P02634 (S100G_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein S100-G

Alternative name(s):
9 kDa CaBP
Calbindin-D9k
Cholecalcin
S100 calcium-binding protein G
Vitamin D-dependent calcium-binding protein, intestinal
Short name=CABP

Gene names

Name:	S100g
Synonyms:	Calb3, S100d

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	79 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Miscellaneous	The synthesis of this protein in the absorptive cells of the rat duodenum is vitamin D3-dependent.
Sequence similarities	Belongs to the S-101 family. Contains 2 EF-hand domains.
Caution	Was originally thought to originate from chick (Ref.7).

Ontologies

Keywords
Domain	Repeat
Ligand	Calcium Metal-binding Vitamin D
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	apical plasma membrane Inferred from electronic annotation. Source: Compara basolateral plasma membrane Inferred from electronic annotation. Source: Compara
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro vitamin D binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5
	Chain	2 – 79	78	Protein S100-G		PRO_0000144030
Regions
	Domain	13 – 48	36	EF-hand 1
	Domain	45 – 79	35	EF-hand 2
	Calcium binding	18 – 31	14	1; low affinity
	Calcium binding	58 – 69	12	2; high affinity
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.5
Experimental info
	Sequence conflict	2 – 3	2	SA → AS AA sequence Ref.5
	Sequence conflict	42	1	S → N in AAA40843. Ref.6
	Sequence conflict	45	1	K → KQ in AAA40853. Ref.3
	Sequence conflict	55	1	K → E in AAA40843. Ref.6
	Sequence conflict	60	1	N → D AA sequence Ref.5
	Sequence conflict	61	1	G → D in AAA40843. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P02634 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 3566BD81668704C6	FASTA	79	9,038
	10 20 30 40 50 60 MSAKKSPEEM KSIFQKYAAK EGDPNQLSKE ELKLLIQSEF PSLLKASSTL DNLFKELDKN 70 GDGEVSYEEF EVFFKKLSQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The rat vitamin-D-dependent calcium-binding protein (9-kDa CaBP) gene. Complete nucleotide sequence and structural organization." Perret C., Lomri N., Gouhier N., Auffray C., Thomasset M. Eur. J. Biochem. 172:43-51(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular cloning of the cDNA and chromosomal gene for vitamin D-dependent calcium-binding protein of rat intestine." Darwish H.M., Krisinger J., Strom M., Deluca H.F. Proc. Natl. Acad. Sci. U.S.A. 84:6108-6111(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Structure and nucleotide sequence of the rat intestinal vitamin D-dependent calcium binding protein gene." Krisinger J., Darwish H., Maeda N., Deluca H.F. Proc. Natl. Acad. Sci. U.S.A. 85:8988-8992(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
[5]	"The purification and complete amino acid sequence of the 9000-Mr Ca2+-binding protein from rat placenta. Identity with the vitamin D-dependent intestinal Ca2+-binding protein." McManus J.P., Watson D.C., Yaguchi M. Biochem. J. 235:585-595(1986) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-79, ACETYLATION AT SER-2.
[6]	"Sequence of rat intestinal vitamin D-dependent calcium-binding protein derived from a cDNA clone. Evolutionary implications." Desplan C., Heidmann O., Lillie J.W., Auffray C., Thomasset M. J. Biol. Chem. 258:13502-13505(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-79.
[7]	"cDNA sequence identity of a vitamin D-dependent calcium-binding protein in the chick to calbindin D-9K." Zanello S.B., Boland R.L., Norman A.W. Endocrinology 136:2784-2787(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 22-71.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X16635 Genomic DNA. Translation: CAA34627.1. K00994 mRNA. Translation: AAA40843.1. J02954 mRNA. Translation: AAA42333.1. BC059153 mRNA. Translation: AAH59153.1. J04133 Genomic DNA. Translation: AAA40853.1. S78183 mRNA. Translation: AAD14276.1.
IPI	IPI00231660.
PIR	KLRTI. A31258.
RefSeq	NP_036653.1. NM_012521.2.
UniGene	Rn.9974.
3D structure databases
ProteinModelPortal	P02634.
SMR	P02634. Positions 2-79.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000005622.
Proteomic databases
PRIDE	P02634.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000005622; ENSRNOP00000005622; ENSRNOG00000004222.
GeneID	24249.
KEGG	rno:24249.
UCSC	RGD:2253. rat.
Organism-specific databases
CTD	795.
RGD	2253. S100g.
Phylogenomic databases
eggNOG	NOG39449.
GeneTree	ENSGT00530000064238.
HOGENOM	HOG000246968.
HOVERGEN	HBG001479.
InParanoid	P02634.
KO	K14734.
OMA	KGPSTLD.
OrthoDB	EOG4NP754.
Gene expression databases
Genevestigator	P02634.
GermOnline	ENSRNOG00000004222. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR001751. S100/CaBP-9k_CS. IPR013787. S100_Ca-bd_sub. [Graphical view]
Pfam	PF00036. efhand. 1 hit. PF01023. S_100. 1 hit. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 1 hit. PS00303. S100_CABP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	602757.

Entry information

Entry name

S100G_RAT

Accession

Primary (citable) accession number: P02634
Secondary accession number(s): P51964

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 106 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program