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Protein

Protein S100-A1

Gene

S100A1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi20 – 33141; low affinityAdd
BLAST
Calcium bindingi63 – 74122; high affinityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein S100-A1
Alternative name(s):
S-100 protein alpha chain
S-100 protein subunit alpha
S100 calcium-binding protein A1
Gene namesi
Name:S100A1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 9493Protein S100-A1PRO_0000143960Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21Blocked amino end (Gly)1 Publication
Modified residuei86 – 861S-nitrosocysteineBy similarity

Keywords - PTMi

S-nitrosylation

Proteomic databases

PaxDbiP02639.
PRIDEiP02639.

Expressioni

Tissue specificityi

Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.

Gene expression databases

ExpressionAtlasiP02639. baseline and differential.

Interactioni

Subunit structurei

Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. Also forms heterodimers with S100P. Interacts with AGER and CAPZA1. Interacts with FKBP4. Interacts with RYR1 and RYR2. Interacts with CACYBP in a calcium-dependent manner. Interacts with PPP5C (via TPR repeats); the interaction is calcium-dependent and modulates PPP5C activity (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CacybpQ9CXW34EBI-6477285,EBI-767146From a different organism.
FKBP4Q9TRY02EBI-6477285,EBI-6477371
HSP90AA1Q76LV22EBI-6477285,EBI-6477314
HSPA1AQ279752EBI-6477285,EBI-6477341
PPIDP268822EBI-6477285,EBI-6477155
RYR1P117163EBI-6477285,EBI-6477441From a different organism.

Protein-protein interaction databases

IntActiP02639. 6 interactions.
STRINGi9913.ENSBTAP00000044226.

Structurei

Secondary structure

1
94
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1815Combined sources
Helixi31 – 4212Combined sources
Turni43 – 453Combined sources
Helixi46 – 494Combined sources
Helixi54 – 6411Combined sources
Helixi75 – 9016Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JPTNMR-A/B2-94[»]
ProteinModelPortaliP02639.
SMRiP02639. Positions 2-94.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02639.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 4836EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini50 – 8536EF-hand 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the S-100 family.Curated
Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IXZ2. Eukaryota.
ENOG4111987. LUCA.
GeneTreeiENSGT00760000119034.
HOGENOMiHOG000246968.
HOVERGENiHBG001479.
InParanoidiP02639.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028486. S100-A1.
IPR001751. S100/CaBP-9k_CS.
IPR013787. S100_Ca-bd_sub.
[Graphical view]
PANTHERiPTHR11639:SF66. PTHR11639:SF66. 1 hit.
PfamiPF00036. EF-hand_1. 1 hit.
PF01023. S_100. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 1 hit.
SM01394. S_100. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02639-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSELETAME TLINVFHAHS GKEGDKYKLS KKELKELLQT ELSGFLDAQK
60 70 80 90
DADAVDKVMK ELDENGDGEV DFQEYVVLVA ALTVACNNFF WENS
Length:94
Mass (Da):10,518
Last modified:January 23, 2007 - v2
Checksum:iAD5E400DB85025D2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651N → D AA sequence (PubMed:7250124).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC141991 mRNA. Translation: AAI41992.1.
BC148019 mRNA. Translation: AAI48020.1.
PIRiA24156. BCBOIA.
RefSeqiNP_001092512.1. NM_001099042.2.
XP_005203778.1. XM_005203721.3.
XP_010801217.1. XM_010802915.2.
UniGeneiBt.35032.

Genome annotation databases

EnsembliENSBTAT00000006806; ENSBTAP00000006806; ENSBTAG00000005163.
GeneIDi528735.
KEGGibta:104968698.
bta:528735.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC141991 mRNA. Translation: AAI41992.1.
BC148019 mRNA. Translation: AAI48020.1.
PIRiA24156. BCBOIA.
RefSeqiNP_001092512.1. NM_001099042.2.
XP_005203778.1. XM_005203721.3.
XP_010801217.1. XM_010802915.2.
UniGeneiBt.35032.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JPTNMR-A/B2-94[»]
ProteinModelPortaliP02639.
SMRiP02639. Positions 2-94.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP02639. 6 interactions.
STRINGi9913.ENSBTAP00000044226.

Proteomic databases

PaxDbiP02639.
PRIDEiP02639.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000006806; ENSBTAP00000006806; ENSBTAG00000005163.
GeneIDi528735.
KEGGibta:104968698.
bta:528735.

Organism-specific databases

CTDi6271.

Phylogenomic databases

eggNOGiENOG410IXZ2. Eukaryota.
ENOG4111987. LUCA.
GeneTreeiENSGT00760000119034.
HOGENOMiHOG000246968.
HOVERGENiHBG001479.
InParanoidiP02639.

Miscellaneous databases

EvolutionaryTraceiP02639.
NextBioi20874888.

Gene expression databases

ExpressionAtlasiP02639. baseline and differential.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028486. S100-A1.
IPR001751. S100/CaBP-9k_CS.
IPR013787. S100_Ca-bd_sub.
[Graphical view]
PANTHERiPTHR11639:SF66. PTHR11639:SF66. 1 hit.
PfamiPF00036. EF-hand_1. 1 hit.
PF01023. S_100. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 1 hit.
SM01394. S_100. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal cerebellum and Hypothalamus.
  3. "The amino-acid sequence of the alpha subunit in bovine brain S-100a protein."
    Isobe T., Okuyama T.
    Eur. J. Biochem. 116:79-86(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-94.
  4. "Ions binding to S100 proteins: structural changes induced by calcium and zinc on S100a and S100b proteins."
    Baudier J., Gerard D.
    Biochemistry 22:3360-3369(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: METAL ION-BINDING PROPERTIES.
  5. "Structural and motional changes induced in apo-S100A1 protein by the disulfide formation between its Cys 85 residue and beta-mercaptoethanol."
    Zhukov I., Ejchart A., Bierzynski A.
    Biochemistry 47:640-650(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 2-94.

Entry informationi

Entry nameiS10A1_BOVIN
AccessioniPrimary (citable) accession number: P02639
Secondary accession number(s): A5PJ73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.