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Protein

Apolipoprotein E

Gene

Apoe

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.By similarity

Miscellaneous

The mature protein has no cysteine residues; however, in different allelic variants where cysteine residues replace arginine at positions 155 or 168, binding of Apo-E to cell membrane receptors is decreased. The amino end of this protein is therefore thought to interact with the receptor.

GO - Molecular functioni

  • amyloid-beta binding Source: RGD
  • cholesterol binding Source: GO_Central
  • cholesterol transporter activity Source: GO_Central
  • heparin binding Source: UniProtKB-KW
  • hydroxyapatite binding Source: RGD
  • lipid transporter activity Source: RGD
  • low-density lipoprotein particle receptor binding Source: GO_Central
  • phosphatidylcholine-sterol O-acyltransferase activator activity Source: GO_Central
  • phospholipid binding Source: RGD
  • signaling receptor binding Source: UniProtKB
  • very-low-density lipoprotein particle receptor binding Source: GO_Central

GO - Biological processi

  • aging Source: RGD
  • cellular response to cholesterol Source: RGD
  • cellular response to ethanol Source: RGD
  • cellular response to growth factor stimulus Source: RGD
  • cellular response to interleukin-1 Source: RGD
  • cholesterol catabolic process Source: GO_Central
  • cholesterol efflux Source: GO_Central
  • cholesterol homeostasis Source: GO_Central
  • high-density lipoprotein particle assembly Source: GO_Central
  • lipid transport Source: RGD
  • lipoprotein catabolic process Source: GO_Central
  • lipoprotein metabolic process Source: RGD
  • negative regulation of cholesterol biosynthetic process Source: RGD
  • negative regulation of neuron apoptotic process Source: RGD
  • neuron projection regeneration Source: RGD
  • oligodendrocyte differentiation Source: RGD
  • peripheral nervous system axon regeneration Source: RGD
  • phospholipid efflux Source: GO_Central
  • positive regulation of axon extension Source: RGD
  • positive regulation of cholesterol esterification Source: GO_Central
  • positive regulation of lipid biosynthetic process Source: GO_Central
  • positive regulation of nitric-oxide synthase activity Source: GO_Central
  • regulation of amyloid-beta clearance Source: GO_Central
  • regulation of cholesterol transport Source: GO_Central
  • regulation of triglyceride metabolic process Source: RGD
  • response to D-galactosamine Source: RGD
  • response to ethanol Source: RGD
  • response to insulin Source: RGD
  • response to retinoic acid Source: RGD
  • response to tumor necrosis factor Source: RGD
  • response to zinc ion Source: RGD
  • reverse cholesterol transport Source: GO_Central
  • triglyceride catabolic process Source: GO_Central
  • very-low-density lipoprotein particle remodeling Source: GO_Central

Keywordsi

Molecular functionHeparin-binding
Biological processLipid transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-3000480 Scavenging by Class A Receptors
R-RNO-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-RNO-8957275 Post-translational protein phosphorylation
R-RNO-8963888 Chylomicron assembly
R-RNO-8963901 Chylomicron remodeling
R-RNO-8964026 Chylomicron clearance
R-RNO-8964058 HDL remodeling
R-RNO-975634 Retinoid metabolism and transport

Names & Taxonomyi

Protein namesi
Recommended name:
Apolipoprotein E
Short name:
Apo-E
Gene namesi
Name:Apoe
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi2138 Apoe

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chylomicron, HDL, Secreted, VLDL

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Add BLAST18
ChainiPRO_000000199619 – 312Apolipoprotein EAdd BLAST294

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi25O-linked (GalNAc...) threonineBy similarity1
Modified residuei135Methionine sulfoxideBy similarity1
Modified residuei139PhosphoserineCombined sources1
Glycosylationi305O-linked (GalNAc...) threonineBy similarity1

Post-translational modificationi

Phosphorylated by FAM20C in the extracellular medium.By similarity

Keywords - PTMi

Glycoprotein, Oxidation, Phosphoprotein

Proteomic databases

PaxDbiP02650
PRIDEiP02650

PTM databases

iPTMnetiP02650
PhosphoSitePlusiP02650

Expressioni

Tissue specificityi

Secreted in plasma.

