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P02650

- APOE_RAT

UniProt

P02650 - APOE_RAT

Protein

Apolipoprotein E

Gene

Apoe

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.

    GO - Molecular functioni

    1. antioxidant activity Source: Ensembl
    2. beta-amyloid binding Source: UniProtKB
    3. cholesterol transporter activity Source: Ensembl
    4. heparin binding Source: UniProtKB-KW
    5. hydroxyapatite binding Source: RGD
    6. lipid transporter activity Source: RGD
    7. lipoprotein particle binding Source: Ensembl
    8. metal chelating activity Source: Ensembl
    9. phosphatidylcholine-sterol O-acyltransferase activator activity Source: Ensembl
    10. phospholipid binding Source: RGD
    11. receptor binding Source: UniProt

    GO - Biological processi

    1. aging Source: RGD
    2. artery morphogenesis Source: Ensembl
    3. cellular calcium ion homeostasis Source: Ensembl
    4. cellular response to cholesterol Source: RGD
    5. cellular response to growth factor stimulus Source: RGD
    6. cellular response to interleukin-1 Source: RGD
    7. cGMP-mediated signaling Source: Ensembl
    8. cholesterol catabolic process Source: Ensembl
    9. cholesterol efflux Source: Ensembl
    10. cholesterol homeostasis Source: Ensembl
    11. chylomicron remnant clearance Source: Ensembl
    12. G-protein coupled receptor signaling pathway Source: Ensembl
    13. high-density lipoprotein particle assembly Source: Ensembl
    14. high-density lipoprotein particle clearance Source: Ensembl
    15. high-density lipoprotein particle remodeling Source: Ensembl
    16. lipid transport Source: RGD
    17. lipoprotein biosynthetic process Source: Ensembl
    18. lipoprotein catabolic process Source: Ensembl
    19. lipoprotein metabolic process Source: RGD
    20. low-density lipoprotein particle remodeling Source: Ensembl
    21. maintenance of location in cell Source: Ensembl
    22. negative regulation of blood vessel endothelial cell migration Source: Ensembl
    23. negative regulation of cholesterol biosynthetic process Source: Ensembl
    24. negative regulation of endothelial cell proliferation Source: Ensembl
    25. negative regulation of inflammatory response Source: Ensembl
    26. negative regulation of MAP kinase activity Source: Ensembl
    27. negative regulation of neuron apoptotic process Source: RGD
    28. negative regulation of platelet activation Source: Ensembl
    29. neuron projection regeneration Source: RGD
    30. nitric oxide mediated signal transduction Source: Ensembl
    31. oligodendrocyte differentiation Source: RGD
    32. peripheral nervous system axon regeneration Source: RGD
    33. phospholipid efflux Source: Ensembl
    34. positive regulation of axon extension Source: RGD
    35. positive regulation of cGMP biosynthetic process Source: Ensembl
    36. positive regulation of cholesterol efflux Source: Ensembl
    37. positive regulation of cholesterol esterification Source: Ensembl
    38. positive regulation of low-density lipoprotein particle receptor catabolic process Source: Ensembl
    39. positive regulation of membrane protein ectodomain proteolysis Source: Ensembl
    40. positive regulation of nitric-oxide synthase activity Source: Ensembl
    41. receptor-mediated endocytosis Source: Ensembl
    42. regulation of Cdc42 protein signal transduction Source: Ensembl
    43. response to dietary excess Source: Ensembl
    44. response to ethanol Source: RGD
    45. response to insulin Source: RGD
    46. response to oxidative stress Source: Ensembl
    47. response to retinoic acid Source: RGD
    48. reverse cholesterol transport Source: Ensembl
    49. triglyceride metabolic process Source: Ensembl
    50. vasodilation Source: Ensembl
    51. very-low-density lipoprotein particle clearance Source: Ensembl
    52. very-low-density lipoprotein particle remodeling Source: Ensembl

    Keywords - Biological processi

    Lipid transport, Transport

    Keywords - Ligandi

    Heparin-binding

    Enzyme and pathway databases

    ReactomeiREACT_198733. Scavenging by Class A Receptors.
    REACT_199015. Retinoid metabolism and transport.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Apolipoprotein E
    Short name:
    Apo-E
    Gene namesi
    Name:Apoe
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi2138. Apoe.

