Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Troponin C, skeletal muscle

Gene

TNNC2

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi28 – 39121Add
BLAST
Calcium bindingi64 – 75122Add
BLAST
Calcium bindingi104 – 115123Add
BLAST
Calcium bindingi140 – 151124Add
BLAST

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • troponin T binding Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Muscle protein

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Troponin C, skeletal muscle
Gene namesi
Name:TNNC2
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 160159Troponin C, skeletal musclePRO_0000073706Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonine1 Publication

Keywords - PTMi

Acetylation

Interactioni

GO - Molecular functioni

  • troponin T binding Source: UniProtKB

Protein-protein interaction databases

BioGridi1172361. 2 interactions.
IntActiP02586. 1 interaction.
MINTiMINT-1534481.
STRINGi9986.ENSOCUP00000020944.

Structurei

Secondary structure

1
160
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 118Combined sources
Helixi14 – 2714Combined sources
Beta strandi32 – 354Combined sources
Helixi37 – 4610Combined sources
Helixi53 – 6311Combined sources
Beta strandi64 – 663Combined sources
Beta strandi69 – 724Combined sources
Helixi73 – 10331Combined sources
Beta strandi108 – 1125Combined sources
Helixi113 – 12311Combined sources
Helixi129 – 13911Combined sources
Beta strandi144 – 1474Combined sources
Helixi149 – 1579Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A2XX-ray2.30A2-160[»]
1TCFX-ray1.90A2-160[»]
1TN4X-ray1.95A2-160[»]
1TNCmodel-A15-160[»]
2TN4X-ray2.00A2-160[»]
ProteinModelPortaliP02586.
SMRiP02586. Positions 3-160.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02586.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 5036EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini51 – 8636EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini91 – 12636EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini127 – 16034EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the troponin C family.Curated
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0027. Eukaryota.
COG5126. LUCA.
HOVERGENiHBG012180.
InParanoidiP02586.
KOiK12042.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02586-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDQQAEARS YLSEEMIAEF KAAFDMFDAD GGGDISVKEL GTVMRMLGQT
60 70 80 90 100
PTKEELDAII EEVDEDGSGT IDFEEFLVMM VRQMKEDAKG KSEEELAECF
110 120 130 140 150
RIFDRNADGY IDAEELAEIF RASGEHVTDE EIESLMKDGD KNNDGRIDFD
160
EFLKMMEGVQ
Length:160
Mass (Da):18,096
Last modified:January 23, 2007 - v2
Checksum:i81FED9E732A0F902
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 32TD → DT AA sequence (PubMed:893419).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03462 mRNA. Translation: AAA31481.1.
Y00760 mRNA. Translation: CAA68729.1.
PIRiA28442. TPRBCS.
RefSeqiNP_001076114.1. NM_001082645.1.
UniGeneiOcu.6659.

Genome annotation databases

GeneIDi100009341.
KEGGiocu:100009341.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03462 mRNA. Translation: AAA31481.1.
Y00760 mRNA. Translation: CAA68729.1.
PIRiA28442. TPRBCS.
RefSeqiNP_001076114.1. NM_001082645.1.
UniGeneiOcu.6659.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A2XX-ray2.30A2-160[»]
1TCFX-ray1.90A2-160[»]
1TN4X-ray1.95A2-160[»]
1TNCmodel-A15-160[»]
2TN4X-ray2.00A2-160[»]
ProteinModelPortaliP02586.
SMRiP02586. Positions 3-160.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi1172361. 2 interactions.
IntActiP02586. 1 interaction.
MINTiMINT-1534481.
STRINGi9986.ENSOCUP00000020944.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100009341.
KEGGiocu:100009341.

Organism-specific databases

CTDi7125.

Phylogenomic databases

eggNOGiKOG0027. Eukaryota.
COG5126. LUCA.
HOVERGENiHBG012180.
InParanoidiP02586.
KOiK12042.

Miscellaneous databases

EvolutionaryTraceiP02586.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and sequence of a cDNA clone for rabbit fast skeletal muscle troponin C. Homology with calmodulin and parvalbumin."
    Zot A.S., Potter J.D., Strauss W.L.
    J. Biol. Chem. 262:15418-15421(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning, sequencing and expression of a full-length rabbit fast skeletal troponin-C cDNA."
    Chen Q., Taljanidisz J., Satyapriya S., Tao T., Gergely J.
    FEBS Lett. 228:22-26(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Determination of the amino acid sequence of troponon C from rabbit skeletal muscle."
    Collins J.H., Greaser M.L., Potter J.D., Horn M.J.
    J. Biol. Chem. 252:6456-6462(1977) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-160, ACETYLATION AT THR-2.
  4. "The predicted structure of the calcium-binding component of troponin."
    Kretsinger R.H., Barry C.D.
    Biochim. Biophys. Acta 405:40-52(1975) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING.
  5. "Solution structure of a polypeptide dimer comprising the fourth Ca(2+)-binding site of troponin C by nuclear magnetic resonance spectroscopy."
    Kay L.E., Forman-Kay J.D., McCubbin W.D., Kay C.M.
    Biochemistry 30:4323-4333(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 122-160.
  6. "Structures of four Ca2+-bound troponin C at 2.0-A resolution: further insights into the Ca2+-switch in the calmodulin superfamily."
    Houdusse A., Love M.L., Dominguez R., Grabarek Z., Cohen C.
    Structure 5:1695-1711(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
  7. "Conformational variation of calcium-bound troponin C."
    Soman J., Tao T., Phillips G.N. Jr.
    Proteins 37:510-511(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
  8. "Crystal structure of troponin C in complex with troponin I fragment at 2.3-A resolution."
    Vassylyev D.G., Takeda S., Wakatsuki S., Maeda K., Maeda Y.
    Proc. Natl. Acad. Sci. U.S.A. 95:4847-4852(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF COMPLEX WITH TNI.

Entry informationi

Entry nameiTNNC2_RABIT
AccessioniPrimary (citable) accession number: P02586
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: March 16, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Skeletal muscle troponin C binds four calcium ions.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.