P02620 (PRVB_MERME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Parvalbumin beta
Organism	Merluccius merluccius (European hake)
Taxonomic identifier	8063 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Euteleostei › Neognathi › Neoteleostei › Eurypterygii › Ctenosquamata › Acanthomorpha › Euacanthomorpha › Holacanthopterygii › Paracanthopterygii › Gadiformes › Merlucciidae › Merlucciinae › Merluccius

Protein attributes

Sequence length	108 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.
Miscellaneous	This is the major hake parvalbumin. This parvalbumin has an isoelectric point of 4.36. Is regarded as an important allergen.
Sequence similarities	Belongs to the parvalbumin family. Contains 2 EF-hand domains.
Mass spectrometry	Molecular mass is 11329.746±0.0013 Da from positions 1 - 108. Determined by ESI. Ref.2

Ontologies

Keywords
Disease	Allergen
Domain	Repeat
Ligand	Calcium Metal-binding
Molecular function	Muscle protein
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 108	108	Parvalbumin beta		PRO_0000073614
Regions
	Domain	38 – 73	36	EF-hand 1
	Domain	77 – 108	32	EF-hand 2
	Calcium binding	51 – 62	12	1 By similarity
	Calcium binding	90 – 101	12	2 By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylalanine Ref.1 Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P02620 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: E5526ACBF3DC3B64	FASTA	108	11,295
	10 20 30 40 50 60 AFAGILADAD ITAALAACKA EGSFKHGEFF TKIGLKGKSA ADIKKVFGII DQDKSDFVEE 70 80 90 100 DELKLFLQNF SAGARALTDA ETATFLKAGD SDGDGKIGVE EFAAMVKG

References

[1]	"The primary structure of the major parvalbumin from hake muscle. Overlapping peptides obtained with chemical and enzymatic methods. The complete amino-acid sequence." Capony J.-P., Ryden L., Demaille J.G., Pechere J.-F. Eur. J. Biochem. 32:97-108(1973) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Extensive de novo sequencing of new parvalbumin isoforms using a novel combination of bottom-up proteomics, accurate molecular mass measurement by FTICR-MS, and selected MS/MS ion monitoring." Carrera M., Canas B., Vazquez J., Gallardo J.M. J. Proteome Res. 9:4393-4406(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, MASS SPECTROMETRY, ACETYLATION AT ALA-1. Tissue: Muscle.

Cross-references

Sequence databases
PIR	PVHK. A03055.
3D structure databases
ProteinModelPortal	P02620.
SMR	P02620. Positions 1-108.
ModBase	Search...
Protein family/group databases
Allergome	7643. Mer mr 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG107490.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR008080. Parvalbumin. [Graphical view]
PANTHER	PTHR11653. PTHR11653. 1 hit.
Pfam	PF13499. EF_hand_5. 1 hit. [Graphical view]
SMART	SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRVB_MERME

Accession

Primary (citable) accession number: P02620

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program