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Protein

Myosin light chain 1, skeletal muscle isoform

Gene
N/A
Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

GO - Biological processi

  • muscle contraction Source: Ensembl
  • regulation of myosin II filament assembly Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Enzyme and pathway databases

ReactomeiREACT_303305. Striated Muscle Contraction.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin light chain 1, skeletal muscle isoform
Alternative name(s):
Alkali myosin light chain 1
Short name:
MLC-1
Myosin light chain 1f
Myosin light chain alkali 1
Short name:
Myosin light chain A1
Skeletal-muscle myosin L-1 light chain
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539 Componenti: Chromosome 7

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 192191Myosin light chain 1, skeletal muscle isoformPRO_0000198704Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N,N,N-trimethylalanine1 Publication

Post-translational modificationi

The N-terminus is blocked. N,N,N-trimethylalanine, found in other myosin light chains would not have been detected in the N-terminal tryptic peptide in Refs.1 and 2 because it would remain trimethylated and ninhydrin negative after hydrolysis.

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiP02604.
PRIDEiP02604.

Expressioni

Gene expression databases

ExpressionAtlasiP02604. baseline.

Interactioni

Subunit structurei

Myosin is a hexamer of 2 heavy chains and 4 light chains.

Protein-protein interaction databases

STRINGi9031.ENSGALP00000039262.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ALMmodel-C44-192[»]
1M8Qelectron microscopy70.00C/F/I/R51-192[»]
2MYSX-ray2.80C51-192[»]
ProteinModelPortaliP02604.
SMRiP02604. Positions 45-191.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02604.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini48 – 8336EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini86 – 12136EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini125 – 16036EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini160 – 19233EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5126.
GeneTreeiENSGT00590000082921.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiP02604.
OMAiTNAEINK.
OrthoDBiEOG7HQN9N.
PhylomeDBiP02604.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
PROSITEiPS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform MLC1 (identifier: P02604-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPKKDVKKP AAAAAPAPAP APAPAPAPAK PKEPAIDLKS IKIEFSKEQQ
60 70 80 90 100
DDFKEAFLLF DRTGDAKITL SQVGDIVRAL GQNPTNAEIN KILGNPSKEE
110 120 130 140 150
MNAKKITFEE FLPMLQAAAN NKDQGTFEDF VEGLRVFDKE GNGTVMGAEL
160 170 180 190
RHVLATLGEK MTEEEVEELM KGQEDSNGCI NYEAFVKHIM SV
Length:192
Mass (Da):20,899
Last modified:January 23, 2007 - v3
Checksum:i6F5594D5C67E63DD
GO
Isoform MLC3 (identifier: P02605-1) [UniParc]FASTAAdd to basket

The sequence of this isoform can be found in the external entry P02605.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:150
Mass (Da):16,710
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61D → E in AAA48955 (PubMed:6709041).Curated
Sequence conflicti46 – 461S → F in AAA48960 (PubMed:6128725).Curated
Sequence conflicti116 – 1161Q → QM in AAA48955 (PubMed:6709041).Curated
Sequence conflicti146 – 1461Missing in AAA48955 (PubMed:6709041).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02610, K02608, K02609 Genomic DNA. Translation: AAA48955.1.
J00887 mRNA. Translation: AAA48960.1.
PIRiA91284. MOCHLA.
UniGeneiGga.4835.

Genome annotation databases

EnsembliENSGALT00000004587; ENSGALP00000004578; ENSGALG00000002907. [P02604-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02610, K02608, K02609 Genomic DNA. Translation: AAA48955.1.
J00887 mRNA. Translation: AAA48960.1.
PIRiA91284. MOCHLA.
UniGeneiGga.4835.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ALMmodel-C44-192[»]
1M8Qelectron microscopy70.00C/F/I/R51-192[»]
2MYSX-ray2.80C51-192[»]
ProteinModelPortaliP02604.
SMRiP02604. Positions 45-191.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000039262.

Proteomic databases

PaxDbiP02604.
PRIDEiP02604.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000004587; ENSGALP00000004578; ENSGALG00000002907. [P02604-1]

Phylogenomic databases

eggNOGiCOG5126.
GeneTreeiENSGT00590000082921.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiP02604.
OMAiTNAEINK.
OrthoDBiEOG7HQN9N.
PhylomeDBiP02604.

Enzyme and pathway databases

ReactomeiREACT_303305. Striated Muscle Contraction.

Miscellaneous databases

EvolutionaryTraceiP02604.

Gene expression databases

ExpressionAtlasiP02604. baseline.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
PROSITEiPS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Alternative transcription and two modes of splicing results in two myosin light chains from one gene."
    Nabeshima Y., Fujii-Kuriyama Y., Muramatsu M., Ogata K.
    Nature 308:333-338(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Skeletal muscle.
  2. "The primary structure of L-1 light chain of chicken fast skeletal muscle myosin and its genetic implication."
    Matsuda G., Maita T., Umegane T.
    FEBS Lett. 126:111-113(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 3-192.
    Tissue: Skeletal muscle.
  3. "Amino-acid sequence of the L-1 light chain of chicken fast skeletal-muscle myosin."
    Umegane T., Maita T., Matsuda G.
    Hoppe-Seyler's Z. Physiol. Chem. 363:1321-1330(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 3-192.
    Tissue: Skeletal muscle.
  4. "Molecular cloning and nucleotide sequences of the complementary DNAs to chicken skeletal muscle myosin two alkali light chain mRNAs."
    Nabeshima Y., Fujii-Kuriyama Y., Muramatsu M., Ogata K.
    Nucleic Acids Res. 10:6099-6110(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-192.
  5. "The widespread distribution of alpha-N-trimethylalanine as the N-terminal amino acid of light chains from vertebrate striated muscle myosins."
    Henry G.D., Trayer I.P., Brewer S., Levine B.A.
    Eur. J. Biochem. 148:75-82(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: METHYLATION AT ALA-2.
  6. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).

Entry informationi

Entry nameiMLE1_CHICK
AccessioniPrimary (citable) accession number: P02604
Secondary accession number(s): Q90895, Q9PWQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.