    Subcellular locationi

    GO - Cellular componenti

    1. cell surface Source: RGD
    2. chylomicron Source: UniProtKB-KW
    3. cytoplasm Source: RGD
    4. dendrite Source: RGD
    5. discoidal high-density lipoprotein particle Source: RGD
    6. endoplasmic reticulum Source: RGD
    7. endosome Source: RGD
    8. extracellular space Source: RGD
    9. extrinsic component of external side of plasma membrane Source: RGD
    10. Golgi apparatus Source: RGD
    11. intermediate-density lipoprotein particle Source: Ensembl
    12. late endosome Source: RGD
    13. low-density lipoprotein particle Source: Ensembl
    14. lysosome Source: RGD
    15. neuronal cell body Source: RGD
    16. nuclear envelope Source: RGD
    17. plasma membrane Source: RGD
    18. very-low-density lipoprotein particle Source: UniProtKB-KW

    Keywords - Cellular componenti

    Chylomicron, HDL, Secreted, VLDL

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Add
    BLAST
    Chaini19 – 312294Apolipoprotein EPRO_0000001996Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei135 – 1351Methionine sulfoxideBy similarity

    Post-translational modificationi

    Phosphorylation sites are present in the extracellular medium.By similarity

    Keywords - PTMi

    Oxidation, Phosphoprotein

    Proteomic databases

    PaxDbiP02650.
    PRIDEiP02650.

    PTM databases

    PhosphoSiteiP02650.

    Expressioni

    Tissue specificityi

    Secreted in plasma.

    Gene expression databases

    GenevestigatoriP02650.

    Interactioni

    Protein-protein interaction databases

    BioGridi247757. 1 interaction.
    IntActiP02650. 3 interactions.
    MINTiMINT-4585732.
    STRINGi10116.ENSRNOP00000050968.

    Structurei

    3D structure databases

    ProteinModelPortaliP02650.
    SMRiP02650. Positions 23-195.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati72 – 93221Add
    BLAST
    Repeati94 – 115222Add
    BLAST
    Repeati116 – 137223Add
    BLAST
    Repeati138 – 159224Add
    BLAST
    Repeati160 – 181225Add
    BLAST
    Repeati182 – 203226Add
    BLAST
    Repeati204 – 225227Add
    BLAST
    Repeati226 – 247228Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni72 – 2471768 X 22 AA approximate tandem repeatsAdd
    BLAST
    Regioni150 – 16011LDL receptor bindingSequence AnalysisAdd
    BLAST
    Regioni154 – 1574Heparin-bindingBy similarity
    Regioni221 – 2288Heparin-bindingBy similarity

    Sequence similaritiesi

    Belongs to the apolipoprotein A1/A4/E family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG44867.
    GeneTreeiENSGT00730000111315.
    HOGENOMiHOG000034006.
    HOVERGENiHBG010582.
    InParanoidiP02650.
    KOiK04524.
    OMAiPLQERAQ.
    OrthoDBiEOG793B87.
    PhylomeDBiP02650.
    TreeFamiTF334458.

    Family and domain databases

    InterProiIPR000074. ApoA1_A4_E.
    [Graphical view]
    PfamiPF01442. Apolipoprotein. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P02650-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKALWALLLV PLLTGCLAEG ELEVTDQLPG QSDQPWEQAL NRFWDYLRWV    50
    QTLSDQVQEE LQSSQVTQEL TVLMEDTMTE VKAYKKELEE QLGPVAEETR 100
    ARLAKEVQAA QARLGADMED LRNRLGQYRN EVNTMLGQST EELRSRLSTH 150
    LRKMRKRLMR DADDLQKRLA VYKAGAQEGA ERGVSAIRER LGPLVEQGRQ 200
    RTANLGAGAA QPLRDRAQAL SDRIRGRLEE VGNQARDRLE EVREQMEEVR 250
    SKMEEQTQQI RLQAEIFQAR IKGWFEPLVE DMQRQWANLM EKIQASVATN 300
    SIASTTVPLE NQ 312
    Length:312
    Mass (Da):35,753
    Last modified:October 1, 1996 - v2
    Checksum:i8180EEE933378D92
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti104 – 1041A → T in CAA28650. (PubMed:3797247)Curated
    Sequence conflicti110 – 1101A → T no nucleotide entry (PubMed:6190813)Curated
    Sequence conflicti110 – 1101A → T in AAC60703. (PubMed:7766086)Curated
    Sequence conflicti141 – 1411E → D no nucleotide entry (PubMed:6190813)Curated
    Sequence conflicti141 – 1411E → D in AAC60703. (PubMed:7766086)Curated
    Sequence conflicti206 – 2138GAGAAQPL → RWRRPAP in CAA28650. (PubMed:3797247)Curated
    Sequence conflicti206 – 2138GAGAAQPL → RWRRPAP no nucleotide entry (PubMed:6190813)Curated
    Sequence conflicti309 – 3102LE → WR no nucleotide entry (PubMed:6190813)Curated
    Sequence conflicti309 – 3102LE → WR in AAC60703. (PubMed:7766086)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04979 Genomic DNA. Translation: CAA28650.1.
    J02582 Genomic DNA. Translation: AAA40755.1.
    S76779 mRNA. Translation: AAC60703.1.
    BC086581 mRNA. Translation: AAH86581.1.
    PIRiA26189. LPRTE.
    RefSeqiNP_001257610.1. NM_001270681.1.
    NP_001257611.1. NM_001270682.1.
    NP_001257612.1. NM_001270683.1.
    NP_001257613.1. NM_001270684.1.
    NP_620183.2. NM_138828.3.
    UniGeneiRn.32351.

