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P02604

- MLE1_CHICK

UniProt

P02604 - MLE1_CHICK

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Protein
Myosin light chain 1, skeletal muscle isoform
Gene
N/A
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. calcium ion binding Source: InterPro

GO - Biological processi

  1. muscle contraction Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin light chain 1, skeletal muscle isoform
Alternative name(s):
Alkali myosin light chain 1
Short name:
MLC-1
Myosin light chain 1f
Myosin light chain alkali 1
Short name:
Myosin light chain A1
Skeletal-muscle myosin L-1 light chain
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Chromosome 7

Subcellular locationi

GO - Cellular componenti

  1. myofibril Source: Ensembl
  2. myosin complex Source: UniProtKB-KW
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 192191Myosin light chain 1, skeletal muscle isoform
PRO_0000198704Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N,N,N-trimethylalanine Inferred

Post-translational modificationi

The N-terminus is blocked. N,N,N-trimethylalanine, found in other myosin light chains would not have been detected in the N-terminal tryptic peptide in Refs.1 and 2 because it would remain trimethylated and ninhydrin negative after hydrolysis.1 Publication

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiP02604.
PRIDEiP02604.

Interactioni

Subunit structurei

Myosin is a hexamer of 2 heavy chains and 4 light chains.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ALMmodel-C44-192[»]
1M8Qelectron microscopy70.00C/F/I/R51-192[»]
2MYSX-ray2.80C51-192[»]
ProteinModelPortaliP02604.
SMRiP02604. Positions 45-191.

Miscellaneous databases

EvolutionaryTraceiP02604.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini48 – 8336EF-hand 1
Add
BLAST
Domaini86 – 12136EF-hand 2
Add
BLAST
Domaini125 – 16036EF-hand 3
Add
BLAST
Domaini160 – 19233EF-hand 4
Add
BLAST

Sequence similaritiesi

Contains 4 EF-hand domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5126.
GeneTreeiENSGT00590000082921.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiP02604.
OrthoDBiEOG7HQN9N.
PhylomeDBiP02604.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
PROSITEiPS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform MLC1 (identifier: P02604-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAPKKDVKKP AAAAAPAPAP APAPAPAPAK PKEPAIDLKS IKIEFSKEQQ    50
DDFKEAFLLF DRTGDAKITL SQVGDIVRAL GQNPTNAEIN KILGNPSKEE 100
MNAKKITFEE FLPMLQAAAN NKDQGTFEDF VEGLRVFDKE GNGTVMGAEL 150
RHVLATLGEK MTEEEVEELM KGQEDSNGCI NYEAFVKHIM SV 192
Length:192
Mass (Da):20,899
Last modified:January 23, 2007 - v3
Checksum:i6F5594D5C67E63DD
GO
Isoform MLC3 (identifier: P02605-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry P02605.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:150
Mass (Da):16,710
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61D → E in AAA48955. 1 Publication
Sequence conflicti46 – 461S → F in AAA48960. 1 Publication
Sequence conflicti116 – 1161Q → QM in AAA48955. 1 Publication
Sequence conflicti146 – 1461Missing in AAA48955. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K02610, K02608, K02609 Genomic DNA. Translation: AAA48955.1.
J00887 mRNA. Translation: AAA48960.1.
PIRiA91284. MOCHLA.
UniGeneiGga.4835.

Genome annotation databases

EnsembliENSGALT00000004587; ENSGALP00000004578; ENSGALG00000002907. [P02604-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K02610 , K02608 , K02609 Genomic DNA. Translation: AAA48955.1 .
J00887 mRNA. Translation: AAA48960.1 .
PIRi A91284. MOCHLA.
UniGenei Gga.4835.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ALM model - C 44-192 [» ]
1M8Q electron microscopy 70.00 C/F/I/R 51-192 [» ]
2MYS X-ray 2.80 C 51-192 [» ]
ProteinModelPortali P02604.
SMRi P02604. Positions 45-191.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi P02604.
PRIDEi P02604.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSGALT00000004587 ; ENSGALP00000004578 ; ENSGALG00000002907 . [P02604-1 ]

Phylogenomic databases

eggNOGi COG5126.
GeneTreei ENSGT00590000082921.
HOGENOMi HOG000233018.
HOVERGENi HBG012180.
InParanoidi P02604.
OrthoDBi EOG7HQN9N.
PhylomeDBi P02604.

Miscellaneous databases

EvolutionaryTracei P02604.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
InterProi IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view ]
Pfami PF13405. EF-hand_6. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view ]
SMARTi SM00054. EFh. 3 hits.
[Graphical view ]
PROSITEi PS50222. EF_HAND_2. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Alternative transcription and two modes of splicing results in two myosin light chains from one gene."
    Nabeshima Y., Fujii-Kuriyama Y., Muramatsu M., Ogata K.
    Nature 308:333-338(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Skeletal muscle.
  2. "The primary structure of L-1 light chain of chicken fast skeletal muscle myosin and its genetic implication."
    Matsuda G., Maita T., Umegane T.
    FEBS Lett. 126:111-113(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 3-192.
    Tissue: Skeletal muscle.
  3. "Amino-acid sequence of the L-1 light chain of chicken fast skeletal-muscle myosin."
    Umegane T., Maita T., Matsuda G.
    Hoppe-Seyler's Z. Physiol. Chem. 363:1321-1330(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 3-192.
    Tissue: Skeletal muscle.
  4. "Molecular cloning and nucleotide sequences of the complementary DNAs to chicken skeletal muscle myosin two alkali light chain mRNAs."
    Nabeshima Y., Fujii-Kuriyama Y., Muramatsu M., Ogata K.
    Nucleic Acids Res. 10:6099-6110(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-192.
  5. "The widespread distribution of alpha-N-trimethylalanine as the N-terminal amino acid of light chains from vertebrate striated muscle myosins."
    Henry G.D., Trayer I.P., Brewer S., Levine B.A.
    Eur. J. Biochem. 148:75-82(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: METHYLATION AT ALA-2.
  6. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).

Entry informationi

Entry nameiMLE1_CHICK
AccessioniPrimary (citable) accession number: P02604
Secondary accession number(s): Q90895, Q9PWQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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