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P02605

- MLE3_CHICK

UniProt

P02605 - MLE3_CHICK

Protein

Myosin light chain 3, skeletal muscle isoform

Gene
N/A
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro

    Keywords - Molecular functioni

    Motor protein, Muscle protein, Myosin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myosin light chain 3, skeletal muscle isoform
    Alternative name(s):
    Alkali myosin light chain 3
    Short name:
    MLC-3
    Myosin light chain 3f
    Myosin light chain alkali 2
    Short name:
    Myosin light chain A2
    Skeletal-muscle myosin L-4 light chain
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. myosin complex Source: UniProtKB-KW

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 150149Myosin light chain 3, skeletal muscle isoformPRO_0000198705Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21Blocked amino end (Ser)

    Post-translational modificationi

    The N-terminus is blocked.

    Proteomic databases

    PRIDEiP02605.

    Interactioni

    Subunit structurei

    Myosin is a hexamer of 2 heavy chains and 4 light chains.

    Structurei

    Secondary structure

    1
    150
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi9 – 179
    Beta strandi21 – 233
    Helixi27 – 3610
    Helixi43 – 519
    Turni52 – 543
    Beta strandi57 – 593
    Helixi65 – 7511
    Helixi76 – 816
    Helixi84 – 9411
    Beta strandi97 – 993
    Beta strandi101 – 1033
    Helixi104 – 11310
    Beta strandi114 – 1163
    Helixi120 – 1278
    Beta strandi135 – 1384
    Helixi139 – 1468

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1MVWelectron microscopy70.00C/F/I/L/O/R10-150[»]
    1O18electron microscopy70.00F/I/L/O/R10-150[»]
    1O19electron microscopy70.00C/F/I/L/O/U10-150[»]
    1O1Aelectron microscopy70.00C/F/I/L/O/R10-150[»]
    1O1Belectron microscopy70.00C/F/I/L10-150[»]
    1O1Celectron microscopy70.00C/F/I/L/R10-150[»]
    1O1Delectron microscopy70.00C/F/I/L/O/R10-150[»]
    1O1Eelectron microscopy70.00C/F/I/L/O/R10-150[»]
    1O1Felectron microscopy70.00C/F/I/L10-150[»]
    1O1Gelectron microscopy70.00C/F/I/L/O/R10-150[»]
    2W4Aelectron microscopy35.00C6-150[»]
    2W4Gelectron microscopy35.00C6-150[»]
    2W4Helectron microscopy35.00C6-150[»]
    ProteinModelPortaliP02605.
    SMRiP02605. Positions 3-149.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP02605.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini6 – 4136EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini44 – 7936EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini83 – 11836EF-hand 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini118 – 15033EF-hand 4PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 4 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    HOGENOMiHOG000233018.
    HOVERGENiHBG012180.
    KOiK05738.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR002048. EF_hand_dom.
    [Graphical view]
    PfamiPF13405. EF-hand_6. 1 hit.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view]
    SMARTiSM00054. EFh. 3 hits.
    [Graphical view]
    PROSITEiPS50222. EF_HAND_2. 4 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform MLC3 (identifier: P02605-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSFSPDEIND FKEAFLLFDR TGDAKITLSQ VGDIVRALGQ NPTNAEINKI    50
    LGNPSKEEMN AKKITFEEFL PMLQAAANNK DQGTFEDFVE GLRVFDKEGN 100
    GTVMGAELRH VLATLGEKMT EEEVEELMKG QEDSNGCINY EAFVKHIMSV 150
    Length:150
    Mass (Da):16,710
    Last modified:January 23, 2007 - v3
    Checksum:i6F7BE6BA70055BE0
    GO
    Isoform MLC1 (identifier: P02604-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry P02604.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:192
    Mass (Da):20,899
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti7 – 93EIN → QID AA sequence (PubMed:6783403)Curated
    Sequence conflicti74 – 741Q → QM in AAA48956. (PubMed:6709041)Curated
    Sequence conflicti104 – 1041Missing in AAA48956. (PubMed:6709041)Curated
    Sequence conflicti128 – 1281M → I in AAA48961. (PubMed:6709041)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J00888 mRNA. Translation: AAA48961.1.
    K02610, K02609 Genomic DNA. Translation: AAA48956.1.
    PIRiI50384. MOCHA2.
    RefSeqiNP_001038097.1. NM_001044632.1.
    UniGeneiGga.4835.

    Genome annotation databases

    GeneIDi396470.
    KEGGigga:396470.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J00888 mRNA. Translation: AAA48961.1 .
    K02610 , K02609 Genomic DNA. Translation: AAA48956.1 .
    PIRi I50384. MOCHA2.
    RefSeqi NP_001038097.1. NM_001044632.1.
    UniGenei Gga.4835.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1MVW electron microscopy 70.00 C/F/I/L/O/R 10-150 [» ]
    1O18 electron microscopy 70.00 F/I/L/O/R 10-150 [» ]
    1O19 electron microscopy 70.00 C/F/I/L/O/U 10-150 [» ]
    1O1A electron microscopy 70.00 C/F/I/L/O/R 10-150 [» ]
    1O1B electron microscopy 70.00 C/F/I/L 10-150 [» ]
    1O1C electron microscopy 70.00 C/F/I/L/R 10-150 [» ]
    1O1D electron microscopy 70.00 C/F/I/L/O/R 10-150 [» ]
    1O1E electron microscopy 70.00 C/F/I/L/O/R 10-150 [» ]
    1O1F electron microscopy 70.00 C/F/I/L 10-150 [» ]
    1O1G electron microscopy 70.00 C/F/I/L/O/R 10-150 [» ]
    2W4A electron microscopy 35.00 C 6-150 [» ]
    2W4G electron microscopy 35.00 C 6-150 [» ]
    2W4H electron microscopy 35.00 C 6-150 [» ]
    ProteinModelPortali P02605.
    SMRi P02605. Positions 3-149.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P02605.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 396470.
    KEGGi gga:396470.

    Organism-specific databases

    CTDi 4632.

    Phylogenomic databases

    HOGENOMi HOG000233018.
    HOVERGENi HBG012180.
    KOi K05738.

    Miscellaneous databases

    EvolutionaryTracei P02605.
    NextBioi 20816509.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR002048. EF_hand_dom.
    [Graphical view ]
    Pfami PF13405. EF-hand_6. 1 hit.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view ]
    SMARTi SM00054. EFh. 3 hits.
    [Graphical view ]
    PROSITEi PS50222. EF_HAND_2. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and nucleotide sequences of the complementary DNAs to chicken skeletal muscle myosin two alkali light chain mRNAs."
      Nabeshima Y., Fujii-Kuriyama Y., Muramatsu M., Ogata K.
      Nucleic Acids Res. 10:6099-6110(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Alternative transcription and two modes of splicing results in two myosin light chains from one gene."
      Nabeshima Y., Fujii-Kuriyama Y., Muramatsu M., Ogata K.
      Nature 308:333-338(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Skeletal muscle.
    3. "Amino-acid sequence of the L-4 light chain of chicken skeletal-muscle myosin."
      Maita T., Umegane T., Matsuda G.
      Eur. J. Biochem. 114:45-49(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-150, BLOCKAGE OF N-TERMINUS.
      Tissue: Skeletal muscle.

    Entry informationi

    Entry nameiMLE3_CHICK
    AccessioniPrimary (citable) accession number: P02605
    Secondary accession number(s): Q90896
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 110 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3