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Protein

Troponin I, cardiac muscle

Gene

TNNI3

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei80Involved in TNI-TNT interactions1
Sitei97Involved in TNI-TNT interactions1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi137 – 149By similarityAdd BLAST13

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Muscle protein

Keywords - Ligandi

Actin-binding, Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Troponin I, cardiac muscle
Alternative name(s):
Cardiac troponin I
Gene namesi
Name:TNNI3
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001861541 – 211Troponin I, cardiac muscleAdd BLAST211

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylalanine1 Publication1
Modified residuei4PhosphoserineBy similarity1
Modified residuei22Phosphoserine; by PHK, PKA and PKD/PRKD12 Publications1
Modified residuei23Phosphoserine; by PKA and PKD/PRKD11 Publication1
Modified residuei26PhosphotyrosineBy similarity1
Modified residuei31Phosphothreonine; by STK4/MST1By similarity1
Modified residuei42Phosphoserine; by PKC/PRKCEBy similarity1
Modified residuei44Phosphoserine; by PKC/PRKCEBy similarity1
Modified residuei51Phosphothreonine; by STK4/MST1By similarity1
Modified residuei77PhosphoserineBy similarity1
Modified residuei78PhosphothreonineBy similarity1
Modified residuei129Phosphothreonine; by STK4/MST1By similarity1
Modified residuei143Phosphothreonine; by STK4/MST1By similarity1
Modified residuei151Phosphoserine; by PAK3By similarity1
Modified residuei182PhosphothreonineBy similarity1
Modified residuei200PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated at Ser-22 and Ser-23 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). Phosphorylated at Ser-42 and Ser-44 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity). Ser-22 is one of three sites in the region of residues 1-48 that are phosphorylated by phosphorylase kinase.By similarity2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP02646.

PTM databases

iPTMnetiP02646.

Interactioni

Subunit structurei

Interacts with TRIM63 (By similarity). Binds to actin and tropomyosin. Interacts with STK4/MST1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PASKQ96RG22EBI-8614386,EBI-1042651From a different organism.

Protein-protein interaction databases

IntActiP02646. 1 interactor.
MINTiMINT-8146737.

Structurei

3D structure databases

ProteinModelPortaliP02646.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni32 – 79Involved in binding TNCAdd BLAST48
Regioni129 – 150Involved in binding TNC and actinAdd BLAST22

Sequence similaritiesi

Belongs to the troponin I family.Curated

Phylogenomic databases

HOVERGENiHBG052737.
InParanoidiP02646.

Family and domain databases

InterProiIPR001978. Troponin.
IPR021666. Troponin-I_N.
[Graphical view]
PfamiPF00992. Troponin. 1 hit.
PF11636. Troponin-I_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P02646-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
ADESRDAAGE ARPAPAPVRR RSSANYRAYA TEPHAKSKKK ISASRKLQLK
60 70 80 90 100
TLMLQIAKQE LEREAEERRG EKGRALSTRC QPLELAGLGF AELQDLCRQL
110 120 130 140 150
HARVDKVDEE RYDVEAKVTK NITEIADLTQ KIFDLRGKFK RPTLRLRVRI
160 170 180 190 200
SADAMMQALL GTRAKETLDL RAHLKQVKKE DTEKENREVG DWRKNIDLLS
210
GMEGRKKKFE G
Length:211
Mass (Da):24,123
Last modified:September 13, 2004 - v2
Checksum:iA15B2683C53B2F1C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17Missing AA sequence (PubMed:1008822).Curated1
Sequence conflicti21 – 26RSSANY → SD AA sequence (PubMed:1008822).Curated6

Sequence databases

PIRiA90296. TPRBIC.

Cross-referencesi

Sequence databases

PIRiA90296. TPRBIC.

3D structure databases

ProteinModelPortaliP02646.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP02646. 1 interactor.
MINTiMINT-8146737.

PTM databases

iPTMnetiP02646.

Proteomic databases

PRIDEiP02646.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG052737.
InParanoidiP02646.

Family and domain databases

InterProiIPR001978. Troponin.
IPR021666. Troponin-I_N.
[Graphical view]
PfamiPF00992. Troponin. 1 hit.
PF11636. Troponin-I_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTNNI3_RABIT
AccessioniPrimary (citable) accession number: P02646
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: September 13, 2004
Last modified: October 5, 2016
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.