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Protein

Troponin T, fast skeletal muscle

Gene

TNNT3

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Muscle protein

Names & Taxonomyi

Protein namesi
Recommended name:
Troponin T, fast skeletal muscle
Short name:
TnTf
Alternative name(s):
Fast skeletal muscle troponin T
Short name:
fTnT
Gene namesi
Name:TNNT3
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved2 Publications
Chaini2 – 279278Troponin T, fast skeletal musclePRO_0000186180Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei2 – 21PhosphoserineBy similarity
Modified residuei98 – 981PhosphoserineBy similarity
Modified residuei169 – 1691PhosphoserineBy similarity
Modified residuei176 – 1761PhosphoserineBy similarity
Modified residuei177 – 1771PhosphoserineBy similarity
Modified residuei213 – 2131PhosphoserineBy similarity
Modified residuei229 – 2291PhosphotyrosineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

PTM databases

iPTMnetiP02641.

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000022512.

Structurei

3D structure databases

ProteinModelPortaliP02641.
SMRiP02641. Positions 80-113, 174-263.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the troponin T family.Curated

Phylogenomic databases

eggNOGiKOG3634. Eukaryota.
ENOG410XS6A. LUCA.
HOGENOMiHOG000231049.
HOVERGENiHBG052790.
InParanoidiP02641.

Family and domain databases

InterProiIPR027707. TNNT.
IPR027708. Tnnt3.
IPR001978. Troponin.
[Graphical view]
PANTHERiPTHR11521. PTHR11521. 1 hit.
PTHR11521:SF4. PTHR11521:SF4. 1 hit.
PfamiPF00992. Troponin. 1 hit.
[Graphical view]

Sequences (9)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 9 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform TnT1 (identifier: P02641-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDEEVEHVE EQYEEEEEAQ EEAPSPAEVH EPAPEVHVPE EVHEDALEDM
60 70 80 90 100
REEEEEEEKP RPKLTAPKIP EGEKVDFDDI QKKRQNKDLM ELQALIDSHF
110 120 130 140 150
EARKKEEEEL VALKERIEKR RAERAEQQRI RAEKERERQN RLAEEKARRE
160 170 180 190 200
EEDAKRRAEE DLKKKKALSS MGANYSSYLA KADQKRGKKQ TAREMKKKIL
210 220 230 240 250
AERRKPLNID HLSDEKLRDK AKELWDTLYQ LETDKFEFGE KLKRQKYDIM
260 270
NVRARVEMLA KFSKKAGTTA KGKVGGRWK
Length:279
Mass (Da):33,034
Last modified:January 23, 2007 - v4
Checksum:i1CF35712F2EE5281
GO
Isoform TnT1F (identifier: P02641-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     45-56: Missing.

Show »
Length:267
Mass (Da):31,558
Checksum:i93062FDF9B9E9788
GO
Isoform TnT2 (identifier: P02641-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     33-39: Missing.

Show »
Length:272
Mass (Da):32,304
Checksum:i58DC0B4D3B056450
GO
Isoform TnT2F (identifier: P02641-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     12-17: Missing.
     45-56: Missing.

Show »
Length:261
Mass (Da):30,750
Checksum:i66C6C865952E546F
GO
Isoform TnT2FA (identifier: P02641-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     33-39: Missing.
     45-56: Missing.

Show »
Length:260
Mass (Da):30,828
Checksum:i84BCA5FA9FA8AA66
GO
Isoform TnT2.5F (identifier: P02641-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     12-17: Missing.
     33-39: Missing.
     45-56: Missing.

Show »
Length:254
Mass (Da):30,020
Checksum:i730C371C8E65DB31
GO
Isoform TnT3 (identifier: P02641-7) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     23-39: Missing.

Show »
Length:262
Mass (Da):31,289
Checksum:iFD052B1246631107
GO
Isoform TnT3F (identifier: P02641-8) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     23-39: Missing.
     45-56: Missing.

Show »
Length:250
Mass (Da):29,813
Checksum:iBB0EBF385076ADCB
GO
Isoform TnT4F (identifier: P02641-9) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     12-17: Missing.
     23-39: Missing.
     45-56: Missing.

