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Protein

Apolipoprotein E

Gene

Apoe

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.By similarity

Miscellaneous

The mature protein has no cysteine residues; however, in different allelic variants where cysteine residues replace arginine at positions 155 or 168, binding of Apo-E to cell membrane receptors is decreased. The amino end of this protein is therefore thought to interact with the receptor.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHeparin-binding
Biological processLipid transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-3000480. Scavenging by Class A Receptors.
R-RNO-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
R-RNO-8957275. Post-translational protein phosphorylation.
R-RNO-8963888. Chylomicron assembly.
R-RNO-8963901. Chylomicron remodeling.
R-RNO-8964026. Chylomicron clearance.
R-RNO-8964058. HDL remodeling.
R-RNO-975634. Retinoid metabolism and transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Apolipoprotein E
Short name:
Apo-E
Gene namesi
Name:Apoe
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi2138. Apoe.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chylomicron, HDL, Secreted, VLDL

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Add BLAST18
ChainiPRO_000000199619 – 312Apolipoprotein EAdd BLAST294

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi25O-linked (GalNAc...) threonineBy similarity1
Modified residuei135Methionine sulfoxideBy similarity1
Modified residuei139PhosphoserineCombined sources1
Glycosylationi305O-linked (GalNAc...) threonineBy similarity1

Post-translational modificationi

Phosphorylated by FAM20C in the extracellular medium.By similarity

Keywords - PTMi

Glycoprotein, Oxidation, Phosphoprotein

Proteomic databases

PaxDbiP02650.
PRIDEiP02650.

PTM databases

iPTMnetiP02650.
PhosphoSitePlusiP02650.

Expressioni

Tissue specificityi

Secreted in plasma.

Gene expression databases

BgeeiENSRNOG00000018454.
ExpressionAtlasiP02650. baseline and differential.
GenevisibleiP02650. RN.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi247757. 2 interactors.
IntActiP02650. 3 interactors.
STRINGi10116.ENSRNOP00000050968.

Structurei

3D structure databases

ProteinModelPortaliP02650.
SMRiP02650.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati72 – 931Add BLAST22
Repeati94 – 1152Add BLAST22
Repeati116 – 1373Add BLAST22
Repeati138 – 1594Add BLAST22
Repeati160 – 1815Add BLAST22
Repeati182 – 2036Add BLAST22
Repeati204 – 2257Add BLAST22
Repeati226 – 2478Add BLAST22

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni72 – 2478 X 22 AA approximate tandem repeatsAdd BLAST176
Regioni150 – 160LDL receptor bindingBy similarityAdd BLAST11
Regioni154 – 157Heparin-bindingBy similarity4
Regioni221 – 228Heparin-bindingBy similarity8

Sequence similaritiesi

Belongs to the apolipoprotein A1/A4/E family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IVK0. Eukaryota.
ENOG4111MYC. LUCA.
GeneTreeiENSGT00730000111315.
HOGENOMiHOG000034006.
HOVERGENiHBG010582.
InParanoidiP02650.
KOiK04524.
PhylomeDBiP02650.
TreeFamiTF334458.

Family and domain databases

InterProiView protein in InterPro
IPR000074. ApoA_E.
PfamiView protein in Pfam
PF01442. Apolipoprotein. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02650-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKALWALLLV PLLTGCLAEG ELEVTDQLPG QSDQPWEQAL NRFWDYLRWV
60 70 80 90 100
QTLSDQVQEE LQSSQVTQEL TVLMEDTMTE VKAYKKELEE QLGPVAEETR
110 120 130 140 150
ARLAKEVQAA QARLGADMED LRNRLGQYRN EVNTMLGQST EELRSRLSTH
160 170 180 190 200
LRKMRKRLMR DADDLQKRLA VYKAGAQEGA ERGVSAIRER LGPLVEQGRQ
210 220 230 240 250
RTANLGAGAA QPLRDRAQAL SDRIRGRLEE VGNQARDRLE EVREQMEEVR
260 270 280 290 300
SKMEEQTQQI RLQAEIFQAR IKGWFEPLVE DMQRQWANLM EKIQASVATN
310
SIASTTVPLE NQ
Length:312
Mass (Da):35,753
Last modified:October 1, 1996 - v2
Checksum:i8180EEE933378D92
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti104A → T in CAA28650 (PubMed:3797247).Curated1
Sequence conflicti110A → T no nucleotide entry (PubMed:6190813).Curated1
Sequence conflicti110A → T in AAC60703 (PubMed:7766086).Curated1
Sequence conflicti141E → D no nucleotide entry (PubMed:6190813).Curated1
Sequence conflicti141E → D in AAC60703 (PubMed:7766086).Curated1
Sequence conflicti206 – 213GAGAAQPL → RWRRPAP in CAA28650 (PubMed:3797247).Curated8
Sequence conflicti206 – 213GAGAAQPL → RWRRPAP no nucleotide entry (PubMed:6190813).Curated8
Sequence conflicti309 – 310LE → WR no nucleotide entry (PubMed:6190813).Curated2
Sequence conflicti309 – 310LE → WR in AAC60703 (PubMed:7766086).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04979 Genomic DNA. Translation: CAA28650.1.
J02582 Genomic DNA. Translation: AAA40755.1.
S76779 mRNA. Translation: AAC60703.1.
BC086581 mRNA. Translation: AAH86581.1.
PIRiA26189. LPRTE.
RefSeqiNP_001257610.1. NM_001270681.1.
NP_001257611.1. NM_001270682.1.
NP_001257612.1. NM_001270683.1.
NP_001257613.1. NM_001270684.1.
NP_620183.2. NM_138828.3.
UniGeneiRn.32351.

Genome annotation databases

EnsembliENSRNOT00000041891; ENSRNOP00000050968; ENSRNOG00000018454.
ENSRNOT00000091574; ENSRNOP00000068937; ENSRNOG00000018454.
GeneIDi25728.
KEGGirno:25728.
UCSCiRGD:2138. rat.

Similar proteinsi

Entry informationi

Entry nameiAPOE_RAT
AccessioniPrimary (citable) accession number: P02650
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 1, 1996
Last modified: February 28, 2018
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome