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P02648

- APOA1_CANFA

UniProt

P02648 - APOA1_CANFA

Protein

Apolipoprotein A-I

Gene

APOA1

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 2 (15 Dec 1998)
      Previous versions | rss
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    Functioni

    Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

    GO - Molecular functioni

    1. lipid binding Source: InterPro

    GO - Biological processi

    1. cholesterol metabolic process Source: UniProtKB-KW
    2. lipid transport Source: UniProtKB-KW
    3. lipoprotein metabolic process Source: InterPro
    4. peptidyl-methionine modification Source: UniProtKB
    5. protein oxidation Source: UniProtKB

    Keywords - Biological processi

    Cholesterol metabolism, Lipid metabolism, Lipid transport, Steroid metabolism, Sterol metabolism, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Apolipoprotein A-I
    Short name:
    Apo-AI
    Short name:
    ApoA-I
    Alternative name(s):
    Apolipoprotein A1
    Cleaved into the following 2 chains:
    Gene namesi
    Name:APOA1
    OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
    Taxonomic identifieri9615 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
    ProteomesiUP000002254: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. high-density lipoprotein particle Source: UniProtKB-KW

    Keywords - Cellular componenti

    HDL, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 18181 PublicationAdd
    BLAST
    Chaini19 – 266248Proapolipoprotein A-IPRO_0000425320Add
    BLAST
    Chaini25 – 266242Apolipoprotein A-IPRO_0000001937Add
    BLAST
    Chaini25 – 265241Truncated apolipoprotein A-IBy similarityPRO_0000416572Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei109 – 1091Methionine sulfoxide1 Publication

    Post-translational modificationi

    Palmitoylated.1 Publication
    Glycosylated.1 Publication
    Phosphorylation sites are present in the extracellular medium.By similarity

    Keywords - PTMi

    Glycoprotein, Lipoprotein, Oxidation, Palmitate, Phosphoprotein

    Proteomic databases

    PaxDbiP02648.
    PRIDEiP02648.

    2D gel databases

    UCD-2DPAGEP02648.

    Expressioni

    Tissue specificityi

    Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.

    Interactioni

    Subunit structurei

    Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9615.ENSCAFP00000019630.

    Structurei

    3D structure databases

    ProteinModelPortaliP02648.
    SMRiP02648. Positions 25-266.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati67 – 88221Add
    BLAST
    Repeati89 – 110222Add
    BLAST
    Repeati111 – 121113; half-lengthAdd
    BLAST
    Repeati122 – 143224Add
    BLAST
    Repeati144 – 165225Add
    BLAST
    Repeati166 – 187226Add
    BLAST
    Repeati188 – 209227Add
    BLAST
    Repeati210 – 231228Add
    BLAST
    Repeati232 – 242119; half-lengthAdd
    BLAST
    Repeati243 – 2662410Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni67 – 26620010 X approximate tandem repeatsAdd
    BLAST

    Sequence similaritiesi

    Belongs to the apolipoprotein A1/A4/E family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG39720.
    HOGENOMiHOG000033998.
    HOVERGENiHBG105708.
    InParanoidiP02648.

    Family and domain databases

    InterProiIPR000074. ApoA1_A4_E.
    [Graphical view]
    PfamiPF01442. Apolipoprotein. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P02648-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKAALLTLAV LFLTGSQARH FWQQDEPQSP WDRVKDLATV YVDAVKDSGR    50
    DYVAQFEASA LGKQLNLKLL DNWDSLSSTV TKLREQIGPV TQEFWDNLEK 100
    ETEVLRQEMS KDLEEVKQKV QPYLDDFQKK WQEEVELYRQ KVAPLGSELR 150
    EGARQKLQEL QEKLSPLAEE LRDRARTHVD ALRAQLAPYS DDLRERLAAR 200
    LEALKEGGGA SLAEYHARAS EQLSALGEKA RPALEDLRQG LLPVLESFKV 250
    SLLAAIDEAT KKLNAQ 266
    Length:266
    Mass (Da):30,196
    Last modified:December 15, 1998 - v2
    Checksum:iA3202620C28A869D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti168 – 1681A → G AA sequence (PubMed:20483223)Curated
    Sequence conflicti168 – 1681A → G AA sequence (PubMed:6801039)Curated
    Sequence conflicti202 – 2021E → Q AA sequence (PubMed:20483223)Curated
    Sequence conflicti202 – 2021E → Q AA sequence (PubMed:6801039)Curated
    Sequence conflicti235 – 2351E → Q AA sequence (PubMed:6801039)Curated
    Sequence conflicti264 – 2663NAQ → A AA sequence (PubMed:179887)Curated

    Mass spectrometryi

    Molecular mass is 27468.3±0.071 Da from positions 25 - 266. Determined by ESI. 1 Publication
    Molecular mass is 28354 Da from positions 19 - 266. Determined by ESI. 1 Publication
    Molecular mass is 27470 Da from positions 25 - 266. Determined by ESI. Glycosylated ApoA-I.1 Publication
    Molecular mass is 27733±0.071 Da from positions 25 - 266. Determined by ESI. Stearoylated ApoA-I.1 Publication
    Molecular mass is 27707 Da from positions 25 - 266. Determined by ESI. Palmitoylated ApoA-I.1 Publication

    Sequence databases

    PIRiA60940. LPDGA1.

    Cross-referencesi

    Sequence databases

    PIRi A60940. LPDGA1.

    3D structure databases

    ProteinModelPortali P02648.
    SMRi P02648. Positions 25-266.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9615.ENSCAFP00000019630.

    2D gel databases

    UCD-2DPAGE P02648.

    Proteomic databases

    PaxDbi P02648.
    PRIDEi P02648.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi NOG39720.
    HOGENOMi HOG000033998.
    HOVERGENi HBG105708.
    InParanoidi P02648.

    Family and domain databases

    InterProi IPR000074. ApoA1_A4_E.
    [Graphical view ]
    Pfami PF01442. Apolipoprotein. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and expression of dog apolipoprotein A-I, E, and C-I mRNAs: implications for the evolution and functional constraints of apolipoprotein structure."
      Luo C.-C., Li W.-H., Chan L.
      J. Lipid Res. 30:1735-1746(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. "Mass spectral analysis of the apolipoproteins on dog (Canis lupus familiaris) high density lipoproteins. Detection of apolipoprotein A-II."
      Puppione D.L., Bassilian S., Souda P., MacDonald M.H., Halgand F., Hagland F., Whitelegge J.P.
      Comp. Biochem. Physiol. 3:290-296(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 19-139; 142-150; 155-172; 184-196 AND 201-266, MASS SPECTROMETRY, OXIDATION AT MET-109, GLYCOSYLATION, PALMITOYLATION.
      Tissue: Plasma.
    3. "The covalent structure of apolipoprotein A-I from canine high density lipoproteins."
      Chung H., Randolph A., Reardon I., Heinrikson R.L.
      J. Biol. Chem. 257:2961-2967(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-266.
    4. "The amino- and carboxyl-terminal sequences of canine apolipoprotein A-i."
      Nakai T., Whayne T.F., Tang J.
      FEBS Lett. 64:409-411(1976) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-57 AND 262-265.
    5. "HSC-2DPAGE and the two-dimensional gel electrophoresis database of dog heart proteins."
      Dunn M.J., Corbett J.M., Wheeler C.H.
      Electrophoresis 18:2795-2802(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-37.
      Tissue: Heart.

    Entry informationi

    Entry nameiAPOA1_CANFA
    AccessioniPrimary (citable) accession number: P02648
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 102 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3