Show »
Length:244
Mass (Da):29,005
Checksum:i934314CF18FD0556
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti20 – 201Q → E AA sequence (PubMed:320204).Curated
Sequence conflicti36 – 372VH → HV AA sequence (PubMed:320204).Curated
Sequence conflicti36 – 372VH → HV AA sequence (PubMed:2704041).Curated
Sequence conflicti62 – 621Missing AA sequence (PubMed:320204).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei12 – 176Missing in isoform TnT2F, isoform TnT2.5F and isoform TnT4F. CuratedVSP_006653
Alternative sequencei23 – 3917Missing in isoform TnT3, isoform TnT3F and isoform TnT4F. CuratedVSP_006654Add
BLAST
Alternative sequencei33 – 397Missing in isoform TnT2, isoform TnT2FA and isoform TnT2.5F. CuratedVSP_006655
Alternative sequencei45 – 5612Missing in isoform TnT1F, isoform TnT2F, isoform TnT2FA, isoform TnT2.5F, isoform TnT3F and isoform TnT4F. CuratedVSP_006656Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04970 mRNA. Translation: AAA16023.1.
U04971 mRNA. Translation: AAA16024.1.
U04972 mRNA. Translation: AAA16025.1.
U04973 mRNA. Translation: AAA16026.1.
U04974 mRNA. Translation: AAA16027.1.
U04975 mRNA. Translation: AAA16028.1.
U04976 mRNA. Translation: AAA16029.1.
U04977 mRNA. Translation: AAA16030.1.
U04978 mRNA. Translation: AAA16031.1.
UniGeneiOcu.1806.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04970 mRNA. Translation: AAA16023.1.
U04971 mRNA. Translation: AAA16024.1.
U04972 mRNA. Translation: AAA16025.1.
U04973 mRNA. Translation: AAA16026.1.
U04974 mRNA. Translation: AAA16027.1.
U04975 mRNA. Translation: AAA16028.1.
U04976 mRNA. Translation: AAA16029.1.
U04977 mRNA. Translation: AAA16030.1.
U04978 mRNA. Translation: AAA16031.1.
UniGeneiOcu.1806.

3D structure databases

ProteinModelPortaliP02641.
SMRiP02641. Positions 80-113, 174-263.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000022512.

PTM databases

iPTMnetiP02641.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG3634. Eukaryota.
ENOG410XS6A. LUCA.
HOGENOMiHOG000231049.
HOVERGENiHBG052790.
InParanoidiP02641.

Family and domain databases

InterProiIPR027707. TNNT.
IPR027708. Tnnt3.
IPR001978. Troponin.
[Graphical view]
PANTHERiPTHR11521. PTHR11521. 1 hit.
PTHR11521:SF4. PTHR11521:SF4. 1 hit.
PfamiPF00992. Troponin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Primary structure of rabbit skeletal muscle troponin-T. Sequence determination of the NH2-terminal fragment CB3 and the complete sequence of troponin-T."
    Pearlstone J.R., Johnson P., Carpenter M.R., Smillie L.B.
    J. Biol. Chem. 252:983-989(1977) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-11; 18-44 AND 57-279.
  2. "Origin of fetal troponin T: developmentally regulated splicing of a new exon in the fast troponin T gene."
    Briggs M.M., Schachat F.
    Dev. Biol. 158:503-509(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-67, ALTERNATIVE SPLICING.
    Strain: New Zealand white.
    Tissue: Skeletal muscle.
  3. "Transitions from fetal to fast troponin T isoforms are coordinated with changes in tropomyosin and alpha-actinin isoforms in developing rabbit skeletal muscle."
    Briggs M.M., McGinnis H.D., Schachat F.
    Dev. Biol. 140:253-260(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-67, ALTERNATIVE SPLICING.
    Strain: New Zealand white.
    Tissue: Skeletal muscle.
  4. Briggs M.M., Schachat F.
    Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-67, ALTERNATIVE SPLICING.
    Strain: New Zealand white.
    Tissue: Skeletal muscle.
  5. "N-terminal amino acid sequences of three functionally different troponin T isoforms from rabbit fast skeletal muscle."
    Briggs M.M., Schachat F.
    J. Mol. Biol. 206:245-249(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-44 AND 57-66, ACETYLATION AT SER-2, CLEAVAGE OF INITIATOR METHIONINE.
    Strain: New Zealand white.
    Tissue: Skeletal muscle.

Entry informationi

Entry nameiTNNT3_RABIT
AccessioniPrimary (citable) accession number: P02641
Secondary accession number(s): P19349, P19350
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: January 20, 2016
This is version 96 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.