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Protein

Profilin-1

Gene

PFN1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding

Enzyme and pathway databases

ReactomeiR-BTA-376176. Signaling by Robo receptor.
R-BTA-4086400. PCP/CE pathway.
R-BTA-5663220. RHO GTPases Activate Formins.

Names & Taxonomyi

Protein namesi
Recommended name:
Profilin-1
Alternative name(s):
Profilin I
Gene namesi
Name:PFN1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 19

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001995702 – 140Profilin-1Add BLAST139

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine2 Publications1
Modified residuei28PhosphoserineBy similarity1
Cross-linki54Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei57PhosphoserineBy similarity1
Modified residuei108N6-acetyllysineBy similarity1
Modified residuei129PhosphotyrosineBy similarity1
Modified residuei138Phosphoserine; by ROCK1By similarity1

Post-translational modificationi

Phosphorylation at Ser-138 reduces its affinity for G-actin and blocks its interaction with HTT, reducing its ability to inhibit androgen receptor (AR) and HTT aggregation.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP02584.
PeptideAtlasiP02584.
PRIDEiP02584.

PTM databases

iPTMnetiP02584.

Expressioni

Gene expression databases

BgeeiENSBTAG00000004915.

Interactioni

Subunit structurei

Interacts with VASP. Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio. Found in a complex with XPO6, Ran, ACTB and PFN1 (By similarity). Interacts with HTT (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi170522. 2 interactors.
DIPiDIP-17037N.
IntActiP02584. 1 interactor.
MINTiMINT-1512803.
STRINGi9913.ENSBTAP00000006465.

Structurei

Secondary structure

1140
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 12Combined sources8
Beta strandi15 – 24Combined sources10
Beta strandi26 – 28Combined sources3
Beta strandi30 – 34Combined sources5
Helixi40 – 42Combined sources3
Helixi45 – 51Combined sources7
Helixi58 – 62Combined sources5
Beta strandi64 – 66Combined sources3
Beta strandi69 – 76Combined sources8
Turni81 – 83Combined sources3
Beta strandi85 – 90Combined sources6
Beta strandi100 – 105Combined sources6
Beta strandi107 – 115Combined sources9
Helixi121 – 137Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HLUX-ray2.65P2-140[»]
1PNEX-ray2.00A2-140[»]
2BTFX-ray2.55P2-140[»]
3U4LX-ray2.40P2-140[»]
3UB5X-ray2.20P2-140[»]
ProteinModelPortaliP02584.
SMRiP02584.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02584.

Family & Domainsi

Sequence similaritiesi

Belongs to the profilin family.Curated

Phylogenomic databases

eggNOGiKOG1755. Eukaryota.
ENOG41126PD. LUCA.
GeneTreeiENSGT00390000010143.
HOGENOMiHOG000171592.
HOVERGENiHBG053683.
InParanoidiP02584.
KOiK05759.
OMAiHLRRAQY.
OrthoDBiEOG091G0TWH.
TreeFamiTF331744.

Family and domain databases

CDDicd00148. PROF. 1 hit.
InterProiView protein in InterPro
IPR005455. PFN.
IPR005454. Profilin1/2/3_vertebrate.
IPR027310. Profilin_CS.
PfamiView protein in Pfam
PF00235. Profilin. 1 hit.
PRINTSiPR00392. PROFILIN.
PR01639. PROFILINMAML.
SMARTiView protein in SMART
SM00392. PROF. 1 hit.
SUPFAMiSSF55770. SSF55770. 1 hit.
PROSITEiView protein in PROSITE
PS00414. PROFILIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02584-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGWNAYIDN LMADGTCQDA AIVGYKDSPS VWAAVPGKTF VNITPAEVGI
60 70 80 90 100
LVGKDRSSFF VNGLTLGGQK CSVIRDSLLQ DGEFTMDLRT KSTGGAPTFN
110 120 130 140
ITVTMTAKTL VLLMGKEGVH GGMINKKCYE MASHLRRSQY
Length:140
Mass (Da):15,057
Last modified:January 23, 2007 - v2
Checksum:iE56BFBB5BBB016E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021078 mRNA. Translation: AAX09095.1.
BC102240 mRNA. Translation: AAI02241.1.
PIRiS00308. FABO.
RefSeqiNP_001015592.1. NM_001015592.1.
UniGeneiBt.23180.

Genome annotation databases

EnsembliENSBTAT00000006465; ENSBTAP00000006465; ENSBTAG00000004915.
GeneIDi513895.
KEGGibta:513895.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPROF1_BOVIN
AccessioniPrimary (citable) accession number: P02584
Secondary accession number(s): Q3ZCH4, Q5E942
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: March 15, 2017
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families