Regioni221 – 228Heparin-bindingBy similarity8

Sequence similaritiesi

Belongs to the apolipoprotein A1/A4/E family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IVK0. Eukaryota.
ENOG4111MYC. LUCA.
GeneTreeiENSGT00730000111315.
HOGENOMiHOG000034006.
HOVERGENiHBG010582.
InParanoidiP02650.
KOiK04524.
PhylomeDBiP02650.
TreeFamiTF334458.

Family and domain databases

InterProiIPR000074. ApoA_E.
[Graphical view]
PfamiPF01442. Apolipoprotein. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02650-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKALWALLLV PLLTGCLAEG ELEVTDQLPG QSDQPWEQAL NRFWDYLRWV
60 70 80 90 100
QTLSDQVQEE LQSSQVTQEL TVLMEDTMTE VKAYKKELEE QLGPVAEETR
110 120 130 140 150
ARLAKEVQAA QARLGADMED LRNRLGQYRN EVNTMLGQST EELRSRLSTH
160 170 180 190 200
LRKMRKRLMR DADDLQKRLA VYKAGAQEGA ERGVSAIRER LGPLVEQGRQ
210 220 230 240 250
RTANLGAGAA QPLRDRAQAL SDRIRGRLEE VGNQARDRLE EVREQMEEVR
260 270 280 290 300
SKMEEQTQQI RLQAEIFQAR IKGWFEPLVE DMQRQWANLM EKIQASVATN
310
SIASTTVPLE NQ
Length:312
Mass (Da):35,753
Last modified:October 1, 1996 - v2
Checksum:i8180EEE933378D92
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti104A → T in CAA28650 (PubMed:3797247).Curated1
Sequence conflicti110A → T no nucleotide entry (PubMed:6190813).Curated1
Sequence conflicti110A → T in AAC60703 (PubMed:7766086).Curated1
Sequence conflicti141E → D no nucleotide entry (PubMed:6190813).Curated1
Sequence conflicti141E → D in AAC60703 (PubMed:7766086).Curated1
Sequence conflicti206 – 213GAGAAQPL → RWRRPAP in CAA28650 (PubMed:3797247).Curated8
Sequence conflicti206 – 213GAGAAQPL → RWRRPAP no nucleotide entry (PubMed:6190813).Curated8
Sequence conflicti309 – 310LE → WR no nucleotide entry (PubMed:6190813).Curated2
Sequence conflicti309 – 310LE → WR in AAC60703 (PubMed:7766086).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04979 Genomic DNA. Translation: CAA28650.1.
J02582 Genomic DNA. Translation: AAA40755.1.
S76779 mRNA. Translation: AAC60703.1.
BC086581 mRNA. Translation: AAH86581.1.
PIRiA26189. LPRTE.
RefSeqiNP_001257610.1. NM_001270681.1.
NP_001257611.1. NM_001270682.1.
NP_001257612.1. NM_001270683.1.
NP_001257613.1. NM_001270684.1.
NP_620183.2. NM_138828.3.
UniGeneiRn.32351.

Genome annotation databases

EnsembliENSRNOT00000041891; ENSRNOP00000050968; ENSRNOG00000018454.
ENSRNOT00000091574; ENSRNOP00000068937; ENSRNOG00000018454.
GeneIDi25728.
KEGGirno:25728.
UCSCiRGD:2138. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04979 Genomic DNA. Translation: CAA28650.1.
J02582 Genomic DNA. Translation: AAA40755.1.
S76779 mRNA. Translation: AAC60703.1.
BC086581 mRNA. Translation: AAH86581.1.
PIRiA26189. LPRTE.
RefSeqiNP_001257610.1. NM_001270681.1.
NP_001257611.1. NM_001270682.1.
NP_001257612.1. NM_001270683.1.
NP_001257613.1. NM_001270684.1.
NP_620183.2. NM_138828.3.
UniGeneiRn.32351.

3D structure databases

ProteinModelPortaliP02650.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247757. 1 interactor.
IntActiP02650. 3 interactors.
MINTiMINT-4585732.
STRINGi10116.ENSRNOP00000050968.

PTM databases

iPTMnetiP02650.
PhosphoSitePlusiP02650.

Proteomic databases

PaxDbiP02650.
PRIDEiP02650.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000041891; ENSRNOP00000050968; ENSRNOG00000018454.
ENSRNOT00000091574; ENSRNOP00000068937; ENSRNOG00000018454.
GeneIDi25728.
KEGGirno:25728.
UCSCiRGD:2138. rat.

Organism-specific databases

CTDi348.
RGDi2138. Apoe.

Phylogenomic databases

eggNOGiENOG410IVK0. Eukaryota.
ENOG4111MYC. LUCA.
GeneTreeiENSGT00730000111315.
HOGENOMiHOG000034006.
HOVERGENiHBG010582.
InParanoidiP02650.
KOiK04524.
PhylomeDBiP02650.
TreeFamiTF334458.

Enzyme and pathway databases

ReactomeiR-RNO-174800. Chylomicron-mediated lipid transport.
R-RNO-194223. HDL-mediated lipid transport.
R-RNO-3000480. Scavenging by Class A Receptors.
R-RNO-975634. Retinoid metabolism and transport.

Miscellaneous databases

PROiP02650.

Gene expression databases

BgeeiENSRNOG00000018454.
ExpressionAtlasiP02650. baseline and differential.
GenevisibleiP02650. RN.

Family and domain databases

InterProiIPR000074. ApoA_E.
[Graphical view]
PfamiPF01442. Apolipoprotein. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPOE_RAT
AccessioniPrimary (citable) accession number: P02650
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The mature protein has no cysteine residues; however, in different allelic variants where cysteine residues replace arginine at positions 155 or 168, binding of Apo-E to cell membrane receptors is decreased. The amino end of this protein is therefore thought to interact with the receptor.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.