Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P02609

- MLRS_CHICK

UniProt

P02609 - MLRS_CHICK

Protein

Myosin regulatory light chain 2, skeletal muscle isoform

Gene

MYLPF

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (19 Jul 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi37 – 4812Add
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro

    Keywords - Molecular functioni

    Motor protein, Muscle protein, Myosin

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myosin regulatory light chain 2, skeletal muscle isoform
    Alternative name(s):
    DTNB
    Fast skeletal myosin light chain 2
    Short name:
    MLC-2
    G2
    LC2f
    Gene namesi
    Name:MYLPF
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. myosin complex Source: UniProtKB-KW

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 168167Myosin regulatory light chain 2, skeletal muscle isoformPRO_0000019308Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N,N,N-trimethylalanine1 Publication
    Modified residuei15 – 151PhosphoserineSequence Analysis

    Post-translational modificationi

    The N-terminus is blocked. N,N,N-trimethylalanine, found in other myosin light chains would not have been detected in the N-terminal tryptic peptide in PubMed:7358336 because it would remain trimethylated and ninhydrin negative after hydrolysis.

    Keywords - PTMi

    Methylation, Phosphoprotein

    Proteomic databases

    PRIDEiP02609.

    Interactioni

    Subunit structurei

    Myosin is a hexamer of 2 heavy chains and 4 light chains.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ALMmodel-B3-168[»]
    1I84electron microscopy20.00U/Z3-168[»]
    1M8Qelectron microscopy70.00B/E/H/Q21-164[»]
    1MVWelectron microscopy70.00B/E/H/K/N/Q26-164[»]
    1O18electron microscopy70.00E/H/K/N/Q26-164[»]
    1O19electron microscopy70.00B/E/H/K/N/T26-164[»]
    1O1Aelectron microscopy70.00B/E/H/K/N/Q26-164[»]
    1O1Belectron microscopy70.00B/E/H/K26-164[»]
    1O1Celectron microscopy70.00B/E/H/K/Q26-164[»]
    1O1Delectron microscopy70.00B/E/H/K/N/Q26-164[»]
    1O1Eelectron microscopy70.00B/E/H/K/N/Q26-164[»]
    1O1Felectron microscopy70.00B/E/H/K26-164[»]
    1O1Gelectron microscopy70.00B/E/H/K/N/Q26-164[»]
    2MYSX-ray2.80B3-168[»]
    2W4Aelectron microscopy35.00B16-165[»]
    2W4Gelectron microscopy35.00B16-165[»]
    2W4Helectron microscopy35.00B16-165[»]
    ProteinModelPortaliP02609.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP02609.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini24 – 5936EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini94 – 12936EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini130 – 16536EF-hand 3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 3 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    HOVERGENiHBG012180.
    KOiK12758.
    PhylomeDBiP02609.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view]
    PfamiPF13405. EF-hand_6. 1 hit.
    [Graphical view]
    SMARTiSM00054. EFh. 2 hits.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P02609-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPKKAKRRA AEGSSNVFSM FDQTQIQEFK EAFTVIDQNR DGIIDKDDLR    50
    ETFAAMGRLN VKNEELDAMI KEASGPINFT VFLTMFGEKL KGADPEDVIM 100
    GAFKVLDPDG KGSIKKSFLE ELLTTQCDRF TPEEIKNMWA AFPPDVAGNV 150
    DYKNICYVIT HGEDKEGE 168
    Length:168
    Mass (Da):18,839
    Last modified:July 19, 2004 - v2
    Checksum:iFF5913861D2795F1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti61 – 611V → L in AAA48980. (PubMed:2580100)Curated
    Sequence conflicti103 – 1031F → L in AAA48980. (PubMed:2580100)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11030 mRNA. Translation: AAA48980.1.
    PIRiI50393. MOCHLS.
    RefSeqiNP_001185673.1. NM_001198744.1.
    UniGeneiGga.839.

    Genome annotation databases

    GeneIDi776775.
    KEGGigga:776775.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11030 mRNA. Translation: AAA48980.1 .
    PIRi I50393. MOCHLS.
    RefSeqi NP_001185673.1. NM_001198744.1.
    UniGenei Gga.839.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ALM model - B 3-168 [» ]
    1I84 electron microscopy 20.00 U/Z 3-168 [» ]
    1M8Q electron microscopy 70.00 B/E/H/Q 21-164 [» ]
    1MVW electron microscopy 70.00 B/E/H/K/N/Q 26-164 [» ]
    1O18 electron microscopy 70.00 E/H/K/N/Q 26-164 [» ]
    1O19 electron microscopy 70.00 B/E/H/K/N/T 26-164 [» ]
    1O1A electron microscopy 70.00 B/E/H/K/N/Q 26-164 [» ]
    1O1B electron microscopy 70.00 B/E/H/K 26-164 [» ]
    1O1C electron microscopy 70.00 B/E/H/K/Q 26-164 [» ]
    1O1D electron microscopy 70.00 B/E/H/K/N/Q 26-164 [» ]
    1O1E electron microscopy 70.00 B/E/H/K/N/Q 26-164 [» ]
    1O1F electron microscopy 70.00 B/E/H/K 26-164 [» ]
    1O1G electron microscopy 70.00 B/E/H/K/N/Q 26-164 [» ]
    2MYS X-ray 2.80 B 3-168 [» ]
    2W4A electron microscopy 35.00 B 16-165 [» ]
    2W4G electron microscopy 35.00 B 16-165 [» ]
    2W4H electron microscopy 35.00 B 16-165 [» ]
    ProteinModelPortali P02609.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P02609.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 776775.
    KEGGi gga:776775.

    Organism-specific databases

    CTDi 29895.

    Phylogenomic databases

    HOVERGENi HBG012180.
    KOi K12758.
    PhylomeDBi P02609.

    Miscellaneous databases

    EvolutionaryTracei P02609.
    NextBioi 20923396.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view ]
    Pfami PF13405. EF-hand_6. 1 hit.
    [Graphical view ]
    SMARTi SM00054. EFh. 2 hits.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Recombinant DNA approach for defining the primary structure of monoclonal antibody epitopes."
      Reinach F.C., Fischman D.A.
      J. Mol. Biol. 181:411-422(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The amino acid sequence of the L-2 light chain of chicken skeletal muscle myosin."
      Suzuyama Y., Umegane T., Maita T., Matsuda G.
      Hoppe-Seyler's Z. Physiol. Chem. 361:119-127(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 3-168.
    3. "The widespread distribution of alpha-N-trimethylalanine as the N-terminal amino acid of light chains from vertebrate striated muscle myosins."
      Henry G.D., Trayer I.P., Brewer S., Levine B.A.
      Eur. J. Biochem. 148:75-82(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: METHYLATION AT ALA-2.
    4. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).

    Entry informationi

    Entry nameiMLRS_CHICK
    AccessioniPrimary (citable) accession number: P02609
    Secondary accession number(s): Q90915
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This chain binds calcium.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3