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Protein

Profilin-1

Gene

PFN1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiR-BTA-376176. Signaling by Robo receptor.
R-BTA-4086400. PCP/CE pathway.
R-BTA-5663220. RHO GTPases Activate Formins.

Names & Taxonomyi

Protein namesi
Recommended name:
Profilin-1
Alternative name(s):
Profilin I
Gene namesi
Name:PFN1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 19

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 140139Profilin-1PRO_0000199570Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine2 Publications
Modified residuei28 – 281PhosphoserineBy similarity
Cross-linki54 – 54Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei57 – 571PhosphoserineBy similarity
Modified residuei108 – 1081N6-acetyllysineBy similarity
Modified residuei129 – 1291PhosphotyrosineBy similarity
Modified residuei138 – 1381Phosphoserine; by ROCK1By similarity

Post-translational modificationi

Phosphorylation at Ser-138 reduces its affinity for G-actin and blocks its interaction with HTT, reducing its ability to inhibit androgen receptor (AR) and HTT aggregation.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP02584.
PeptideAtlasiP02584.
PRIDEiP02584.

PTM databases

iPTMnetiP02584.

Interactioni

Subunit structurei

Interacts with VASP. Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio. Found in a complex with XPO6, Ran, ACTB and PFN1 (By similarity). Interacts with HTT (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi170522. 2 interactions.
DIPiDIP-17037N.
IntActiP02584. 1 interaction.
MINTiMINT-1512803.
STRINGi9913.ENSBTAP00000006465.

Structurei

Secondary structure

1
140
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 128Combined sources
Beta strandi15 – 2410Combined sources
Beta strandi26 – 283Combined sources
Beta strandi30 – 345Combined sources
Helixi40 – 423Combined sources
Helixi45 – 517Combined sources
Helixi58 – 625Combined sources
Beta strandi64 – 663Combined sources
Beta strandi69 – 768Combined sources
Turni81 – 833Combined sources
Beta strandi85 – 906Combined sources
Beta strandi100 – 1056Combined sources
Beta strandi107 – 1159Combined sources
Helixi121 – 13717Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HLUX-ray2.65P2-140[»]
1PNEX-ray2.00A2-140[»]
2BTFX-ray2.55P2-140[»]
3U4LX-ray2.40P2-140[»]
3UB5X-ray2.20P2-140[»]
ProteinModelPortaliP02584.
SMRiP02584. Positions 2-140.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02584.

Family & Domainsi

Sequence similaritiesi

Belongs to the profilin family.Curated

Phylogenomic databases

eggNOGiKOG1755. Eukaryota.
ENOG41126PD. LUCA.
GeneTreeiENSGT00390000010143.
HOGENOMiHOG000171592.
HOVERGENiHBG053683.
InParanoidiP02584.
KOiK05759.
OMAiHLRRAQY.
OrthoDBiEOG7JMGGT.
TreeFamiTF331744.

Family and domain databases

InterProiIPR005455. PFN.
IPR005454. Profilin1/2/3_vertebrate.
IPR027310. Profilin_CS.
[Graphical view]
PfamiPF00235. Profilin. 1 hit.
[Graphical view]
PRINTSiPR00392. PROFILIN.
PR01639. PROFILINMAML.
SMARTiSM00392. PROF. 1 hit.
[Graphical view]
SUPFAMiSSF55770. SSF55770. 1 hit.
PROSITEiPS00414. PROFILIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02584-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGWNAYIDN LMADGTCQDA AIVGYKDSPS VWAAVPGKTF VNITPAEVGI
60 70 80 90 100
LVGKDRSSFF VNGLTLGGQK CSVIRDSLLQ DGEFTMDLRT KSTGGAPTFN
110 120 130 140
ITVTMTAKTL VLLMGKEGVH GGMINKKCYE MASHLRRSQY
Length:140
Mass (Da):15,057
Last modified:January 23, 2007 - v2
Checksum:iE56BFBB5BBB016E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021078 mRNA. Translation: AAX09095.1.
BC102240 mRNA. Translation: AAI02241.1.
PIRiS00308. FABO.
RefSeqiNP_001015592.1. NM_001015592.1.
UniGeneiBt.23180.

