P02604 (MLE1_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Myosin light chain 1, skeletal muscle isoform Alternative name(s): Alkali myosin light chain 1 Short name=MLC-1 Myosin light chain 1f Myosin light chain alkali 1 Short name=Myosin light chain A1 Skeletal-muscle myosin L-1 light chain
Organism	Gallus gallus (Chicken) [Reference proteome]
Taxonomic identifier	9031 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Myosin is a hexamer of 2 heavy chains and 4 light chains.
Post-translational modification	The N-terminus is blocked. N,N,N-trimethylalanine, found in other myosin light chains would not have been detected in the N-terminal tryptic peptide in Refs.1 and 2 because it would remain trimethylated and ninhydrin negative after hydrolysis. Ref.5
Sequence similarities	Contains 4 EF-hand domains.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Repeat
Molecular function	Motor protein Muscle protein Myosin
PTM	Methylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	myosin complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform MLC1 (identifier: P02604-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform MLC3 (identifier: P02605-1)

The sequence of this isoform can be found in the external entry P02605.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 192	191	Myosin light chain 1, skeletal muscle isoform		PRO_0000198704
Regions
	Domain	48 – 83	36	EF-hand 1
	Domain	86 – 121	36	EF-hand 2
	Domain	125 – 160	36	EF-hand 3
	Domain	160 – 192	33	EF-hand 4
Amino acid modifications
	Modified residue	2	1	N,N,N-trimethylalanine Probable
Experimental info
	Sequence conflict	6	1	D → E in AAA48955. Ref.1
	Sequence conflict	46	1	S → F in AAA48960. Ref.4
	Sequence conflict	116	1	Q → QM in AAA48955. Ref.1
	Sequence conflict	146	1	Missing in AAA48955. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform MLC1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 6F5594D5C67E63DD	FASTA	192	20,899
	10 20 30 40 50 60 MAPKKDVKKP AAAAAPAPAP APAPAPAPAK PKEPAIDLKS IKIEFSKEQQ DDFKEAFLLF 70 80 90 100 110 120 DRTGDAKITL SQVGDIVRAL GQNPTNAEIN KILGNPSKEE MNAKKITFEE FLPMLQAAAN 130 140 150 160 170 180 NKDQGTFEDF VEGLRVFDKE GNGTVMGAEL RHVLATLGEK MTEEEVEELM KGQEDSNGCI 190 NYEAFVKHIM SV
	Isoform MLC3 [UniParc]. See P02605.		–	–

References

[1]	"Alternative transcription and two modes of splicing results in two myosin light chains from one gene." Nabeshima Y., Fujii-Kuriyama Y., Muramatsu M., Ogata K. Nature 308:333-338(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Skeletal muscle.
[2]	"The primary structure of L-1 light chain of chicken fast skeletal muscle myosin and its genetic implication." Matsuda G., Maita T., Umegane T. FEBS Lett. 126:111-113(1981) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 3-192. Tissue: Skeletal muscle.
[3]	"Amino-acid sequence of the L-1 light chain of chicken fast skeletal-muscle myosin." Umegane T., Maita T., Matsuda G. Hoppe-Seyler's Z. Physiol. Chem. 363:1321-1330(1982) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 3-192. Tissue: Skeletal muscle.
[4]	"Molecular cloning and nucleotide sequences of the complementary DNAs to chicken skeletal muscle myosin two alkali light chain mRNAs." Nabeshima Y., Fujii-Kuriyama Y., Muramatsu M., Ogata K. Nucleic Acids Res. 10:6099-6110(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-192.
[5]	"The widespread distribution of alpha-N-trimethylalanine as the N-terminal amino acid of light chains from vertebrate striated muscle myosins." Henry G.D., Trayer I.P., Brewer S., Levine B.A. Eur. J. Biochem. 148:75-82(1985) [PubMed] [Europe PMC] [Abstract] Cited for: METHYLATION AT ALA-2.
[6]	"Three-dimensional structure of myosin subfragment-1: a molecular motor." Rayment I., Rypniewski W.R., Schmidt-Base K., Smith R., Tomchick D.R., Benning M.M., Winkelmann D.A., Wesenberg G., Holden H.M. Science 261:50-58(1993) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

K02610, K02608, K02609 Genomic DNA. Translation: AAA48955.1.
J00887 mRNA. Translation: AAA48960.1.

IPI

IPI00578052.

PIR

MOCHLA. A91284.

UniGene

Gga.4835.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ALM	model	-	C	44-192	[»]
1M8Q	electron microscopy	70.00	C/F/I/R	51-192	[»]
2MYS	X-ray	2.80	C	44-192	[»]

ProteinModelPortal

P02604.

SMR

P02604. Positions 45-191.

ModBase

Search...

Proteomic databases

PaxDb

P02604.

PRIDE

P02604.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSGALT00000004587; ENSGALP00000004578; ENSGALG00000002907.

Phylogenomic databases

eggNOG

COG5126.

GeneTree

ENSGT00590000082921.

HOGENOM

HOG000233018.

HOVERGEN

HBG012180.

InParanoid

P02604.

OrthoDB

EOG4JM7R0.

Gene expression databases

ArrayExpress

P02604.

Family and domain databases

Gene3D

1.10.238.10. 2 hits.

InterPro

IPR011992. EF-hand-like_dom.
IPR002048. EF_hand_dom.
[Graphical view]

Pfam

PF13405. EF_hand_4. 1 hit.
PF13499. EF_hand_5. 1 hit.
[Graphical view]

SMART

SM00054. EFh. 3 hits.
[Graphical view]

PROSITE

PS50222. EF_HAND_2. 4 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P02604.

Entry information

Entry name

MLE1_CHICK

Accession

Primary (citable) accession number: P02604
Secondary accession number(s): Q90895, Q9PWQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 107 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program