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Protein

Myosin regulatory light chain 2, smooth muscle major isoform

Gene
N/A
Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity. Implicated in cytokinesis, receptor capping, and cell locomotion (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi42 – 5312Add
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-GGA-445355. Smooth Muscle Contraction.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin regulatory light chain 2, smooth muscle major isoform
Short name:
MLC-2
Alternative name(s):
DTNB
G1
Isoform L20-A
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 20

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 172171Myosin regulatory light chain 2, smooth muscle major isoformPRO_0000198731Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP02612.
PRIDEiP02612.

PTM databases

iPTMnetiP02612.

Interactioni

Subunit structurei

Myosin is a hexamer of 2 heavy chains and 4 light chains.

Protein-protein interaction databases

IntActiP02612. 1 interaction.
STRINGi9031.ENSGALP00000034131.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J04electron microscopy-B/E26-168[»]
ProteinModelPortaliP02612.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 6436EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini98 – 13336EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini134 – 16936EF-hand 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0031. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000119196.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiP02612.
KOiK12755.
OMAiRINADNE.
PhylomeDBiP02612.
TreeFamiTF314218.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02612-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSKRAKAKT TKKRPQRATS NVFAMFDQSQ IQEFKEAFNM IDQNRDGFID
60 70 80 90 100
KEDLHDMLAS MGKNPTDEYL EGMMSEAPGP INFTMFLTMF GEKLNGTDPE
110 120 130 140 150
DVIRNAFACF DEEASGFIHE DHLRELLTTM GDRFTDEEVD EMYREAPIDK
160 170
KGNFNYVEFT RILKHGAKDK DD
Length:172
Mass (Da):19,845
Last modified:January 23, 2007 - v2
Checksum:i3B858F333CE3D1D9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 1712AKAKT…KRPQR → KRPQRAKAKTTK AA sequence (PubMed:7274219).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06387 mRNA. Translation: CAA29684.1.
Y00983 mRNA. Translation: CAB46269.1.
PIRiS03184. MOCHG1.
RefSeqiNP_990609.1. NM_205278.1.
XP_015151726.1. XM_015296240.1.
XP_015151727.1. XM_015296241.1.
UniGeneiGga.4052.

Genome annotation databases

EnsembliENSGALT00000043998; ENSGALP00000041913; ENSGALG00000028567.
GeneIDi396215.
KEGGigga:396215.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06387 mRNA. Translation: CAA29684.1.
Y00983 mRNA. Translation: CAB46269.1.
PIRiS03184. MOCHG1.
RefSeqiNP_990609.1. NM_205278.1.
XP_015151726.1. XM_015296240.1.
XP_015151727.1. XM_015296241.1.
UniGeneiGga.4052.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J04electron microscopy-B/E26-168[»]
ProteinModelPortaliP02612.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP02612. 1 interaction.
STRINGi9031.ENSGALP00000034131.

PTM databases

iPTMnetiP02612.

Proteomic databases

PaxDbiP02612.
PRIDEiP02612.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000043998; ENSGALP00000041913; ENSGALG00000028567.
GeneIDi396215.
KEGGigga:396215.

Organism-specific databases

CTDi10398.

Phylogenomic databases

eggNOGiKOG0031. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000119196.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiP02612.
KOiK12755.
OMAiRINADNE.
PhylomeDBiP02612.
TreeFamiTF314218.

Enzyme and pathway databases

ReactomeiR-GGA-445355. Smooth Muscle Contraction.

Miscellaneous databases

PROiP02612.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The nucleotide sequence of chicken smooth muscle myosin light chain two."
    Zavodny P.J., Petro M.E., Kumar C.C., Dailey S.H., Lonial H.K., Narula S.K., Leibowitz P.J.
    Nucleic Acids Res. 16:1214-1214(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Molecular cloning and sequencing of the chicken smooth muscle myosin regulatory light chain."
    Messer N.G., Kendrick-Jones J.
    FEBS Lett. 234:49-52(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Amino-acid sequence of the 20 000-molecular-weight light chain of chicken gizzard-muscle myosin."
    Maita T., Chen J., Matsuda G.
    Eur. J. Biochem. 117:417-424(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-172, ACETYLATION AT SER-2.
  4. "Phosphorylation site sequence of smooth muscle myosin light chain (Mr = 20 000)."
    Pearson R.B., Jakes R., John M., Kendrick-Jones J., Kemp B.
    FEBS Lett. 168:108-112(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  5. "Two isoforms of smooth muscle myosin regulatory light chain in chicken gizzard."
    Inoue A., Yanagisawa M., Takano-Ohmuro H., Masaki T.
    Eur. J. Biochem. 183:645-651(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 64-73.
    Tissue: Embryonic gizzard.

Entry informationi

Entry nameiMLRM_CHICK
AccessioniPrimary (citable) accession number: P02612
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This chain binds calcium.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.