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P02604

- MLE1_CHICK

UniProt

P02604 - MLE1_CHICK

Protein

Myosin light chain 1, skeletal muscle isoform

Gene
N/A
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro

    GO - Biological processi

    1. muscle contraction Source: Ensembl

    Keywords - Molecular functioni

    Motor protein, Muscle protein, Myosin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myosin light chain 1, skeletal muscle isoform
    Alternative name(s):
    Alkali myosin light chain 1
    Short name:
    MLC-1
    Myosin light chain 1f
    Myosin light chain alkali 1
    Short name:
    Myosin light chain A1
    Skeletal-muscle myosin L-1 light chain
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Chromosome 7

    Subcellular locationi

    GO - Cellular componenti

    1. myofibril Source: Ensembl
    2. myosin complex Source: UniProtKB-KW

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 192191Myosin light chain 1, skeletal muscle isoformPRO_0000198704Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N,N,N-trimethylalanine1 Publication

    Post-translational modificationi

    The N-terminus is blocked. N,N,N-trimethylalanine, found in other myosin light chains would not have been detected in the N-terminal tryptic peptide in Refs.1 and 2 because it would remain trimethylated and ninhydrin negative after hydrolysis.

    Keywords - PTMi

    Methylation

    Proteomic databases

    PaxDbiP02604.
    PRIDEiP02604.

    Interactioni

    Subunit structurei

    Myosin is a hexamer of 2 heavy chains and 4 light chains.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ALMmodel-C44-192[»]
    1M8Qelectron microscopy70.00C/F/I/R51-192[»]
    2MYSX-ray2.80C51-192[»]
    ProteinModelPortaliP02604.
    SMRiP02604. Positions 45-191.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP02604.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini48 – 8336EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini86 – 12136EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini125 – 16036EF-hand 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini160 – 19233EF-hand 4PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 4 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5126.
    GeneTreeiENSGT00590000082921.
    HOGENOMiHOG000233018.
    HOVERGENiHBG012180.
    InParanoidiP02604.
    OrthoDBiEOG7HQN9N.
    PhylomeDBiP02604.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR002048. EF_hand_dom.
    [Graphical view]
    PfamiPF13405. EF-hand_6. 1 hit.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view]
    SMARTiSM00054. EFh. 3 hits.
    [Graphical view]
    PROSITEiPS50222. EF_HAND_2. 4 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform MLC1 (identifier: P02604-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAPKKDVKKP AAAAAPAPAP APAPAPAPAK PKEPAIDLKS IKIEFSKEQQ    50
    DDFKEAFLLF DRTGDAKITL SQVGDIVRAL GQNPTNAEIN KILGNPSKEE 100
    MNAKKITFEE FLPMLQAAAN NKDQGTFEDF VEGLRVFDKE GNGTVMGAEL 150
    RHVLATLGEK MTEEEVEELM KGQEDSNGCI NYEAFVKHIM SV 192
    Length:192
    Mass (Da):20,899
    Last modified:January 23, 2007 - v3
    Checksum:i6F5594D5C67E63DD
    GO
    Isoform MLC3 (identifier: P02605-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry P02605.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:150
    Mass (Da):16,710
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti6 – 61D → E in AAA48955. (PubMed:6709041)Curated
    Sequence conflicti46 – 461S → F in AAA48960. (PubMed:6128725)Curated
    Sequence conflicti116 – 1161Q → QM in AAA48955. (PubMed:6709041)Curated
    Sequence conflicti146 – 1461Missing in AAA48955. (PubMed:6709041)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02610, K02608, K02609 Genomic DNA. Translation: AAA48955.1.
    J00887 mRNA. Translation: AAA48960.1.
    PIRiA91284. MOCHLA.
    UniGeneiGga.4835.

    Genome annotation databases

    EnsembliENSGALT00000004587; ENSGALP00000004578; ENSGALG00000002907. [P02604-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02610 , K02608 , K02609 Genomic DNA. Translation: AAA48955.1 .
    J00887 mRNA. Translation: AAA48960.1 .
    PIRi A91284. MOCHLA.
    UniGenei Gga.4835.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ALM model - C 44-192 [» ]
    1M8Q electron microscopy 70.00 C/F/I/R 51-192 [» ]
    2MYS X-ray 2.80 C 51-192 [» ]
    ProteinModelPortali P02604.
    SMRi P02604. Positions 45-191.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P02604.
    PRIDEi P02604.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSGALT00000004587 ; ENSGALP00000004578 ; ENSGALG00000002907 . [P02604-1 ]

    Phylogenomic databases

    eggNOGi COG5126.
    GeneTreei ENSGT00590000082921.
    HOGENOMi HOG000233018.
    HOVERGENi HBG012180.
    InParanoidi P02604.
    OrthoDBi EOG7HQN9N.
    PhylomeDBi P02604.

    Miscellaneous databases

    EvolutionaryTracei P02604.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR002048. EF_hand_dom.
    [Graphical view ]
    Pfami PF13405. EF-hand_6. 1 hit.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view ]
    SMARTi SM00054. EFh. 3 hits.
    [Graphical view ]
    PROSITEi PS50222. EF_HAND_2. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Alternative transcription and two modes of splicing results in two myosin light chains from one gene."
      Nabeshima Y., Fujii-Kuriyama Y., Muramatsu M., Ogata K.
      Nature 308:333-338(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Skeletal muscle.
    2. "The primary structure of L-1 light chain of chicken fast skeletal muscle myosin and its genetic implication."
      Matsuda G., Maita T., Umegane T.
      FEBS Lett. 126:111-113(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 3-192.
      Tissue: Skeletal muscle.
    3. "Amino-acid sequence of the L-1 light chain of chicken fast skeletal-muscle myosin."
      Umegane T., Maita T., Matsuda G.
      Hoppe-Seyler's Z. Physiol. Chem. 363:1321-1330(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 3-192.
      Tissue: Skeletal muscle.
    4. "Molecular cloning and nucleotide sequences of the complementary DNAs to chicken skeletal muscle myosin two alkali light chain mRNAs."
      Nabeshima Y., Fujii-Kuriyama Y., Muramatsu M., Ogata K.
      Nucleic Acids Res. 10:6099-6110(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-192.
    5. "The widespread distribution of alpha-N-trimethylalanine as the N-terminal amino acid of light chains from vertebrate striated muscle myosins."
      Henry G.D., Trayer I.P., Brewer S., Levine B.A.
      Eur. J. Biochem. 148:75-82(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: METHYLATION AT ALA-2.
    6. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).

    Entry informationi

    Entry nameiMLE1_CHICK
    AccessioniPrimary (citable) accession number: P02604
    Secondary accession number(s): Q90895, Q9PWQ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 116 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3