    Genome annotation databases

    EnsembliENSRNOT00000041891; ENSRNOP00000050968; ENSRNOG00000018454.
    GeneIDi25728.
    KEGGirno:25728.
    UCSCiRGD:2138. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04979 Genomic DNA. Translation: CAA28650.1 .
    J02582 Genomic DNA. Translation: AAA40755.1 .
    S76779 mRNA. Translation: AAC60703.1 .
    BC086581 mRNA. Translation: AAH86581.1 .
    PIRi A26189. LPRTE.
    RefSeqi NP_001257610.1. NM_001270681.1.
    NP_001257611.1. NM_001270682.1.
    NP_001257612.1. NM_001270683.1.
    NP_001257613.1. NM_001270684.1.
    NP_620183.2. NM_138828.3.
    UniGenei Rn.32351.

    3D structure databases

    ProteinModelPortali P02650.
    SMRi P02650. Positions 23-195.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 247757. 1 interaction.
    IntActi P02650. 3 interactions.
    MINTi MINT-4585732.
    STRINGi 10116.ENSRNOP00000050968.

    PTM databases

    PhosphoSitei P02650.

    Proteomic databases

    PaxDbi P02650.
    PRIDEi P02650.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000041891 ; ENSRNOP00000050968 ; ENSRNOG00000018454 .
    GeneIDi 25728.
    KEGGi rno:25728.
    UCSCi RGD:2138. rat.

    Organism-specific databases

    CTDi 348.
    RGDi 2138. Apoe.

    Phylogenomic databases

    eggNOGi NOG44867.
    GeneTreei ENSGT00730000111315.
    HOGENOMi HOG000034006.
    HOVERGENi HBG010582.
    InParanoidi P02650.
    KOi K04524.
    OMAi PLQERAQ.
    OrthoDBi EOG793B87.
    PhylomeDBi P02650.
    TreeFami TF334458.

    Enzyme and pathway databases

    Reactomei REACT_198733. Scavenging by Class A Receptors.
    REACT_199015. Retinoid metabolism and transport.

    Miscellaneous databases

    NextBioi 607845.
    PROi P02650.

    Gene expression databases

    Genevestigatori P02650.

    Family and domain databases

    InterProi IPR000074. ApoA1_A4_E.
    [Graphical view ]
    Pfami PF01442. Apolipoprotein. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of the gene encoding the rat apolipoprotein E."
      Fukazawa C., Matsumoto A., Taylor L.M.
      Nucleic Acids Res. 14:9527-9528(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Structure and expression of the rat apolipoprotein E gene."
      Fung W.-P., Howlett G.J., Schreiber G.
      J. Biol. Chem. 261:13777-13783(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Rat apolipoprotein E mRNA. Cloning and sequencing of double-stranded cDNA."
      McLean J.W., Fukazawa C., Taylor J.M.
      J. Biol. Chem. 258:8993-9000(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "Secretion by Saccharomyces cerevisiae of rat apolipoprotein E as a fusion to Mucor rennin."
      Nomura N., Yamada H., Matsubara N., Horinouchi S., Beppu T.
      Appl. Microbiol. Biotechnol. 42:865-870(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Liver.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Ovary.
    6. Lubec G., Chen W.-Q.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 87-100; 114-122; 130-144; 191-199; 202-216; 226-236 AND 262-270, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus.

    Entry informationi

    Entry nameiAPOE_RAT
    AccessioniPrimary (citable) accession number: P02650
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The mature protein has no cysteine residues; however, in different allelic variants where cysteine residues replace arginine at positions 155 or 168, binding of Apo-E to cell membrane receptors is decreased. The amino end of this protein is therefore thought to interact with the receptor.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3