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P02605 (MLE3_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myosin light chain 3, skeletal muscle isoform
Alternative name(s):
Alkali myosin light chain 3
Short name=MLC-3
Myosin light chain 3f
Myosin light chain alkali 2
Short name=Myosin light chain A2
Skeletal-muscle myosin L-4 light chain
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length150 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Myosin is a hexamer of 2 heavy chains and 4 light chains.

Post-translational modification

The N-terminus is blocked.

Sequence similarities

Contains 4 EF-hand domains.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   DomainRepeat
   Molecular functionMotor protein
Muscle protein
Myosin
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componentmyosin complex

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform MLC3 (identifier: P02605-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform MLC1 (identifier: P02604-1)

The sequence of this isoform can be found in the external entry P02604.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 150149Myosin light chain 3, skeletal muscle isoform
PRO_0000198705

Regions

Domain6 – 4136EF-hand 1
Domain44 – 7936EF-hand 2
Domain83 – 11836EF-hand 3
Domain118 – 15033EF-hand 4

Amino acid modifications

Modified residue21Blocked amino end (Ser)

Experimental info

Sequence conflict7 – 93EIN → QID AA sequence Ref.3
Sequence conflict741Q → QM in AAA48956. Ref.2
Sequence conflict1041Missing in AAA48956. Ref.2
Sequence conflict1281M → I in AAA48961. Ref.2

Secondary structure

............................ 150
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform MLC3 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 6F7BE6BA70055BE0

FASTA15016,710
        10         20         30         40         50         60 
MSFSPDEIND FKEAFLLFDR TGDAKITLSQ VGDIVRALGQ NPTNAEINKI LGNPSKEEMN 

        70         80         90        100        110        120 
AKKITFEEFL PMLQAAANNK DQGTFEDFVE GLRVFDKEGN GTVMGAELRH VLATLGEKMT 

       130        140        150 
EEEVEELMKG QEDSNGCINY EAFVKHIMSV 

« Hide

Isoform MLC1 [UniParc].

See P02604.

References

[1]"Molecular cloning and nucleotide sequences of the complementary DNAs to chicken skeletal muscle myosin two alkali light chain mRNAs."
Nabeshima Y., Fujii-Kuriyama Y., Muramatsu M., Ogata K.
Nucleic Acids Res. 10:6099-6110(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Alternative transcription and two modes of splicing results in two myosin light chains from one gene."
Nabeshima Y., Fujii-Kuriyama Y., Muramatsu M., Ogata K.
Nature 308:333-338(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Skeletal muscle.
[3]"Amino-acid sequence of the L-4 light chain of chicken skeletal-muscle myosin."
Maita T., Umegane T., Matsuda G.
Eur. J. Biochem. 114:45-49(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-150, BLOCKAGE OF N-TERMINUS.
Tissue: Skeletal muscle.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J00888 mRNA. Translation: AAA48961.1.
K02610, K02609 Genomic DNA. Translation: AAA48956.1.
PIRMOCHA2. I50384.
RefSeqNP_001038097.1. NM_001044632.1.
UniGeneGga.4835.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1MVWelectron microscopy70.00C/F/I/L/O/R10-150[»]
1O18electron microscopy70.00F/I/L/O/R10-150[»]
1O19electron microscopy70.00C/F/I/L/O/U10-150[»]
1O1Aelectron microscopy70.00C/F/I/L/O/R10-150[»]
1O1Belectron microscopy70.00C/F/I/L10-150[»]
1O1Celectron microscopy70.00C/F/I/L/R10-150[»]
1O1Delectron microscopy70.00C/F/I/L/O/R10-150[»]
1O1Eelectron microscopy70.00C/F/I/L/O/R10-150[»]
1O1Felectron microscopy70.00C/F/I/L10-150[»]
1O1Gelectron microscopy70.00C/F/I/L/O/R10-150[»]
2W4Aelectron microscopy35.00C6-150[»]
2W4Gelectron microscopy35.00C6-150[»]
2W4Helectron microscopy35.00C6-150[»]
ProteinModelPortalP02605.
SMRP02605. Positions 3-149.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP02605.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID396470.
KEGGgga:396470.

Organism-specific databases

CTD4632.

Phylogenomic databases

HOGENOMHOG000233018.
HOVERGENHBG012180.
KOK05738.

Family and domain databases

Gene3D1.10.238.10. 2 hits.
InterProIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
PfamPF13405. EF-hand_6. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTSM00054. EFh. 3 hits.
[Graphical view]
PROSITEPS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP02605.
NextBio20816509.

Entry information

Entry nameMLE3_CHICK
AccessionPrimary (citable) accession number: P02605
Secondary accession number(s): Q90896
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references