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Protein

Apolipoprotein A-I

Gene

APOA1

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cholesterol metabolism, Lipid metabolism, Lipid transport, Steroid metabolism, Sterol metabolism, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Apolipoprotein A-I
Short name:
Apo-AI
Short name:
ApoA-I
Alternative name(s):
Apolipoprotein A1
Cleaved into the following 2 chains:
Gene namesi
Name:APOA1
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

HDL, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 181 PublicationAdd BLAST18
ChainiPRO_000042532019 – 266Proapolipoprotein A-IAdd BLAST248
ChainiPRO_000000193725 – 266Apolipoprotein A-IAdd BLAST242
ChainiPRO_000041657225 – 265Truncated apolipoprotein A-IBy similarityAdd BLAST241

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei109Methionine sulfoxide1 Publication1

Post-translational modificationi

Palmitoylated.1 Publication
Glycosylated.1 Publication
Phosphorylation sites are present in the extracellular medium.By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Oxidation, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP02648.
PRIDEiP02648.

2D gel databases

UCD-2DPAGEP02648.

Expressioni

Tissue specificityi

Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.

Interactioni

Subunit structurei

Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000019630.

Structurei

3D structure databases

ProteinModelPortaliP02648.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati67 – 881Add BLAST22
Repeati89 – 1102Add BLAST22
Repeati111 – 1213; half-lengthAdd BLAST11
Repeati122 – 1434Add BLAST22
Repeati144 – 1655Add BLAST22
Repeati166 – 1876Add BLAST22
Repeati188 – 2097Add BLAST22
Repeati210 – 2318Add BLAST22
Repeati232 – 2429; half-lengthAdd BLAST11
Repeati243 – 26610Add BLAST24

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni67 – 26610 X approximate tandem repeatsAdd BLAST200

Sequence similaritiesi

Belongs to the apolipoprotein A1/A4/E family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IWKR. Eukaryota.
ENOG410YGQ6. LUCA.
HOGENOMiHOG000033998.
HOVERGENiHBG105708.
InParanoidiP02648.

Family and domain databases

InterProiIPR000074. ApoA_E.
[Graphical view]
PfamiPF01442. Apolipoprotein. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02648-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAALLTLAV LFLTGSQARH FWQQDEPQSP WDRVKDLATV YVDAVKDSGR
60 70 80 90 100
DYVAQFEASA LGKQLNLKLL DNWDSLSSTV TKLREQIGPV TQEFWDNLEK
110 120 130 140 150
ETEVLRQEMS KDLEEVKQKV QPYLDDFQKK WQEEVELYRQ KVAPLGSELR
160 170 180 190 200
EGARQKLQEL QEKLSPLAEE LRDRARTHVD ALRAQLAPYS DDLRERLAAR
210 220 230 240 250
LEALKEGGGA SLAEYHARAS EQLSALGEKA RPALEDLRQG LLPVLESFKV
260
SLLAAIDEAT KKLNAQ
Length:266
Mass (Da):30,196
Last modified:December 15, 1998 - v2
Checksum:iA3202620C28A869D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti168A → G AA sequence (PubMed:20483223).Curated1
Sequence conflicti168A → G AA sequence (PubMed:6801039).Curated1
Sequence conflicti202E → Q AA sequence (PubMed:20483223).Curated1
Sequence conflicti202E → Q AA sequence (PubMed:6801039).Curated1
Sequence conflicti235E → Q AA sequence (PubMed:6801039).Curated1
Sequence conflicti264 – 266NAQ → A AA sequence (PubMed:179887).Curated3

Mass spectrometryi

Molecular mass is 27468.3±0.071 Da from positions 25 - 266. Determined by ESI. 1 Publication
Molecular mass is 28354 Da from positions 19 - 266. Determined by ESI. 1 Publication
Molecular mass is 27470 Da from positions 25 - 266. Determined by ESI. Glycosylated ApoA-I.1 Publication
Molecular mass is 27733±0.071 Da from positions 25 - 266. Determined by ESI. Stearoylated ApoA-I.1 Publication
Molecular mass is 27707 Da from positions 25 - 266. Determined by ESI. Palmitoylated ApoA-I.1 Publication

Sequence databases

PIRiA60940. LPDGA1.

Cross-referencesi

Sequence databases

PIRiA60940. LPDGA1.

3D structure databases

ProteinModelPortaliP02648.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000019630.

2D gel databases

UCD-2DPAGEP02648.

Proteomic databases

PaxDbiP02648.
PRIDEiP02648.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410IWKR. Eukaryota.
ENOG410YGQ6. LUCA.
HOGENOMiHOG000033998.
HOVERGENiHBG105708.
InParanoidiP02648.

Family and domain databases

InterProiIPR000074. ApoA_E.
[Graphical view]
PfamiPF01442. Apolipoprotein. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPOA1_CANLF
AccessioniPrimary (citable) accession number: P02648
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: December 15, 1998
Last modified: February 17, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.