Genome annotation databases

EnsembliENSBTAT00000006465; ENSBTAP00000006465; ENSBTAG00000004915.
GeneIDi513895.
KEGGibta:513895.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021078 mRNA. Translation: AAX09095.1.
BC102240 mRNA. Translation: AAI02241.1.
PIRiS00308. FABO.
RefSeqiNP_001015592.1. NM_001015592.1.
UniGeneiBt.23180.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HLUX-ray2.65P2-140[»]
1PNEX-ray2.00A2-140[»]
2BTFX-ray2.55P2-140[»]
3U4LX-ray2.40P2-140[»]
3UB5X-ray2.20P2-140[»]
ProteinModelPortaliP02584.
SMRiP02584. Positions 2-140.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi170522. 2 interactions.
DIPiDIP-17037N.
IntActiP02584. 1 interaction.
MINTiMINT-1512803.
STRINGi9913.ENSBTAP00000006465.

PTM databases

iPTMnetiP02584.

Proteomic databases

PaxDbiP02584.
PeptideAtlasiP02584.
PRIDEiP02584.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000006465; ENSBTAP00000006465; ENSBTAG00000004915.
GeneIDi513895.
KEGGibta:513895.

Organism-specific databases

CTDi5216.

Phylogenomic databases

eggNOGiKOG1755. Eukaryota.
ENOG41126PD. LUCA.
GeneTreeiENSGT00390000010143.
HOGENOMiHOG000171592.
HOVERGENiHBG053683.
InParanoidiP02584.
KOiK05759.
OMAiHLRRAQY.
OrthoDBiEOG7JMGGT.
TreeFamiTF331744.

Enzyme and pathway databases

ReactomeiR-BTA-376176. Signaling by Robo receptor.
R-BTA-4086400. PCP/CE pathway.
R-BTA-5663220. RHO GTPases Activate Formins.

Miscellaneous databases

EvolutionaryTraceiP02584.

Family and domain databases

InterProiIPR005455. PFN.
IPR005454. Profilin1/2/3_vertebrate.
IPR027310. Profilin_CS.
[Graphical view]
PfamiPF00235. Profilin. 1 hit.
[Graphical view]
PRINTSiPR00392. PROFILIN.
PR01639. PROFILINMAML.
SMARTiSM00392. PROF. 1 hit.
[Graphical view]
SUPFAMiSSF55770. SSF55770. 1 hit.
PROSITEiPS00414. PROFILIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.
  3. "The amino acid sequence of profilin from calf spleen."
    Nystroem L.-E., Lindberg U., Kendrick-Jones J., Jakes R.
    FEBS Lett. 101:161-165(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-140, ACETYLATION AT ALA-2.
    Tissue: Spleen.
  4. "The primary structure of human platelet profilin: reinvestigation of the calf spleen profilin sequence."
    Ampe C., Markey F., Lindberg U., Vandekerckhove J.
    FEBS Lett. 228:17-21(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  5. Cited for: X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS) OF COMPLEX WITH BETA-ACTIN.
  6. "The structure of an open state of beta-actin at 2.65-A resolution."
    Chik J.K., Lindberg U., Schutt C.E.
    J. Mol. Biol. 263:607-623(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) OF COMPLEX WITH BETA-ACTIN.
  7. "Crystallization and structure determination of bovine profilin at 2.0-A resolution."
    Cedergren-Zeppezauer E.S., Goonesekere N.C., Rozycki M.D., Myslik J.C., Dauter Z., Lindberg U., Schutt C.E.
    J. Mol. Biol. 240:459-475(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Entry informationi

Entry nameiPROF1_BOVIN
AccessioniPrimary (citable) accession number: P02584
Secondary accession number(s): Q3ZCH4, Q5E942
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.