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Protein

Myosin-4

Gene

unc-54

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Muscle contraction.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi174 – 1818ATPBy similarity

GO - Molecular functioni

  • actin filament binding Source: WormBase
  • ATP binding Source: UniProtKB-KW
  • microfilament motor activity Source: WormBase
  • structural constituent of muscle Source: WormBase

GO - Biological processi

  • inositol lipid-mediated signaling Source: WormBase
  • locomotion Source: WormBase
  • muscle contraction Source: WormBase
  • oviposition Source: WormBase
  • pharyngeal pumping Source: WormBase
  • skeletal muscle myosin thick filament assembly Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Keywords - Ligandi

Actin-binding, ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-4
Alternative name(s):
Myosin heavy chain B
Short name:
MHC B
Uncoordinated protein 54
Gene namesi
Name:unc-54
Synonyms:myo-4
ORF Names:F11C3.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiF11C3.3; CE09349; WBGene00006789; unc-54.

Subcellular locationi

GO - Cellular componenti

  • muscle myosin complex Source: WormBase
  • striated muscle myosin thick filament Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Thick filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19631963Myosin-4PRO_0000123383Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei125 – 1251N6,N6,N6-trimethyllysineSequence analysis

Keywords - PTMi

Methylation

Proteomic databases

EPDiP02566.
PaxDbiP02566.
PRIDEiP02566.

PTM databases

iPTMnetiP02566.

Interactioni

Subunit structurei

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with itr-1 (via c-terminal coiled coil domain).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
unc-45G5EG623EBI-329238,EBI-6675165

GO - Molecular functioni

  • actin filament binding Source: WormBase

Protein-protein interaction databases

BioGridi56866. 5 interactions.
DIPiDIP-26548N.
IntActiP02566. 6 interactions.
MINTiMINT-231086.
STRINGi6239.F11C3.3.2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 787707Myosin motorAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni662 – 68423Actin-bindingAdd
BLAST
Regioni766 – 78015Actin-bindingAdd
BLAST
Regioni848 – 1161314Alpha-helical tailpiece (S2)Add
BLAST
Regioni1162 – 1963802Light meromyosin (LMM)Add
BLAST
Regioni1162 – 117312HingeAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili848 – 19631116Sequence analysisAdd
BLAST

Domaini

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).Curated

Sequence similaritiesi

Contains 1 myosin motor domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0161. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00760000118919.
HOGENOMiHOG000173959.
InParanoidiP02566.
KOiK10352.
OMAiDIKRSCW.

