P02648 (APOA1_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Apolipoprotein A-I
Short name=Apo-AI
Short name=ApoA-I

Alternative name(s):
Apolipoprotein A1

Cleaved into the following chain:

1. Truncated apolipoprotein A-I

Gene names

Name:

APOA1

Organism

Canis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › Canis lupus

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Subunit structure	Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1 By similarity.
Subcellular location	Secreted.
Tissue specificity	Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.
Post-translational modification	Palmitoylated By similarity. Met-109 is oxidized to methionine sulfoxide By similarity. Phosphorylation sites are present in the extracellular medium By similarity.
Sequence similarities	Belongs to the apolipoprotein A1/A4/E family.

Ontologies

Keywords
Biological process	Cholesterol metabolism Lipid metabolism Lipid transport Steroid metabolism Sterol metabolism Transport
Cellular component	HDL Secreted
Domain	Repeat Signal
PTM	Lipoprotein Oxidation Palmitate Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cholesterol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW lipid transport Inferred from electronic annotation. Source: UniProtKB-KW lipoprotein metabolic process Inferred from electronic annotation. Source: InterPro peptidyl-methionine modification Inferred from sequence or structural similarity. Source: UniProtKB protein oxidation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	high-density lipoprotein particle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	lipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	By similarity
	Propeptide	19 – 24	6			PRO_0000001936
	Chain	25 – 266	242	Apolipoprotein A-I		PRO_0000001937
	Chain	25 – 265	241	Truncated apolipoprotein A-I By similarity		PRO_0000416572
Regions
	Repeat	67 – 88	22	1
	Repeat	89 – 110	22	2
	Repeat	111 – 121	11	3; half-length
	Repeat	122 – 143	22	4
	Repeat	144 – 165	22	5
	Repeat	166 – 187	22	6
	Repeat	188 – 209	22	7
	Repeat	210 – 231	22	8
	Repeat	232 – 242	11	9; half-length
	Repeat	243 – 266	24	10
	Region	67 – 266	200	10 X approximate tandem repeats
Amino acid modifications
	Modified residue	109	1	Methionine sulfoxide By similarity
Experimental info
	Sequence conflict	168	1	A → G AA sequence Ref.2
	Sequence conflict	202	1	E → Q AA sequence Ref.2
	Sequence conflict	235	1	E → Q AA sequence Ref.2
	Sequence conflict	264 – 266	3	NAQ → A AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P02648 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: A3202620C28A869D	FASTA	266	30,196
	10 20 30 40 50 60 MKAALLTLAV LFLTGSQARH FWQQDEPQSP WDRVKDLATV YVDAVKDSGR DYVAQFEASA 70 80 90 100 110 120 LGKQLNLKLL DNWDSLSSTV TKLREQIGPV TQEFWDNLEK ETEVLRQEMS KDLEEVKQKV 130 140 150 160 170 180 QPYLDDFQKK WQEEVELYRQ KVAPLGSELR EGARQKLQEL QEKLSPLAEE LRDRARTHVD 190 200 210 220 230 240 ALRAQLAPYS DDLRERLAAR LEALKEGGGA SLAEYHARAS EQLSALGEKA RPALEDLRQG 250 260 LLPVLESFKV SLLAAIDEAT KKLNAQ

References

[1]	"Structure and expression of dog apolipoprotein A-I, E, and C-I mRNAs: implications for the evolution and functional constraints of apolipoprotein structure." Luo C.-C., Li W.-H., Chan L. J. Lipid Res. 30:1735-1746(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"The covalent structure of apolipoprotein A-I from canine high density lipoproteins." Chung H., Randolph A., Reardon I., Heinrikson R.L. J. Biol. Chem. 257:2961-2967(1982) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-266.
[3]	"The amino- and carboxyl-terminal sequences of canine apolipoprotein A-i." Nakai T., Whayne T.F., Tang J. FEBS Lett. 64:409-411(1976) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-57 AND 262-265.
[4]	"HSC-2DPAGE and the two-dimensional gel electrophoresis database of dog heart proteins." Dunn M.J., Corbett J.M., Wheeler C.H. Electrophoresis 18:2795-2802(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-37. Tissue: Heart.

Cross-references

Sequence databases
PIR	LPDGA1. A60940.
3D structure databases
ProteinModelPortal	P02648.
SMR	P02648. Positions 25-266.
ModBase	Search...
Protein-protein interaction databases
STRING	9615.ENSCAFP00000019630.
2D gel databases
UCD-2DPAGE	P02648.
Proteomic databases
PaxDb	P02648.
PRIDE	P02648.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	NOG39720.
HOGENOM	HOG000033998.
HOVERGEN	HBG105708.
InParanoid	P02648.
OrthoDB	EOG44J2JV.
Family and domain databases
InterPro	IPR013326. ApoA. IPR000074. ApoA1_A4_E. [Graphical view]
Pfam	PF01442. Apolipoprotein. 1 hit. [Graphical view]
SUPFAM	SSF47162. SSF47162. 2 hits.
ProtoNet	Search...

Entry information

Entry name

APOA1_CANFA

Accession

Primary (citable) accession number: P02648

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	December 15, 1998
Last modified:	April 3, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program