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P02648

- APOA1_CANFA

UniProt

P02648 - APOA1_CANFA

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Protein

Apolipoprotein A-I

Gene

APOA1

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

GO - Molecular functioni

  1. lipid binding Source: InterPro

GO - Biological processi

  1. cholesterol metabolic process Source: UniProtKB-KW
  2. lipid transport Source: UniProtKB-KW
  3. lipoprotein metabolic process Source: InterPro
  4. peptidyl-methionine modification Source: UniProtKB
  5. protein oxidation Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cholesterol metabolism, Lipid metabolism, Lipid transport, Steroid metabolism, Sterol metabolism, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Apolipoprotein A-I
Short name:
Apo-AI
Short name:
ApoA-I
Alternative name(s):
Apolipoprotein A1
Cleaved into the following 2 chains:
Gene namesi
Name:APOA1
OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
ProteomesiUP000002254: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. high-density lipoprotein particle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

HDL, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 18181 PublicationAdd
BLAST
Chaini19 – 266248Proapolipoprotein A-IPRO_0000425320Add
BLAST
Chaini25 – 266242Apolipoprotein A-IPRO_0000001937Add
BLAST
Chaini25 – 265241Truncated apolipoprotein A-IBy similarityPRO_0000416572Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei109 – 1091Methionine sulfoxide1 Publication

Post-translational modificationi

Palmitoylated.1 Publication
Glycosylated.1 Publication
Phosphorylation sites are present in the extracellular medium.By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Oxidation, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP02648.
PRIDEiP02648.

2D gel databases

UCD-2DPAGEP02648.

Expressioni

Tissue specificityi

Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.

Interactioni

Subunit structurei

Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000019630.

Structurei

3D structure databases

ProteinModelPortaliP02648.
SMRiP02648. Positions 25-266.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati67 – 88221Add
BLAST
Repeati89 – 110222Add
BLAST
Repeati111 – 121113; half-lengthAdd
BLAST
Repeati122 – 143224Add
BLAST
Repeati144 – 165225Add
BLAST
Repeati166 – 187226Add
BLAST
Repeati188 – 209227Add
BLAST
Repeati210 – 231228Add
BLAST
Repeati232 – 242119; half-lengthAdd
BLAST
Repeati243 – 2662410Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni67 – 26620010 X approximate tandem repeatsAdd
BLAST

Sequence similaritiesi

Belongs to the apolipoprotein A1/A4/E family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG39720.
HOGENOMiHOG000033998.
HOVERGENiHBG105708.
InParanoidiP02648.

Family and domain databases

InterProiIPR000074. ApoA1_A4_E.
[Graphical view]
PfamiPF01442. Apolipoprotein. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02648-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKAALLTLAV LFLTGSQARH FWQQDEPQSP WDRVKDLATV YVDAVKDSGR
60 70 80 90 100
DYVAQFEASA LGKQLNLKLL DNWDSLSSTV TKLREQIGPV TQEFWDNLEK
110 120 130 140 150
ETEVLRQEMS KDLEEVKQKV QPYLDDFQKK WQEEVELYRQ KVAPLGSELR
160 170 180 190 200
EGARQKLQEL QEKLSPLAEE LRDRARTHVD ALRAQLAPYS DDLRERLAAR
210 220 230 240 250
LEALKEGGGA SLAEYHARAS EQLSALGEKA RPALEDLRQG LLPVLESFKV
260
SLLAAIDEAT KKLNAQ
Length:266
Mass (Da):30,196
Last modified:December 15, 1998 - v2
Checksum:iA3202620C28A869D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti168 – 1681A → G AA sequence (PubMed:20483223)Curated
Sequence conflicti168 – 1681A → G AA sequence (PubMed:6801039)Curated
Sequence conflicti202 – 2021E → Q AA sequence (PubMed:20483223)Curated
Sequence conflicti202 – 2021E → Q AA sequence (PubMed:6801039)Curated
Sequence conflicti235 – 2351E → Q AA sequence (PubMed:6801039)Curated
Sequence conflicti264 – 2663NAQ → A AA sequence (PubMed:179887)Curated

Mass spectrometryi

Molecular mass is 27468.3±0.071 Da from positions 25 - 266. Determined by ESI. 1 Publication
Molecular mass is 28354 Da from positions 19 - 266. Determined by ESI. 1 Publication
Molecular mass is 27470 Da from positions 25 - 266. Determined by ESI. Glycosylated ApoA-I.1 Publication
Molecular mass is 27733±0.071 Da from positions 25 - 266. Determined by ESI. Stearoylated ApoA-I.1 Publication
Molecular mass is 27707 Da from positions 25 - 266. Determined by ESI. Palmitoylated ApoA-I.1 Publication

Sequence databases

PIRiA60940. LPDGA1.

Cross-referencesi

Sequence databases

PIRi A60940. LPDGA1.

3D structure databases

ProteinModelPortali P02648.
SMRi P02648. Positions 25-266.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9615.ENSCAFP00000019630.

2D gel databases

UCD-2DPAGE P02648.

Proteomic databases

PaxDbi P02648.
PRIDEi P02648.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi NOG39720.
HOGENOMi HOG000033998.
HOVERGENi HBG105708.
InParanoidi P02648.

Family and domain databases

InterProi IPR000074. ApoA1_A4_E.
[Graphical view ]
Pfami PF01442. Apolipoprotein. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structure and expression of dog apolipoprotein A-I, E, and C-I mRNAs: implications for the evolution and functional constraints of apolipoprotein structure."
    Luo C.-C., Li W.-H., Chan L.
    J. Lipid Res. 30:1735-1746(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Mass spectral analysis of the apolipoproteins on dog (Canis lupus familiaris) high density lipoproteins. Detection of apolipoprotein A-II."
    Puppione D.L., Bassilian S., Souda P., MacDonald M.H., Halgand F., Hagland F., Whitelegge J.P.
    Comp. Biochem. Physiol. 3:290-296(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 19-139; 142-150; 155-172; 184-196 AND 201-266, MASS SPECTROMETRY, OXIDATION AT MET-109, GLYCOSYLATION, PALMITOYLATION.
    Tissue: Plasma.
  3. "The covalent structure of apolipoprotein A-I from canine high density lipoproteins."
    Chung H., Randolph A., Reardon I., Heinrikson R.L.
    J. Biol. Chem. 257:2961-2967(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-266.
  4. "The amino- and carboxyl-terminal sequences of canine apolipoprotein A-i."
    Nakai T., Whayne T.F., Tang J.
    FEBS Lett. 64:409-411(1976) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-57 AND 262-265.
  5. "HSC-2DPAGE and the two-dimensional gel electrophoresis database of dog heart proteins."
    Dunn M.J., Corbett J.M., Wheeler C.H.
    Electrophoresis 18:2795-2802(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-37.
    Tissue: Heart.

Entry informationi

Entry nameiAPOA1_CANFA
AccessioniPrimary (citable) accession number: P02648
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: December 15, 1998
Last modified: October 1, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3