Family and domain databases

Gene3Di4.10.270.10. 1 hit.
InterProiIPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P02566-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHEKDPGWQ YLRRTREQVL EDQSKPYDSK KNVWIPDPEE GYLAGEITAT
60 70 80 90 100
KGDQVTIVTA RGNEVTLKKE LVQEMNPPKF EKTEDMSNLS FLNDASVLHN
110 120 130 140 150
LRSRYAAMLI YTYSGLFCVV INPYKRLPIY TDSCARMFMG KRKTEMPPHL
160 170 180 190 200
FAVSDEAYRN MLQDHENQSM LITGESGAGK TENTKKVICY FAAVGASQQE
210 220 230 240 250
GGAEVDPNKK KVTLEDQIVQ TNPVLEAFGN AKTVRNNNSS RFGKFIRIHF
260 270 280 290 300
NKHGRLASCD IEHYLLEKSR VIRQAPGERC YHIFYQIYSD FRPELKKELL
310 320 330 340 350
LDLPIKDYWF VAQAELIIDG IDDVEEFQLT DEAFDILNFS AVEKQDCYRL
360 370 380 390 400
MSAHMHMGNM KFKQRPREEQ AEPDGTDEAE KASNMYGIGC EEFLKALTKP
410 420 430 440 450
RVKVGTEWVS KGQNCEQVNW AVGAMAKGLY SRVFNWLVKK CNLTLDQKGI
460 470 480 490 500
DRDYFIGVLD IAGFEIFDFN SFEQLWINFV NEKLQQFFNH HMFVLEQEEY
510 520 530 540 550
AREGIQWVFI DFGLDLQACI ELIEKPLGII SMLDEECIVP KATDLTLASK
560 570 580 590 600
LVDQHLGKHP NFEKPKPPKG KQGEAHFAMR HYAGTVRYNC LNWLEKNKDP
610 620 630 640 650
LNDTVVSAMK QSKGNDLLVE IWQDYTTQEE AAAKAKEGGG GGKKKGKSGS
660 670 680 690 700
FMTVSMLYRE SLNNLMTMLN KTHPHFIRCI IPNEKKQSGM IDAALVLNQL
710 720 730 740 750
TCNGVLEGIR ICRKGFPNRT LHPDFVQRYA ILAAKEAKSD DDKKKCAEAI
760 770 780 790 800
MSKLVNDGSL SEEMFRIGLT KVFFKAGVLA HLEDIRDEKL ATILTGFQSQ
810 820 830 840 850
IRWHLGLKDR KRRMEQRAGL LIVQRNVRSW CTLRTWEWFK LYGKVKPMLK
860 870 880 890 900
AGKEAEELEK INDKVKALED SLAKEEKLRK ELEESSAKLV EEKTSLFTNL
910 920 930 940 950
ESTKTQLSDA EERLAKLEAQ QKDASKQLSE LNDQLADNED RTADVQRAKK
960 970 980 990 1000
KIEAEVEALK KQIQDLEMSL RKAESEKQSK DHQIRSLQDE MQQQDEAIAK
1010 1020 1030 1040 1050
LNKEKKHQEE INRKLMEDLQ SEEDKGNHQN KVKAKLEQTL DDLEDSLERE
1060 1070 1080 1090 1100
KRARADLDKQ KRKVEGELKI AQENIDESGR QRHDLENNLK KKESELHSVS
1110 1120 1130 1140 1150
SRLEDEQALV SKLQRQIKDG QSRISELEEE LENERQSRSK ADRAKSDLQR
1160 1170 1180 1190 1200
ELEELGEKLD EQGGATAAQV EVNKKREAEL AKLRRDLEEA NMNHENQLGG
1210 1220 1230 1240 1250
LRKKHTDAVA ELTDQLDQLN KAKAKVEKDK AQAVRDAEDL AAQLDQETSG
1260 1270 1280 1290 1300
KLNNEKLAKQ FELQLTELQS KADEQSRQLQ DFTSLKGRLH SENGDLVRQL
1310 1320 1330 1340 1350
EDAESQVNQL TRLKSQLTSQ LEEARRTADE EARERQTVAA QAKNYQHEAE
1360 1370 1380 1390 1400
QLQESLEEEI EGKNEILRQL SKANADIQQW KARFEGEGLL KADELEDAKR
1410 1420 1430 1440 1450
RQAQKINELQ EALDAANSKN ASLEKTKSRL VGDLDDAQVD VERANGVASA
1460 1470 1480 1490 1500
LEKKQKGFDK IIDEWRKKTD DLAAELDGAQ RDLRNTSTDL FKAKNAQEEL
1510 1520 1530 1540 1550
AEVVEGLRRE NKSLSQEIKD LTDQLGEGGR SVHEMQKIIR RLEIEKEELQ
1560 1570 1580 1590 1600
HALDEAEAAL EAEESKVLRA QVEVSQIRSE IEKRIQEKEE EFENTRKNHA
1610 1620 1630 1640 1650
RALESMQASL ETEAKGKAEL LRIKKKLEGD INELEIALDH ANKANADAQK
1660 1670 1680 1690 1700
NLKRYQEQVR ELQLQVEEEQ RNGADTREQF FNAEKRATLL QSEKEELLVA
1710 1720 1730 1740 1750
NEAAERARKQ AEYEAADARD QANEANAQVS SLTSAKRKLE GEIQAIHADL
1760 1770 1780 1790 1800
DETLNEYKAA EERSKKAIAD ATRLAEELRQ EQEHSQHVDR LRKGLEQQLK
1810 1820 1830 1840 1850
EIQVRLDEAE AAALKGGKKV IAKLEQRVRE LESELDGEQR RFQDANKNLG
1860 1870 1880 1890 1900
RADRRVRELQ FQVDEDKKNF ERLQDLIDKL QQKLKTQKKQ VEEAEELANL
1910 1920 1930 1940 1950
NLQKYKQLTH QLEDAEERAD QAENSLSKMR SKSRASASVA PGLQSSASAA
1960
VIRSPSRARA SDF
Length:1,963
Mass (Da):224,755
Last modified:February 19, 2014 - v2
Checksum:iD653C86050E846C6
GO