Gene expression databases

BgeeiENSRNOG00000018454
ExpressionAtlasiP02650 baseline and differential
GenevisibleiP02650 RN

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi247757, 2 interactors
IntActiP02650, 3 interactors
STRINGi10116.ENSRNOP00000050968

Structurei

3D structure databases

ProteinModelPortaliP02650
SMRiP02650
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati72 – 931Add BLAST22
Repeati94 – 1152Add BLAST22
Repeati116 – 1373Add BLAST22
Repeati138 – 1594Add BLAST22
Repeati160 – 1815Add BLAST22
Repeati182 – 2036Add BLAST22
Repeati204 – 2257Add BLAST22
Repeati226 – 2478Add BLAST22

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni72 – 2478 X 22 AA approximate tandem repeatsAdd BLAST176
Regioni150 – 160LDL receptor bindingBy similarityAdd BLAST11
Regioni154 – 157Heparin-bindingBy similarity4
Regioni221 – 228Heparin-bindingBy similarity8

Sequence similaritiesi

Belongs to the apolipoprotein A1/A4/E family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IVK0 Eukaryota
ENOG4111MYC LUCA
GeneTreeiENSGT00730000111315
HOGENOMiHOG000034006
HOVERGENiHBG010582
InParanoidiP02650
KOiK04524
PhylomeDBiP02650
TreeFamiTF334458

Family and domain databases

InterProiView protein in InterPro
IPR000074 ApoA_E
PfamiView protein in Pfam
PF01442 Apolipoprotein, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02650-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKALWALLLV PLLTGCLAEG ELEVTDQLPG QSDQPWEQAL NRFWDYLRWV
60 70 80 90 100
QTLSDQVQEE LQSSQVTQEL TVLMEDTMTE VKAYKKELEE QLGPVAEETR
110 120 130 140 150
ARLAKEVQAA QARLGADMED LRNRLGQYRN EVNTMLGQST EELRSRLSTH
160 170 180 190 200
LRKMRKRLMR DADDLQKRLA VYKAGAQEGA ERGVSAIRER LGPLVEQGRQ
210 220 230 240 250
RTANLGAGAA QPLRDRAQAL SDRIRGRLEE VGNQARDRLE EVREQMEEVR
260 270 280 290 300
SKMEEQTQQI RLQAEIFQAR IKGWFEPLVE DMQRQWANLM EKIQASVATN
310
SIASTTVPLE NQ
Length:312
Mass (Da):35,753
Last modified:October 1, 1996 - v2
Checksum:i8180EEE933378D92
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti104A → T in CAA28650 (PubMed:3797247).Curated1
Sequence conflicti110A → T no nucleotide entry (PubMed:6190813).Curated1
Sequence conflicti110A → T in AAC60703 (PubMed:7766086).Curated1
Sequence conflicti141E → D no nucleotide entry (PubMed:6190813).Curated1
Sequence conflicti141E → D in AAC60703 (PubMed:7766086).Curated1
Sequence conflicti206 – 213GAGAAQPL → RWRRPAP in CAA28650 (PubMed:3797247).Curated8
Sequence conflicti206 – 213GAGAAQPL → RWRRPAP no nucleotide entry (PubMed:6190813).Curated8
Sequence conflicti309 – 310LE → WR no nucleotide entry (PubMed:6190813).Curated2
Sequence conflicti309 – 310LE → WR in AAC60703 (PubMed:7766086).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04979 Genomic DNA Translation: CAA28650.1
J02582 Genomic DNA Translation: AAA40755.1
S76779 mRNA Translation: AAC60703.1
BC086581 mRNA Translation: AAH86581.1
PIRiA26189 LPRTE
RefSeqiNP_001257610.1, NM_001270681.1
NP_001257611.1, NM_001270682.1
NP_001257612.1, NM_001270683.1
NP_001257613.1, NM_001270684.1
NP_620183.2, NM_138828.3
UniGeneiRn.32351

Genome annotation databases

EnsembliENSRNOT00000041891; ENSRNOP00000050968; ENSRNOG00000018454
ENSRNOT00000091574; ENSRNOP00000068937; ENSRNOG00000018454
GeneIDi25728
KEGGirno:25728
UCSCiRGD:2138 rat

Similar proteinsi

Entry informationi

Entry nameiAPOE_RAT
AccessioniPrimary (citable) accession number: P02650
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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