Sequence cautioni

The sequence AAA28124.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1334 – 13341E → R (PubMed:7202124).Curated
Sequence conflicti1877 – 18771I → L (PubMed:7202124).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01050 Genomic DNA. Translation: AAA28124.1. Sequence problems.
Z81499, Z83107 Genomic DNA. Translation: CAB04089.1.
V01494 Genomic DNA. Translation: CAA24738.1.
PIRiT20770. MWKW.
RefSeqiNP_493596.1. NM_061195.4.
UniGeneiCel.19324.

Genome annotation databases

EnsemblMetazoaiF11C3.3.1; F11C3.3.1; WBGene00006789.
F11C3.3.2; F11C3.3.2; WBGene00006789.
F11C3.3.3; F11C3.3.3; WBGene00006789.
GeneIDi259839.
KEGGicel:CELE_F11C3.3.
UCSCiF11C3.3.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01050 Genomic DNA. Translation: AAA28124.1. Sequence problems.
Z81499, Z83107 Genomic DNA. Translation: CAB04089.1.
V01494 Genomic DNA. Translation: CAA24738.1.
PIRiT20770. MWKW.
RefSeqiNP_493596.1. NM_061195.4.
UniGeneiCel.19324.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi56866. 5 interactions.
DIPiDIP-26548N.
IntActiP02566. 6 interactions.
MINTiMINT-231086.
STRINGi6239.F11C3.3.2.

PTM databases

iPTMnetiP02566.

Proteomic databases

EPDiP02566.
PaxDbiP02566.
PRIDEiP02566.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF11C3.3.1; F11C3.3.1; WBGene00006789.
F11C3.3.2; F11C3.3.2; WBGene00006789.
F11C3.3.3; F11C3.3.3; WBGene00006789.
GeneIDi259839.
KEGGicel:CELE_F11C3.3.
UCSCiF11C3.3.1. c. elegans.

Organism-specific databases

CTDi259839.
WormBaseiF11C3.3; CE09349; WBGene00006789; unc-54.

Phylogenomic databases

eggNOGiKOG0161. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00760000118919.
HOGENOMiHOG000173959.
InParanoidiP02566.
KOiK10352.
OMAiDIKRSCW.

Miscellaneous databases

PROiP02566.

Family and domain databases

Gene3Di4.10.270.10. 1 hit.
InterProiIPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Protein structural domains in the Caenorhabditis elegans unc-54 myosin heavy chain gene are not separated by introns."
    Karn J., Brenner S., Barnett L.
    Proc. Natl. Acad. Sci. U.S.A. 80:4253-4257(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Periodic charge distributions in the myosin rod amino acid sequence match cross-bridge spacings in muscle."
    McLachlan A.D., Karn J.
    Nature 299:226-231(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 847-1963.
  4. "The genes sup-7 X and sup-5 III of C. elegans suppress amber nonsense mutations via altered transfer RNA."
    Wills N., Gesteland R.F., Karn J., Barnett L., Bolten S., Waterston R.H.
    Cell 33:575-583(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1873-1963.
  5. "A direct interaction between IP(3) receptors and myosin II regulates IP(3) signaling in C. elegans."
    Walker D.S., Ly S., Lockwood K.C., Baylis H.A.
    Curr. Biol. 12:951-956(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ITR-1.

Entry informationi

Entry nameiMYO4_CAEEL
AccessioniPrimary (citable) accession number: P02566
Secondary accession number(s): O02244
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 19, 2014
Last modified: June 8, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Miscellaneous

There are four different myosin heavy chains in C.elegans.
MHC A and MHC B are found exclusively in the body wall muscle. They co-assemble into body wall thick filament.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.